The expression of Pet1 and Gata3 , but not LMX1B was decreased in the dorsal raphe nuclei of Tph2-/-mice. (A and B) Expression of Pet1 (A) and Gata3 (B) detected by in situ hybridization in the dorsal and caudal raphe nuclei of Tph2+/+ mice and Tph2-/- mice at embryonic day 17.5 (E17.5), postnatal day 0 (P0) and adult stages. (C) Immunohistochemistry images and quantified data to show LMX1B expression in the dorsal and caudal raphe nuclei of adult Tph2+/+ mice and Tph2-/- mice. Error bars represent SEM. n = 6. *P < 0.05, versus Tph2+/+, unpaired t test. Scale bars, 100 μm.