measurement in dual patch recording. (a) Voltage (V) and current (C) modes were alternated between E1 and E2 to obtain two sets of RM and Ra values for comparison. (b) Open black and red circles are RM1-RM2 and Ra1-Ra2 values, respectively (n = 44). Despite a large variation in Ra1-Ra2, the RM1-RM2 varies minimally in the same cells. (c) The RM1 (black, from E1) and RM2 (red, from E2) values were sorted into 2 MΩ intervals and plotted against their respective occurrence frequencies; the frequency distribution patterns are nearly identical for the data obtained from E1 and E2 (n = 54). (d) The pooled Ra and RM values are not correlated. (e) Variation of Ra between the two electrodes does not affect the accuracy of RM measurement. (f) The long-term RM recording remains stable and is independent of variation in Ra. (g) RM1-RM2 varies minimally in dual patch recording compared to measurements obtained from the “membrane test” (pClamp 9.0 software). (h) Within the acceptable range of resting potentials (Vr), VM does not affect the accuracy of RM measurement.