Figure 1

Effects of Math5 -null mutation on retinal cell types. (A-H) Retinal sections from P28 were immunolabeled with cell type-specific markers (green) and nuclear counterstained with propidium iodide (PI, red). Loss of Math5 leads to an increase in the displaced amacrine cells immunoreactive for BHLHB5 (A), PROX1 (B) and GAD65 (C) in the GCL, and to a severe loss of calretinin+ (D) and ISL1+ (E) cells in the INL and the GCL. Furthermore, in the ONL, there is a marked reduction in VSX1+ cone bipolar cells (F) and rhodopsin⁺ rod photoreceptors (G), whereas the RXRγ+ cone photoreceptors are increased (H). (I) Quantification of cells expressing specific markers in the ONL, INL and the GCL per 350 μm length of retinal sections. Eyes from five mice were analyzed per genotype. Arrows in the insets indicate cells expressing specific markers in the wild type retina, while arrowheads in the insets indicate those of Math5-null retina. INL, inner nuclear layer. GCL, ganglion cell layer. ONL, outer nuclear layer. Scale bar equals 100 μm.