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Correction to: Reversibility of motor dysfunction in the rat model of NGLY1 deficiency
Molecular Brain volume 14, Article number: 127 (2021)
Correction to: Mol Brain (2021) 14:91 https://doi.org/10.1186/s13041-021-00806-6
Following publication of the original article [1], the authors would like to correct 2 errors in the "Methods" section.
The first sentence in the "Preparation of cytosolic fraction and enrichment of NGLY1" subsection originally read:
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For preparation of lysate, 50 mg of rat brain was sliced and resuspended in 500 µl NGLY1 buffer (5 mM Tris–HCl; pH 7.5), 250 mM glycerol, 1 mM Pefabloc™SC (Sigma-Aldrich; 11429868001), and 1 × cOmplete™ protease inhibitor cocktail (Sigma-Aldrich; 11836145001).
The sentence should read (the change has been highlighted in bold typeface):
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For preparation of lysate, 50 mg of rat brain was sliced and resuspended in 500 µl NGLY1 buffer (100 mM Tris–HCl; pH 7.5, 250 mM sucrose, 1 mM Pefabloc™SC (Sigma-Aldrich; 11429868001), and 1 × cOmplete™ protease inhibitor cocktail (Sigma-Aldrich; 11836145001)).
The second sentence in the "Activity assay of the enriched NGLY1 fraction" section originally read:
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Briefly, the reaction mixture containing 25 μl of the NGLY1 fraction in a total volume of 30 μl of 10 mM Tris–HCl (pH 7.5), 50 mM sucrose, 1 mM DTT, 1 mM Pefabloc™ SC, and 1 × cOmplete™ protease inhibitor cocktail (EDTA-free) together with 53 pmol of BODIPY- asialoglycopeptide (BODIPY-ASGP) was incubated at 25 °C for 6 h.
The second sentence should read (the change has been highlighted in bold typeface):
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Briefly, the reaction mixture containing 25 µl of the NGLY1 fraction in a total volume of 30 µl containing 1 mM DTT, 1 mM Pefabloc™ SC, and 1× cOmplete™ protease inhibitor cocktail (EDTA-free) together with 53 pmol of BODIPY-asialoglycopeptide (BODIPY-ASGP) was incubated at 25 °C for 6 h.
These errors do not affect any of the conclusions presented in the article. The original article [1] has been corrected.
Reference
Asahina M, Fujinawa R, Hirayama H, Tozawa R, Kajii Y, Suzuki T. Reversibility of motor dysfunction in the rat model of NGLY1 deficiency. Mol Brain. 2021;14:91. https://doi.org/10.1186/s13041-021-00806-6.
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Asahina, M., Fujinawa, R., Hirayama, H. et al. Correction to: Reversibility of motor dysfunction in the rat model of NGLY1 deficiency. Mol Brain 14, 127 (2021). https://doi.org/10.1186/s13041-021-00825-3
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DOI: https://doi.org/10.1186/s13041-021-00825-3