- Open Access
Glycine confers neuroprotection through microRNA-301a/PTEN signaling
© The Author(s). 2016
- Received: 14 April 2016
- Accepted: 17 May 2016
- Published: 26 May 2016
Glycine is known to protect against neuronal death. However, the underlying mechanism remains to be elucidated. The microRNA-301a is involved in both biological and pathological processes. But it is not known whether microRNA-301a has a neuroprotective property. In this study, we aimed to determine whether glycine-induced neuroprotection requires microRNA-301a-dependent signaling.
We provided the first evidence that glycine increased the expression of microRNA-301a in cultured rat cortical neurons and protected against cortical neuronal death through up-regulation of microRNA-301a after oxygen-glucose deprivation. MicroRNA-301a directly bound the predicted 3′UTR target sites of PTEN and reduced PTEN expression in cortical neurons. We revealed that PTEN down-regulation by microRNA-301a mediated glycine-induced neuroprotective effect following oxygen-glucose deprivation.
Our results suggest that 1) microRNA-301a is neuroprotective in oxygen-glucose deprivation-induced neuronal injury; 2) glycine is an upstream regulator of microRNA-301a; 3) glycine confers neuroprotection through microRNA-301a/PTEN signal pathway.
Glycine is the simplest non-essential amino acid, which is a critical building block in many proteins. Glycine is essential for the synthesis of many biomolecules such as creatine, porphyrins and purine nucleotides. Glycine plays a fundamental role in cell metabolism [1, 2]. In the adult CNS, glycine is a major inhibitory neurotransmitter that binds to glycine receptor (a chloride-permeable ion channel) to inhibit postsynaptic neurons [3–7]. Interestingly, glycine is also a co-agonist of the excitatory NMDA receptor, a calcium-permeable ion channel in the CNS [8, 9]. Glycine has been shown to have neuroprotective effect in a variety of experimental models including ischemia–reperfusion injury, anoxia, hypoxia, ROS, chemically induced energy depletion [10–13]. In double-blinded, placebo-controlled clinical trial, glycine treatment shows significantly improved outcome and tended to decrease the 30 day mortality of stroke patients . However, how glycine exerts its neuroprotective effect is largely unknown.
The tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) is a dual-specificity phosphatase [15, 16]. PTEN is involved in the regulation of several basic cellular functions, such as cell cycle progression, cell migration, cell spreading and cell growth . We have previously shown that suppressing PTEN confers neuroprotection . Down-regulation of PTEN is shown to protect against neuronal death through the enhancement of Akt activation, the inhibition of NR2B-containing NMDA receptors , the preservation of GABAA receptors and the up-regulation of TDP-43 [19, 20]. We have also shown that NR2A-containing NMDA receptors and DJ-1 exert their neuroprotective effects by down-regulating PTEN [20, 21]. Consistent with our findings, recent studies have revealed that PTEN down-regulation is neuroprotective in cerebral ischemia, traumatic CNS injury and axon regeneration [22–36].
MicroRNAs (miRNAs) are a class of non-coding RNAs (∼22 nucleotides) that negatively regulate protein expression [37, 38]. MiRNAs regulate the expression of at least one third of the human genome and play a critical role in a variety of normal biological processes including cell differentiation, apoptosis, cell development and cell metabolism [37, 39, 40]. In animals, miRNAs regulate mRNA translation via imperfect pairing with nucleotide sequences within the 3′-untranslated region (3′UTR) of target genes .
MiRNA-301a (miR-301a) is the member of miR-130/301a family. miR-301a has been shown to involve in some biological and pathological processes, including cell development, cell differentiation, inflammation, apoptosis and cancer [42–45]. miR-301a is up-regulated in pancreatic cancer and to activate NF-kB by negative regulation of NF-kB-repressing factor gene . miR-301a contributes to IL-6-induced insulin resistance by direct regulation of PTEN and downstream Akt signaling . Recent studies demonstrate that miR-301a promotes cancer cell metastasis in breast, hepatocellular and gastric tumors through different target genes [43, 44, 48]. However, it is not clear whether miR-301a plays a role in neuronal survival.
In the present study, we demonstrate that glycine-induced neuroprotection depends on miR-301a and its downstream signaling. We show that the expression of miR-301a is elevated by glycine. miR-301a mediates glycine-induced neuroprotective effect in cultured cortical neurons subjected to oxygen-glucose deprivation (OGD). miR-301a binds the predicted 3′UTR target sites of PTEN and reduces PTEN expression. We provide evidence that glycine-induced neuroprotection is mediated through the up-regulation of miR-301a and subsequent suppression of PTEN expression.
Glycine is neuroprotective in OGD-induced cortical neuronal injury
Glycine increases miR-301a expression in cortical neurons
miR-301a is neuroprotective in OGD-induced neuronal injury
To determine whether miR-301a is neuroprotective, the cortical neurons were treated with miR-301a agomir or agomir control. At 24 h after the treatment, the neurons were subjected to OGD for 1 h. At 24 h after OGD insult, we found that treatment of miR-301a agomir, but not the agomir control, protected against OGD-induced neuronal death after OGD insult (Fig. 4c). In the same experimental protocols, treatment of miR-301a antagomir but not antagomir control increased OGD-induced neuronal death (Fig. 4d). These results provides the first evidence that miR-301a is neuroprotective in neuronal injury.
miR-301a is required for glycine-induced neuroprotection
PTEN is a target gene of miR-301a
PTEN is regulated by miR-301a in cortical neurons
miR-301a suppresses PTEN to mediate glycine-induced neuroprotection
The cortical neurons were also transduced with lentiviral PTEN siRNAs. At 48 h after transduction, the neurons were subjected to OGD for 1 h. At 1 h after OGD insult the neurons were treated with glycine (100 μM) and collected for cell death and viability assays at 24 h following OGD insult. We found that pre-suppression of PTEN by the siRNA did not significantly enhance glycine-induced neuroprotective effect (Fig. 8b).
The cortical neurons were then treated with lentiviral PTEN siRNA or miR-301a antagomir. The neurons were first treated with lentiviral PTEN siRNA. At 24 h after the transduction, the neurons were treated with miR-301a antagomir or antagomir control. At 24 h after the treatment of antagomir or antagomir control, the neurons were subjected to OGD for 1 h. At 1 h after OGD insult the neurons were treated with glycine (100 μM) and collected for cell death and viability assays at 24 h after OGD insult. We demonstrated that PTEN down-regulation by PTEN siRNA prevented miR-301a antagomir from blocking glycine-induced neuroprotection (Fig. 8c). Taken together, we conclude that miR-301a targets PTEN to mediate glycine-induced neuroprotection.
Glycine is shown to confer protection against neuronal injuries in both in vitro and in vivo experimental conditions [10–13, 49, 50]. Importantly, clinical trial has shown that glycine treatment improves outcome of ischemic stroke patients . However, how glycine exerts its neuroprotective effect remains to be elucidated. The present study provides new evidence that the neuroprotective effect of glycine is mediated by the enhancement of miR-301a expression and subsequent suppression of PTEN expression in cortical neurons. These findings reveal a molecular mechanism by which miRNA-dependent signaling mediates glycine-induced neuroprotection. It is not yet clear how miR301a is regulated by glycine. The expression level of biologically active mature miRNAs is the result of a fine mechanism of biogenesis, carried out by different enzymatic complexes that exert their function at transcriptional and post-transcriptional levels . It is possible that glycine may regulate the processing of miRNA biogenesis. In the cytoplasm, Dicer is an RNase III that digests the pre-miRNA into a 20–25 nucleotides mature duplex miRNA . For example, TAp63, a p53 family member, has been reported to coordinately regulate Dicer and miR-130b to suppress metastasis . TAp63 binds to and transactivates the Dicer promoter, resulting in a direct transcriptional regulation of Dicer by TAp63 . We would reason that glycine may regulate miR301a through a transcriptional mechanism by which Dicer is modulated. Further studies will be performed to test this hypothesis.
As glycine is a co-agonist of NMDA receptors, regulation of miR301a by glycine may be mediated by NMDA receptor-dependent signaling. Previous study has provided evidence that the activation of GluN2A-containing NMDA receptors, but not GluN2B-containing NMDA receptors, contributes to glycine-induced neuroprotective effect in ischemic injury [10, 50]. These studies show that glycine induces its neuroprotection through enhancement of Akt activation and CREB phosphorylation. Thus, it is necessary to investigate next whether GluN2A-containing NMDA receptor-dependent Akt/forkhead/FOXO and/or CREB signaling mediate glycine-induced modulation of miR-301a.
Recent studies indicate that miR-301a plays a critical role in tumorigenesis [43, 44, 46, 48]. It has been reported that miR-301a is up-regulated in hepatocellular carcinoma and modulates NF-kB expression by negatively regulating Gax . miR-301a is also up-regulated in gastric tumor cells and involved in the clinical progression and prognosis of gastric cancer . miR-301a promotes migration and invasion by targeting TGFBR2 in human colorectal cancer . The up-regulation of miR-301a in breast cancer and Ewing’s sarcoma cells promotes tumor metastasis and tumor cell proliferation by targeting the tumor suppressor PTEN [44, 54]. Through negative regulation of SMAD4, miR-301a promotes pancreatic cancer progression . In addition to its effect on cancer, miR-301a is shown to play an important role in regulating the expression of Kv4.2 in diabetes  and controlling autoimmune demyelination by regulating the T-helper 17 immune response . Our study for the first time demonstrates that miR-301a exerts a neuroprotective effect in OGD-induced neuronal injury, implicating a therapeutic potential of miR-301a signaling in neurological diseases. Interestingly, we reveal that glycine acts as an upstream regulator to enhance miR-301a expression, which confer neuroprotection in cortical neurons.
We and others have previously revealed that down-regulation of PTEN is neuroprotective in cerebral ischemia, traumatic CNS injury and axon regeneration [16, 18, 20, 22–36]. We have shown for the first time that depressing PTEN protects against ischemic neuronal death through the enhancement of Akt activation, the inhibition of NR2B-containing NMDA receptors, and the preservation of GABAA receptors [18, 19]. We also show that PTEN suppression prevents excitotoxicity-induced neuronal death via the up-regulation of nuclear TDP-43 . To understand how PTEN is regulated in the neuronal injury process, we have revealed that NR2A-containing NMDA receptors and DJ-1 act as the negative regulators of PTEN to exert their neuroprotective effects . In the present study, we provide new evidence that PTEN is negatively regulated by miR-301a and that PTEN suppression by miR-301a up-regulation mediates glycine-induced neuroprotective effect. Thus, we identify a molecular mechanism by which the glycine/miR-301a signal pathway negatively regulates PTEN in conferring neuroprotection.
Neuronal culture and OGD insult
The cortical neuronal cultures were prepared from Sprague–Dawley rats at gestation day 17 as described [58, 59]. Briefly, dissociated neurons were suspended in plating medium (Neurobasal medium, 2 % B-27 supplement, 0.5 % FBS, 0.5 μM L-glutamine, and 25 μM glutamic acid) and plated on poly-D-lysine coated Petri dishes. After 1 day in culture, half of the plating medium was removed and replaced with maintenance medium (Neurobasal medium, 2 % B-27 supplement, and 0.5 μM L-glutamine). Thereafter, maintenance medium was changed in the same manner every 3 days. The cultured neurons were used for experiments at 12 days after plating.
To initiate the OGD challenge, cells were transferred to deoxygenated glucose-free extracellular solution (ECS) (in mM: 116 NaCl, 5.4 KCl, 0.8 MgSO4, 1.0 NaH2PO4, 1.8 CaCl2, and 26 NaHCO3), introduced into a specialized chamber (Plas-Labs, Lansing, MI), and maintained at 37 °C in 85 % N2/10 % H2/5 % CO2 for 1 h. Neurons were removed from the chamber, transferred to maintenance medium, and returned to the incubator. For “sham” treatment, cultures were transferred to the ECS (in mM: 116 NaCl, 5.4 KCl, 0.8 MgSO4, 1.0 NaH2PO4, 1.8 CaCl2, 26 NaHCO3, and 33 glucose), introduced into the chamber, and maintained at 37 °C for 1 h in 95 % O2/5 % CO2. After the sham treatment, the neurons were transferred to maintenance medium and returned to the original incubator.
The lactate dehydrogenase (LDH) is a cytoplasmic enzyme retained by viable cells with intact plasma membranes and released from cells with damaged membranes. The LDH release was measured using CytoTox 96 Cytotoxicity kit based on the manufacturer’s instructions (Promega, USA). The levels of maximal LDH release were measured by treating the cultures with 10× lysis solution (provided by the manufacturer) to yield complete lysis of the cells. Absorbance data were obtained using a 96-well plate reader (Molecular Devices, USA) at 490 nm. According to the manufacturer’s instructions, the LDH release (%) was calculated by calculating the ratio of experimental LDH release to maximal LDH release.
The viability of the cells in the neuronal cultures was assessed by their ability to uptake thiazolyl blue tetrazolium bromide (MTT). The cells were incubated with MTT for 1 h, then lysed with dimethyl sulfoxide (DMSO) and left at room temperature in the dark overnight. The lysates were then read on a plate reader (PowerWave X, Bio-Tek) at the absorbance wavelength of 540 nm.
Labeling of cortical neurons by fluorescein diacetate
Fluorescein diacetate (FDA) was performed to detect neuronal viability. Briefly, cultures were rinsed with extracellular solution and incubated with FDA (5 μM) for 30 min. The cultures were washed with extracellular solution and then viewed on an Olympus fluorescent microscope (IX51, Olympus).
miRNA microarray assay
Cultured rat cortical neurons treated with or without glycine (100 μM) for 24 h were collected for miRNA microarray analysis. Total RNAs were isolated using TRIzol reagent (Invitrogen, USA) and a miRNeasy Mini Kit (QIAGEN, Germany). RNA samples were applied to the miScript miRNA PCR Arrays (QIAGEN, Germany). Expression profiling was done with dissociation curves using ABI QuantStudio 6 K Flex (ABI, USA). 500 ng RNA template, miScript Reverse Transcriptase Mix (2 μl), 5× miScript HiSpec Buffer (4 μl), 10× miScript Nucleics Mix (2 μl) added into RNase-free water to the volume of 20 μl. Reverse transcription reactions were incubated at 37 °C (60 min) followed by at 95 °C (5 min). The qPCR reactions were consisted of cDNA template (0.8 μl), 2× QuantiTect SYBR Green PCR Master Mix (12.5 μl), 10× miScript Universal Primer (2.5 μl), added into RNase-free water to the volume of 25 μl. Reactions were incubated at 95 °C (15 min), followed by 40 cycles at 94 °C (15 s), 55 °C (30 s) and 70 °C (30 s), followed by melting curve ananlysis. Fold changes in miRNA expression were calculated using the comparative Ct method and normalized to U6 small nuclear RNA (snRNA) as endogenous control. After normalization, differentially expressed miRNAs were identified through Fold Change filtering.
For the quantification of miRNAs or mRNA, total RNAs were isolated using TRIzol reagent (Invitrogen, USA), the reverse transcribed using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo, USA) and the qPCR reaction using SYBR Green qPCR Master mixture (ABI, USA). The reverse transcription was performed on Bio-Rad MJ Mini Instrument (Bio-Rad, USA) and qPCR was performed on QuantStudio 6 K Flex Instrument (ABI, USA).
For the quantification of miRNAs, 2 μg RNA template and 500 nM RT Primer (2 μl) added into RNase-free water to the volume of 11 μl, were incubated at 70 °C (10 min) followed by on ice (2 min), then 5× RT Buffer (5 μl), 2.5 mM dNTP Mix (2 μl), 100U RT enzyme (1 μl) added into RNase-free water to the volume of 25 μl. Reverse transcription reactions were incubated at 42 °C (60 min) followed by at 70 °C (10 min). The miRNA qPCR reactions were consisted of SYBR Green qPCR Master mixture (10 μl), cDNA template (2 μl), 5 μM Bulge-Loop miRNA Forward Primer (0.8 μl) and 5 μM Bulge-Loop miRNA Reverse Primer (0.8 μl) added into RNase-free water to the volume of 20 μl. Reactions were incubated at 95 °C (20 s), followed by 40 cycles at 95 °C (10 s), 60 °C (20 s) and 70 °C (10 s), followed by melting curve analysis.
For the quantification of mRNA, 2 μg RNA template, 25 pM oligo (dT) Primer (0. 25 μl), 10 mM dNTP Mix (1 μl), 5× RT Buffer (4 μl), Maxima H Minus Enzyme Mix (1 μl) were added into RNase-free water to the volume of 20 μl. Reverse transcription reactions were incubated at 25 °C (10 min) followed by at 50 °C (15 min) and at 85 °C (5 min). The qPCR reaction mixtures included the SYBR Green qPCR Master mixture (10 μl), cDNA template (2 μl) and 10 μM Forward Primer (0.6 μl) and 10 μM Reverse Primer (0.6 μl) added into RNase-free water to the volume of 20 μl. Reactions were incubated at 95 °C (3 min), followed by 40 cycles at 95 °C (10 s), 60 °C (30 s), followed by melting curve analysis. All reactions were performed in triplicate for each sample tested. The median Ct (Cycle threshold) value was used for analysis. Relative quantities were calculated using the 2-△△Ct method with U6 snRNA/β-actin. Three independent experiments were performed.
Constructs and treatment
The miR-301a agomir, agomir control, antagomir, antagomir control were purchased from RioBio (China). The cultures were washed with ECS for 10 min, and then treated with serum-free medium supplemented with 1.0 μM agomir, agomir control, antagomir or antagomir control for 2 h at 5 % CO2, 95 % humidity and 37 °C. After incubation, the cultures were further cultured in maintenance medium for 24 h .
The lentiviral PTEN cDNA particles and lentiviral PTEN siRNA particles were purchased from GeneChem (China). The treatment of particles was performed in cultured cortical neurons based on the manufacturer’s instructions.
A 589 bp fragment of the PTEN mRNA-3′UTR was amplified from cDNA by PCR and cloned into pMIR-REPORT Luciferase vector (Invitrogen, USA) to make pMIR-PTEN-3′UTR reporter construct. The primes were 5′-GGACTAGT GGATTTACACATTTATATTTGAACA-3′(F) and 5′-CCCAAGCTT TACCTACAGAACGCAGTTCAGGCTA-3′(R). Site-directed mutagenesis was performed to disrupt the sequence within PTEN mRNA-3′UTR which was complementary to the miR-301a (MIMAT0000552) seed region, and the primers were purchased from RioBio (China). The mutated vector was named pMIR-mut-PTEN 3′UTR. The reporter constructs were confirmed by DNA sequencing.
A total of 5 × 104 PC12 cells were seeded in 24-well plates for 24 h and then co-transfected with 100 ng pMIR-PTEN-3′UTR constructs using Lipofectamine 2000 (Invitrogen, USA) with 1.0 μM miR-301a agomir or agomir control, or with the miR-301a antagomir or antagomir control. Renilla luciferase pRL-TK reporter (Promega, USA) was co-transfected to monitor the transfection efficiency. At 24 h post-transfection, the luciferase activities were measured by the dual-luciferase reporter assay system using the Glomax2020 luminometer (Promega, USA). Data were normalized by Renilla luciferase. Each experiment was performed at least three times in triplicate wells.
Western blotting assay was performed as described previously [18, 58]. For the detection of phospho-Akt, the samples prepared in the same day were used. The polyvinylidene difluoride membrane (Millipore, USA) was incubated with primary antibody against PTEN and β-actin (Cell Signaling, USA). Primary antibodies were labeled with horseradish peroxidase-conjugated secondary antibody, and protein bands were imaged using SuperSignal West Femto Maximum Sensitivity Substrate (Pierce, USA). The EC3 Imaging System (UVP, USA) was used to obtained blot images directly from the polyvinylidene difluoride membrane. The quantification of Western blot data was performed using ImageJ software.
Student’s t test or ANOVA test was used where appropriate to examine the statistical significance of the differences between groups of data. Newman–Keuls tests were used for post-hoc comparisons when appropriate. All results are presented as mean ± SE. Significance was placed at P < 0.05.
Our results indicate that microRNA-301a is neuroprotective in oxygen-glucose deprivation-induced neuronal injury. We provide evidence that glycine is an upstream regulator of microRNA-301a. We conclude that glycine confers neuroprotection through microRNA-301a/PTEN signal pathway.
miR-301a, microRNA-301a; OGD, oxygen-glucose deprivation; PTEN, phosphatase and tensin homolog deleted on chromosome 10; 3′UTR, 3′-untranslated region; LDH, lactate dehydrogenase; MTT, thiazolyl blue tetrazolium bromide; FDA, fluorescein diacetate
This work was supported by China Key Project of Basic Research ("973" Project; 2014CB541606), Natural Science Foundation of China (NSFC; 81470599) and Fund of Collaborative Innovation Center for Brain Science to Q.W.
Availability of data and materials
JC designed, participated in most of the experiments and drafted the manuscript. YZ, ZFZ, PJ, SW, CJH, PCH, GMX, GL and YW performed part of the experiments. ZFW and ZQL designed and performed part of the experiments. QW supervised and wrote the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests
Consent for publication
Ethics approval and consent to participate
All animal experiments were approved by the IACUC of Wuhan University and carried out in compliance with the guidelines of Wuhan University. No human subjects were included in this study.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Kikuchi G, Motokawa Y, Yoshida T, Hiraga K. Glycine cleavage system: reaction mechanism, physiological significance, and hyperglycinemia. Proc Jpn Acad Ser B Phys Biol Sci. 2008;84:246–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Tibbetts AS, Appling DR. Compartmentalization of Mammalian folate-mediated one-carbon metabolism. Annu Rev Nutr. 2010;30:57–81.View ArticlePubMedGoogle Scholar
- Gundersen RY, Vaagenes P, Breivik T, Fonnum F, Opstad PK. Glycine--an important neurotransmitter and cytoprotective agent. Acta Anaesthesiol Scand. 2005;49:1108–16.View ArticlePubMedGoogle Scholar
- Betz H, Laube B. Glycine receptors: recent insights into their structural organization and functional diversity. J Neurochem. 2006;97:1600–10.View ArticlePubMedGoogle Scholar
- Bowery NG, Smart TG. GABA and glycine as neurotransmitters: a brief history. Br J Pharmacol. 2006;147 Suppl 1:S109–19.PubMedPubMed CentralGoogle Scholar
- Lynch JW. Native glycine receptor subtypes and their physiological roles. Neuropharmacology. 2009;56:303–9.View ArticlePubMedGoogle Scholar
- Webb TI, Lynch JW. Molecular pharmacology of the glycine receptor chloride channel. Curr Pharm Des. 2007;13:2350–67.View ArticlePubMedGoogle Scholar
- Dingledine R, Borges K, Bowie D, Traynelis SF. The glutamate receptor ion channels. Pharmacol Rev. 1999;51:7–61.PubMedGoogle Scholar
- Paoletti P. Molecular basis of NMDA receptor functional diversity. Eur J Neurosci. 2011;33:1351–65.View ArticlePubMedGoogle Scholar
- Liu Y, Wong TP, Aarts M, Rooyakkers A, Liu L, Lai TW, et al. NMDA receptor subunits have differential roles in mediating excitotoxic neuronal death both in vitro and in vivo. J Neurosci Off J Soc Neurosci. 2007;27:2846–57.View ArticleGoogle Scholar
- Yang L, Zhang B, Toku K, Maeda N, Sakanaka M, Tanaka J. Improvement of the viability of cultured rat neurons by the non-essential amino acids L-serine and glycine that upregulates expression of the anti-apoptotic gene product Bcl-w. Neurosci Lett. 2000;295:97–100.View ArticlePubMedGoogle Scholar
- Wheeler MD, Thurman RG. Production of superoxide and TNF-alpha from alveolar macrophages is blunted by glycine. Am J Phys. 1999;277:L952–9.Google Scholar
- Zhong Z, Wheeler MD, Li X, Froh M, Schemmer P, Yin M, et al. L-Glycine: a novel antiinflammatory, immunomodulatory, and cytoprotective agent. Curr Opin Clin Nutr Metab Care. 2003;6:229–40.View ArticlePubMedGoogle Scholar
- Gusev EI, Skvortsova VI, Dambinova SA, Raevskiy KS, Alekseev AA, Bashkatova VG, et al. Neuroprotective effects of glycine for therapy of acute ischaemic stroke. Cerebrovasc Dis. 2000;10:49–60.View ArticlePubMedGoogle Scholar
- Maehama T, Dixon JE. The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem. 1998;273:13375–8.View ArticlePubMedGoogle Scholar
- Chang N, El-Hayek YH, Gomez E, Wan Q. Phosphatase PTEN in neuronal injury and brain disorders. Trends Neurosci. 2007;30:581–6.View ArticlePubMedGoogle Scholar
- Tamura M, Gu J, Takino T, Yamada KM. Tumor suppressor PTEN inhibition of cell invasion, migration, and growth: differential involvement of focal adhesion kinase and p130Cas. Cancer Res. 1999;59:442–9.PubMedGoogle Scholar
- Ning K, Pei L, Liao M, Liu B, Zhang Y, Jiang W, et al. Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN. J Neurosci Off J Soc Neurosci. 2004;24:4052–60.View ArticleGoogle Scholar
- Liu B, Li L, Zhang Q, Chang N, Wang D, Shan Y, et al. Preservation of GABAA receptor function by PTEN inhibition protects against neuronal death in ischemic stroke. Stroke. 2010;41:1018–26.View ArticlePubMedGoogle Scholar
- Zheng M, Liao M, Cui T, Tian H, Fan DS, Wan Q. Regulation of nuclear TDP-43 by NR2A-containing NMDA receptors and PTEN. J Cell Sci. 2012;125:1556–67.View ArticlePubMedPubMed CentralGoogle Scholar
- Chang N, Li L, Hu R, Shan Y, Liu B, Wang H, et al. Differential regulation of NMDA receptor function by DJ-1 and PINK1. Aging Cell. 2010;9:837–50.View ArticlePubMedGoogle Scholar
- Lee SM, Zhao H, Maier CM, Steinberg GK. The protective effect of early hypothermia on PTEN phosphorylation correlates with free radical inhibition in rat stroke. J Cereb Blood Flow Metab. 2009;29:1589–600.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu C, Wu J, Xu K, Cai F, Gu J, Ma L, et al. Neuroprotection by baicalein in ischemic brain injury involves PTEN/AKT pathway. J Neurochem. 2010;112:1500–12.View ArticlePubMedGoogle Scholar
- Walker CL, Xu XM. PTEN inhibitor bisperoxovanadium protects oligodendrocytes and myelin and prevents neuronal atrophy in adult rats following cervical hemicontusive spinal cord injury. Neurosci Lett. 2014;573:64–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Goncalves MB, Malmqvist T, Clarke E, Hubens CJ, Grist J, Hobbs C, et al. Neuronal RARbeta Signaling Modulates PTEN Activity Directly in Neurons and via Exosome Transfer in Astrocytes to Prevent Glial Scar Formation and Induce Spinal Cord Regeneration. J Neurosci Off J Soc Neurosci. 2015;35:15731–45.View ArticleGoogle Scholar
- Ohtake Y, Hayat U, Li S. PTEN inhibition and axon regeneration and neural repair. Neural regeneration Res. 2015;10:1363–8.View ArticleGoogle Scholar
- Du K, Zheng S, Zhang Q, Li S, Gao X, Wang J, et al. Pten Deletion Promotes Regrowth of Corticospinal Tract Axons 1 Year after Spinal Cord Injury. J Neurosci Off J Soc Neurosci. 2015;35:9754–63.View ArticleGoogle Scholar
- Geoffroy CG, Lorenzana AO, Kwan JP, Lin K, Ghassemi O, Ma A, et al. Effects of PTEN and Nogo Codeletion on Corticospinal Axon Sprouting and Regeneration in Mice. J Neurosci Off J Soc Neurosci. 2015;35:6413–28.View ArticleGoogle Scholar
- Lewandowski G, Steward O. AAVshRNA-mediated suppression of PTEN in adult rats in combination with salmon fibrin administration enables regenerative growth of corticospinal axons and enhances recovery of voluntary motor function after cervical spinal cord injury. J Neurosci Off J Soc Neurosci. 2014;34:9951–62.View ArticleGoogle Scholar
- Singh B, Singh V, Krishnan A, Koshy K, Martinez JA, Cheng C, et al. Regeneration of diabetic axons is enhanced by selective knockdown of the PTEN gene. Brain. 2014;137:1051–67.View ArticlePubMedPubMed CentralGoogle Scholar
- Christie KJ, Webber CA, Martinez JA, Singh B, Zochodne DW. PTEN inhibition to facilitate intrinsic regenerative outgrowth of adult peripheral axons. J Neurosci Off J Soc Neurosci. 2010;30:9306–15.View ArticleGoogle Scholar
- Howitt J, Lackovic J, Low LH, Naguib A, Macintyre A, Goh CP, et al. Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia. J Cell Biol. 2012;196:29–36.View ArticlePubMedPubMed CentralGoogle Scholar
- Goh CP, Putz U, Howitt J, Low LH, Gunnersen J, Bye N, et al. Nuclear trafficking of Pten after brain injury leads to neuron survival not death. Exp Neurol. 2014;252:37–46.View ArticlePubMedGoogle Scholar
- Sun F, Park KK, Belin S, Wang D, Lu T, Chen G, et al. Sustained axon regeneration induced by co-deletion of PTEN and SOCS3. Nature. 2011;480:372–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Park KK, Liu K, Hu Y, Smith PD, Wang C, Cai B, et al. Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science. 2008;322:963–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu K, Lu Y, Lee JK, Samara R, Willenberg R, Sears-Kraxberger I, et al. PTEN deletion enhances the regenerative ability of adult corticospinal neurons. Nat Neurosci. 2010;13:1075–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116:281–97.View ArticlePubMedGoogle Scholar
- Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol. 2005;6:376–85.View ArticlePubMedGoogle Scholar
- Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ, Plasterk RH. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 2001;15:2654–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Lee Y, Kim M, Han J, Yeom KH, Lee S, Baek SH, et al. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 2004;23:4051–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Doench JG, Sharp PA. Specificity of microRNA target selection in translational repression. Genes Dev. 2004;18:504–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Lee YH, Na HS, Jeong SY, Jeong SH, Park HR, Chung J, et al. Comparison of inflammatory microRNA expression in healthy and periodontitis tissues. Biocell. 2011;35:43–9.PubMedGoogle Scholar
- Wang M, Li C, Yu B, Su L, Li J, Ju J, et al. Overexpressed miR-301a promotes cell proliferation and invasion by targeting RUNX3 in gastric cancer. J Gastroenterol. 2013;48:1023–33.View ArticlePubMedGoogle Scholar
- Ma F, Zhang J, Zhong L, Wang L, Liu Y, Wang Y, et al. Upregulated microRNA-301a in breast cancer promotes tumor metastasis by targeting PTEN and activating Wnt/beta-catenin signaling. Gene. 2014;535:191–7.View ArticlePubMedGoogle Scholar
- Xu XD, He XJ, Tao HQ, Zhang W, Wang YY, Ye ZY, et al. Abnormal expression of miR-301a in gastric cancer associated with progression and poor prognosis. J Surg Oncol. 2013;108:197–202.View ArticlePubMedGoogle Scholar
- Lu Z, Li Y, Takwi A, Li B, Zhang J, Conklin DJ, et al. miR-301a as an NF-kappaB activator in pancreatic cancer cells. EMBO J. 2011;30:57–67.View ArticlePubMedPubMed CentralGoogle Scholar
- Dou L, Wang S, Sui X, Meng X, Shen T, Huang X, et al. MiR-301a mediates the effect of IL-6 on the AKT/GSK pathway and hepatic glycogenesis by regulating PTEN expression. Cell Physiol Biochem. 2015;35:1413–24.View ArticlePubMedGoogle Scholar
- Zhang W, Zhang T, Jin R, Zhao H, Hu J, Feng B, et al. MicroRNA-301a promotes migration and invasion by targeting TGFBR2 in human colorectal cancer. J Exp Clin Cancer Res. 2014;33:113.View ArticlePubMedPubMed CentralGoogle Scholar
- Tanabe M, Nitta A, Ono H. Neuroprotection via strychnine-sensitive glycine receptors during post-ischemic recovery of excitatory synaptic transmission in the hippocampus. J Pharmacol Sci. 2010;113:378–86.View ArticlePubMedGoogle Scholar
- Chen Z, Hu B, Wang F, Du L, Huang B, Li L, et al. Glycine bidirectionally regulates ischemic tolerance via different mechanisms including NR2A-dependent CREB phosphorylation. J Neurochem. 2015;133:397–408.View ArticlePubMedGoogle Scholar
- Ha M, Kim VN. Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 2014;15:509–24.View ArticlePubMedGoogle Scholar
- Su X, Chakravarti D, Cho MS, Liu L, Gi YJ, Lin YL, et al. TAp63 suppresses metastasis through coordinate regulation of Dicer and miRNAs. Nature. 2010;467:986–90.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou P, Jiang W, Wu L, Chang R, Wu K, Wang Z. miR-301a is a candidate oncogene that targets the homeobox gene Gax in human hepatocellular carcinoma. Dig Dis Sci. 2012;57:1171–80.View ArticlePubMedGoogle Scholar
- Kawano M, Tanaka K, Itonaga I, Iwasaki T, Tsumura H. MicroRNA-301a promotes cell proliferation via PTEN targeting in Ewing’s sarcoma cells. Int J Oncol. 2016.Google Scholar
- Xia X, Zhang K, Cen G, Jiang T, Cao J, Huang K, et al. MicroRNA-301a-3p promotes pancreatic cancer progression via negative regulation of SMAD4. Oncotarget. 2015;6:21046–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Panguluri SK, Tur J, Chapalamadugu KC, Katnik C, Cuevas J, Tipparaju SM. MicroRNA-301a mediated regulation of Kv4.2 in diabetes: identification of key modulators. PLoS One. 2013;8:e60545.View ArticlePubMedPubMed CentralGoogle Scholar
- Mycko MP, Cichalewska M, Machlanska A, Cwiklinska H, Mariasiewicz M, Selmaj KW. MicroRNA-301a regulation of a T-helper 17 immune response controls autoimmune demyelination. Proc Natl Acad Sci U S A. 2012;109:E1248–57.View ArticlePubMedPubMed CentralGoogle Scholar
- Shan Y, Liu B, Li L, Chang N, Li L, Wang H, et al. Regulation of PINK1 by NR2B-containing NMDA receptors in ischemic neuronal injury. J Neurochem. 2009;111:1149–60.View ArticlePubMedGoogle Scholar
- Brewer GJ, Torricelli JR, Evege EK, Price PJ. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res. 1993;35:567–76.View ArticlePubMedGoogle Scholar
- Haftmann C, Riedel R, Porstner M, Wittmann J, Chang HD, Radbruch A, et al. Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes. J Immunol Methods. 2015;426:128–33.View ArticlePubMedPubMed CentralGoogle Scholar