Figure 1
From: Requirement of the immediate early gene vesl-1S/homer-1a for fear memory formation
Targeting construct. a, Protein structure of Vesl-1S (186 amino acids) and Vesl-1L (366 amino acids). The 175 N-terminal amino acids are shared (black box). White boxes represent sequences unique to each isoform (11 (Vesl-1S) and 191 (Vesl-1L) amino acids). EVH1; Ena-Vasp homology domain 1. b, Genomic structure of the vesl-1 gene family. Gray boxes represent genomic regions specific to each isoform. White boxes represent the 5' and 3' untranslated regions of each isoform. Black boxes represent the regions shared among the three isoform [23, 24]. The genomic structure of KO mice are also shown for comparison (bottom). Figure 1b is not in scale. E1-E11, exon 1–11. c, Targeting construct. The thick bar on the left represents the 3' region of intron 4 and the following vertical thick bar represents the exon 5 shared region. The thick bar on the right represents intron 5, downstream of the vesl-1S-specific region. Crossed lines denote the homologous recombination site. pA, SV40 poly A sequence. neor, neomycine resistant gene including promoter and bovine growth hormone poly(A). DT-A, diphtheria toxin A fragment.