The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex
Molecular Brain volume 14, Article number: 54 (2021)
TMEM132D is a human gene identified with multiple risk alleles for panic disorders, anxiety and major depressive disorders. Defining a conserved family of transmembrane proteins, TMEM132D and its homologs are still of unknown molecular functions. By generating loss-of-function mutants of the sole TMEM132 ortholog in C. elegans, we identify abnormal morphologic phenotypes in the dopaminergic PDE neurons. Using a yeast two-hybrid screen, we find that NAP1 directly interacts with the cytoplasmic domain of human TMEM132D, and mutations in C. elegans tmem-132 that disrupt interaction with NAP1 cause similar morphologic defects in the PDE neurons. NAP1 is a component of the WAVE regulatory complex (WRC) that controls F-actin cytoskeletal dynamics. Decreasing activity of WRC rescues the PDE defects in tmem-132 mutants, whereas gain-of-function of TMEM132D in mammalian cells inhibits WRC, leading to decreased abundance of select WRC components, impaired actin nucleation and cell motility. We propose that metazoan TMEM132 family proteins play evolutionarily conserved roles in regulating NAP1 protein homologs to restrict inappropriate WRC activity, cytoskeletal and morphologic changes in the cell.
Despite decades of genetic and molecular analyses, the genome of the common model organism C. elegans still comprises many functionally uncharacterized genes [1,2,3,4]. One such example is the C. elegans gene Y71H2AM.10, an ortholog of the evolutionarily conserved TMEM132 gene family [1, 5]. This family encodes single-pass transmembrane proteins present in metazoans but remains functionally uncharacterized in any organisms. The human genome encodes 5 paralogs (TMEM132A-E), genetic variants of which have been identified as risk alleles of many human diseases, including those associated with panic disorder and anxiety severity in TMEM132D [6,7,8,9,10,11,12]. Risk variants of TMEM132D have been shown to correlate with altered mRNA levels of TMEM132D in anxiety-related brain regions and psychiatric syndromes [6, 9]. In addition, the TMEM132D locus in cattle appears to have undergone an evolutionary selective sweep during domestication, along with reduced fearfulness in cattle’s behaviors . The expression of TMEM132 family genes is also highly enriched in the nervous system of diverse animals, including C. elegans and humans [14, 15]. However, it remains unknown how TMEM132 family proteins regulate neuronal structure and function and how their abnormal function and regulation may contribute to various neurological and psychiatric diseases.
Neuronal morphological changes are driven primarily by actin cytoskeletal dynamics under the control of the WAVE-regulatory complex (WRC). WRC promotes actin nucleation to form filamentous actin (F-actin) by stimulating activity of the Arp2/3 complex in response to biochemical signals originating from a variety of neuronal membrane receptors [16,17,18]. WRC is a multi-subunit complex comprising SRA1, HSPC300, ABI1/2, WAVE1/2 and NAP1 (also known as NCKAP1) proteins. NAP1 was initially identified as a gene with strongly decreased expression in the brain of patients with sporadic Alzheimer’s disease . Deleterious NAP1 variants were also identified in human patients with autism and intellectual disability [20, 21]. Among other components in the WRC, the SRA and ABI proteins form an evolutionarily conserved binding interface for diverse WRC ligands that commonly contain the WRC-interacting receptor sequence (WIRS) motif [16, 17]. Whether NAP1 directly interacts with any neuronal membrane receptors to affect WRC signaling and actin cytoskeletal changes has not been reported. It has also been unclear how WRC abundance is regulated in cell compartments where actin nucleation needs to be limited in morphologically complex cells, including neurons.
To elucidate biological functions and mechanisms of action of TMEM132 family proteins, we generated and characterized C. elegans loss-of-function (LOF) mutations in Y71H2AM.10, the sole ortholog of the TMEM132 gene family. We found that tmem-132 mutants exhibit striking morphological defects in the dopaminergic PDE neurons. Human NAP1 binds to TMEM132D and their C. elegans homologs also interact with each other. Genetic interactions between tmem-132 and WRC-encoding genes in regulating PDE morphology, the LOF phenotype of tmem-132 mutants and gain-of-function (GOF) phenotype of TMEM132D in mammalian cells collectively suggest that TMEM132 family proteins may regulate NAP1 in WRC to finely modulate actin nucleation, cellular cytoskeletal and morphological changes.
The C. elegans TMEM-132 localizes to neurons and regulates the dopaminergic PDE neuron morphology
Protein homology and motif analysis identified the Pfam16070 domain characteristic of both human TMEM132D and C. elegans TMEM-132, classifying them to the evolutionarily conserved TMEM132 protein superfamily (Fig. 1a and Additional file 1: Fig. S1). A translational reporter that fuses tmem-132 with GFP revealed enriched expression of C. elegans tmem-132 in neurons (Fig. 1b–d), implicating a specific role of TMEM-132 in the nervous system. Lack of mammalian loss-of-function models and potential genetic redundancy among the TMEM132A-E family members precluded us from analyzing the physiological function of TMEM132D in vivo. Thus, we sought to address this issue in C. elegans, which encodes tmem-132 as the sole ortholog of the gene family and has served as excellent model to study neuronal cell biology [22,23,24]. We used CRISPR-Cas9 techniques to generate a series of C. elegans mutants, including multiple independently-derived deletions, an early stop-codon mutant and a genetic P784T knock-in mutant, in which the highly conserved proline residue became threonine, corresponding to the human disease risk allele for anxiety and panic disorders (Fig. 1e, f). We generated such multiple independent mutations to seek convergent phenotype and outcrossed all mutants to eliminate potential interference of phenotype by background mutations.
Given the exclusive localization of TMEM-132 in neurons, we subjected tmem-132 mutants to a variety of neuronal phenotypic analyses. We did not observe gross behavioral defects under normal conditions. We next crossed the mutants to various established GFP reporters to examine neurons of stereotyped morphology, including ciliated sensory AWC neurons, hermaphrodyte-specific HSN neurons, mechanosensory PVD, ADE and PDE neurons (Additional file 1: Fig. S2). Among the neurons examined, the dopaminergic neuron PDE exhibited the most severe defect, thus in this study we focused on PDE, which is marked by the osm-6p::GFP reporter in addition to other ciliated and dopaminergic neurons. Although dense GFP signals prevented us from analyzing the anterior group of ciliated and dopaminergic neurons, close confocal microscopic analysis of the posterior, anatomically isolated PDE neurons revealed striking abnormal morphologies in a large fraction of tmem-132 mutants (Fig. 2a–d). We categorized the mutant phenotype into several classes, including those with irregular soma outlines, ectopic dendrites, ectopic axon branches, and axon misguidance as similarly described previously [25, 26]. Although the phenotypic defects of PDE are diverse, all mutants show similar profiles in distribution of different categories of phenotypic defects (Fig. 2e, f). tmem-132 LOF mutants also exhibited morphological defects in the ADE and PVD but not morphologically less complex AWC neurons (Additional file 1: Fig. S2). Neuronal morphogenesis critically depends on neuronal interactions with glia and epithelia in C. elegans [23, 27, 28]. Importantly, transgenic expression of tmem-132 driven by the osm-6 promoter rescued the morphologic defect of tmem-132 mutants, indicating a causal and cell-autonomous role of TMEM-132 for ensuring normal PDE neuronal morphology (Fig. 2f).
Human TMEM132D interacts with the WRC component NAP1
To begin to understand molecular functions of this conserved protein superfamily, we used yeast-two-hybrid (Y2H) screens to identify protein interactors of human TMEM132D. The predicted intracellular C-terminus of TMEM132D contains cytoplasmic motifs likely related to actin cytoskeletal dynamics [5, 16]. We thus constructed a yeast bait vector expressing its C-terminal domain and used the bait to screen for interactors from a human-cDNA prey library. We also constructed a bait vector that contains the homologous C-terminus of C. elegans tmem-132 and focused on identified screen hits whose homologs can interact with human and C. elegans baits, respectively. From 117 independent cDNA clones isolated and identified by Sanger sequencing (Additional file 2: Table S1), we found that the protein NAP1 encoded by NCKAP1 showed robust interaction with TMEM132D in Y2H assays (Fig. 3a). NAP1 is an integral component of the WAVE regulatory complex that regulates actin nucleation and cytoskeletal changes in the cell through the ARP2/3 complex [16, 18, 29, 30]. We found that GEX-3, the C. elegans ortholog of NAP1, also interacts with the C-terminus of C. elegans TMEM-132 (Fig. 3a).
In addition to NAP1, the WRC also contains three other major proteins SRA1, ABI1/2 and WAVE1/2, with corresponding orthologs gex-2, abi-1 and wve-1 in C. elegans (Fig. 3b) [17, 25, 31]. We verified the biochemical interaction between full-length NAP1 and TMEM132D in mammalian cells by co-immunoprecipitation (CoIP) assays. GFP-tagged NAP1, when expressed in heterologous HEK293 cells with V5 epitope-tagged TMEM132D, was able to pull down TMEM132D in CoIP (Fig. 3c). Another component of WRC, SRA1, is structurally similar to NAP1 and together with NAP1 forms a heterodimeric sub-complex in WRC . GFP-tagged SRA1 also pulled down TMEM132D, although we did not observe apparent association of TMEM132D with other components of WRC. When co-expressed in fully differentiated human neurons derived from induced pluripotent stem cells (iPSC), GFP-tagged TMEM132D markedly co-localized with mCherry-tagged NAP1 (Fig. 3d). Collectively, these genetic, biochemical and cellular imaging results identify NAP1 as a protein interactor of TMEM132D and indicate that such interaction is evolutionarily conserved also for C. elegans counterparts.
The C-terminal domain and the conserved P784 are required for the interactions between TMEM132 and WRC components
We used Y2H and CoIP assays to further define the C-terminal domain of human TMEM132D or C. elegans TMEM-132 that is crucial for interacting with C-termini of NAP1 homologs. To examine interaction between GEX-3 (C. elegans NAP1 homolog) and TMEM-132, we generated transgenic strains in which HA epitope-tagged GEX-3 and mCherry-tagged TMEM-132 Ct are co-induced by heat shock promoters (Fig. 4a). Using the mCherry-Trap CoIP assay, we found that mCherry-tagged TMEM-132 Ct specifically pulled down HA epitope-tagged GEX-3, compared with heat shock-induced mCherry only as control (Fig. 4a). We also confirmed interaction between GEX-3 Ct and TMEM-132 Ct in Y2H assays, in which the most C-terminal 60 a.a. of GEX-3 was sufficient to mediate the interaction (Fig. 4b). In the C-terminus homologous to the WIRS-containing TMEM132D, mutation of a WIRS-like motif attenuated TMEM-132 interaction with GEX-3 (Fig. 4b). Furthermore, we generated mutations to convert the conserved proline 784 to alanine or threonine (to model psychiatric disorder-associated risk allele in humans, see Fig. 1f) in TMEM-132 and found that both mutations abolished the interaction with GEX-3 (Fig. 4c).
Previous studies revealed that the WIRS of diverse transmembrane proteins in mammals mediates binding to an interaction surface of WRC . As TMEM132D contains such a motif at its C-terminus, we performed mutation analysis in Co-IP assays and found that deletion of the entire cytoplasmic portion, deletion of the 120 a.a. C-terminus or mutation of the WIRS-like motif in TMEM132D markedly attenuated its interaction with Nap1 (Fig. 5a). Deletion of the C-terminal 60 a.a. of TMEM132D did not appear to affect the interaction, indicating that additional sites may contribute to the interaction with Nap1. We made similar observations for Sra1 (Fig. 5b), consistent with previous structural findings that Nap1 and Sra1 form an integral heterodimeric sub-complex of WRC . Systematic deletion mutation analysis using Co-IP and Y2H assays underscored the importance of C termini of both C. elegans and human TMEM132 family proteins in interacting with Nap homologs (Fig. 5c). Since canonical WIRS binds to a composite surface formed by Sra and Abi but not Nap , our results suggest that TMEM132 differs from certain canonical WRC ligands, such as PCDH10, in specific interaction with WRC components, consistent with the idea that TMEM132 acts to sequester selective components of WRC rather than to recruit or activate WRC.
WRC acts downstream of TMEM-132 to regulate morphology of the PDE neurons
Since C. elegans TMEM-132 binds to GEX-3 as human TMEM132D binds to NAP1, we next addressed whether TMEM-132 regulates neuronal morphology via WRC in C. elegans. We generated an integrated transgenic reporter with neuronal specific expression of the WRC component ABI-1 fused to GFP. We found that ABI-1::GFP in the nerve ring, along with the ganglia of the head and tail in C. elegans, is weakly fluorescent in wild type animals but strongly up-regulated in tmem-132 mutants (Fig. 6a-c). Close microscopic analysis of ABI-1::GFP specifically in PDE neurons revealed that ABI-1::GFP forms puncta, which decrease in numbers from the larval to adult stages in wild type animals (Fig. 6d). By contrast, numbers of ABI-1::GFP puncta in tmem-132 mutants remain high in both larval and adult stages. We confirmed the increased abundance of ABI-1::GFP in tmem-132 mutants by both whole-animal Western blot and quantitative phenotypic penetrance analysis (Fig. 6a–e). To assess whether abnormally high ABI-1::GFP abundance in tmem-132 mutants is responsible for morphologic defects of PDE neurons, we fed tmem-132 mutants with E. Coli expressing double-stranded RNAi against abi-1 and found that morphologic defects of PDE neurons were largely normalized (Fig. 6f and Additional file 1: Fig. S3). RNAi by feeding produces weaker loss-of-function effects in neurons than by genetic deletion of WRC component-encoding genes, which by itself can cause strong PDE morphologic defects . RNAi against genes encoding other components of WRC, including brk-1 and wve-1, also normalized defects of PDE neurons (Fig. 6g), supporting that abnormally high WRC activity in tmem-132 mutants caused PDE defects. Together, these results indicate that TMEM-132 may orchestrate formation of normal PDE morphology by regulating the neuronal abundance of ABI-1 and restricting WRC activity in the PDE neuron. Interestingly, the CRISPR-mediated P784T substitution in the endogenous TMEM-132 locus caused abnormal neuronal morphology of the PDE neurons in C. elegans (Fig. 2f), supporting the notion that functional roles of TMEM-132 in regulating F-actin and cell morphological changes are mediated by its C-terminal interaction with GEX-3 and thereby interference of WRC and actin nucleation.
Ectopic TMEM132D expression decreases the abundance of WRC components in mammalian cells
We next examined functional consequences of ectopic TMEM132D expression in mammalian cells. While WRC is present in most cell-types in metazoan, expression of endogenous TMEM132D appears to be limited to the nervous system, based on RNA profiling of various mammalian tissues (Additional file 1: Fig. S4). Similarly, we found that expression of C. elegans tmem-132 localizes exclusively in neurons (Fig. 1b–d). We thus established a heterologous HEK293 cell line that stably expresses exogenous V5 epitope-tagged TMEM132D to assess how ectopic expression of TMEM132D affects abundance of WRC components, actin cytoskeleton dynamics and cell motility. Quantified fluorescence signal and Western blot analyses revealed that TMEM132D-expressing cell lines markedly decreased the abundance of ABI1::GFP and WAVE2::GFP while not apparently affecting that of NAP1::GFP or SRA1::GFP, after being transfected as GFP fusion constructs in control and TMEM132D cell lines (Fig. 7a, b). NAP1 and SRA1 are essential for WRC stability and prevent other WRC components from degradation in the cell [18, 32, 33]. Specific down-regulation of ABI1::GFP and WAVE2::GFP but not NAP1 or SRA1::GFP suggests that TMEM132D likely acts to sequester NAP1 and SRA1 in a sub-complex from WRC, leading to disintegration and thus decreased activity of WRC.
To test the hypothesis that TMEM132D inhibits WRC, we used the LifeAct reporter and a wound-recovery assay to examine effects of TMEM132D expression on actin nucleation and cell motility, respectively. LifeAct is a 17-amino-acid polypeptide that labels filamentous actin (F-actin) structures; its fusion with GFP allows visualization and quantification of actin nucleation in eukaryotic cells . We found that expression of epitope-tagged TMEM132D in HEK293T cells markedly reduced LifeAct::GFP signals (Fig. 7c, 7d and Additional file 1: Fig. S5). This was the case even under the condition of serum starvation, which can increase LifeAct::GFP abundance compared with the serum-containing condition (Fig. 7c). Since a constitutive CMV promoter drove the expression of LifeAct::GFP, altered abundance of LifeAct::GFP may reflect endogenous F-actin levels as unbound LifeAct::GFP is unstable and degraded [34, 35]. TMEM132D did not affect overall actin abundance based on Western blot analysis using a pan-actin antibody, indicating specific inhibitory effects of TMEM132D expression on F-actin but not actin monomers. In addition to overall abundance, quantitative cell population-level analysis revealed that TMEM132D also reduced the percentage of cells with strong LifeAct::GFP fluorescence while not affecting the percentage of cells with control GFP fluorescence (Fig. 7d). Furthermore, a wound-recovery assay showed that ectopic TMEM132D-expressing cells exhibited strongly reduced motility during the 24 and 48 h recovery phases after scratching-induced wounding in cultured cells (Fig. 7e, f). Together, these results indicate that ectopic TMEM132D expression may decrease actin nucleation and cell motility, supporting TMEM132D as a NAP1-binding and WRC-inhibiting protein.
Bioinformatic analysis predicted a non-canonical cellular adhesion function for TMEM132 family proteins, connecting extracellular matrix with intracellular actin cytoskeleton . We provide experimental evidence to support this prediction and demonstrate that two members of the TMEM132 protein family from humans and C. elegans regulate cell motility and neuronal morphology, respectively, via inhibition of WRC and actin nucleation. Together, our data support a model in which TMEM132 family proteins via their C-termini bind to and sequester Nap/Sra away from WRC, leading to disintegration and decreased abundance/activity of WRC components such as Abi/Wave, and eventually reduced level of actin nucleation in localized areas of the cell (Fig. 8). Consequently, the high abundance of TMEM132 at local cell surface compartments in wild-type cells likely endows cells, including neurons, with restricted cell motility or morphogenesis, while deficiency of TMEM132 proteins may lead to development of abnormal cell motility or ectopic morphogenesis. The extracellular part of TMEM132 family proteins contain three tandem immunoglobulin domains and a cohesin domain homolog with roles implicated in cellular adhesion . How TMEM132 proteins are regulated under physiological and pathological conditions, potentially through modulation by unidentified extracellular ligands, warrants further investigation.
While major cell morphogenetic events occur during development, both human TMEM132D and C. elegans tmem-132 are also highly expressed in mature neurons of the adult nervous system (Fig. 1b–d and Additional file 1: Fig. S4). Expression of the mouse homolog of TMEM132D is particularly high in the anterior cingulate cortex and claustral neurons, characteristic of long-range connectivity and cellular morphologic complexity [6, 36]. Similarly, PDE neurons in C. elegans at the lateral side of the posterior body send long-range and bifurcated axons to the anterior and posterior nerve ganglia. Neurons form synaptic connections in circuits, in which neuronal activity dynamics can induce local F-actin-dependent changes of neuronal morphology and connectivity [37,38,39]. Such local changes are highly regulated while most surface compartments of mature neurons remain morphologically stable, mechanically resilient and maintained by large repertoires of cell adhesion molecules [40,41,42]. Correspondingly, enrichment of F-actin and actin-nucleating activity are also highly localized and differentially regulated along specific areas of cell processes and neuronal extensions, including dendritic spines, axonal synaptic termini, sensory cilia and microvilli ends [37,38,39, 43,44,45]. We propose that TMEM132 family proteins act to restrict excessive WRC and actin nucleation activities which are spatiotemporally necessary for cellular/neuronal morphological plasticity and maintenance. Emerging evidence suggests that defects in neuronal morphological development and dynamics may underlie aspects of many psychiatric disorders [46,47,48]. Dysfunction or dysregulation of TMEM132D may thus lead to abnormal neuronal structure and dynamics, contributing to heightened risks for depression, anxiety and panic disorders.
Materials and methods
C. elegans strains
C. elegans strains were maintained with standard procedures unless otherwise specified. The N2 Bristol strain was used as the reference wild type. The genetic and transgenic alleles described in this study include Chr. III: tmem-132(dma313), tmem-132(dma317), tmem-132(dma318), tmem-132(dma319), tmem-132(dma348); dmaEx471 [tmem-132p::tmem-132 fl::GFP]; lqIs2 [osm-6p::GFP]; dmaEx452 [osm-6p::abi-1::GFP; unc-54p::mCherry]; dmaIs65 [rab-3p::abi-1::GFP; unc-54p::mCherry]; dmaIs86 [osm-6p::abi-1::GFP; unc-54p::mCherry]; dmaIs91 [osm-6p::tmem-132::GFP]; wyIs592 [ser-2p3::myr-GFP; odr-1p::mCherry]; otIs181 [dat-1p::mCherry + ttx-3p::mCherry]; kyIs140 [str-2p::GFP + lin-15(+)].
Yeast two hybrid assay and screen
The cDNA coding sequence of the C-terminal domain of human TMEM132D was cloned into the pGBKT7 vector and screened with a normalized universal human cDNA library (Clontech, 630,481) in pGADT7 Vector, following instructions in the Matchmaker® Gold Yeast Two-Hybrid System (Clontech, 630,489). Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630,439) and by bait/prey plasmids with re-cloned cDNA.
Co-immunoprecipitation and Western blot
HEK293T cells transfected with mammalian expression plasmids were pelleted by centrifugation, washed once with ice-cold PBS and lysed on ice for 30 min in lysis buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 0.75% NP-40, 0.5% sodium deoxycholate) or Cell Lysis Buffer (Cell Signaling Technology, 9803S) supplemented with protease inhibitor cocktail (Sigma, 11,836,153,001) and phosphatase inhibitor cocktail (Bimake, B15001). Following centrifugation at 12,000 rpm at 4 °C for 15 min, supernatants were recovered. 10% volume of whole cell lysates were collected as input. Lysates were incubated with control (Chromotek) or rabbit IgG beads (Fisher Scientific, 88,802) for preclear at 4 °C for 45 min. Supernatants were recovered and incubated with mCherry-Trap, GFP-Trap (Chromotek) or V5 magnetic beads (MBL International, M167-11) at 4 °C for 2 h. The beads were washed five times by lysis buffer and boiled with SDS sample buffer (Bio-rad, 1,610,747), then separated on 4–15% SDS-PAGE gel (Bio-Rad, 4,561,086) together with input. The proteins were transferred to a nitrocellulose membrane (Bio-Rad, 1,620,167) and detected using the GFP (Santa Cruz Biotechnology, sc-9996) or V5 (EMD Millipore, AB3792) antibody.
Confocal and epifluorescence microscopic imaging
SPE confocal (Leica) and digital automated epifluorescence microscopes (EVOS, Life Technologies) were used to capture fluorescence images. Animals were randomly picked at the same stage and treated with 1 mM Levamisole sodium Azide in M9 solution (31,742-250MG, Sigma-Aldrich), aligned on an 4% agar pad on a slide for imaging. Identical setting and conditions were used to compare experimental groups with control. For quantification of GFP fluorescence animals were outlined and quantified by measuring gray values using the ImageJ software. The data were plotted and analyzed by using GraphPad Prism7.
Mammalian cell culture and wound recovery assay
U2OS and HEK293T cells were cultured in DMEM (Thermo Fisher Scientific, MT-10–013-CV), supplemented with 10% fetal bovine serum (FBS, Gemini Bio-Products, 900–208) and 1% penicillin/streptomycin in a humidified 5% CO2 incubator at 37 °C. Stably expressing TMEM132D::V5 or the control U2OS cells were constructed for wound recovery assay according to the reported protocol . Cells were plated onto the 6-well plate and grew for 16 h to create a confluent monolayer, then cells were washed once by DMEM and cultured in scratch medium (DMEM supplemented with 0.5% FBS and 1% penicillin/streptomycin) for 24 h. The cell monolayer was scraped in a straight line to create a “scratch” with a P200 pipet tip. The debris was removed by washing the cells three times with DMEM medium (0 h). The cells were then cultured for 24 h in scratch medium and imaged at 0 h, 24 h and 48 h. Human excitatory neurons were derived from inducible neurogenin-2(Ngn2) iPSC (i3N iPSCs) as described previously. Briefly, i3N iPSCs were pre-differentiated in KnockOut DMEM/F12 complemented with 2 mg/ml doxycycline, 1 mg/ml mouse Laminin, 10 ng/ml BDNF, 10 ng/ml NT3, 1 × N-2 and 1 × NEAA for 3 days. Media was changed daily with 10 mM Rock inhibitor added only for the first day. After that, the pre-differentiated precursor cells were disassociated with accutase and re-plated into poly-L-lysine coated plates in maturation media which is composed of DMEM/F12: Neurobasal-A/1:1, 2 mg/ml doxycycline, 1 mg/ml mouse Laminin, 10 ng/ml BDNF, 10 ng/ml NT3, 0.5 × N-2, 0.5 × B-27, 0.5 × GlutaMax and 1 × NEAA. Half of the media was replaced every week thereafter without doxycycline supplemented. Human excitatory neurons were infected with lenti-virus (MOI = 1) for 24 h on day 2 of pre-differentiation step. The precursor cells were then re-plated onto coverslips for differentiation into mature neurons and sample replicates were fixed every 3 days for immunohistochemistry.
Data are presented as means ± S.D. with p values calculated by one-way or two-way ANOVA. Data with non-normal distribution, including gene expression and phenotypic penetrance results, were assessed by nonparametric Mann–Whitney and Fisher's exact test, respectively.
Availability of data and materials
Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.
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We thank the Caenorhabditis Genetics Center and the Bargmann, Hobert, Sengupta and Shen laboratories for various C. elegans strains, Dr. Orion Weiner’s laboratory at UCSF for WRC fluorescent reporter constructs, and Dr. Li Gan’s laboratory at the Gladstone Institute and UCSF for iPSC lines.
The work was supported by NIH grants R01GM117461, Pew Scholar Award, Packard Fellowship in Science and Engineering (D.K.M), a fellowship from China Scholarship Council (X.W.) and a China Postdoctoral Foundation fellowship (W.J.). No funding body is involved in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
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C. elegans TMEM-132 is a member of the evolutionarily conserved TMEM132 protein family. Fig. S2. C. elegans TMEM-132 maintains morphologically complex PVD, ADE but not AWC neurons. Fig. S3 Efficacy of RNAi in neurons by feeding from bacteria. Fig. S4. Enrichment of mammalian TMEM132D expression in the brain and claustral neurons. Fig. S5. TMEM132D regulates F-actin abundance.
. List of genes encoding putative TMEM132D interactors identified from yeast-two-hybrid screens.
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Wang, X., Jiang, W., Luo, S. et al. The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex. Mol Brain 14, 54 (2021). https://doi.org/10.1186/s13041-021-00767-w
- Panic disorder
- WAVE regulatory complex
- C. elegans