Dual patch voltage clamp study of low membrane resistance astrocytes in situ
© Ma et al.; licensee BioMed Central Ltd. 2014
Received: 27 January 2014
Accepted: 13 March 2014
Published: 17 March 2014
Whole-cell patch clamp recording has been successfully used in identifying the voltage-dependent gating and conductance properties of ion channels in a variety of cells. However, this powerful technique is of limited value in studying low membrane resistance cells, such as astrocytes in situ, because of the inability to control or accurately measure the real amplitude of command voltages. To facilitate the study of ionic conductances of astrocytes, we have developed a dual patch recording method which permits membrane current and membrane potential to be simultaneously recorded from astrocytes in spite of their extraordinarily low membrane resistance. The utility of this technique is demonstrated by measuring the voltage-dependent activation of the inwardly rectifying K+ current abundantly expressed in astrocytes and multiple ionic events associated with astrocytic GABAA receptor activation. This protocol can be performed routinely in the study of astrocytes. This method will be valuable for identifying and characterizing the individual ion channels that orchestrate the electrical activity of low membrane resistance cells.
KeywordsAstrocytes Voltage clamp Membrane resistance Kir4.1 GABAA receptor
When patch clamp was first applied to study glial cells in brain slices in the early 1990s, it became immediately clear that in a subpopulation of glial cells, characterized by a linear current-to-voltage (I-V) relationship passive K+ membrane conductance and low membrane resistance (RM), adequate voltage clamp quality could not be achieved [1, 2]. In later studies, this subpopulation of glial cells has been unequivocally shown as the only electrophysiological phenotype of astrocytes in rat hippocampus when animals reach adulthood [3, 4]. Therefore, the expression of passive membrane K+ conductance is a characteristic of functional mature astrocytes. In view of a widespread distribution of passive astrocytes in various brain regions and in species ranging from low amphibians to humans, it is possible that the distinctive passive K+ conductance and low RM are common features of mature astrocytes in the brain.
Despite of an increasing awareness of the functional importance of astrocytes in the brain, the exceptionally low RM, estimated in the range of 2–5 MΩ, creates a formidable barrier for patch clamp voltage study of this major glial type. Specifically, the low RM introduces a large error because a significant fraction of the voltage drop during a command potential occurs across the electrode tip rather than the cell membrane . Because of this limitation, it has been difficult to identify and characterize the ion channels and transporter activities that establish the electrical properties of astrocytes. In particular, astrocytes are known to express a variety of K+ channels, including inwardly rectifying Kir4.1 and two-pore domain (K2P) TWIK-1 and TREK-1 leak-type K+ channels. The inability to effectively voltage clamp astrocytes has prevented these currents from being individually characterized with respect to their relative functional expression, conductance, rectification, contribution to VM, and modulation by neurotransmitters through activation of G-protein-coupled receptors. Therefore, an alternative electrophysiological method is urgently needed, and if such a new method could be successfully developed, voltage clamp study would be invaluable to gain insights into the basic electrophysiological properties of astrocytes in the adult brain.
Rationale and validation of dual patch single cell recording technique
To circumvent the limitation imposed by conventional whole-cell patch clamp recording for study of low RM astrocytes, we have developed a dual patch voltage clamp method. In this method, two patch electrodes are sealed to the cell body of a single astrocyte in the whole-cell configuration. This design has been conceived to overcome the following limitations inherited by single electrode voltage patch clamp for study of low RM cells. In single electrode patch clamp recording, the RM is typically calculated from the fit of a voltage command (VC) induced membrane current (I) near the resting membrane potential that carries a large error in low membrane resistance cells (see details in the following sections). In contrast, in dual patch recording, the VC is applied through one patch electrode, while a second electrode is used to measure the VC-induced actual change in membrane potential (VM). Using this method, an accurate current-to-voltage (I-VM) relationship can be obtained over a wide range of potentials, and the voltage-dependent membrane conductance can be reliably calculated. An additional unique opportunity offered by dual patch recording is that the I, VM, and RM can be measured and calculated simultaneously. This, however, needs to be experimentally validated.
In the present study, we started with a direct measurement of voltage errors in low RM astrocytes with dual patch recording. That was followed by the validation of dual patch recording for reliable and accurate measurement of RM. Building upon these, we further showed that the voltage dependent activation kinetics of inwardly rectifying K+ channel conductance can be established and pharmacologically studied. Finally, astrocytic ionotropic GABAA receptor was used as an example to show the simultaneous measurement of I, VM, and RM and disclosure of multiple ionic events associated with astrocytic GABAA receptor activation.
A lack of voltage control in astrocyte recording in situ
Since the total membrane resistance (Rt) is the sum of access resistance (Ra) and membrane resistance (RM) in series , to confirm that the dual patch results obey the basic voltage-division principle as in single electrode patch clamp recording, we used ΔVM/ΔVC as an indicator of voltage clamp quality that theoretically follows a relationship of ΔVM/ΔVC = RMRt-1. We plotted Rt against ΔVM/ΔVC and fit the data with ΔVM/ΔVC = aRt b from 44 dual patch recordings (Figure 1g). Of note, to obtain an accurate fit, the Rt greater than 30 MΩ that are routinely discarded in study were included in this analysis. As shown in the Figure 1g, the fit yielded a RM (a) of 5.73 MΩ that was almost identical to the measured RM (see next section). Importantly, the fit yielded an exponent (b) of -0.995, which matches closely to a theoretically ideal value of -1, indicating that the results derived from the two electrodes in dual patch recording follow accurately to the voltage-division principle.
In dual patch recording, E1 and E2 are separated by 3.4 ± 0.2 μm (n = 15), and the VM measured from E2 (VM,2) is used to represent the VM in E1(VM,1) to establish the actual I-VM relationship, therefore to what extent does VM,2 reflect the intended VM,1 is an issue to be resolved for the concern of space clamp error, especially for low RM astrocytes. To estimate VM,1 in E1, we assumed that Ra is 1.5-fold of the recording electrode resistance (Rp) and used a -40 mV V c to induce current (I). Subsequently, the VM,1 is calculated from (V c-1.5 Rp*I)/I, and the fidelity of VM,2, in the form of VM,2/VM,1, is estimated at 93.9% (n = 4). Thus, within a tip-to-tip distance < than 4 μm in our study, VM,2 can be used with high fidelity for I-VM plot and for the RM measurement that will be described in the following section.
RM can be accurately measured in dual patch single cell recording
The low RM of passive astrocytes is the cause of poor voltage clamp quality. In dual patch recording, the VC-induced I and the actual voltage drop across the membrane VM, are measured separately by the two electrodes, so that RM can be calculated accurately from RM = VM/I. Using dual patch recording, the voltage and current clamp modes can be alternated between E1 and E2 (Figure 2a), allowing RM and Ra to be measured for each electrode from the same cell. We therefore analyzed these results to determine 1) whether RM1 and RM2 are identical in the same cell, as expected, and 2) whether the RM in dual patch recording is truly independent of the variation of Ra. We used the difference in measured access resistances (Ra1-Ra2) and membrane resistances (RM1-RM2) to determine the variation of these two parameters in the same cell. The results showed that, in the same cell, the Ra varied considerably between E1 and E2 (Figure 2b, red open circles), while the RM1 and RM2 were essentially identical (Figure 2b, black open circles). We next compared the measured RM values from E1 and E2 and found that these were nearly identical (RM1, 6.00 ± 0.32 MΩ vs. RM2, 6.12 ± 0.33 MΩ, n = 54, P = 0.79). Finally, we sorted RM1 and RM2 data sets separately with 2 MΩ intervals and plotted them against their respective occurrence frequencies to determine the pattern of frequency distributions. As shown in Figure 2c, the frequency distribution of the data sets between RM1s and RM2s matched almost perfectly (n = 54), which is consistent with the view that RM can be reliably measured in dual patch recording.
Theoretically, RM measurement is independent of Ra variation in dual patch recording. This was confirmed from the following experiments. First, in the correlation analysis shown in Figure 2d, RM is not correlated with Ra. Second, the Ra1 and Ra2 measured from the same cell typically differ from each other, we next sorted out the large Ra (Ra,L) and small Ra (Ra,S) data sets into two groups from 10 dual patch recordings (Figure 2e). As anticipated, the Ra,L and Ra,S varied significantly (Ra,L, 23.02 ± 6.07 MΩ vs. Ra,S, 13.6 ± 2.8 MΩ, n = 10, P = 0.034), but their corresponding RM,L (4.85 ± 0.74 MΩ) and RM,S (4.90 ± 0.77 MΩ) were almost identical (n = 10, P = 0.74). Third, while the Ra varies considerably during long-term recording, the simultaneously measured RM remained constant (Figure 2f).
In pClamp software, RM can be measured from the “Membrane test” program, which has been widely used for study of astrocyte electrophysiology [5, 8]. We found a much large variation between RM1 and RM2 data sets obtained from “Membrane test” than the data set from dual patch recording (Figure 2g), indicating that dual patch offers a more reliable and precise RM measurement.
It should be noted that in single electrode recording, Ra is resolved from time constant (τ), i.e., τ= RaCM. The accuracy of Ra estimation depends on a close approximation of Ra to the effective access resistance (Ra,eff); that is Ra,eff = RaRM/(Ra + RM) . In high RM cells, such as neurons, RM > > Ra, so that Ra,eff ≅ Ra, and Ra can be resolved with good confidence. However, in low RM astrocytes, because Ra,eff ≅ RM, the Ra measured from “Membrane test” (pClamp9.0) is erroneous, so is RM. The latter is calculated from RM = Rtotal-Ra. Although we know that RM in astrocyte is extremely low, but reliable RM measurement is not yet available for the noted reason. Accordingly, our report provides a yet most reliable RM measurement from mature astrocytes.
Within the acceptable whole-cell resting membrane potentials (Vr), i.e., more negative than -75 mV , the Vr values always vary slightly between E1 and E2 in the same cell. To ensure that within the accepted Vr range, variation of Vr does not affect RM measurement, we sorted all the positive Vr (Vr,p) and negative Vr (Vr,n) from 19 dual patch recordings into two separate groups for RM comparison. While the Vr,p (-77.97 ± 0.50 mV) and the Vr,n (-79.81 ± 0.45 mV) varied significantly (n = 19, P = 8.91 × 10-11), the RM was essentially the same between the two groups (RM,p, 6.10 ± 0.54 MΩ vs. RM,n, 6.13 ± 0.53 MΩ, n = 19, P = 0.83) (Figure 2h).
The experiments described above used young adult mice of 3–4 weeks old. To determine if this protocol is also applicable for astrocytes from fully mature mice brain, we next prepared slices from 5-month old mice. We found that dual patch single astrocyte recording remains feasible. At this animal age, dual patch recording revealed a similar Vr of -78.75 ± 0.47 mV (n = 7), and a RM of 5.91 ± 1.52 MΩ (n = 5) compared to young adult mice.
In summary, we show that in dual patch recording, the RM can be measured accurately and reliably, and the majority of astrocytes exhibit RM values in the range of 4–8 MΩ. Importantly, since astrocytes are non-excitable, most of their ionic events can be monitored directly by RM measurement. Thus, the ability of dual patch to permit long-term stable RM measurement should be highly useful for pharmacological analysis of functional proteins in astrocytes, such as K+ channels and transporters.
The temporal resolution in astrocyte dual patch recording
Theoretically, a higher temporal resolution in astrocytes should also be attributable to the low RM. Because τ is the product of effective access resistance (Ra,eff) times CM, where Ra,eff = Ra.RM/(Ra + RM) . As noted above, the Ra,eff approximates to RM in low RM astrocytes, which results in a faster τ compared to high RM neurons.
Because of an extremely high leak K+ conductance, it is worth noting that the likelihood to observe a voltage dependent conductance in astrocyte whole-cell current depends chiefly on the expression quantity of the candidate channel, but not the temporal resolution of dual patch recording.
The advantage of using a combination of MultiClamp 700B (or 700A) amplifier and pClamp 9 (or 10) software is that the voltage and current clamp mode can be designated either the same or differently between E1 and E2. As the illustration shown in Figure 2a, E1 and E2 can be initially programed in voltage (V) and current (C) clamp mode, respectively, and then swapped in the following experiment. For the electrode in V mode, the controlled input is V C and the recorded signal is I. For the electrode in C mode with no holding current, the recorded signal is the V M that is induced by V C from another electrode in the pair. The voltage dependence and activation kinetics of ion channels of interest can then be constructed by plotting the measured I values against their corresponding V M values (Figure 2a).
The measured V M from E2 can be much smaller than that of the applied V C in E1, which could make accurate V M calculation difficult. When V M cannot be detected with confidence, increase V C to ensure a reliable V M reading. Likewise, it is beneficial to set up one of the electrodes in the pair with a relatively small R t in V mode.
The high leak K+ conductance is the cause of difficulty for voltage clamp identification and pharmacological study of a specific K+ channel of interest. The following examples will demonstrate how a combined pharmacology and current subtraction strategy can be used to overcome this obstacle in dual patch recording.
Channel inhibitors or activators are not yet available for most of two-pore channels, use of genetically modified mice, such as TWIK-1 and TREK-1 knockout mice, should be highly valuable to gain insights into the molecular identify and function of astrocyte passive conductance in the future .
The procedure described here should be equally useful for study of other cell type with similarly low R M. When identification of ion channel with fast activation/inactivation kinetics is the purpose of study, a low R a in both electrodes is essential and the commonly used conventions for single electrode recording also apply to dual patch recording.
When set up E1 and E2 in V and C mode, respectively, E2 only observes the deviation of V M from the intended V C. The measured V M from E2 is not compensated for either dynamic or steady-state voltage errors. The former error is associated with the onset of a V C and is proportional to the time constant (τ=R a C M). The latter refers to the deviation of V M from the steady-state holding V C in response to rapidly activated ionic conductances, such as ionotropic GABAA receptor in astrocytes (Figure 4). As noted above, effective R a compensation cannot be reliably performed without the guidance of E2 as voltage monitor. Also, astrocytes show a faster V M onset temporal resolution and unlikely express large amount of ion channels with rapid activation kinetics. Therefore, unless an experiment is designed to target on a specific voltage gated ionic conductance, R a compensation is not recommended.
Other electrophysiological methods, such as discontinuous single electrode voltage clamp (dSEVC) and two electrode voltage clamp (TEVC) technique, typically use sharp electrode, which is technically unfeasible for mammalian astrocytes with a some diameter less than 10 μm. Additionally, no validated and commercially available amplifiers are yet available for the intended goal of present research. Therefore, at least in the current stage, these techniques cannot be considered as alternatives.
Measurement of voltage-dependent membrane conductance: inwardly rectifying K+ channels
In dual patch RM measurement, one minute 100 μM Ba2+ bath application increased RM from the control 7.46 ± 1.2 MΩ to 13.7 ± 3.1 MΩ (n = 7), corresponding to a membrane conductance (GM) decrease from 0.134 μS in the control to 0.073 μS in the Ba2+. Based on this analysis, Kir4.1 accounts for ~45.5% of the GM (Figure 4c).
As the VC-induced actual VM can be reliably determined to construct the actual I-VM relationship for a conductance of interest, we next adjusted the VC to reach the intended VM from -60 mV to -100 mV, where the inward rectification kinetics of Kir4.1 should be evident . The I-VM curves shown in Figure 4d revealed a selective inhibition of inward whole-cell currents by Ba2+. To analyze the results statistically, we used voltage dependent GM to describe the rectification characteristics. We show that a voltage-dependent inward GM was clearly evident in the control condition (Figure 4e-f), which is consistent with the presence of functional Kir4.1 currents. Furthermore, this inwardly rectifying conductance was totally inhibited by 100 μM Ba2+. These results demonstrate that dual patch allows reliable establishment of I-VM relationship and, in the present case, enables the functional identification and pharmacological analysis of inwardly rectifying Kir4.1 channels in astrocytes.
Identification of multiple ionic events associated with GABAA receptor activation
A unique technical advantage of dual patch recording is that I, VM and RM can be measured and resolved simultaneously. We chose the astrocytic GABAA receptor as an example to experimentally demonstrate the feasibility of this powerful application.
We have recently shown that, while GABAA activation decreases Rt in both neurons and NG2 glia, GABAA activation increases Rt in astrocytes, which is likely caused by a rapid secondary K+ channel inhibition . However, single electrode recording in either voltage or current clamp mode does not provide a temporal resolution to separate these ionic events in astrocytes. Therefore, we further explored this issue in dual patch recording, where GABA-induced changes in VM, I and RM can be obtained simultaneously. Interestingly, astrocytes at different developmental stages responded to GABA differently. At P13, immature astrocytes responded to 1 mM GABA application by VM depolarization, activation of inward currents and RM decrease (5.96 ± 1.26 MΩ in control vs. 3.37 ± 0.82 MΩ in GABA, n = 5, P = 0.026) (Figure 5a-b), responses which were identical to neuronal GABAA receptor activation . By comparison, in mature P21 astrocytes, while 1 mM GABA also induced inward currents and VM depolarization, three obvious ionic events could be resolved in RM measurements (Figure 5c-d). Specifically, the RM first increased from the control 5.27 ± 0.56 MΩ to 5.77 ± 0.63 MΩ (n = 6, P = 0.0068), then decreased significantly (4.97 ± 0.57 MΩ, n = 6, P = 0.015). This effect was followed by a second sustained RM increase, implying a complex modulation of astrocyte membrane conductance upon GABAA activation.
As noted above, simultaneous measurement of I, VM and RM is feasible in dual patch recording. Additionally, only in this recording mode, multiple ionic events associated with astrocytic ionotropic GABAA receptor activation could be readily identified. This powerful application should also be applicable for revealing complex ionic events in other cell types, such as neurons.
Summary of technical advantage of dual patch single astrocyte recording
As noted before, the R a and R M measurement from single electrode “Membrane test” software program are erroneous; this protocol allows accurate, stable and long-term R M measurement from astrocytes. For non-excitable cells, this should have a broad usage for reliable pharmacological analysis of ion channel and electrogenic transporter activity in astrocytes.
An important purpose of this protocol is to validate whether the voltage dependence of a specific ionic conductance can be established and analyzed. We show from the G M analysis of Kir4.1 currents that this is feasible (Figure 4).
A unique advantage we demonstrated in this protocol is that membrane I, V M, and R M can be measured simultaneously in dual patch recording model. As shown in the example of astrocytic GABAA receptor study, multiple ionic events can be disclosed following to GABAA receptor activation (Figure 5).
Although it may not be highly useful, using E2 as a monitor, it is possible to achieve a nearly perfect voltage clamp quality with R a compensation function (Figure 1h). This can not be done in single electrode voltage clamp recording.
The voltage clamp method is designed to reveal the voltage dependence of membrane conductances. Using Kir4.1 channel currents as an example (Figure 4), we demonstrated that the voltage dependence can be accurately established and pharmacologically analyzed in dual patch recording. However, even though the temporal resolution of VM onset is faster in astrocytes (Figure 3), identification of low density expressing voltage-dependent ion channel conductance in low RM astrocytes remains challenging. For example, immature astrocytes express voltage- and time-dependent outward transient K+ channels , but whether the expression of these channels continues in mature astrocytes cannot be answered with confidence with dual patch recording. Nevertheless, there is no evidence indicating extensive expression of voltage gated ion channels in astrocytes .
It is worth noting that two recent reports have successfully used dual patch single astrocyte recording to correct voltage error for analyzing the relative glutamate vs. Cl- permeability ratio through Best1 channel , and identifying pH sensitive and anesthetic-sensitive leak K conductances in hippocampal astrocytes . Thus, the dual patch method validated in this study provides a powerful means for studying the voltage dependence of leak type K+ channels, slow cycling transporters, and pumps that are abundantly expressed in astrocytes.
CaCl2, glucose, HEPES, KCl, KOH, Mg-ATP, MgCl2, Na2-GTP, NaCl, NaHCO3, NaH2PO4 (all from Sigma-Aldrich, St. Louis, MO)
Sulforhodamine 101 (SR101) from Invitrogen (New York, NY)
Deionized water with 18.2 MΩcm at 25°C ▲CRITICAL The quality of water is especially important for internal solutions.
C57BL/6 J mice from postnatal day (P) 9–30, and 5 months old were used in different experiments noted. ! CAUTION All animal studies must be approved by the Institutional Animal Care and Use Committee. All the relevant ethics regulations have to be strictly followed. ▲CRITICAL The animal age needs to be carefully chosen depending on experimental purpose and we chose to use animals older than postnatal day 21 in most of the experiments as hippocampal astrocytes become mature after this postnatal day. To validate this protocol for study astrocytes from fully mature mice, 5-month old mice were used in some of experiments.
An electrophysiology setup for submerged slices equipped with ×4 and ×40 infrared differential interference contrast (IR-DIC) visualization, a patch-clamp amplifier capable of current and voltage clamp and providing two channel recording (e.g., MultiClamp 700A or 700B, Molecular Devices), an interface for converting digital-analog signals between the amplifier and a computer (e.g., Digidata 1322A, Molecular Devices).
(Optional) A fluorescent imaging system (e.g., Polychrome V system from Till Photonics, Germany) is advantageous for high resolution visualization of small glial soma and placing of dual patch electrodes on it. This system can also be used for identifying astrocyte based on SR101 positive staining. This system can also be used for simultaneous dual patch and ion sensitive dye measurement.
Two micromanipulators for automatic loading of two electrodes to the top of the brain slice can shorten the loading time (e.g., PatchStar from Scientifica, UK).
Commercially available borosilicate glass capillaries, such as the one from Warner Instruments (Cat. no. 64–0772), worked well in this experiment. An open electrode resistance in the range of 2.5-5 MΩ, when filled with KCl-based electrode solution, is well suited for dual patch recordings. However, for low RM astrocytes, electrode resistance in the low-end of this range is recommended.
Brain slice cutting solution (in mM): 125 NaCl, 3.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 0.1 CaCl2, 3 MgCl2, and 10 glucose. Cutting solution should be freshly prepared on the day of the experiment.
Artificial cerebral spinal fluid (aCSF) (in mM): 125 NaCl, 3.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 2 CaCl2, 1 MgCl2, and 10 glucose (osmolality, 295 ± 5 mOsm) at room temperature (20–22°C).
Electrode solution (in mM): 140 KCl, 0.5 CaCl2, 1 MgCl2, 5 EDTA, 10 HEPES, 3 Mg2+-ATP, and 0.3 2Na+-GTP. This solution was titrated with KOH to pH 7.25-7.27, and the final osmolality was 280.0 ± 5.0 mOsm. The solution can be prepared in advance and stored at -80°C in ~0.5 ml aliquots.
SR101: Stock solution, 6 mM; working solution, 0.6 μM.
1 | Use a commercially available Vibratome, e.g., Pelco 1500, to prepare 250 μm thickness coronal hippocampal slices from mouse brain with a standard procedure we and others described before [5, 16]. All solutions should be continuously bubbled with 95%O2/5%CO2.
2 | Place acute slices in a nylon net basket slice holder immersed in aCSF chamber for recovery and storage. The aCSF is continuously bubbled with 95%O2/5%CO2 for a typical experiment day of 6 to 8 hrs.
3 | For SR101 staining, transfer slice containing basket to another aCSF chamber containing 0.6 μM SR101 at 34°C for 30 min. Then, transfer the same basket back to the normal aCSF at room temperature for at least 1 h before experiment.
4 | For recording, transfer one brain slice to the recording chamber, lay it down on the bottom of glass chamber and gently place a platinum slice anchor (Warner Instruments) on the top of slice to prevent movement of slice from bath perfusion. Perfuse oxygenated aCSF at a rate of 2.5 ml/min.
5 | Fill two patch electrodes with electrode solution and install them to the electrode holders. Apply a small and constant positive pressure to the internal solution. Use IR-DIC with ×4 objective to identify the surface of the slice. Move the tips of electrodes to the region of interest, i.e., CA1 stratum radiatum. In voltage clamp mode, apply a recurrent 5 mV square voltage pulse to monitor the electrode resistance. Switch to the water immersion ×40 objective. Apply a drop of bath solution to the objective that runs down and forms a fluid bridge between the front lens and the slice. Advance electrodes into the slice ~ 50 μm beneath the surface.
6 | Select a viable glial cell based on an irregular soma shape with a diameter around 10 μm. Typically, one or more primary processes stemming from the soma are visible (Figure 1a). To confirm an astrocytic identify, SR101 staining can be used. However, be aware that an adverse effect on neuronal excitability has been reported with SR101 staining . After establishing the whole-cell configuration, mature astrocytes can be unequivocally identified by the expression of passive conductance (Figure 1b). Alternatively, astrocytes can be identified solely and easily by SR101 staining. Switch to IR-DIC visualization.
7 | Approach the cell slowly with one electrode at a time. Once on-cell, see dimpling and release the electrode pressure. Apply negative potential (ranging from -70 to -80 mV) through the commander panel to facilitate seal formation. This manipulation typically results in the rapid formation of a GΩ seal. Repeat this procedure with the second electrode. Check and keep the position of first electrode relative to the cell. Finally, ensure both electrodes to form GΩ seal on the same cell.
▲CRITICAL This is the most critical step requiring careful coordination of two electrodes and readjustment of the relative position between cell soma and electrodes. Often, when the targeted cell is approached by the second electrode, the cell can move away from the first electrode. Adjust the first electrode to follow the cell when it is necessary.
8 | Rupture the patch of membrane isolated inside the electrode tip by negative pressure pulses to establish the whole-cell configurations. No obvious change in holding current shift associated with a decrease of seal test resistance to < 30 MΩ is an indication of successful formation of whole-cell configuration. Repeat this for the second electrode. After both whole-cell configurations are established, wait at least 5 min to allow ion equilibration between electrode solution and intracellular cytoplasm
To evaluate the voltage dependence of channel activation, apply a series of command voltage steps, e.g., -210 mV to +30 mV in 40 mV increments, from the holding potential of -80 mV
To monitor change of R M (or R t) in response to drugs, e.g., GABA, apply a single negative command voltage step, e.g., -60 mV from holding potential for 20 ms.
I-V M relationship can be derived from the stepwise command voltages as shown in Figure 2a. Plot I readings from E1 against their corresponding V M readings from electrode 2. Determine the reversal potential of the current by extrapolating the data point on the I-V M curve.
Plot the conductance-voltage (G-V M) curve by dividing the current amplitude change (ΔI) by the measured voltage step (ΔV M) at each potential. As shown in Figure 4f.
Brain slice quality is always the first thing to be considered. Ensure the preparation procedure was followed strictly; otherwise a new preparation is needed.
The difficulty in achieving GΩ seal increases with animal age, start your practice with P10 mice is recommended to familiarize the procedure. Young adult mice from P21 - P30 can be used in most of experiments, unless the study targets on an age related subject.
Make sure to apply and maintain a small positive pressure before the electrodes touching to the bath solution in step 5.
Apply the suction as gentle as possible and increase it in a stepwise manner until the membrane is ruptured.
Ensure electrode solution is freshly prepared with correct pH and osmolality.
Focus on the cell when the second electrode approaches the cell. Move the first electrode to follow the cell if the cell moves.
Adjust slice anchor and hold more tightly if too loose.
The deeper the targeted cell beneath the surface of slice, the more likely to encounter a cell movement during the handling the second electrode to approach the cell. Select a relatively shallow cell, ~50 μm is recommended.
Approach the cell gently. Avoid larger movements of the second electrode, including approaching, going up or down. If the tip of the second electrode is too shallower or deeper than cell body, withdraw the second electrode gently, adjust the position on the top of slice and re-approach.
In our experience, a relatively strong and fast pulse works well to rupture membrane and achieve a relatively stable R a .
Keep the same or increase the suction pressures in the repetitive rupture attempt, release pressure immediately if membrane resistance in seal test drops suddenly.
Keep a small pressure and click “Zap”, and release the pressure after Zap. Repeat Zap if necessary.
If the seal resistance is higher than 30 MΩ, indication of R t >30 MΩ, repeat above steps may decrease the R t of whole-cell recording.
Low resistance electrodes, i.e., 2.0-2.5 MΩ, has been demonstrated to be feasible and is recommended ; as such, the R t can be physically reduced to the lowest workable range.
Empirically, a relatively stronger suction pressure for whole-cell break-through results in a high percentage of low and stable R a whole-cell recordings.
To ensure that the brain slice is anchored securely in the chamber; this prevents potential slice movement resulting from perfusion pulsatile.
In general, the older the animal the more difficulty to achieve a long-lasting stable R a, use young adult animals unless the experiment examining an ageing related question.
Use mechanically stable micromanipulators.
We routinely discard recordings with high R a (or R t) from data analysis.
Step 1, slice preparation: 30 min
Step 2–3, slice recovery and SR101 staining: at least 1 h
Step 4, transfer slice to recording chamber and re-equilibration: 20 min
Step 5, loading both electrodes: 10 min
Step 6, select astrocyte: 5 min
Step 7, approach the cell and form GΩ seals: 5–30 min
Step 8, break through to form dual patch recording: 3–10 min
Step 9, recording: 30 min - 4 h, depending on the experimental design
Step 10, Analysis: variable, depending on required data.
Dual patch recording was once considered to be a time-consuming technique which required considerable skill. Facilitated by the use of advanced equipment, specifically PatchStar micromanipulators and software (Scientifica, UK), a Polychrome V imaging system (Till Photonics, Germany), and a MultiClamp 700A/700B amplifier (Molecular Devices, Sunnyvale, CA), this recording mode can be performed routinely, studying 3–8 cells in a 6-hour experimental day. For experienced researcher, the dual patch single astrocyte recording can be achieved within 10 min after the cell being visual identified. Should all the steps be followed carefully, successful dual patch single astrocyte recording can be readily achieved. In some of the experiments, successful dual patch recordings recording had lasted up to 4 hours in our designed experiment. Thus, dual patch is an ideal technique for the future study of functional channels, receptors, and electrogenic transporters in astrocytes.
This work was sponsored by grants from the National Institute of Neurological Disorders and Stroke (RO1NS062784 to MZ) and the National Natural Science Foundation of China (81000491to GX). We thank Ms. Judith A. Enyeart and Ms. Kelly E. Crowe for their assistance in manuscript preparation, Mr. Randall Carpenter for critical reading of manuscript.
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