Fig. 4

Increase in anxiety-like and depression-like behaviors in the 9-months-old 6xTg mouse model of AD. a On the light–dark box test, the 6xTg spent significantly less time in the light compartment than WT (n = 12 for WT, 11 for 6xTg; Student’s t-test, *p < 0.05). b On the elevated plus-maze test, the 6xTg spent significantly less time in the open arms than WT (n = 9 for WT, 9 for 6xTg; Student’s t-test, *p < 0.05). Data points from 3 WT mice and 2 6xTg mice were omitted as the animals fell from the behavioral apparatus. c–d In forced swimming test and tail suspension test, the 6xTg displayed increased duration of immobility compared to WT (n = 11 for WT, 11 for 6xTg; Student’s t-test, *p < 0.05, **p < 0.01). In the TST, a data point from 1 WT mouse was omitted as the animal climbed up on its tail. All data are given as means ± SEM