- Open Access
GPCR oligomers in pharmacology and signaling
Molecular Brain volume 4, Article number: 20 (2011)
G protein-coupled receptors (GPCRs) represent one of the largest families of cell surface receptors, and are the target of more than half of the current therapeutic drugs on the market. When activated by an agonist, the GPCR undergoes conformational changes that facilitate its interaction with heterotrimeric G proteins, which then relay signals to downstream intracellular effectors. Although GPCRs were thought to function as monomers, many studies support the hypothesis that G protein coupling involves the formation of GPCR homo- and/or hetero-complexes. These complex systems have been suggested to exhibit specific signaling cascades, pharmacological, internalization, and recycling properties. In this review, we summarize recent advances in our understanding of the structure, function and dynamics of GPCR complexes, as well as the findings obtained in animal models.
G protein-coupled receptors (GPCRs) , also known as seven transmembrane receptors, were initially considered to function as monomeric structural units. In the last decade, however, there has been an increasing number of publications describing GPCR homodimers or even higher order oligomers [2–5]. There is also evidence that GPCR heterocomplexes may exist [6–8]. Two of the main lines of research in structure and function of GPCRs are focused on the residues and domains responsible for GPCR complex formation, as well as the stability and mechanisms of signaling crosstalk between the components of these homo- and heteromers. Another point of interest is the identification of GPCR complexes in native tissues and their functional significance in whole animal models. In this review, we will summarize recent advances in our understanding of GPCR homo- and heteromerization, as well as their potential implication in physiological responses.
History of GPCR complexes
In the 1980s, two laboratories at the Karolinska Institute in Stockholm and the National Institute for Medical Research in London proposed the existence of receptor mosaics [9, 10] and a direct interaction of two receptors with each other . This hypothesis was also supported by previous publications demonstrating negatively cooperative site-site interactions among the β-adrenergic receptors . Similarly, using a photoaffinity labeling approach, Sokolovsky and collaborators proposed that muscarinic receptors exist in interconvertible dimer and tetramer forms . They also suggested a model in which muscarinic receptor monomers formed dimers and tetramers joined by covalent bonds; although this model of covalent bonding has not been confirmed. Immunoaffinity chromatography approaches with the β2-adrenergic receptor in mammalian lung , and protocols of radiation inactivation with the α2-adrenergic receptor in human platelets  also pointed toward the phenomenon of GPCR oligomerization. Years earlier, it had been introduced the concept that antagonists bind to the receptor with one uniform affinity whereas, for agonists, two distinct binding sites of high and low affinity are detected . They demonstrated that the proportion of agonist high affinity binding sites was dramatically decreased in the presence of guanine nucleotides, and proposed the ternary complex model to explain these findings . In the 1990s, additional studies based on radioligand binding displacement curves obtained with muscarinic  and dopamine D2  receptors were consistent with the idea that the tested ligands differentially bind monomeric and dimeric GPCRs. Potter and colleagues found that competition curves between different agonists and antagonists with the muscarinic receptor antagonist N-[3H]methylscopolamine ([3H]NMS) showed the presence of equal populations of guanine nucleotide-sensitive high affinity sites (H) and guanine nucleotide-insensitive low affinity sites (L) in membrane preparations from rat brainstem . These two binding sites could be blocked independently using irreversible ligands, and the specific blockage of either H or L sites did not lead to changes to the remaining binding sites. This phenomenon, which the authors termed 50% H, was interpreted as evidence of M2 muscarinic receptor dimerization. Seeman and collaborators compared the binding properties of the dopamine D2 antagonists [3H]emonapride and [3H]spiperone in tissue cultures and in postmortem human caudate nucleus . They found that the density of D2 receptors labeled with [3H]emonapride was twice higher than that labeled with [3H]spiperone. Similar findings were obtained by positron emission tomography (PET) in human brain when using [11C]raclopride and [11C]spiperone . They proposed that [3H]spiperone preferentially binds dopamine D2 receptor dimers, whereas [3H]emonapride only binds to receptor monomers. These and other observations led the authors to speculate that GPCRs might be expressed and function as dimers or oligomers. However, the existence of GPCR oligomeric structures did not attract much attention, and the most accepted model was the one proposing that a single GPCR is sufficient to interact with a single G protein.
It was not until the findings by Wess that the concept of GPCR complex formation gained interest among the research field . They tested the functional response induced by chimeras of the muscarinic M3 and α2C-adrenergic receptors. Chimeric M3/α2C receptor was composed of the first five transmembrane domains (including intracellular loop 3) of the muscarinic M3 receptor and the last two transmembrane domains of the α2C-adrenergic receptor. Chimeric α2C/M3 receptor was composed of the first five transmembrane domains α2C-adrenergic receptor and the last two transmembrane domains (including intracellular loop 3) of the muscarinic M3 receptor.
Intracellular loop 3 of the muscarinic M3 receptor was maintained unchanged in both chimeras because this region is responsible for the specificity of G protein coupling. The signaling induced by the muscarinic receptor agonist carbachol was abolished when either of these two chimeras was expressed alone. However, coexpression of M3/α2C and α2C/M3 receptor chimeras rescued the carbachol-induced signaling, which provided the first indirect evidence for GPCR dimerization.
Two of the key findings to indicate that GPCR complexes might be important in receptor signaling were also the assembly of GABAB receptor subtypes (GABAB-R1 and GABAB-R2) into heteromeric complexes (see  for review), and the heteromerization of δ-and κ-opioid receptors affecting ligand binding and receptor signaling . Since then, several laboratories have reported the presence of GPCRs as homo- and heteromers, a concept that it is now widely accepted.
Protein domains responsible for GPCR complex formation
Much evidence indicates that class C GPCRs, including GABAB and metabotropic glutamate (mGlu) receptors, exist and function as constitutive dimers. Heterodimerization of GABAB receptor is mediated by parallel coiled-coil interactions at C-termini [24–26]. Co-expression of GABAB-R1 and GABAB-R2 results in the functional expression of GABAB receptor in the plasma membrane . Recently, it has also been shown that the mGlu receptors are assembled into strict dimers covalently bound via a disulfide bridge . Closure of both binding domains is necessary to obtain full activity of the mGlu receptors . In contrast, most of the experiments conducted on class A GPCRs suggest that receptor oligomers are formed through non-covalent interactions, with the transmembrane (TM) domains as the most likely elements involved in receptor oligomerization. We will summarize our current knowledge regarding the TM domains that form the interface of GPCR homo- and hetero-complexes.
Milligan and colleagues demonstrated using co-immunoprecipitation and fluorescence resonance energy transfer (FRET) assays that the α1b-adrenergic receptor forms oligomeric complexes in which symmetrical contact points involving both TM1 and TM4 were detected . These experiments were performed using epitope-tagged fragments comprising distinct TM domains. They also found with the use of three-color FRET (3-FRET) that the α1b-adrenergic receptor is able to form oligomeric complexes comprising at least three monomers . The 3-FRET signal was significantly reduced by a combination of single point mutations of hydrophobic residues in TM4, findings that were also associated with protein maturation and signaling function.
A similar conclusion has been reached with the dopamine D2 receptor. Thus, cysteine cross-linking experiments provided evidence for the involvement of TM1 and TM4 at the symmetrical interface of the dopamine D2 receptor oligomer, and the cross-linking was differentially affected by the presence of dopamine D2 agonists or inverse agonists [31, 32]. The combination of bioluminescence/fluorescence complementation and energy transfer approaches also suggested that the dopamine D2 receptor form higher order oligomers in the plasma membrane . They propose a dopamine D2 receptor oligomer with a symmetric interface formed by TM1 and the cytoplasmic helical domain (H8) in addition to the TM4 interface.
Serotonin 5-HT2A receptor and mGlu2 receptor form a functional heterocomplex that has been implicated in schizophrenia and psychosis [34, 35]. The differences in the capacity of the mGlu2 and mGlu3 receptors to exist as a receptor complex with the 5-HT2A receptor provided the basis to identify the specific mGlu2 receptor TM domains responsible for GPCR heterocomplex formation . Study of a series of molecular chimeras suggested that the segment containing TM4 and TM5 of the mGlu2 receptor was both necessary and sufficient for complex formation with the 5-HT2A receptor. The mGlu3 receptor chimera containing only this segment from the mGlu2 receptor (mGlu3ΔTM4-5) was capable of co-immunoprecipitating and maintaining close proximity with the 5-HT2A receptor as indicated by FRET. In contrast, the chimera mGlu2 ΔTM4-5 did not show evidence of complex formation with the 5-HT2A receptor.
Although all these findings support a contribution of TM4 and TM1 to the formation of GPCR complexes, the discrete structural components that affect this phenomenon have not been unraveled.
Stability of GPCR oligomers
One question to be solved is the stability and lifetime of the GPCR complexes in the plasma membrane. Although GPCR oligomerization has been proposed to be necessary for effective receptor maturation and surface delivery [30, 36], recent findings also suggest that GPCRs exist in a dynamic equilibrium between monomeric and dimeric/oligomeric states. Thus, using a fluorescence recovery after photobleaching (FRAP) approach, Bunemann and collaborators demonstrated that the β1-adrenergic receptor shows transients interactions on a timescale of seconds . However, for the β2-adrenergic receptor, they found stable oligomers in HEK293 cells and cardiac myocytes. Similar findings using FRAP were obtained in studies examining the stability of dopamine D2 receptors, with which they demonstrated a dynamic equilibrium between monomer and homodimer/oligomer states of the dopamine D2 receptor .
The dynamic nature of dimer formation of muscarinic M1 receptor has been recently detailed by using total internal reflection fluorescence microscopy (TIRFM) in living cells . They visualized single muscarinic M1 receptors and found that they exist as monomers and dimers, but not oligomers. They also found that ~30% of the receptor molecules exist as a dimer at any given time, and suggested that muscarinic M1 receptors do not form constitutive dimers.
Several studies have demonstrated that GPCR heteromerization affects the pharmacological and cellular signaling responses as compared to the different receptor subtypes expressed alone. One well-studied example is the receptor heterocomplex between the α2a-adrenergic receptor and the μ-opioid receptor. α2a-Adrenergic receptor and μ-opioid receptor have been repeatedly involved in depression and opioid addiction [40–44]. Direct interaction between α2a-adrenergic and μ-opioid receptors was demonstrated in tissue cultures and primary neurons . Using this GPCR heterocomplex as a model, Vilardaga and Lohse reported that α2a-adrenergic and μ-opioid receptors communicate one with each other through the receptor complex . They found that morphine binding to the μ-opioid receptor induces structural changes in the α2a-adrenergic receptor when this is bound to norepinephrine. Previous findings showed that whereas activation of either α2a-adrenergic receptor or μ-opioid receptor increases phosphorilation of ERK 1/2 in HEK293 cells and in primary spinal cord neurons, activation of both receptors leads to a decrease signal activation . Using FRET microscopy, it was found that the conformational changes induced in the α2a-adrenergic receptor by morphine through the receptor heterocomplex inhibit G protein coupling and the ERK signaling pathway .
Another indication of GPCR heterocomplexes that affect G protein coupling comes from studies on interactions between 5-HT2A and mGlu2 receptors. The 5-HT2A receptor and the mGlu2 receptor are usually coupled to Gq/11 and Gi/o proteins, respectively. We recently reported that hallucinogenic 5-HT2A receptor agonists activate both Gq/11 and Gi/o proteins only when the 5-HT2A receptor forms a receptor heterocomplex with the mGlu2 receptor [34, 47]. These findings have been further supported in vivo in murine models. The head-twitch is a behavioral response that is reliably elicited by hallucinogens, and is absent in 5-HT2A-KO mice . We found that head-twitch response is abolished in mGlu2-KO mice , which suggests that the 5-HT2A-mGlu2 receptor complex is necessary for the behavioral responses induced by hallucinogenic 5-HT2A receptor agonists (Figure 1).
Although these and other findings support the functional and behavioral significance of GPCR heterocomplexes, many others demonstrate functional interactions between GPCR subtypes that do not require formation of GPCR complexes. It was reported that the GABAB receptor potentiates the mGlu1 receptor-dependent signaling, yet these two receptors do not associate with each other . Similarly, it has been shown that the corticotropin-releasing factor 1 (CRF1) receptor modulates G protein coupling and anxiety-like behaviors by sensitizing 5-HT2A receptor-dependent signaling . The mechanism of interaction between CRF1 and 5-HT2A receptors is not direct, and requires scaffolding proteins.
GPCR oligomers in native tissues
All the findings described above suggest the existence of GPCR homo- and hetero-complexes in tissue cultures. Similar findings have been obtained with GPCRs reconstituted in lipid vesicles . A fundamental question to be addressed is the presence of GPCR complexes in native tissue. Three recent publications using different approaches support this hypothesis.
Reproduction in animals is dependent on the pulsatile release of pituitary gonadotropic hormones: luitenizing hormone (LH) and follicle-stimulating hormone (FSH). In the testis, LH acts through LH receptors (LHR) on the Leydig cells to regulate the production and secretion of androgens. Biological actions of LH and FSH also include maturation of testis and ovary . Huhtaniemi and colleagues developed transgenic mice expressing mutant LH receptors (LHR) in the absence of functional endogenous receptor (i.e., in LHR-KO mice) . They selected two mutant LHRs that were not able to bind the ligand or to modulate G protein coupling by agonist activation. The first mutant LHR (Cys22Ala) is expressed on the cell surface but is incapable of binding the LHR agonist hCG . The second mutant harbors a deletion (Val553 to Ala689) that abolishes G protein coupling and cellular signaling . They confirmed in tissue cultures that the first mutant (LHRLH-) did not bind hCG and that the second mutant (LHRcAMP-) did not activate cAMP production. Co-expression of LHRLH- and LHRcAMP- partially rescued the cAMP response to hCG stimulation in HEK293 cells . Similar findings for agonist binding and G protein coupling were also observed in the testes of LHRLH- and LHRcAMP- mutant mice. They also found that both transgenic mice showed a phenotype similar to that of LHR-KO mice. This phenotype was rescued in mice expressing both LHRLH- and LHRcAMP- receptors. The testes were normally developed, and transgenic mice expressing both LHRLH- and LHRcAMP- showed serum hormone levels comparable to those seen in wild type mice. The authors proposed intermolecular cooperation in vivo as a potential mechanism to understand the physiological relevance of these findings, and suggested GPCR oligomerization as the biophysical basis of these specific interactions.
Co-immunoprecipitation assays represent one of the most commonly used approaches to validate in vivo the significance of findings with GPCR heterocomplexes obtained in tissue cultures. This assay suggests that GPCRs might form heterocomplexes in native tissue. Another possibility is that GPCRs form part of the same protein complex, yet they interact indirectly through scaffolding proteins, and are not in close proximity at the plasma membrane. An essential tool to better define the existence of close proximity is the development of antibodies that only react when the two components of the GPCR heterocomplex are co-expressed in the same cell, and not when they are expressed alone. Using a strategy of subtractive immunization, Devi and collaborators developed monoclonal antibodies to selectively recognize the μ-δ opioid receptor heteromer, but not either μ-opioid receptor or δ-opioid receptor, in the central nervous system . They detected μ-δ opioid receptor heteromers in cortical membranes of wild type mice, using μ-KO, δ-KO and μ-δ-KO mice as negative controls. Chronic, but not acute, treatment with morphine induced an increase in the expression of μ- and δ-opioid receptors as a GPCR heterocomplex in brain regions involved in drug addiction such as frontal cortex, striatum and nucleus accumbens.
An interesting approach is the use of FRET with fluorescent ligands in native tissue. Albizu and Durroux optimized the experimental conditions to perform homogenous time-resolved FRET (HTRF) experiments with the use of fluorescent ligands . HTRF is based on the derivation of GPCR ligands with a lanthanide and a compatible fluorophore. The authors previously generated fluorescent ligands for vasopressin and oxytocin receptors , which are highly expressed in mammary glands. Using fluorescent ligands, they obtained FRET signal with native oxytocin receptors in rat mammary glands . Experiments revealing GPCR complexes were performed both in membrane preparations and in patches of native tissues. Taken together, these findings suggest that GPCR are placed in close proximity in vivo in murine models.
Conclusions and Future Directions
The functional crosstalk observed in between the components of GPCR heterocomplexes provides a unique scenario for the rational design of new therapeutic drugs. Examples of this include the 5-HT2A-mGlu2 receptor complex in schizophrenia , the μ-opioid-α2a-adrenergic receptor complex in depression and opioid addiction , the adenosine A2A-dopamine D2 receptor complex in Parkinson's disease , and the κ-δ-opioid receptor complex in analgesia and drug abuse . However, the majority of findings suggesting the expression of these GPCRs as homo- and hetero-oligomers have been reported using biochemical, biophysical and pharmacological approaches in tissue cultures, and not in rodents. Only recently we have started to make progress toward the ultimate goal of demonstrating the physiological and behavioral relevance of GPCR heterocomplexes in whole animal models. A tempting approach to validate their significance is the use of knock-in mice that express two GPCRs as functional homomers, but not as heteromers.
Unraveling the residues and domains that form part of the GPCR complex interface might provide the basis for the design of drugs that specifically affect the signaling crosstalk between the components of the receptor complex. Most of the findings obtained in tissue cultures support the hypothesis that TM1 or TM4 and 5 form the interfaces between GPCR monomers to form higher order oligomers. However, crystal structures of the CXCR4 chemokine receptor provide evidence for a symmetric dimer in which monomers interact only at the extracellular side of TM5 and TM6 . These findings are different from previous observations, and make clear the need for further research in the field. Interestingly, the crystallization of rhodopsin revealed an antiparallel dimer with respect to the plane of the membrane , most probably due to the effects of solubilization protocols on the physiologically relevant dimers.
Recent breakthroughs in the determination of the crystal structures of GPCRs have provided indirect information regarding G protein coupling. Comparison of the inactive and active  structures of the β2-adrenergic receptor suggests that the differences are mainly induced at the cytoplasmic region of the receptor, with outward displacement of TM5 and TM6 and inward movement of TM7 and TM3 in the active relative to the inactive structure. In contrast, only small differences were observed between the two protein structures in the agonist-binding pocket. Heterotrimeric G proteins are composed of three subunits: Gα, Gβ and γ . Recent findings based on systematic cross-linking experiments have identified many of the contact sites between muscarinic M3 receptor and Gαq subunit . In addition, Gβ and γ also play a key role in determining receptor-G protein specificity . Since simultaneous interaction of the receptor with both Gα and Gβγ subunits is required for G protein activation, and based on the size of the intracellular region of the receptor exposed to the cytoplasm, it is tempting to speculate that a single receptor might show limited coupling to the heterotrimeric G protein. Although this hypothesis is supported by the development of a functional complementation assay that points to two receptors and a single G protein as the minimal signaling unit of the dopamine D2 receptor , it has also been shown that rhodopsin  and the β2-adrenergic receptor  signal efficiently to G proteins when reconstituted into lipid nanodiscs containing a single receptor. Further studies will be needed to better understand the complexity of receptor-G protein coupling [70, 71], and the role of GPCR homo-and hetero-complexes in receptor signaling. Despite the advances in our knowledge of structure and function of GPCRs, much more needs to be learned about the pharmacology, neuroanatomy and signaling of GPCR oligomers both in vitro and in mouse models before developing new therapeutic drugs that affect their function.
Rosenbaum DM, Rasmussen SG, Kobilka BK: The structure and function of G-protein-coupled receptors. Nature. 2009, 459: 356-363. 10.1038/nature08144.
Milligan G: The role of dimerisation in the cellular trafficking of G-protein-coupled receptors. Curr Opin Pharmacol. 10: 23-29.
Pin JP, Neubig R, Bouvier M, Devi L, Filizola M, Javitch JA, Lohse MJ, Milligan G, Palczewski K, Parmentier M, Spedding M: International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the recognition and nomenclature of G protein-coupled receptor heteromultimers. Pharmacol Rev. 2007, 59: 5-13. 10.1124/pr.59.1.5.
Ferre S, Baler R, Bouvier M, Caron MG, Devi LA, Durroux T, Fuxe K, George SR, Javitch JA, Lohse MJ, Mackie K, Milligan G, Pfleger KD, Pin JP, Volkow ND, Waldhoer M, Woods AS, Franco R: Building a new conceptual framework for receptor heteromers. Nat Chem Biol. 2009, 5: 131-134. 10.1038/nchembio0309-131.
Birdsall NJ: Class A GPCR heterodimers: evidence from binding studies. Trends Pharmacol Sci. 31: 499-508.
Milligan G: G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function. Br J Pharmacol. 2009, 158 (1): 5-14. 10.1111/j.1476-5381.2009.00169.x.
Albizu L, Moreno JL, Gonzalez-Maeso J, Sealfon SC: Heteromerization of G protein-coupled receptors: relevance to neurological disorders and neurotherapeutics. CNS Neurol Disord Drug Targets. 9: 636-650.
Rozenfeld R, Devi LA: Receptor heteromerization and drug discovery. Trends Pharmacol Sci. 31: 124-130.
Agnati LF, Fuxe K, Zini I, Lenzi P, Hokfelt T: Aspects on receptor regulation and isoreceptor identification. Med Biol. 1980, 58: 182-187.
Agnati LF, Fuxe K, Zoli M, Rondanini C, Ogren SO: New vistas on synaptic plasticity: the receptor mosaic hypothesis of the engram. Med Biol. 1982, 60: 183-190.
Birdsall NJ: Can different receptors interact directly with each other?. Trends Neurosci. 1982, 5: 137-138.
Limbird LE, Meyts PD, Lefkowitz RJ: Beta-adrenergic receptors: evidence for negative cooperativity. Biochem Biophys Res Commun. 1975, 64: 1160-1168. 10.1016/0006-291X(75)90815-3.
Avissar S, Amitai G, Sokolovsky M: Oligomeric structure of muscarinic receptors is shown by photoaffinity labeling: subunit assembly may explain high- and low-affinity agonist states. Proc Natl Acad Sci USA. 1983, 80: 156-159. 10.1073/pnas.80.1.156.
Fraser CM, Venter JC: The size of the mammalian lung beta 2-adrenergic receptor as determined by target size analysis and immunoaffinity chromatography. Biochem Biophys Res Commun. 1982, 109: 21-29. 10.1016/0006-291X(82)91560-1.
Venter JC, Schaber JS, U'Prichard DC, Fraser CM: Molecular size of the human platelet alpha 2-adrenergic receptor as determined by radiation inactivation. Biochem Biophys Res Commun. 1983, 116: 1070-1075. 10.1016/S0006-291X(83)80251-4.
Kent RS, De Lean A, Lefkowitz RJ: A quantitative analysis of beta-adrenergic receptor interactions: resolution of high and low affinity states of the receptor by computer modeling of ligand binding data. Mol Pharmacol. 1980, 17: 14-23.
De Lean A, Stadel JM, Lefkowitz RJ: A ternary complex model explains the agonist-specific binding properties of the adenylate cyclase-coupled beta-adrenergic receptor. J Biol Chem. 1980, 255: 7108-7117.
Potter LT, Ballesteros LA, Bichajian LH, Ferrendelli CA, Fisher A, Hanchett HE, Zhang R: Evidence of paired M2 muscarinic receptors. Mol Pharmacol. 1991, 39: 211-221.
Seeman P, Guan HC, Civelli O, Van Tol HH, Sunahara RK, Niznik HB: The cloned dopamine D2 receptor reveals different densities for dopamine receptor antagonist ligands. Implications for human brain positron emission tomography. Eur J Pharmacol. 1992, 227: 139-146. 10.1016/0922-4106(92)90121-B.
Farde L, Wiesel FA, Stone-Elander S, Halldin C, Nordstrom AL, Hall H, Sedvall G: D2 dopamine receptors in neuroleptic-naive schizophrenic patients. A positron emission tomography study with [11C]raclopride. Arch GenPsychiatry. 1990, 47: 213-219.
Maggio R, Vogel Z, Wess J: Coexpression studies with mutant muscarinic/adrenergic receptors provide evidence for intermolecular "cross-talk" between G-protein-linked receptors. Proc Natl Acad Sci USA. 1993, 90: 3103-3107. 10.1073/pnas.90.7.3103.
Bowery NG, Enna SJ: gamma-aminobutyric acid(B) receptors: first of the functional metabotropic heterodimers. J Pharmacol Exp Ther. 2000, 292: 2-7.
Jordan BA, Devi LA: G-protein-coupled receptor heterodimerization modulates receptor function. Nature. 1999, 399: 697-700. 10.1038/21441.
Calver AR, Robbins MJ, Cosio C, Rice SQ, Babbs AJ, Hirst WD, Boyfield I, Wood MD, Russell RB, Price GW, Couve A, Moss SJ, Pangalos MN: The C-terminal domains of the GABA(b) receptor subunits mediate intracellular trafficking but are not required for receptor signaling. J Neurosci. 2001, 21: 1203-1210.
Margeta-Mitrovic M, Jan YN, Jan LY: Function of GB1 and GB2 subunits in G protein coupling of GABA(B) receptors. Proc Natl Acad Sci USA. 2001, 98: 14649-14654. 10.1073/pnas.251554498.
Kammerer RA, Frank S, Schulthess T, Landwehr R, Lustig A, Engel J: Heterodimerization of a functional GABAB receptor is mediated by parallel coiled-coil alpha-helices. Biochemistry. 1999, 38: 13263-13269. 10.1021/bi991018t.
Maurel D, Comps-Agrar L, Brock C, Rives ML, Bourrier E, Ayoub MA, Bazin H, Tinel N, Durroux T, Prezeau L, Trinquet E, Pin JP: Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies:application to GPCR oligomerization. Nat Methods. 2008, 5: 561-567. 10.1038/nmeth.1213.
Kniazeff J, Bessis AS, Maurel D, Ansanay H, Prezeau L, Pin JP: Closed state of both binding domains of homodimeric mGlu receptors is required for full activity. Nat Struct Mol Biol. 2004, 11: 706-713. 10.1038/nsmb794.
Carrillo JJ, Lopez-Gimenez JF, Milligan G: Multiple interactions between transmembrane helices generate the oligomeric alpha1b-adrenoceptor. Mol Pharmacol. 2004, 66: 1123-1137. 10.1124/mol.104.001586.
Lopez-Gimenez JF, Canals M, Pediani JD, Milligan G: The alpha1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function. Mol Pharmacol. 2007, 71: 1015-1029. 10.1124/mol.106.033035.
Guo W, Shi L, Javitch JA: The fourth transmembrane segment forms the interface of the dopamine D2 receptor homodimer. J Biol Chem. 2003, 278: 4385-4388. 10.1074/jbc.C200679200.
Guo W, Shi L, Filizola M, Weinstein H, Javitch JA: Crosstalk in G protein-coupled receptors: changes at the transmembrane homodimer interface determine activation. Proc Natl Acad Sci USA. 2005, 102: 17495-17500. 10.1073/pnas.0508950102.
Guo W, Urizar E, Kralikova M, Mobarec JC, Shi L, Filizola M, Javitch JA: Dopamine D2 receptors form higher order oligomers at physiological expression levels. Embo J. 2008, 27: 2293-2304. 10.1038/emboj.2008.153.
Gonzalez-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV, Lopez-Gimenez JF, Zhou M, Okawa Y, Callado LF, Milligan G, Gingrich JA, Filizola M, Meana JJ, Sealfon SC: Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature. 2008, 452: 93-97. 10.1038/nature06612.
Gonzalez-Maeso J, Sealfon SC: Psychedelics and schizophrenia. Trends Neurosci. 2009, 32: 225-232. 10.1016/j.tins.2008.12.005.
Salahpour A, Angers S, Mercier JF, Lagace M, Marullo S, Bouvier M: Homodimerization of the beta2-adrenergic receptor as a prerequisite for cell surface targeting. J Biol Chem. 2004, 279: 33390-33397. 10.1074/jbc.M403363200.
Dorsch S, Klotz KN, Engelhardt S, Lohse MJ, Bunemann M: Analysis of receptor oligomerization by FRAP microscopy. Nat Methods. 2009, 6: 225-230. 10.1038/nmeth.1304.
Fonseca JM, Lambert NA: Instability of a class a G protein-coupled receptor oligomer interface. Mol Pharmacol. 2009, 75: 1296-1299. 10.1124/mol.108.053876.
Hern JA, Baig AH, Mashanov GI, Birdsall B, Corrie JE, Lazareno S, Molloy JE, Birdsall NJ: Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules. Proc Natl Acad Sci USA. 107: 2693-2698.
Gabilondo AM, Meana JJ, Barturen F, Sastre M, Garcia-Sevilla JA: mu-Opioid receptor and alpha 2-adrenoceptor agonist binding sites in the postmortem brain of heroin addicts. Psychopharmacology (Berl). 1994, 115: 135-140. 10.1007/BF02244763.
Gabilondo AM, Meana JJ, Garcia-Sevilla JA: Increased density of mu-opioid receptors in the postmortem brain of suicide victims. Brain Res. 1995, 682: 245-250. 10.1016/0006-8993(95)00333-L.
Gonzalez-Maeso J, Rodriguez-Puertas R, Meana JJ, Garcia-Sevilla JA, Guimon J: Neurotransmitter receptor-mediated activation of G-proteins in brains of suicide victims with mood disorders: selective supersensitivity of alpha(2A)-adrenoceptors. Mol Psychiatry. 2002, 7: 755-767. 10.1038/sj.mp.4001067.
Meana JJ, Gonzalez-Maeso J, Garcia-Sevilla JA, Guimon J: mu-opioid receptor and alpha2-adrenoceptor agonist stimulation of [35S]GTPgammaS binding to G-proteins in postmortem brains of opioid addicts. Mol Psychiatry. 2000, 5: 308-315. 10.1038/sj.mp.4000727.
Valdizan EM, Diez-Alarcia R, Gonzalez-Maeso J, Pilar-Cuellar F, Garcia-Sevilla JA, Meana JJ, Pazos A: alpha-Adrenoceptor functionality in postmortem frontal cortex of depressed suicide victims. Biol Psychiatry. 68: 869-872.
Jordan BA, Gomes I, Rios C, Filipovska J, Devi LA: Functional interactions between mu opioid and alpha 2A-adrenergic receptors. Mol Pharmacol. 2003, 64: 1317-1324. 10.1124/mol.64.6.1317.
Vilardaga JP, Nikolaev VO, Lorenz K, Ferrandon S, Zhuang Z, Lohse MJ: Conformational cross-talk between alpha2A-adrenergic and mu-opioid receptors controls cell signaling. Nat Chem Biol. 2008, 4: 126-131. 10.1038/nchembio.64.
Gonzalez-Maeso J, Weisstaub NV, Zhou M, Chan P, Ivic L, Ang R, Lira A, Bradley-Moore M, Ge Y, Zhou Q, Sealfon SC, Gingrich JA: Hallucinogens Recruit Specific Cortical 5-HT(2A) Receptor-Mediated Signaling Pathways to Affect Behavior. Neuron. 2007, 53: 439-452. 10.1016/j.neuron.2007.01.008.
Moreno JL, Holloway TD, Albizu L, Sealfon SC, Gonzalez-Maeso J: Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2Areceptor agonists. Neurosci Lett. 2011, 493 (3): 76-9. 10.1016/j.neulet.2011.01.046.
Rives ML, Vol C, Fukazawa Y, Tinel N, Trinquet E, Ayoub MA, Shigemoto R, Pin JP, Prezeau L: Crosstalk between GABAB and mGlu1a receptors reveals new insight into GPCR signal integration. Embo J. 2009, 28: 2195-2208. 10.1038/emboj.2009.177.
Magalhaes AC, Holmes KD, Dale LB, Comps-Agrar L, Lee D, Yadav PN, Drysdale L, Poulter MO, Roth BL, Pin JP, Anisman H, Ferguson SS: CRF receptor 1 regulates anxiety behavior via sensitization of 5-HT2 receptor signaling. Nat Neurosci. 13: 622-629.
Fung JJ, Deupi X, Pardo L, Yao XJ, Velez-Ruiz GA, Devree BT, Sunahara RK, Kobilka BK: Ligand-regulated oligomerization of beta(2)-adrenoceptors in a model lipid bilayer. Embo J. 2009, 28: 3315-3328. 10.1038/emboj.2009.267.
Dufau ML: The luteinizing hormone receptor. Annu Rev Physiol. 1998, 60: 461-496. 10.1146/annurev.physiol.60.1.461.
Rivero-Muller A, Chou YY, Ji I, Lajic S, Hanyaloglu AC, Jonas K, Rahman N, Ji TH, Huhtaniemi I: Rescue of defective G protein-coupled receptor function in vivo by intermolecular cooperation. Proc Natl Acad Sci USA. 107: 2319-2324.
Lee C, Ji I, Ryu K, Song Y, Conn PM, Ji TH: Two defective heterozygous luteinizing hormone receptors can rescue hormone action. J Biol Chem. 2002, 277: 15795-15800. 10.1074/jbc.M111818200.
Sangkuhl K, Schulz A, Schultz G, Schoneberg T: Structural requirements for mutational lutropin/choriogonadotropin receptor activation. J Biol Chem. 2002, 277: 47748-47755. 10.1074/jbc.M203491200.
Gupta A, Mulder J, Gomes I, Rozenfeld R, Bushlin I, Ong E, Lim M, Maillet E, Junek M, Cahill CM, Harkany T, Devi LA: Increased abundance of opioid receptor heteromers after chronic morphine administration. Sci Signal. 3: ra54-
Albizu L, Cottet M, Kralikova M, Stoev S, Seyer R, Brabet I, Roux T, Bazin H, Bourrier E, Lamarque L, Breton C, Rives ML, Newman A, Javitch J, Trinquet E, Manning M, Pin JP, Mouillac B, Durroux T: Time-resolved FRET between GPCR ligands reveals oligomers in native tissues. Nat Chem Biol. 6: 587-594.
Mouillac B, Manning M, Durroux T: Fluorescent agonists and antagonists for vasopressin/oxytocin G protein-coupled receptors: usefulness in ligand screening assays and receptor studies. Mini Rev Med Chem. 2008, 8: 996-1005. 10.2174/138955708785740607.
Ferre S, von Euler G, Johansson B, Fredholm BB, Fuxe K: Stimulation of high-affinity adenosine A2 receptors decreases the affinity of dopamine D2 receptors in rat striatal membranes. Proc Natl Acad Sci USA. 1991, 88: 7238-7241. 10.1073/pnas.88.16.7238.
Wu B, Chien EY, Mol CD, Fenalti G, Liu W, Katritch V, Abagyan R, Brooun A, Wells P, Bi FC, Hamel DJ, Kuhn P, Handel TM, Cherezov V, Stevens RC: Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science. 330: 1066-1071.
Palczewski K, Kumasaka T, Hori T, Behnke CA, Motoshima H, Fox BA, Le Trong I, Teller DC, Okada T, Stenkamp RE, Yamamoto M, Miyano M: Crystal structure of rhodopsin: A G protein-coupled receptor. Science. 2000, 289: 739-745. 10.1126/science.289.5480.739.
Rasmussen SG, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, Edwards PC, Burghammer M, Ratnala VR, Sanishvili R, Fischetti RF, Schertler GF, Weis WI, Kobilka BK: Crystal structure of the human beta2 adrenergic G-protein-coupled receptor. Nature. 2007, 450: 383-387. 10.1038/nature06325.
Rasmussen SG, Choi HJ, Fung JJ, Pardon E, Casarosa P, Chae PS, Devree BT, Rosenbaum DM, Thian FS, Kobilka TS, Schnapp A, Konetzki I, Sunahara RK, Gellman SH, Pautsch A, Steyaert J, Weis WI, Kobilka BK: Structure of a nanobody-stabilized active state of the beta(2) adrenoceptor. Nature. 469: 175-180.
Milligan G, Kostenis E: Heterotrimeric G-proteins: a short history. Br J Pharmacol. 2006, 147 (Suppl 1): S46-55.
Hu J, Wang Y, Zhang X, Lloyd JR, Li JH, Karpiak J, Costanzi S, Wess J: Structural basis of G protein-coupled receptor-G protein interactions. Nat Chem Biol. 6: 541-548.
Oldham WM, Hamm HE: Heterotrimeric G protein activation by G-protein-coupled receptors. Nat Rev Mol Cell Biol. 2008, 9: 60-71. 10.1038/nrm2299.
Han Y, Moreira IS, Urizar E, Weinstein H, Javitch JA: Allosteric communication between protomers of dopamine class A GPCR dimers modulates activation. Nat Chem Biol. 2009, 5: 688-695. 10.1038/nchembio.199.
Bayburt TH, Leitz AJ, Xie G, Oprian DD, Sligar SG: Transducin activation by nanoscale lipid bilayers containing one and two rhodopsins. J Biol Chem. 2007, 282: 14875-14881. 10.1074/jbc.M701433200.
Whorton MR, Bokoch MP, Rasmussen SG, Huang B, Zare RN, Kobilka B, Sunahara RK: A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc Natl Acad Sci USA. 2007, 104: 7682-7687. 10.1073/pnas.0611448104.
Vilardaga JP, Bunemann M, Krasel C, Castro M, Lohse MJ: Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. Nat Biotechnol. 2003, 21: 807-812. 10.1038/nbt838.
Monnier C, Tu H, Bourrier E, Vol C, Lamarque L, Trinquet E, Pin JP, Rondard P: Trans-activation between 7TM domains: implication in heterodimeric GABAB receptor activation. Embo J. 30: 32-42.
One of a series of four reviews on G protein-coupled receptors published in memory of Hubert H. M. Van Tol (1959-2006), formerly Head of Molecular Biology at the Centre for Addiction and Mental Health, and a Professor in the Departments of Psychiatry and Pharmacology at the University of Toronto. Hubert's contributions to G protein-coupled receptor research and neuroscience are numerous and are best remembered by his central role in the cloning of the dopamine receptor family. His many achievements were recognized through awards such as the John Dewan award, The Prix Galien, and the Joey & Toby Tanenbaum Distinguished Scientist Award for Schizophrenia Research.
This work was supported by the National Institutes of Health grant 5R01MH084894.
The authors declare that they have no competing interests.
JGM searched the literature, conceived the topic of the review and wrote the manuscript
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.