Table 1 Primers for single-cell RT-PCR
Gene | Sequence | Expected amplicon size [bp] | GeneBank accession number |
|---|---|---|---|
GlyT1 | fwd 5’-CACCAACTGTGCTACCAGCGTCTA-3′ rev 5’-GCATAGTTGTCCATCAGCAGCAGC-3’ | 390 | NM_008135.4 |
GlyT1 (nested) | fwd 5’-GCTTCGTCATCTTCTCCATCCTG-3′ rev 5’-CAGTAGATGCCTGCCTGGCTG-3’ | 337 | |
GlyT2 | fwd 5’-TCCTGTGTTATCGGTGACCATCC-3′ rev 5’-GAGTGGCCGCATCCTTCCATA-3’ | 430 | NM_148931.3 |
GlyT2 (nested) | fwd 5’-TCTGCATGACTGCCTATCCGAACT-3′ rev 5’-TGTGATGAAGTACCAGATGCCGG-3’ | 329 | |
GAT-1 | fwd 5’-GATGACAGATGGACTGGACAAG-3′ rev 5’-CACGATGGAGAAGATGACGAAT-3’ | 430 | NM_178703.4 |
GAT-1 (nested) | fwd 5‘-TTGGACTGGAAAGGTGGTCTA-3‘ rev 5’-ACGATGGAGAAGATGACGAATC-3’ | 324 | |
GAT-2 | fwd 5’-TTATTGTGTCCGTCATCTCGTT-3′ rev 5’-ACTTCTTGTTGTAGGTCAGTGG-3’ | 309 | NM_144512.2 |
GAT-2 (nested) | fwd 5’-GTTCTTCATCGGGCTCATCAT-3′ rev 5’-TAGGTCAGTGGCGTGTATTTG-3’ | 279 | |
GAT-3 | fwd 5’-GGGCATCTTCATCTTCTTTCTG-3′ rev 5’-GGTTCAGGATTCATTTACACGC-3’ | 390 | NM_172890.3 |
GAT-3 (nested) | fwd 5’-GGGCATCTTCATCTTCTTTCTG-3′ rev 5’-AGTGTGTCTCCTTCTCTGTGAT-3’ | 320 | |
BGT-1 | fwd 5’-ACTTTCTTCTTCTCCTTGAGCA-3′ rev 5’-CTCTGGCACTTCCTACAAATGA-3’ | 324 | NM_133661.3 |
BGT-1 (nested) | fwd 5’-ACTTTCTTCTTCTCCTTGAGCA-3′ rev 5’-ATGAGTTCTTGTTTGGCTGGA-3’ | 284 | |
Kir4.1 | fwd 5’-ACT TTC TTC TTC TCC TTG AGCA-3′ rev 5’-CTC TGG CAC TTC CTA CAA ATGA-3’ | 324 | NM_001039484.1 |
Kir4.1 (nested) | fwd 5’-ACT TTC TTC TTC TCC TTG AGCA-3′ rev 5′-ATG AGT TCT TGT TTG GCT GGA-3’ | 284 |