Fig. 2
From: Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP
Long-term memory is normal in 4EHP-CaMKIIα KO mice. A Schematic depicting contextual fear conditioning (CFC) training and LTM test regime. B With weak CFC (0.3 mA, 1 s) training, 4EHP-cKOexc mice (n = 10) show the same freezing behavior as WT (n = 8) 24 h after receiving a foot shock. C 24 h freezing behavior is not altered in 4EHP-cKOexc mice (n = 8) compared to WT (n = 9) with a medium foot shock (0.7 mA, 1 s) training. D Training and testing paradigm for assessing memory in the MWM. Mice were trained to locate a hidden platform in a water maze using spatial cues for 5 days. On the 6th day, the platform was removed, and mice were scored for time spent in each quadrant of the maze. E Learning curve graphed as latency to find platform on training days using a mild training protocol of 1 trial per day (WT n = 9, cKOexc n = 10). F Percent time mice spent in each quadrant. Dashed line at 25% indicates no learning. G Learning curve graphed as latency to find platform on training days using a stronger training protocol of 3 trials per day (WT n = 10, cKOexc n = 16). H Percent time mice spent in each quadrant. Dashed line at 25% indicates no learning. Data are presented as mean ± s.e.m. *p < 0.05; ns, not significant. P value calculated using 2-way ANOVA repeated measures with Bonferroni multiple comparisons test. Sample size is located within the bar graph for each group