Fig. 8
From: Glycine confers neuroprotection through microRNA-301a/PTEN signaling
miR-301a mediates glycine-induced neuroprotection through suppression of PTEN. a, Transfection of PTEN cDNA reduces glycine-induced neuroprotection in cultured cortical neurons subjected to OGD insult. Left, LDH assay; Right, MTT assay (n = 6, *P < 0.05 vs. Sham, # P < 0.05 vs. Inj, **P < 0.05 vs. Inj + Gly, ANOVA test). b, Suppression of PTEN by PTEN siRNA in cultured cortical neurons does not increase glycine-induced neuroprotective effect in OGD insult (n = 6, *P < 0.05 vs. Sham, # P < 0.05 vs. Inj, ANOVA test). Left, LDH assay; Right, MTT assay (n = 6, *P < 0.05 vs. Sham, # P < 0.05 vs. Inj, ANOVA test). c, PTEN siRNA treatment prevents miR-301a antagomir from blocking glycine-induced neuroprotection. Left, LDH assay; Right, MTT assay (n = 6, *P < 0.05 vs. Sham, # P < 0.05 vs. Inj, **P < 0.05 vs. Inj + Gly, ## P < 0.05 vs. Ant + Inj + Gly, ANOVA test). Inj, injury; Gly, glycine; Ant, antagomir; Ant-con, antagomir control; Ago, agomir; Ago-con, agomir control; PT-cDNA, PTEN cDNA; PT-siR, PTEN siRNA