Figure 2
From: Requirement of the immediate early gene vesl-1S/homer-1a for fear memory formation
Generation of short-form-specific KO mice. a, Southern blotting. HindIII (H) digestion was probed using a 5' probe (upper panel). HincII (HII) digestion was probed using a 3' probe (lower panel). b, Northern blotting. c, Western blotting. Mice were subjected to MECS and were sampled 3 or 5 h later (see Materials and Methods for details). An anti-Vesl-1L antibody (upper panels) or anti-Vesl-1S antibody (lower panel) was used for immunoblotting. Anti-Vesl-1L antibody recognized both Vesl-1L and Vesl-1L-Δ12. Arrow, Vesl-1S; arrowhead, non-specific signal that was detected by antibody we used; r-1L, recombinant Vesl-1L; r-1S, recombinant Vesl-1S.