Helix-A peptide prevents gp120-mediated neuronal loss
Molecular Brain volume 12, Article number: 61 (2019)
The human-immunodeficiency virus (HIV) envelope protein gp120 promotes synaptic damage similar to that observed in people living with HIV who have neurocognitive disorders. The neurotoxic effect of gp120 appears to occur through the α-helix motif that binds to neuronal microtubules (MTs). In this study, we examined the ability of short peptide derivatives from Helix-A, a peptide synthesized based on α-helix structure of gp120, to displace gp120 from binding to MTs and prevent its neurotoxic effects.
Surface plasmon resonance was used to determine the binding of Helix-A and its modifications to MTs. Helix-A peptide and derivatives were delivered inside rat primary cortical neurons by mesoporous silica nanoparticles (MSN). Neuronal processes and survival were evaluated by microtubule associated protein 2-immunostaining and Hoechst/Propidium iodide, respectively.
Surface plasmon resonance analysis revealed that Helix-A but not its modifications binds to MTs. Also, only Helix-A MSN but not other peptides prevented the ability of gp120 to reduce neuronal processes as well as neuronal survival. Thus, the amino acid structure of Helix-A is key for its neuroprotective activity.
Various degrees of axonal and dendritic pathology are seen in human-immunodeficiency virus (HIV) positive subjects who develop HIV-associated neurocognitive disorders (HAND) [1, 2]. This neurological consequence of HIV infection of the brain occurs also in the HIV-infected population undergoing the antiretroviral therapy . Thus, new therapeutic strategies to reduce neuronal loss are needed. However, more knowledge about the mechanisms of how HIV is neurotoxic is crucial for a better adjunct therapy.
HIV proteins are, among other causes, most likely responsible for the damage of synapses. In particular, the envelope protein gp120, which can be secreted by HIV-infected cells, induces neuronal apoptosis through direct binding to chemokine receptors, CXCR4 and CCR5 [4, 5]. Binding of gp120 to these receptors promotes its rapid endocytosis by neurons . Importantly, internalized gp120 binds to microtubules (MTs) [7, 8], which are the major avenue for intracellular transport of many organelles, including mitochondria, and synaptic vesicles to distal axons and dendrites. Changes in the integrity of MTs are sufficient to alter proper energy supply within neurites and synapses leading to acute or chronic axonal and dendritic fragmentation . Thus, gp120 by binding to MTs may disrupting their function and decrease neuronal survival.
We have previously provided evidence that the conserved α-helix region of gp120 binds to the carboxy terminal tail of class III beta tubulin (TUBB3), one of the MT elements found predominantly in neurons . When such binding is blocked by a 19-amino acid peptide, termed Helix-A, synthetized based on the α-helix motif of gp120, the neurotoxic effect of the envelope protein is abolished . In this work, we have analyzed different peptides carrying various amino acid mutations to investigate whether the α-helix motif is necessary for the neuroprotective activity of Helix-A.
To examine the structure of Helix-A required to displace gp120 from binding to TUBB3, we designed and synthetized peptides to carry mutations so that either the helix motif of Helix-A is lost or specific amino acid sequences are changed (Fig. 1a). These include peptides with a truncated C-terminal (NDMVEQMHED, modification #1) or N-terminal (IISLWDQSLK, modification #2) of Helix-A. These modifications produce peptides with shorter helical structure. In addition, we synthetized peptides in which glycine (modification #3) or proline (modification #4) were introduced into the original Helix-A peptide to completely distrupt the α-helix conformation. We also synthetized Helix-B peptide, which has a α-helix structure but it does not displace gp120 from MTs . Analysis of interaction of peptides to assembled MTs, using surface plasmon resonance, indicated that only the unmodified Helix-A peptide binds to assembled MTs (Fig. 1a). Binding of Helix-A peptide to TUBB3 was in the micromole range; however, this is consistent with a rapid dissociation of Helix-A from MTs due to its small mass (~ 1696 Da). Nevertheless, our data support the suggestion that the amino-acid sequence as well as the α-helix structure of Helix-A peptide are essential for its binding to MTs.
We have previously shown that Helix-A peptide cannot cross cell membranes unless cross-linked to mesoporous silica nanoparticles (MSN) . Once inside neurons, Helix-A MSN has neuroprotective effect against at least two strains of gp120: T-tropic (gp120IIIB) and M-tropic (gp120ADA) . To examine whether Helix-A peptide modifications are neuroprotective, we cross-linked all modified peptides to MSN. We then used these peptides to test whether they prevent gp120ADA-mediated neurite pruning and cell loss in rat cortical cultures. MSN alone or peptides crosslinked to MSN alone did not affect either neurite lengths, determined by microtubule associated protein 2 (MAP2) staining, or neuronal survival measured by Hoechst/propidium iodide (PI) (data not shown), suggesting that these compounds have no side effects tha impair neuronal viability. However, Helix-A MSN but not all modified peptide-MSN prevented gp120-mediated neurite pruning (Fig. 1b), as well as neuronal loss (Fig. 1c). It is important to note that Helix-A not crosslinked to MSN or Helix-B MSN were ineffective in blocking gp120 neurotoxicity . These data support the hypothesis that the amino acid sequence of the Helix-A, which binds to MTs, is crucial for the neuroprotective activity against gp120.
MTs are composed of tubulin heterodimers comprised of repeats of α- and β-tubulin, which are conserved among all eukaryotic species . TUBB3 contains multiple α-helix motifs. The secondary structure of gp120 also contains three α-helix motifs that can determine associations between different proteins . We have previously shown that the α-helix of gp120 near the V3 loop could form a dimer with the α-helix of TUBB3 located in its carboxyl terminal tail . Such binding may alter the stability and function of neuronal MTs. Thus, a binding of gp120 to TUBB3 may impair MTs function. Indeed, we have previously demonstrated that the Helix-A peptide, which displaces gp120 binding to TUBB3 by interrupting the helix-helix interaction of gp120 with TUBB3, is neuroprotective . In this work, we further established that modifications of the amino acid sequence in Helix-A peptide that either disrupt its helical conformation or abolish the binding site as well as the neuroprotective activity against gp120. Nevertheless, we also observed that another α-helix motif (Helix-B) in gp120 secondary structure does not bind to MTs and it is not neuroprotective. Therefore, both the α-helix structure, as well as the amino acid sequence within, are equally important for high affinity binding of gp120 to MTs. Lastly, the amino acid sequence of the α-helix structure is not homologous to that found in the V3 loop domain necessary for the binding of gp120 to the chemokine co-receptors . Thus, our results support the suggestion that activation of these receptors, although necessary to deliver gp120 inside neurons, , may not entirely explain the cellular mechanisms of gp120 neurotoxicity.
The ability of Helix-A to block gp120 neurotoxicity was tested in an in vitro experimental model of HAND. Future studies must test the effect of Helix-A in vivo to ascertain whether this peptide is a suitable approach for a new adjunct therapy against HIV, in addition to CXCR4 or CCR5 receptor antagonists.
Availability of data and materials
All data generated or analyzed during this study are included in this published article.
HIV-associated neurocognitive disorders
microtubule associated protein 2
mesoporous silica nanoparticles
class III beta tubulin
Alakkas A, Ellis RJ, Watson CW, Umlauf A, Heaton RK, Letendre S, et al. White matter damage, neuroinflammation, and neuronal integrity in HAND. J Neuro-Oncol. 2019;25(1):32–41.
Saylor D, Dickens AM, Sacktor N, Haughey N, Slusher B, Pletnikov M, et al. HIV-associated neurocognitive disorder--pathogenesis and prospects for treatment. Nat Rev Neurol. 2016;12(4):234–48.
Chan P, Brew B. HIV associated neurocognitive disorders in the modern antiviral treatment era: prevalence, characteristics, biomarkers, and effects of treatment. Current HIV/AIDS reports. 2014;11(3):317–24.
Meucci O, Fatatis A, Simen AA, Bushell TJ, Gray PW, Miller RJ. Chemokines regulate hippocampal neuronal signaling and gp120 neurotoxicity. Proc Natl Acad Sci U S A. 1998;95(24):14500–5.
Kaul M, Ma Q, Medders KE, Desai MK, Lipton SA. HIV-1 coreceptors CCR5 and CXCR4 both mediate neuronal cell death but CCR5 paradoxically can also contribute to protection. Cell Death Differ. 2007;14(2):296–305.
Berth S, Caicedo HH, Sarma T, Morfini G, Brady ST. Internalization and axonal transport of the HIV glycoprotein gp120. ASN neuro. 2015;7:1–15.
Bachis A, Aden SA, Nosheny RL, Andrews PM, Mocchetti I. Axonal transport of human immunodeficiency virus type 1 envelope protein glycoprotein 120 is found in association with neuronal apoptosis. J Neurosci. 2006;26(25):6771–80.
Avdoshina V, Taraballi F, Dedoni S, Corbo C, Paige M, Saygideger Kont Y, et al. Identification of a binding site of the human immunodeficiency virus envelope protein gp120 to neuronal-specific tubulin. J Neurochem. 2016;137(2):287–98.
Wang JT, Medress ZA, Barres BA. Axon degeneration: molecular mechanisms of a self-destruction pathway. J Cell Biol. 2012;196(1):7–18.
Wenzel ED, Bachis A, Avdoshina V, Taraballi F, Tasciotti E, Mocchetti I. Endocytic trafficking of HIV gp120 is mediated by dynamin and plays a role in gp120 neurotoxicity. J NeuroImmune Pharmacol. 2017;12(3):492–503.
Nogales E, Wolf SG, Downing KH. Structure of the alpha beta tubulin dimer by electron crystallography. Nature. 1998;391(6663):199–203.
Bullock BN, Jochim AL, Arora PS. Assessing helical protein interfaces for inhibitor design. J Am Chem Soc. 2011;133(36):14220–3.
Mocchetti I, Bachis A, Esposito G, Turner SR, Taraballi F, Tasciotti E, et al. Human immunodeficiency virus-associated dementia: a link between accumulation of viral proteins and neuronal degeneration. Current trends in neurology. 2014;8:71–85.
Rizzuto CD, Wyatt R, Hernandez-Ramos N, Sun Y, Kwong PD, Hendrickson WA, et al. A conserved HIV gp120 glycoprotein structure involved in chemokine receptor binding. Science. 1998;280(5371):1949–53.
This work was supported by the National Institute of Health NS 104000. The Biacore Molecular Interaction Shared Resource at the Lombardi Comprehensive Cancer Center (Georgetown University) is supported by a grant P30CA51008 from the National Cancer Institute.
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Animal studies were done in strict accordance with the Laboratory Animal Welfare Act, with National Institutes of Health Guide for the Care and Use of Laboratory Animals following approved animal protocols from Georgetown University Animal Care and Use Committee or National Institute of Environmental Health Sciences.
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Avdoshina, V., Taraballi, F., Tasciotti, E. et al. Helix-A peptide prevents gp120-mediated neuronal loss. Mol Brain 12, 61 (2019). https://doi.org/10.1186/s13041-019-0482-z