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Table 1 Primers for single-cell RT-PCR

From: Expression of functional inhibitory neurotransmitter transporters GlyT1, GAT-1, and GAT-3 by astrocytes of inferior colliculus and hippocampus

Gene Sequence Expected amplicon size [bp] GeneBank accession number
GlyT1 fwd 5’-CACCAACTGTGCTACCAGCGTCTA-3′
rev 5’-GCATAGTTGTCCATCAGCAGCAGC-3’
390 NM_008135.4
GlyT1 (nested) fwd 5’-GCTTCGTCATCTTCTCCATCCTG-3′
rev 5’-CAGTAGATGCCTGCCTGGCTG-3’
337
GlyT2 fwd 5’-TCCTGTGTTATCGGTGACCATCC-3′
rev 5’-GAGTGGCCGCATCCTTCCATA-3’
430 NM_148931.3
GlyT2 (nested) fwd 5’-TCTGCATGACTGCCTATCCGAACT-3′
rev 5’-TGTGATGAAGTACCAGATGCCGG-3’
329
GAT-1 fwd 5’-GATGACAGATGGACTGGACAAG-3′
rev 5’-CACGATGGAGAAGATGACGAAT-3’
430 NM_178703.4
GAT-1 (nested) fwd 5‘-TTGGACTGGAAAGGTGGTCTA-3‘
rev 5’-ACGATGGAGAAGATGACGAATC-3’
324
GAT-2 fwd 5’-TTATTGTGTCCGTCATCTCGTT-3′
rev 5’-ACTTCTTGTTGTAGGTCAGTGG-3’
309 NM_144512.2
GAT-2 (nested) fwd 5’-GTTCTTCATCGGGCTCATCAT-3′
rev 5’-TAGGTCAGTGGCGTGTATTTG-3’
279
GAT-3 fwd 5’-GGGCATCTTCATCTTCTTTCTG-3′
rev 5’-GGTTCAGGATTCATTTACACGC-3’
390 NM_172890.3
GAT-3 (nested) fwd 5’-GGGCATCTTCATCTTCTTTCTG-3′
rev 5’-AGTGTGTCTCCTTCTCTGTGAT-3’
320
BGT-1 fwd 5’-ACTTTCTTCTTCTCCTTGAGCA-3′
rev 5’-CTCTGGCACTTCCTACAAATGA-3’
324 NM_133661.3
BGT-1 (nested) fwd 5’-ACTTTCTTCTTCTCCTTGAGCA-3′
rev 5’-ATGAGTTCTTGTTTGGCTGGA-3’
284
Kir4.1 fwd 5’-ACT TTC TTC TTC TCC TTG AGCA-3′
rev 5’-CTC TGG CAC TTC CTA CAA ATGA-3’
324 NM_001039484.1
Kir4.1 (nested) fwd 5’-ACT TTC TTC TTC TCC TTG AGCA-3′
rev 5′-ATG AGT TCT TGT TTG GCT GGA-3’
284
  1. fwd Forward (/sence) primer, rev Reverse (/antisence) primer, bp Base pairs