Fig. 4

Claustrum neurons projecting to the retrosplenial cortex express Nurr1 and Nr2f2 more extensively than other claustrocortical pathways. A–C Bar plots showing the mean percentage of GFP-expressing cells colocalized with Nurr1 (Nurr1⁺GFP⁺) in neurons projecting to the retrosplenial cortex (RSC), anterior cingulate cortex (ACC), primary motor cortex (MOp) and lateral entorhinal cortex (LEC) in the anterior (A), middle (B) and posterior (C) claustrum (CLA). D–F Same as A-C, but for GFP-expressing cells colocalized with Nr2f2 (Nr2f2⁺GFP⁺). G–I Same as A–C, but for GFP-expressing cells colocalized with Tle4 (Tle4⁺GFP⁺). Error bars represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; one-way ANOVA followed by Bonferroni test (see Additional file 2: Table S2 for detailed statistical analysis). RSC (n = 6 mice, 3 male and 3 female), ACC (n = 5 mice, all male), MOp (n = 4 mice, all male), LEC (n = 4 mice, all male)