- Short report
- Open Access
LTP induction within a narrow critical period of immature stages enhances the survival of newly generated neurons in the adult rat dentate gyrus
© Kitamura et al; licensee BioMed Central Ltd. 2010
Received: 19 March 2010
Accepted: 28 April 2010
Published: 28 April 2010
Neurogenesis occurs in the adult hippocampus of various animal species. A substantial fraction of newly generated neurons die before they mature, and the survival rate of new neurons are regulated in an experience-dependent manner. Previous study showed that high-frequency stimulation (HFS) of perforant path fibers to the hippocampal dentate gyrus (DG) induces the long-term potentiation (LTP) in the DG, and enhances the survival of newly generated neurons in the DG. In this study, we addressed whether a time period exists during which the survival of new neurons is maximally sensitive to the HFS. We found that the enhancement of cell survival by HFS was exclusively restricted to the specific narrow period during immature stages of new neurons (7-10 days after birth). Furthermore, the pharmacological blockade of LTP induction suppressed the enhancement of cell survival by the HFS. These results suggest that the LTP induction within a narrow critical period of immature stages enhances the survival of newly generated neurons in rat DG.
Large numbers of new neurons are generated throughout adulthood in the hippocampal dentate gyrus (DG) of many mammals, including rats, monkeys, and humans [1–3]. New neurons are functionally integrated into pre-existing neuronal circuits [4–7] and contribute to learning and memory , particularly the systems consolidation of memory . A subset of newly generated granule cells is selected to survive, and the others are eliminated through programmed cell death within a month [10–14]. The survival rate of new neurons is regulated in an experience-dependent manner, such as environmental enrichment, running wheel exercises, and learning contributing to survival [15–18]. Previous study shows that high-frequency stimulation (HFS) of perforant path fibers to the DG induces long-term potentiation (LTP) in the DG and enhances the survival of newly-born neurons in the DG . In this study, we addressed whether a time period exists during which the survival of new neurons is maximally sensitive to HFS. Furthermore, to understand the causal relationship between LTP induction and the survival, we used an antagonist of the N-methyl-D-aspartate (NMDA)-type glutamate receptor to inhibit the induction of LTP, and under these conditions we examined the effect of HFS on the survival rate of new neurons in the DG of unanesthetized freely moving rats.
We examined male Wistar ST rats approximately 20-25 weeks of age. All procedures involving the use of animals were conducted in compliance with the guidelines of the National Institutes of Health and were approved by the Animal Care and Use Committee of the Mitsubishi Kagaku Institute of Life Sciences.
Dentate gyrus LTP in unanesthetized freely moving rats
We used a surgical procedure described previously [9, 21–23]. Briefly, a bipolar stimulating electrode and a monopolar recording electrode made of tungsten wire were positioned stereotaxically to selectively stimulate the medial perforant pathways (MPP) and projections. The electrode stimulating the MPP fibers was positioned 8.7 mm posterior, 5.3 mm lateral, and 5.3 mm inferior to bregma. A recording electrode was implanted ipsilaterally 4.0 mm posterior, 2.5 mm lateral, and 3.8 mm inferior to bregma (Fig. 1A, B). Rats were allowed to recover for at least 2 weeks in individual home cages. Following recovery, input/output curves reflecting evoked field excitatory postsynaptic potentials (fEPSP) as a function of current intensity (0.1-1.0 mA) were collected for each rat. We then selected a current intensity that evoked 60% of the maximum population spike amplitude for all stimulations, and kept the intensity constant throughout the experiment. There was no difference in current intensity of test pulse stimulation between all experimental groups (data not shown). Test stimuli were delivered at 20-s intervals to record the fEPSP. We used strong and weak experimental stimuli. HFS (500), the strong tetanic stimulus (biphasic square wave form, 200 μs pulse width), consisted of 10 trains with 1-min intertrain intervals. Each train consisted of 5 bursts of 10 pulses at 400 Hz, delivered at 1-s interburst intervals. The weak tetanic stimulation, HFS (90), consisted of 6 trains at 10-s intertrain intervals. Each train consisted of 15 pulses at 200 Hz (biphasic square wave form, 200 μs pulse width).
All data are presented as mean ± SEM. The number of animals used is indicated by "n". Comparisons between two-group data were analysed by unpaired Student's t-test or paired t-test. Multiple group comparisons were assessed using a one-way analysis of variance (ANOVA), followed by the post-hoc Scheffe's test when significant main effects were detected. The null hypothesis was rejected at the P < 0.05 level.
Sampling region for evaluating the effect of HFS on neurogenesis in the DG
We examined LTP in the dentate gyrus of freely moving unanesthetized rats. We monitored population spike (PS) amplitudes in the DG after the delivery of HFS (Fig. 1C, D, E). When the weak HFS (90) was delivered, the potentiation of PS amplitudes persisted for 1 d but decayed to the basal level within 7 d (Fig. 1D), as described previously . In contrast, the potentiation of PS amplitudes in the DG persisted for at least 7 d when the strong HFS (500) was delivered (Fig. 1E). HFS (500) also induces the persistent increase in slope of fEPSP . To clarify the dentate area that is affected by HFS (the region where LTP may occur), we examined the expression of vesl-1S/Homer-1a mRNA. vesl-1S/Homer-1a is a neural activity-regulated gene [21, 24, 25] whose expression is positively regulated by LTP induction. We also stained sections for phalloidin, a specific probe for F-actin, as an index of LTP; LTP is accompanied by enhanced F-actin content in spines that is essential for LTP maintenance . We sampled the brain 45 min after applying HFS (500). The brain was cut by coronal section through the anterior-posterior extent of DG. We detected the expression of vesl-1S mRNA in nuclei of granule cells and the enhancement of phalloidin reactivity in the middle molecular layer (MML) in the dorsal DG area and partially in the ventral DG area (Fig. 1F). In control rats without HFS treatment, there are no detections in the expression of vesl-1S mRNA in nuclei of granule cells  and in the enhancement of phalloidin reactivity in the MML . We therefore chose the dorsal DG area (from -3.0 mm to -5.0 mm relative to bregma) as the sampling region for evaluating the effect of HFS on neurogenesis in the DG (Fig. 1B).
HFS enhances cell proliferation in the DG
LTP induction within a narrow critical period enhances cell survival in the DG
Finally, to address whether the enhancement of cell survival by HFS (500) is due to the induction of LTP within the narrow critical period, we used 3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP), which is a selective NMDA receptor antagonist, to block the induction of LTP in the perforant path-DG synapses (Fig. 3D). The injection of CPP (10 mg/kg, i.p., TOCRIS) 2 hours before HFS (500) clearly blocked the LTP induction in rat DG (Fig. 3E). We examined the effect of the pharmacological blockade of LTP induction on cell survival when HFS (500) was applied 7 d after the last BrdU injection (Fig. 3D). We observed no difference between the group with CPP injection alone and the CPP-HFS group in the R/L ratio of the number of BrdU-NeuN double-labeled cells (t-test; t13 = -0.40, P > 0.6) (Fig. 3F), indicating that injecting CPP before HFS suppressed the ability of HFS (500) to enhance the survival of newly generated neurons. CPP injection alone 7 days after the last BrdU injection did not affect cell survival in DG of control rats (no HFS treatment, measured by the number of BrdU+NeuN+ cells 28 days after last BrdU injection, CPP(-) group, 178 ± 13 cells; CPP(+) group, 158 ± 17 cells, t14 = 0.93; P > 0.3).
Consistent with previous reports [19, 26, 27], we observed that HFS (500) of perforant path fibers to the DG increases the cell proliferation in the DG. Interestingly, HFS (90), which elicited relatively short LTP that persisted for 24 hr but decayed to the basal level within 7 days, did not enhance the proliferation in the DG. Previous report shows that the stimulation protocol (288 pulse, 400 Hz), which is intermediate between the HFS (500) and HFS (90), enhances the cell proliferation in the DG . The protocol is similar to the HFS (300) (300 pulse, 400 Hz), which results in 7 days LTP . Taken together, these results suggest that the protocols of HFS which induces persistent LTP (1 week~) in the DG are required for the enhancement of cell proliferation.
We observed that HFS of perforant path fibers to the DG enhances the survival of newly born neurons in the DG, as previously reported . The effect of HFS was restricted to a specific narrow period (7-10 days after cell birth) during the immature stages of new neurons. Furthermore, this enhancement was blocked by CPP, which completely blocked the induction of LTP in the DG. These results strongly suggest that LTP induction within a narrow critical period during neuronal development enhances the survival of newly generated neurons in the adult rat DG.
How does LTP induction in the DG enhance the survival of newly generated neurons? Taking advantage of in vivo LTP paradigm, we selectively applied HFS to the medial perforant path fibers (MPP) and elicited LTP specifically at the synapses in the middle molecular layer (MML) . Under these conditions, we examined here the effect of HFS on the survival of 7-10 days-old cells in the DG. Using the same rat strain with similar ages, ca. 20-weeks old, we previously showed that the apical dendrites of 7-10 days-old cells do not reach MML nor do they have dendritic spines . These observations strongly suggest that 7-10 days-old cells do not make synapses in MML nor receive glutamatergic input from MPP. Given the timing of dendritic growth and spine formation in the MML, we speculate that HFS (500) may activate the NMDA receptors of pre-existing mature neurons, which in turn may release the secreted factors from the mature neurons to extrinsically modulate the survival of young neurons in the 7 to 10 d after birth [12, 29, 30]. Indeed, HFS (500) increased not only the number of surviving BrdU+NeuN+ cells but also the number of surviving BrdU+NeuN- cells; these are non-neuronal cells (Fig. 3), suggesting that HFS (500) may trigger the release of general survival factor(s). Activin, a member of the transforming growth factor-β superfamily, induces numerous cell differentiations and promotes cell survival . Previously, we and others found that the HFS treatment increases the level of activin β A mRNA in mature granule neurons of the DG in an NMDA receptor-dependent manner [32, 33]. Furthermore, our transgenic approach revealed that the activin signaling is crucial for the survival of newly generated neurons in the adult DG [34, 35]. Our experiments with hippocampal culture also showed that activin treatment increases the population of surviving Prox-1-positive cells at DIV21 (Prox-1 is a marker of granule cells of dentate gyrus), whereas follistatin, an inhibitor of activin signalling pathway, decreases the population . Based on these studies, the HFS-induced release of activin β A from mature granule neurons may specifically affect the immature neurons during this narrow period, and then may enhance the survival rate of new neurons in adult DG. Finally, we would like to point out a recent electrophysiological study that suggests that at 10 days after birth, small population of new cells (9.3%) begin to receive the glutamatergic input from the perforant path, . Thus, at present we do not completely exclude the possibility that HFS directly acts on the 7-10 days-old cells for survival. Further investigation is required to elucidate the mechanisms through which HFS regulates the survival of new cells.
Previous studies have shown that some form of hippocampus-dependent learning and the experiences of an enriched environment also affect the survival of new neurons at early immature stages in the DG [16–18]. Thus, there is a check point during the early immature stages of neurons that responds to experience to determine whether new neurons in the adult hippocampus would survive or die experience-dependently in later stages of development.
We thank S. Kamijo, H. Hidaka, and Y. Watanabe for maintenance of animals. We also thank all members of the Inokuchi laboratory for daily discussion, advice, and reading manuscript. This work was supported by the Core Research for Evolutional Science and Technology (CREST) program of the Japan Science and Technology Agency (JST) to K.I., a Grant-in-Aid for Scientific Research in Priority Area in "Molecular Brain Science" to K.I., and by a Grant-in-Aid for Young Scientists B to T.K.
- Altman J, Das GD: Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats. J Comp Neurol. 1965, 124: 319-335. 10.1002/cne.901240303.View ArticlePubMedGoogle Scholar
- Seki T, Arai Y: The persistent expression of a highly polysialylated NCAM in the dentate gyrus of the adult rat. Neurosci Res. 1991, 12: 503-513. 10.1016/S0168-0102(09)80003-5.View ArticlePubMedGoogle Scholar
- Eriksson PS, Perfilieva E, Bjork-Eriksson T, Alborn AM, Nordborg C, Peterson DA, Gage FH: Neurogenesis in the adult human hippocampus. Nat Med. 1998, 4: 1313-1317. 10.1038/3305.View ArticlePubMedGoogle Scholar
- van Praag H, Schinder AF, Christie BR, Toni N, Palmer TD, Gage FH: Functional neurogenesis in the adult hippocampus. Nature. 2002, 415: 1030-1034. 10.1038/4151030a.View ArticlePubMedGoogle Scholar
- Schmidt-Hieber C, Jonas P, Bischofberger J: Enhanced synaptic plasticity in newly generated granule cells of the adult hippocampus. Nature. 2004, 429: 184-187. 10.1038/nature02553.View ArticlePubMedGoogle Scholar
- Kee N, Teixeira CM, Wang AH, Frankland PW: Preferential incorporation of adult-generated granule cells into spatial memory networks in the dentate gyrus. Nat Neurosci. 2007, 10: 355-362. 10.1038/nn1847.View ArticlePubMedGoogle Scholar
- Toni N, Teng EM, Bushong EA, Aimone JB, Zhao C, Consiglio A, van Praag H, Martone ME, Ellisman MH, Gage FH: Synapse formation on neurons born in the adult hippocampus. Nat Neurosci. 2007, 10: 727-734. 10.1038/nn1908.View ArticlePubMedGoogle Scholar
- Zhao C, Deng W, Gage FH: Mechanisms and functional implications of adult neurogenesis. Cell. 2008, 132: 645-660. 10.1016/j.cell.2008.01.033.View ArticlePubMedGoogle Scholar
- Kitamura T, Saitoh Y, Takashima N, Murayama A, Niibori Y, Ageta H, Sekiguchi M, Sugiyama H, Inokuchi K: Adult neurogenesis modulates the hippocampus-dependent period of associative fear memory. Cell. 2009, 139: 814-827. 10.1016/j.cell.2009.10.020.View ArticlePubMedGoogle Scholar
- Cameron HA, McKay RD: Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus. J Comp Neurol. 2001, 435: 406-417. 10.1002/cne.1040.View ArticlePubMedGoogle Scholar
- Kempermann G, Gast D, Kronenberg G, Yamaguchi M, Gage FH: Early determination and long-term persistence of adult-generated new neurons in the hippocampus of mice. Development. 2003, 130: 391-399. 10.1242/dev.00203.View ArticlePubMedGoogle Scholar
- Kitamura T, Mishina M, Sugiyama H: Enhancement of neurogenesis by running wheel exercises is suppressed in mice lacking NMDA receptor epsilon 1 subunit. Neurosci Res. 2003, 47: 55-63. 10.1016/S0168-0102(03)00171-8.View ArticlePubMedGoogle Scholar
- Tashiro A, Sandler VM, Toni N, Zhao C, Gage FH: NMDA-receptor-mediated, cell-specific integration of new neurons in adult dentate gyrus. Nature. 2006, 442: 929-933. 10.1038/nature05028.View ArticlePubMedGoogle Scholar
- Sun W, Winseck A, Vinsant S, Park OH, Kim H, Oppenheim RW: Programmed cell death of adult-generated hippocampal neurons is mediated by the proapoptotic gene Bax. J Neurosci. 2004, 24: 11205-11213. 10.1523/JNEUROSCI.1436-04.2004.View ArticlePubMedGoogle Scholar
- Kempermann G, Kuhn HG, Gage FH: More hippocampal neurons in adult mice living in an enriched environment. Nature. 1997, 386: 493-495. 10.1038/386493a0.View ArticlePubMedGoogle Scholar
- Gould E, Beylin A, Tanapat P, Reeves A, Shors TJ: Learning enhances adult neurogenesis in the hippocampal formation. Nat Neurosci. 1999, 2: 260-265. 10.1038/6365.View ArticlePubMedGoogle Scholar
- Tashiro A, Makino H, Gage FH: Experience-specific functional modification of the dentate gyrus through adult neurogenesis: a critical period during an immature stage. J Neurosci. 2007, 27: 3252-3259. 10.1523/JNEUROSCI.4941-06.2007.View ArticlePubMedGoogle Scholar
- Dupret D, Fabre A, Dobrossy MD, Panatier A, Rodriguez JJ, Lamarque S, Lemaire V, Oliet SH, Piazza PV, Abrous DN: Spatial learning depends on both the addition and removal of new hippocampal neurons. PLoS Biol. 2007, 5: e214-10.1371/journal.pbio.0050214.PubMed CentralView ArticlePubMedGoogle Scholar
- Bruel-Jungerman E, Davis S, Rampon C, Laroche S: Long-term potentiation enhances neurogenesis in the adult dentate gyrus. J Neurosci. 2006, 26: 5888-5893. 10.1523/JNEUROSCI.0782-06.2006.View ArticlePubMedGoogle Scholar
- Hayashi F, Takashima N, Murayama A, Inokuchi K: Decreased postnatal neurogenesis in the hippocampus combined with stress experience during adolescence is accompanied by an enhanced incidence of behavioral pathologies in adult mice. Mol Brain. 2008, 1: 22-10.1186/1756-6606-1-22.PubMed CentralView ArticlePubMedGoogle Scholar
- Kato A, Ozawa F, Saitoh Y, Hirai K, Inokuchi K: vesl, a gene encoding VASP/Ena family related protein, is upregulated during seizure, long-term potentiation and synaptogenesis. FEBS Lett. 1997, 412: 183-189. 10.1016/S0014-5793(97)00775-8.View ArticlePubMedGoogle Scholar
- Fukazawa Y, Saitoh Y, Ozawa F, Ohta Y, Mizuno K, Inokuchi K: Hippocampal LTP is accompanied by enhanced F-actin content within the dendritic spine that is essential for late LTP maintenance in vivo. Neuron. 2003, 38: 447-460. 10.1016/S0896-6273(03)00206-X.View ArticlePubMedGoogle Scholar
- Okada D, Ozawa F, Inokuchi K: Input-specific spine entry of soma-derived Vesl-1S protein conforms to synaptic tagging. Science. 2009, 324: 904-909. 10.1126/science.1171498.View ArticlePubMedGoogle Scholar
- Brakeman PR, Lanahan AA, O'Brien R, Roche K, Barnes CA, Huganir RL, Worley PF: Homer: a protein that selectively binds metabotropic glutamate receptors. Nature. 1997, 386: 284-288. 10.1038/386284a0.View ArticlePubMedGoogle Scholar
- Inoue N, Nakao H, Migishima R, Hino T, Matsui M, Hayashi F, Nakao K, Manabe T, Aiba A, Inokuchi K: Requirement of the immediate early gene vesl-1S/homer-1a for fear memory formation. Mol Brain. 2009, 2: 7-10.1186/1756-6606-2-7.PubMed CentralView ArticlePubMedGoogle Scholar
- Derrick BE, York AD, Martinez JL: Increased granule cell neurogenesis in the adult dentate gyrus following mossy fiber stimulation sufficient to induce long-term potentiation. Brain Res. 2000, 857: 300-307. 10.1016/S0006-8993(99)02464-6.View ArticlePubMedGoogle Scholar
- Chun SK, Sun W, Park JJ, Jung MW: Enhanced proliferation of progenitor cells following long-term potentiation induction in the rat dentate gyrus. Neurobiol Learn Mem. 2006, 86: 322-329. 10.1016/j.nlm.2006.05.005.View ArticlePubMedGoogle Scholar
- Ohkawa N, Saitoh Y, Tokunaga E, Kitamura T, Inokuchi K: Spine formation pattern of new neurons is modulated by induction of long-term potentiation (LTP) in adult dentate gyrus. Society for Neuroscience. 2009, 408.7/A7:Google Scholar
- Udo H, Yoshida Y, Kino T, Ohnuki K, Mizunoya W, Mukuda T, Sugiyama H: Enhanced adult neurogenesis and angiogenesis and altered affective behaviors in mice overexpressing vascular endothelial growth factor 120. J Neurosci. 2008, 28: 14522-14536. 10.1523/JNEUROSCI.3673-08.2008.View ArticlePubMedGoogle Scholar
- Ma DK, Jang MH, Guo JU, Kitabatake Y, Chang ML, Pow-Anpongkul N, Flavell RA, Lu B, Ming GL, Song H: Neuronal activity-induced Gadd45b promotes epigenetic DNA demethylation and adult neurogenesis. Science. 2009, 323: 1074-1077. 10.1126/science.1166859.PubMed CentralView ArticlePubMedGoogle Scholar
- Schubert D, Kimura H, LaCorbiere M, Vaughan J, Karr D, Fischer WH: Activin is a nerve cell survival molecule. Nature. 1990, 344: 868-870. 10.1038/344868a0.View ArticlePubMedGoogle Scholar
- Inokuchi K, Kato A, Hiraia K, Hishinuma F, Inoue M, Ozawa F: Increase in activin beta A mRNA in rat hippocampus during long-term potentiation. FEBS Lett. 1996, 382: 48-52. 10.1016/0014-5793(96)00135-4.View ArticlePubMedGoogle Scholar
- Andreasson K, Worley PF: Induction of beta-A activin expression by synaptic activity and during neocortical development. Neuroscience. 1995, 69: 781-796. 10.1016/0306-4522(95)00245-E.View ArticlePubMedGoogle Scholar
- Ageta H, Murayama A, Migishima R, Kida S, Tsuchida K, Yokoyama M, Inokuchi K: Activin in the brain modulates anxiety-related behavior and adult neurogenesis. PLoS ONE. 2008, 3: e1869-10.1371/journal.pone.0001869.PubMed CentralView ArticlePubMedGoogle Scholar
- Ageta H, Ikegami S, Miura M, Masuda M, Migishima R, Hino T, Takashima N, Murayama A, Sugino H, Setou M: Activin plays a key role in the maintenance of long-term memory and late-LTP. Learn Mem. 2010, 17: 176-185. 10.1101/lm.16659010.View ArticlePubMedGoogle Scholar
- Sekiguchi M, Hayashi F, Tsuchida K, Inokuchi K: Neuron type-selective effects of activin on development of the hippocampus. Neurosci Lett. 2009, 452: 232-237. 10.1016/j.neulet.2009.01.074.View ArticlePubMedGoogle Scholar
- Ambrogini P, Cuppini R, Lattanzi D, Ciuffoli S, Frontini A, Fanelli M: Synaptogenesis in adult-generated hippocampal granule cells is affected by behavioral experiences. Hippocampus. 2009Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.