Fig. 4

C. elegans TMEM-132 interaction with GEX-3 requires C-terminal domains involving key amino acid residues. a Exemplar Western blot showing CoIP of C. elegans TMEM-132 (mCherry-tagged) and GEX-3 (HA-tagged) C terminal domains. Transgenic expression was driven by hsp-16 promoters that are heat shock inducible by placing transgenic animals at 32 °C for 2 h followed by recovery at 20 °C for 4 h. b Yeast growth colonies showing interaction of C. elegans TMEM-132 Ct and a mutant with a putative WIRS-like motif converted to alanine residues, with GEX-3 full length, mutants with C-terminal 600 a.a. and 60 a.a. fragments. c Yeast growth colonies showing interaction of C. elegans GEX-3 full length, mutants with C-terminal 600 a.a. fragments with TMEM-132 Ct and mutants with substitutions of proline 784 to alanine or threonine, respectively. Double dropout -Leu/-Trp yielded colonies without apparent differences, indicating that these mutations do not affect protein levels