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Correction to: Reversibility of motor dysfunction in the rat model of NGLY1 deficiency

The Original Article was published on 13 June 2021

Correction to: Mol Brain (2021) 14:91 https://doi.org/10.1186/s13041-021-00806-6

Following publication of the original article [1], the authors would like to correct 2 errors in the "Methods" section.

The first sentence in the "Preparation of cytosolic fraction and enrichment of NGLY1" subsection originally read:

  • For preparation of lysate, 50 mg of rat brain was sliced and resuspended in 500 µl NGLY1 buffer (5 mM Tris–HCl; pH 7.5), 250 mM glycerol, 1 mM Pefabloc™SC (Sigma-Aldrich; 11429868001), and 1 × cOmplete™ protease inhibitor cocktail (Sigma-Aldrich; 11836145001).

The sentence should read (the change has been highlighted in bold typeface):

  • For preparation of lysate, 50 mg of rat brain was sliced and resuspended in 500 µl NGLY1 buffer (100 mM Tris–HCl; pH 7.5, 250 mM sucrose, 1 mM Pefabloc™SC (Sigma-Aldrich; 11429868001), and 1 × cOmplete™ protease inhibitor cocktail (Sigma-Aldrich; 11836145001)).

The second sentence in the "Activity assay of the enriched NGLY1 fraction" section originally read:

  • Briefly, the reaction mixture containing 25 μl of the NGLY1 fraction in a total volume of 30 μl of 10 mM Tris–HCl (pH 7.5), 50 mM sucrose, 1 mM DTT, 1 mM Pefabloc™ SC, and 1 × cOmplete™ protease inhibitor cocktail (EDTA-free) together with 53 pmol of BODIPY- asialoglycopeptide (BODIPY-ASGP) was incubated at 25 °C for 6 h.

The second sentence should read (the change has been highlighted in bold typeface):

  • Briefly, the reaction mixture containing 25 µl of the NGLY1 fraction in a total volume of 30 µl containing 1 mM DTT, 1 mM Pefabloc™ SC, and 1× cOmplete™ protease inhibitor cocktail (EDTA-free) together with 53 pmol of BODIPY-asialoglycopeptide (BODIPY-ASGP) was incubated at 25 °C for 6 h.

These errors do not affect any of the conclusions presented in the article. The original article [1] has been corrected.

Reference

  1. 1.

    Asahina M, Fujinawa R, Hirayama H, Tozawa R, Kajii Y, Suzuki T. Reversibility of motor dysfunction in the rat model of NGLY1 deficiency. Mol Brain. 2021;14:91. https://doi.org/10.1186/s13041-021-00806-6.

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Correspondence to Tadashi Suzuki.

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Asahina, M., Fujinawa, R., Hirayama, H. et al. Correction to: Reversibility of motor dysfunction in the rat model of NGLY1 deficiency. Mol Brain 14, 127 (2021). https://doi.org/10.1186/s13041-021-00825-3

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