Fig. 6

ChIP analysis of putative AP-2 binding sites located upstream of the Brn3c gene. P1 mouse retinal tissue was cross-linked and genomic DNA/AP-2δ complexes immunoprecipitated with anti-AP-2δ antibody. DNA purified from cross-linked complexes was PCR amplified using primer pairs flanking three putative AP-2 binding sites located within 3 kb of the Brn3c transcription start site. Normal rabbit IgG served as the negative control. Input DNA is the DNA following sonication but prior to immunoprecipitation. Results are shown from two experiments. Note that the second gel shown for site 1 was prepared using agarose rather than acrylamide. The intensity of the DNA signal was quantified by densitometric analysis using Adobe Photoshop and background subtraction. Values indicate the average of 2 independent experiments, with signal density in the IgG lanes set at 1