- Open Access
Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP
Molecular Brain volume 16, Article number: 9 (2023)
The consolidation of learned information into long-lasting memories requires the strengthening of synaptic connections through de novo protein synthesis. Translation initiation factors play a cardinal role in gating the production of new proteins thereby regulating memory formation. Both positive and negative regulators of translation play a critical role in learning and memory consolidation. The eukaryotic initiation factor 4E (eIF4E) homologous protein (4EHP, encoded by the gene Eif4e2) is a pivotal negative regulator of translation but its role in learning and memory is unknown. To address this gap in knowledge, we generated excitatory (glutamatergic: CaMKIIα-positive) and inhibitory (GABAergic: GAD65-positive) conditional knockout mice for 4EHP, which were analyzed in various behavioral memory tasks. Knockout of 4EHP in Camk2a-expressing neurons (4EHP-cKOexc) did not impact long-term memory in either contextual fear conditioning or Morris water maze tasks. Similarly, long-term contextual fear memory was not altered in Gad2-directed 4EHP knockout mice (4EHP-cKOinh). However, when subjected to a short-term T-maze working memory task, both mouse models exhibited impaired cognition. We therefore tested the hypothesis that de novo protein synthesis plays a direct role in working memory. We discovered that phosphorylation of ribosomal protein S6, a measure of mTORC1 activity, is dramatically reduced in the CA1 hippocampus of 4EHP-cKOexc mice. Consistently, genetic reduction of mTORC1 activity in either excitatory or inhibitory neurons was sufficient to impair working memory. Taken together, these findings indicate that translational control by 4EHP and mTORC1 in both excitatory and inhibitory neurons are necessary for working memory.
Experiments in the 1960’s provided the first evidence for an essential role of protein synthesis in memory formation . Mice trained in a Y-maze shock avoidance task were unable to recall which arm of the Y-maze was linked to the aversive shock 3 days after bilateral intracerebral injection of the protein synthesis inhibitor, puromycin . However, the use of protein synthesis inhibitors provides only limited insight into the biomolecular processes underlying memory. Genetic animal models served as essential tools towards this end. One of the seminal discoveries first documenting that translational control mechanisms govern memories showed that preventing the phosphorylation of eukaryotic initiation factor (eIF)2α, a molecular brake mechanism for protein synthesis, increased general neural translation and enhanced long-term memory (LTM) [2, 3].
mRNA translation is highly regulated at the initiation stage by a heterotrimer protein complex termed eIF4F which interacts directly with the mRNA 5’ cap structure (m7GpppN cap, where N is any nucleotide and m is a methyl group). eIF4F is composed of a cap-binding protein eIF4E, a molecular scaffolding protein eIF4G, and an mRNA helicase eIF4A . Disrupting the interaction between eIF4E and eIF4G using the inhibitor 4EGI-1 prevents LTM . The eIF4E homologous protein (4EHP or eIF4E2) is also a 5’ cap-binding protein, but in contrast to eIF4E it cannot interact with eIF4G and represses translation initiation under most circumstances  (hypoxia being an exception ). 4EHP inhibits translation via the microRNA (miRNA)-induced silencing complex (miRISC) [8, 9]. Despite having a weaker cap-binding affinity than eIF4E , 4EHP can compete with eIF4E for binding the cap upon recruitment by the 4E transporter protein (4E-T) and miRISC . Importantly, translational control via miRNA-induced mRNA silencing plays a critical role in synaptic plasticity and memory . Previously, we identified a critical role for 4EHP in regulating synaptic plasticity (long-term depression) and mediating social behaviors .
Since 4EHP is important for brain function, most likely through miRNA regulation of specific mRNAs important for plasticity-related events at the synapse, we investigated its cell-type-specific activity in learning and memory. To this end, we generated 4EHP conditional knockout (cKO) mice using the Cre-Lox system in excitatory and inhibitory neurons. We did not observe alterations in LTM, but rather an impairment in short-term working memory (WM). We observed reduced ribosomal protein S6 phosphorylation, a prominent readout of the mechanistic target of rapamycin complex 1 (mTORC1) activity, in CA1 hippocampal pyramidal neurons of 4EHP-cKOexc mice. Consistently, conditional deletion of Raptor, the defining component of mTORC1, resulted in impaired WM. Deletion of 4EHP in GABAergic neurons likewise resulted in impaired WM without affecting LTM. The latter effect was likely due to a developmental abnormality, since the total number of GAD67-positive cells was reduced.
Taken together, our findings reveal an important cell-type-specific role of 4EHP in regulating WM possibly through direct modulation of mTORC1 activity, consistent with the known and well-established role of miRNA-mediated regulation of the mTORC1 signaling pathway [13,14,15,16,17], including in the brain .
Materials and methods
Adult (i.e. postnatal day [P] 60–90 ) male mice on Jackson Laboratory C57BL/6J background were used for experiments. Mice were housed by sex and genotype after weaned at P21 in groups of 2–5 animals per cage. Mice were kept at standard room temperature (RT): 20–22 °C on a 12 h light/dark cycle (7:00–19:00 light period) with food and water access ad libitum. Behavioral experiments were conducted in a soundproof room between 8:00 and 16:00. All behavioral apparatuses were cleaned between animals. Mice were handled 2 times (once per day for 2 days) and habituated in the behavioral room for 20 min prior to behavioral testing. See below for detailed behavioral methods. For data acquisition, analysis and manual scoring, the experimenter was blind to mouse genotype which was randomized throughout the day and across days (in the case of multi-day experiments). Animal care, handling, and experiments were performed according to the guidelines of the Canadian Council on Animal Care and approved by the McGill University Animal Care Committee.
Generating conditional knockout (KO) mice
To conditionally delete 4EHP in excitatory neurons, we crossed Eif4e2flx/flx mice  with CaMKIIα-Cre mice (glutamatergic forebrain neurons where Cre recombinase activity has been reported to occur at postnatal day (P) 19 , JAX stock no. 005359, on C57BL/6 background). Eif4e2+/flx:Camk2a-Cre mice were used to breed F2: Eif4e2+/+:Camk2a-Cre (referred to in the text as 4EHP-WT) and Eif4e2flx/flx:Camk2a-Cre (referred to in the text as 4EHP-cKOexc). F3 mice were used for experiments and housed according to genotype. The same breeding scheme was used to generate Eif4e2+/+:Gad2-Cre (referred to in the text as 4EHP-WT) and Eif4e2flx/flx:Gad2-Cre (referred to in the text as 4EHP-cKOinh) using GAD65-Cre mice (GABAergic interneurons, where Cre recombinase activity occurs around embryonic day (e) 15 , JAX stock no. 010802, on C57BL/6 background). Rptorflx/flx:Camk2a-Cre and Rptorflx/flx:Gad2-Cre mice were used as previously characterized . Comparisons were made between + / + and flx/flx mice expressing Cre to normalize for any confounding effects generated by the presence of Cre recombinase alone.
Mouse genotype was determined for each animal using PCR and gel electrophoresis. Eif4e2 gene was amplified using 5’-TCAGAGCAAGAACACTTACAGGACCAAG forward and 5’-GGCCCAGCCTGCCTGGCATTCTAGTGG reverse primers. The PCR product was separated through a 1.5% agarose gel using a 150 V potential difference. WT bands were detected around 700 bp and floxed bands at 850 bp. To detect the presence of Cre (300 bp), the forward 5’-GATTGCTTATAACACCCTGTTACG and reverse 5’-GTAAATCAATCGATGAGTTGCTTCA primers were used.
Soluble protein lysates were prepared by homogenizing brain tissue (from 7 to 9 mice, depending on the experiment) using a pestle grinder in radioimmunoprecipitation assay (RIPA) buffer (R0278, Sigma) on ice containing proteinase (05892970001, Roche) and phosphatase inhibitors (P5726 and P0044, Sigma). Samples were first incubated on ice for 30 min then centrifuged at 16,000 g for 20 min at 4 °C. 25 µg of protein from the supernatant were loaded onto a polyacrylamide gel (final concentration: 12% Acrylamide/Bis Solution, 29:1, 375 mM Tris–HCl pH 8.8, 0.1% SDS, 0.1% TEMED, and 0.1% ammonium persulfate) and separated using a potential difference of 100 V. Protein was then transferred onto a nitrocellulose membrane at 25 V overnight at 4 °C in transfer buffer (25 mM Tris–HCl pH 8.3, 190 mM glycine, and 20% methanol). Membranes were then incubated with 5% bovine serum albumin (BSA) in Tris-Buffered Saline with Tween 20 (TBST, 20 mM Tris–HCl pH 7.5, 150 mM NaCl, 0.1% Tween 20) for 1–2 h at RT to reduce non-specific binding. Membranes were then probed with one of the following primary antibodies at the indicated dilution: EIF4E2 (GTX103977, GeneTex, 1:500), GAPDH (ab9482, Abcam, 1:40 000), diluted in TBST with 5% BSA overnight at 4 °C (or 1 h at RT for GAPDH). Secondary antibody conjugated to horseradish peroxidase (HRP, anti-mouse and anti-rabbit, GE Healthcare) was diluted 1:5000 in TBST with 5% BSA and added to membranes for 1–2 h at RT. Membranes exposed to Blu-Lite UHC Ultra-High Contrast Western blotting film (A8815, MTC Bio) after incubating in enhanced chemiluminescence (Western Lighting® Plus ECL, 0RT2655:0RT2755, Perkin Elmer) for 1 min. Quantification of the band intensity was done using Image J software (NIH).
Immunofluorescence on brain slices
Mice were placed under general isoflurane anesthesia until loss of pain reflex. Mice were then transcardially perfused with filtered ice-cold PBS then ice-cold 4% PFA. Dissected brains were placed in ice-cold 4% PFA overnight at 4 °C for post-fixation and then moved to 30% sucrose in PBS for 3 d at 4 °C for cryoprotection. 20 µm coronal sections were prepared using a cryostat and adhered to glass coverslips (12–550-15, Fisher). Sections were placed in boiling 10 mM sodium citrate buffer, pH 6.0 for 20 min for antigen retrieval after being washed 3 times in PBS for 5 min. Sections were placed in blocking solution (10% BSA and 0.5% Tween 20 in PBS) for 1–2 h at RT. The following primary antibodies were used to probe for: eIF4E2 (sc-100731, Santa Cruz), CaMKIIα (sc-13141), GAD67 (ab213508, Abcam), Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP (5364, Cell Signaling Technology), EMX1 (PA5-35373, Thermo), PVALB (195004, Synaptic System), Somatostatin 28 (ab111912, Abcam), Laminin (L9393, Sigma) diluted 1:100 in blocking solution overnight at 4 °C. Sections were incubated with Alexa-conjugated secondary antibodies (1:300) and Hoechst (1:1000) diluted in blocking buffer for 1–2 h at RT in the dark. Coverslips were mounted with DAKO. Samples were visualized 24 h later with a ZEISS Laser Scanning Microscope 880. Images were quantified using Image-J software. When quantifying fluorescence intensity in inhibitory neurons, the Mean Gray Value (MGV) was scored since this normalizes the area of each cell. For all other quantification, the Integrated Density was scored.
Morris water maze
The Morris water maze (MWM) memory task was performed as previously described . Mice were trained to locate a hidden platform with either 1 trial per day, maximum 60 s per trial (weak) or 3 trials per day, maximum 120 s per trial (strong) over 5 consecutive days in a circular pool 1 m in diameter. Learning was determined manually by timing the latency to locate the hidden (submerged) platform (i.e. escape latency). For probe trials on the following day (day 6), the platform was removed from the maze and the animals were given 60 s to navigate the maze. The percentage of time spent in each quadrant of the maze (quadrant occupancy) was recorded using an automated video tracking system (HVS Image, Buckingham, UK).
The T-maze is a test to measure spatial WM. The T-maze consists of a runway (stem) with a left and right arm choice at the end. The maze is enclosed by short walls so the animal can navigate using cues in the environment. Mice were individually placed at the bottom of the stem. For the training phase, one of the maze arms was closed and the animal was allowed to freely explore the other maze arm for 10 min. After 1 h, the mouse was reintroduced to the maze with exception that the animal could enter the previously closed and unexplored arm. We used the animal’s innate preference for novelty to probe their ability to alternate exploratory behavior of the previously unencountered T-maze arm. Each trial was recorded using an overhead camera and performance was quantified manually. Time spent in the familiar arm and novel arm were scored. From this data we further calculated discrimination index (DI, see equation below).
Novel object recognition
We assessed working memory using a short-term object recognition test. Mice were first placed inside a white box with an open top measuring 50 × 50 × 30 cm. On day 1, mice were habituated to the empty box once for 10 min. After 24 h, mice were placed back in the box with two identical objects and allowed to explore them for 10 min (training). After 1 h, mice were placed back in the box where one of the two objects were replaced with a novel object. Mice freely explored the objects for 10 min (test). Time spent exploring the familiar object and novel object were scored. From this data we derived the discrimination index (DI, see equation above).
Contextual fear conditioning
Mice were placed in a sound-proof box containing an enclosed isolation chamber with an electric grid floor and overhead camera. Mice were recorded for 2 min before receiving a mild foot shock (0.7 mA, 1 s). After 1 min, mice were removed and placed back in their home cage. 1 or 24 h later, mice were placed back in the enclosure (context) and recorded for 4 min. The average percent freezing over 4 min was used as an assessment of LTM.
Statistical analysis was performed on GraphPad Prism 9. An unpaired t-test was used to compare one experimental parameter. Mixed design two-way ANOVA was used to compare two experimental parameters (i.e. genotype as an independent variable and arms in the elevated plus maze test as a repeated measure). Bonferroni test was used for pair-wise post-hoc analysis where there was a significant interaction in the data. A Welch’s corrected t-test was used where the difference in variance between groups was significantly different according to the Levene’s test. Data were expressed as mean ± s.e.m. and p values < 0.05 were considered statistically significant. Details of all statistics used are listed in Additional file 4: Table S1.
Conditional deletion of 4EHP in CaMKIIα-positive excitatory neurons
We previously generated and studied an excitatory neuron-specific 4EHP KO mouse model which displayed impaired sociability, hyperactivity, and synaptic plasticity dysfunction which is reminiscent of autism spectrum disorder (ASD) . We used the Emx1 promoter to drive Cre expression in excitatory neurons since EMX1 is expressed during embryonic development . Deleting 4EHP early in development was chosen to examine developmental effects which are characteristic of ASD. Here, we sought to preclude potential developmental effects of 4EHP on learning and memory by driving Cre expression under the Camk2a promoter, which was originally reported to operate at P19 . More recent work has indicated activity at P14 [26, 27]. To generate excitatory neuron-specific deletion of 4EHP, we crossed Eif4e2flx/flx with mice expressing Cre recombinase under the Camk2a promoter (Fig. 1A). Genotyping confirmed homozygous floxed alleles and the presence of Cre (Fig. 1B). Immunofluorescence imaging of the CA1 hippocampus using antibodies against 4EHP and CaMKII revealed successful cell-type-specific KO of 4EHP directed in excitatory neurons (Fig. 1C). We further confirmed that deletion of 4EHP occurred at P60 (by 22.90 ± 7.28%, Fig. 1D, F) but not at P0 (Fig. 1 D and E) as determined by western blotting. The remaining 4EHP protein in the hippocampus at P60 is possibly due to relatively high levels of expression in other cell types such as glia and inhibitory neurons.
4EHP is dispensable for long-term memory formation
Given the necessity of de novo protein synthesis in LTM formation, we first tested 4EHP-cKOexc mice for long-term contextual fear memory (Fig. 2A). Mice were placed in a context with an electric grid floor and scored for naïve freezing prior to receiving either a weak (0.3 mA) foot shock for 1 s or a medium strength (0.7 mA) foot shock for 1 s. The weak shock was used to prevent a ceiling effect of freezing behavior and allow discrimination of possible memory enhancement in 4EHP-cKOexc mice. LTM was assessed by quantifying freezing behavior 24 h following the foot shock. Under both conditions, LTM was intact in 4EHP-cKOexc mice (Fig. 2B, C). Next, we subjected mice to the MWM spatial navigation learning and memory task (Fig. 2D). Mice were trained to locate a hidden platform over 5 days having been given a training protocol of either 1 trial per day (weak) or 3 trials per day (strong). With a weak training protocol, mice decreased the time needed to find the platform after 5 days (Fig. 2E), but did not show memory retention on the 6th day test where the hidden platform was removed, and mouse quadrant occupancy was scored (Fig. 2F). Given a strong training protocol, 4EHP-cKOexc mice had a significantly increased latency to locate the hidden platform on training day 2 by 16 s but were comparable to WT on days 3 to 5 (Fig. 2G). Both WT and 4EHP-cKOexc mice showed a similar preference for the target quadrant during the test on the following day (Fig. 2H). Importantly, mice significantly decreased the time needed to find the platform during training (Fig. 2E, p = 0.0002; Fig. 2G , p< 0.0001; Additional file 4: Table S1). Together, these data indicate that 4EHP in excitatory neurons does not impact LTM.
Spatial working memory requires 4EHP in excitatory neurons
Working memory (WM), as opposed to LTM, is the short-term storage and manipulation of information for the guidance of decision-making behavior . Deletion of the translational repressor protein 4E-BP2 leads to impairment of WM in mice . This finding suggests that cap-dependent translation controls WM, but the mechanistic details are largely unknown. We tested 4EHP-cKOexc and WT mice in a T-maze spatial WM task (Fig. 3C). Similar to 4E-BP2 KO mice, we observed robust WM impairment in 4EHP-cKOexc mice compared to WT controls (Fig. 3D). Total exploratory behavior, which serves as a control for potential confounding effects of impaired exploratory behavior on memory, was not affected (Fig. 3E). The memory impairments were specific to spatial WM, as short-term (1 h, Fig. 3A) contextual fear memory was not impacted (Fig. 3B). We further validated these findings using an object recognition WM test (Additional file 1: Fig. S1A). Consistent with results from the T-maze, 4EHP-cKOexc demonstrated impaired object recognition WM (Additional file 1: Fig. S1B) without changes in total exploration (Additional file 1: Fig. S1C). From these data, we conclude that 4EHP in excitatory neurons is necessary for intact spatial WM.
Sustained inhibition of translation impairs working memory
We next sought to elucidate how loss of 4EHP disrupts WM based on its known function in mediating miRNA-induced translational silencing . We previously performed an unbiased ribosome profiling study where the translational efficiency (TE) of mRNAs in mouse embryonic fibroblasts (MEFs) lacking 4EHP expression were determined compared to WT . In this study, the ERK1/2 signalling pathway was negatively regulated by 4EHP deletion via translational upregulation of the ERK1/2 phosphatase Dusp6 . However, we did not previously observe changes in p-ERK1/2 in 4EHP KO brain . Given that miRNAs have been shown to regulate the mTOR pathway activity in the brain , we investigated the activity of mTORC1 in the hippocampus of 4EHP-cKOexc mice using immunofluorescence analysis. We observed a 36.40 ± 7.96% reduction in ribosomal protein S6 phosphorylation (which is a readout of mTOR activity) at serine position 240 and 244 [p-S6 (S240/44)] in 4EHP-cKOexc excitatory neurons (Fig. 4A, B), consistent with reduced mTORC1 activity. Furthermore, mice lacking the defining component of the mTORC1 complex (Raptor) in excitatory neurons (Rptorflx/flx:Camk2a-Cre), which results in reduced p-S6 (S240/44) levels (Fig. 4C), have impaired WM (Fig. 4D), without changes in total exploratory behavior (Fig. 4E). Together, these data suggest that WM is impaired because of prolonged attenuation of mTORC1 via loss of 4EHP.
Spatial working memory requires 4EHP and mTORC1 in inhibitory neurons
We next investigated WM in inhibitory neuron-specific 4EHP KO mice (4EHP-cKOinh). We first validated the deletion of 4EHP in GABAergic (GAD67-positive) neurons by immunofluorescence (Fig. 5A) in the prefrontal cortex where 4EHP is primarily expressed in neuronal cell types (Additional file 2: Fig. S2). Like 4EHP-cKOexc mice, 4EHP-cKOinh also demonstrate WM impairment (Fig. 5B) without changes in total exploratory behavior (Fig. 5C). Long-term contextual fear memory was not affected when 4EHP was deleted in inhibitory neurons (Fig. 5D). We explored the possibility that 4EHP regulates mTORC1 activity in inhibitory neurons by quantifying p-S6 in the GAD67-positive neurons. When normalized to the area of the cell, we did not detect a difference in p-S6 fluorescence signal in 4EHP-cKOinh compared to WT (Fig. 5E, F). However, the total number of GAD67-positive cells was significantly reduced (37.82 ± 9.24%) in 4EHP-cKOinh (Fig. 5G). Importantly, mTORC1 activity is still required for WM in inhibitory neurons as Gad2-directed deletion of Raptor (Fig. 5H) impaired WM (Fig. 5I) without affecting total exploratory behavior (Fig. 5J). We conclude that both 4EHP and mTORC1 in inhibitory neurons are required for proper WM.
Rapid de novo protein synthesis in neurons is required for forming long-term memories . Temporally inhibiting eIF4E-dependent translation during consolidation prevents LTM formation . Similarly, translational control via phosphorylation of eIF2α on Ser 51 acts as a critical gatekeeper of LTM as the non-phosphorylatable Ser51Ala mutant mice have a lower threshold for L-LTP and enhanced LTM [2, 3]. Given the necessity of de novo protein synthesis for LTMs and the emerging importance of microRNAs in synaptic plasticity and memory , we investigated the role of 4EHP in LTM. To this end, we subjected 4EHP conditional KO mice to a variety of LTM tests. First, using a contextual fear memory paradigm (CFC), we tested both 4EHP excitatory and inhibitory neuronal KO mice for memory 24 h post-training. In both models, memory retention was comparable to WT controls (Fig. 2B, C and Fig. 5D, respectively). We further assessed 4EHP-cKOexc mice in the MWM where we did not observe any changes to LTM given either a mild or strong training regime (Fig. 2D–H). Given these results, we conclude that 4EHP is not required in excitatory or inhibitory neurons for LTM.
Earlier studies documented WM impairments in mice lacking mRNA translation-repressing proteins, such as 4E-BP2  and PERK (an eIF2α kinase) [32, 33]. Here, we show that a third translational suppressor, 4EHP is required for WM in both excitatory and inhibitory neurons (Figs. 3D and 5B). Since deletion of 4EHP in either cell type abolishes WM, we conclude that the coordinated function of excitatory and inhibitory neurons mediates WM, as opposed to each cell type independently promoting WM.
We previously showed that mice lacking 4EHP in excitatory neurons defined by EMX1 exhibit impaired preference for social novelty in the 3-chamber sociability assay . It can be argued that this impairment is mediated by a WM deficiency, rather than social interaction. However, 4EHP-EMX1 KO mice were also impaired in the direct/reciprocal social assay which does not depend on memory retention of a previous social encounter, unlike the 3-chamber social interaction test. Furthermore, 4EHP-cKOinh mice, which have impaired WM, do not show ASD-like impairments, i.e., social deficits in the 3-chamber assay or exaggerated grooming behavior (Additional file 3: Fig. S3). This raises an intriguing possibility that 4EHP function is not only important for social behavior, but also for cognition in general. This is consistent with findings that executive functions, such as WM, are often impaired in patients with ASD [34,35,36]. Together these studies on 4EHP not only shed light on the mechanistic complexity mediating memory but may offer a potential therapeutic avenue for treating neurodevelopmental disorders such as ASD.
In attempts to understand how 4EHP mediates WM at the cellular and molecular level, we observed a reduction in p-S6 levels in 4EHP null excitatory neurons (Fig. 4A, B), consistent with attenuated activity of mTORC1 signalling. One possible explanation for this finding is that 4EHP represses the translation of mRNAs which encode for proteins that control this signalling pathway. This type of regulation was previously observed in an unbiased ribosome profiling study where the translational efficiency (TE) of mRNAs in mouse embryonic fibroblasts (MEFs) lacking 4EHP expression were determined compared to WT . In this study, TE of the ERK1/2 phosphatase Dusp6 was significantly upregulated in 4EHP KO MEFs which resulted in reduced p-ERK1/2 levels, impairments in cell proliferation, and increased apoptosis . In our previous study, we did not observe changes in p-ERK1/2 in 4EHP KO brain . Another possibility is that reduced mTORC1 activity in cells lacking 4EHP may be a compensatory mechanism to maintain proteostasis. This would be consistent with our previous results showing that global rates of de novo protein synthesis are not changed in the absence of 4EHP in the brain . However, reduction in mTORC1 activity itself can drive WM impairments, as deletion of Raptor alone engendered impaired WM (Fig. 4C, D). This suggests that mTORC1 activity regulates the expression of critical factors involved in WM. In this case, the WM impairments in 4EHP-cKOexc mice are likely a direct consequence of reduced mTORC1 activity by 4EHP-mediated translational control of factors that regulate the mTORC1 pathway.
We further assessed whether mTORC1 activity in 4EHP-cKOinh mice is dysregulated. We did not observe a difference in p-S6 in GAD67-positive neurons in 4EHP-cKOinh compared to controls (Fig. 5E, F). Instead, the total number of GAD67 cells was reduced in 4EHP-cKOinh mice, indicating a developmental abnormality; this is possibly due to the early expression of Cre in Gad2-Cre mice . These results are consistent with the known role of 4EHP in proper mammalian development, as full-body 4EHP KO mice are born underdeveloped and fail to survive . This phenotype may be underlying the observed WM impairment, but further research is required to establish a causal relationship. Deletion of Raptor in inhibitory neurons was sufficient to impair WM (Fig. 5H, I). Together, our results suggest that 4EHP and sustained mTORC1 activity in excitatory or inhibitory neurons is necessary to support WM. Future research is required to determine whether mTORC1 is directly modulated by 4EHP and miRNA-mediated translational control to mediate WM. These mechanistic studies will ideally be carried out in multiple brain regions which are important for WM, such as the hippocampus  and prefrontal cortex .
It is necessary to understand how 4EHP mechanistically regulates mTORC1 activity. The hypothesis that 4EHP controls the translation of kinases and/or phosphatases in the mTORC1 pathway could be studied using an unbiased mRNA translation analysis approach, such as Translating Ribosome Affinity Purification (TRAP)  or RiboTag, according to our previous work . In these methods, a tagged ribosomal protein is expressed through an adeno-associated virus (AAV) under Cre recombinase control. After purifying and sequencing ribosome-bound mRNAs, we can evaluate ribosome occupancy on mRNAs as a measure of active translation across genotypes . This would enable the analysis of the mRNAs in cell types that are translationally regulated by 4EHP.
Availability of data and materials
The data and analysis for the work presented in the current study are available from the corresponding author upon request.
Eukaryotic initiation factor
Eukaryotic initiation factor 4E homologous protein
- CKOexc :
: Conditional knockout in Camk2a-expressing cells
- CKOinh :
: Conditional knockout in Gad2-expressing cells
: MiRNA-induced silencing complex
EIF4E transporter protein
Bovine serum albumin
Tris-Buffered Saline with Tween 20
Mean gray value
Morris water maze
Autism spectrum disorder
Mouse embryonic fibroblast
Contextual fear memory
Translating ribosome affinity purification
Flexner JB, Flexner LB, Stellar E. Memory in mice as affected by intracerebral puromycin. Science. 1963;141:57–9.
Costa-Mattioli M, Gobert D, Stern E, Gamache K, Colina R, Cuello C, Sossin W, Kaufman R, Pelletier J, Rosenblum K, et al. eIF2α phosphorylation bidirectionally regulates the switch from short- to long-term synaptic plasticity and memory. Cell. 2007;129:195–206.
Sharma V, Sood R, Khlaifia A, Eslamizade MJ, Hung T-Y, Lou D, Asgarihafshejani A, Lalzar M, Kiniry SJ, Stokes MP, et al. eIF2α controls memory consolidation via excitatory and somatostatin neurons. Nature. 2020;586:412–6.
Hinnebusch AG, Ivanov IP, Sonenberg N. Translational control by 5′-untranslated regions of eukaryotic mRNAs. Science. 2016;352:1413–6.
Hoeffer CA, Cowansage KK, Arnold EC, Banko JL, Moerke NJ, Rodriguez R, Schmidt EK, Klosi E, Chorev M, Lloyd RE, et al. Inhibition of the interactions between eukaryotic initiation factors 4E and 4G impairs long-term associative memory consolidation but not reconsolidation. Proc Natl Acad Sci. 2011;108:3383–8.
Rom E, Kim HC, Gingras A-C, Marcotrigiano J, Favre D, Olsen H, Burley SK, Sonenberg N. Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein. J Biol Chem. 1998;273:13104–9.
Uniacke J, Holterman CE, Lachance G, Franovic A, Jacob MD, Fabian MR, Payette J, Holcik M, Pause A, Lee S. An oxygen-regulated switch in the protein synthesis machinery. Nature. 2012;486:126–9.
Chapat C, Jafarnejad SM, Matta-Camacho E, Hesketh GG, Gelbart IA, Attig J, Gkogkas CG, Alain T, Stern-Ginossar N, Fabian MR, et al. Cap-binding protein 4EHP effects translation silencing by microRNAs. Proc Natl Acad Sci. 2017;114:5425–30.
Jafarnejad SM, Chapat C, Matta-Camacho E, Gelbart IA, Hesketh GG, Arguello M, Garzia A, Kim S-H, Attig J, Shapiro M, et al. Translational control of ERK signaling through miRNA/4EHP-directed silencing. Elife. 2018;7:e35034.
Zuberek J, Kubacka D, Jablonowska A, Jemielity J, Stepinski J, Sonenberg N, Darzynkiewicz E. Weak binding affinity of human 4EHP for mRNA cap analogs. RNA. 2007;13:691–7.
Wei C-W, Luo T, Zou S-S, Wu A-S. Research progress on the roles of microRNAs in governing synaptic plasticity, learning and memory. Life Sci. 2017;188:118–22.
Wiebe S, Meng XQ, Kim S-H, Zhang X, Lacaille J-C, Aguilar-Valles A, Sonenberg N. The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior. Mol Autism. 2020;11:92.
Zhang Y, Huang B, Wang HY, Chang A, Zheng XFS. Emerging role of MicroRNAs in mTOR signaling. Cell Mol Life Sci. 2017;74:2613–25.
Wang P, Liu XM, Ding L, Zhang XJ, Ma ZL. mTOR signaling-related MicroRNAs and Cancer involvement. J Cancer. 2018;9:667–73.
Rashidi S, Mansouri R, Ali-Hassanzadeh M, Ghani E, Karimazar M, Muro A, Nguewa P, Manzano-Román R. miRNAs in the regulation of mTOR signaling and host immune responses: the case of Leishmania infections. Acta Trop. 2022;231: 106431.
Martin EC, Rhodes LV, Elliott S, Krebs AE, Nephew KP, Flemington EK, Collins-Burow BM, Burow ME. microRNA regulation of mammalian target of rapamycin expression and activity controls estrogen receptor function and RAD001 sensitivity. Mol Cancer. 2014;13:229.
Nazari N, Jafari F, Ghalamfarsa G, Hadinia A, Atapour A, Ahmadi M, Dolati S, Rostamzadeh D. The emerging role of microRNA in regulating the mTOR signaling pathway in immune and inflammatory responses. Immunol Cell Biol. 2021;99:814–32.
Tsujimura K, Irie K, Nakashima H, Egashira Y, Fukao Y, Fujiwara M, Itoh M, Uesaka M, Imamura T, Nakahata Y, et al. miR-199a links MeCP2 with mTOR signaling and its dysregulation leads to rett syndrome phenotypes. Cell Rep. 2015;12:1887–901.
Shoji H, Takao K, Hattori S, Miyakawa T. Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age. Mol Brain. 2016;9:11.
Morita M, Ler LW, Fabian MR, Siddiqui N, Mullin M, Henderson VC, Alain T, Fonseca BD, Karashchuk G, Bennett CF, et al. A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development. Mol Cell Biol. 2012;32:3585–93.
Tsien JZ, Chen DF, Gerber D, Tom C, Mercer EH, Anderson DJ, Mayford M, Kandel ER, Tonegawa S. Subregion- and cell type-restricted gene knockout in mouse brain. Cell. 1996;87:1317–26.
Taniguchi H, He M, Wu P, Kim S, Paik R, Sugino K, Kvitsani D, Fu Y, Lu J, Lin Y, et al. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron. 2011;71:995–1013.
De Gregorio D, Popic J, Enns JP, Inserra A, Skalecka A, Markopoulos A, Posa L, Lopez-Canul M, Qianzi H, Lafferty CK, et al.: Lysergic acid diethylamide (LSD) promotes social behavior through mTORC1 in the excitatory neurotransmission. In: Proceedings of the National Academy of Sciences of the United States of America 2021, 118.
Morris RG, Garrud P, Rawlins JN, O’Keefe J. Place navigation impaired in rats with hippocampal lesions. Nature. 1982;297:681–3.
Gorski JA, Talley T, Qiu M, Puelles L, Rubenstein JLR, Jones KR. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J Neurosci. 2002;22:6309–14.
Hoeffer CA, Tang W, Wong H, Santillan A, Patterson RJ, Martinez LA, Tejada-Simon MV, Paylor R, Hamilton SL, Klann E. Removal of FKBP12 enhances mTOR-Raptor interactions, LTP, memory, and perseverative/repetitive behavior. Neuron. 2008;60:832–45.
Wiebe S, Nagpal A, Truong VT, Park J, Skalecka A, He AJ, Gamache K, Khoutorsky A, Gantois I, Sonenberg N. Inhibitory interneurons mediate autism-associated behaviors via 4E-BP2. Proc Natl Acad Sci USA. 2019;116:18060–7.
Nestler EJ, Hyman SE, Holtzman DM, Malenka RC. Higher cognitive function and behavioral control. Molecular neuropharmacology: a foundation for clinical neuroscience, 3 edn. McGraw Hill. 2014.
Banko JL, Merhav M, Stern E, Sonenberg N, Rosenblum K, Klann E. Behavioral alterations in mice lacking the translation repressor 4E-BP2. Neurobiol Learn Mem. 2007;87:248–56.
Shrestha P, Ayata P, Herrero-Vidal P, Longo F, Gastone A, LeDoux JE, Heintz N, Klann E. Cell-type-specific drug-inducible protein synthesis inhibition demonstrates that memory consolidation requires rapid neuronal translation. Nat Neurosci. 2020;23:281–92.
Shrestha P, Shan Z, Mamcarz M, Ruiz KSA, Zerihoun AT, Juan C-Y, Herrero-Vidal PM, Pelletier J, Heintz N, Klann E. Amygdala inhibitory neurons as loci for translation in emotional memories. Nature. 2020;586(7829):407–11.
Zhu S, Henninger K, McGrath BC, Cavener DR. PERK regulates working memory and protein synthesis-dependent memory flexibility. PLoS ONE. 2016;11: e0162766.
Trinh MA, Kaphzan H, Wek RC, Pierre P, Cavener DR, Klann E. Brain-specific disruption of the eIF2α kinase PERK decreases ATF4 expression and impairs behavioral flexibility. Cell Rep. 2012;1:676–88.
Friedman L, Sterling A. A review of language, executive function, and intervention in autism spectrum disorder. Semin Speech Lang. 2019;40:291–304.
Rabiee A, Vasaghi-Gharamaleki B, Samadi SA, Amiri-Shavaki Y, Alaghband-Rad J. Working memory deficits and its relationship to autism spectrum disorders. Iran J Med Sci. 2020;45:100–9.
Funabiki Y, Shiwa T. Weakness of visual working memory in autism. Autism Res. 2018;11:1245–52.
Leszczynski M. How does hippocampus contribute to working memory processing? Front Hum Neurosci. 2011;5:168.
Lara AH, Wallis JD. The role of prefrontal cortex in working memory: a mini review. Front Syst Neurosci. 2015;9:173.
Nectow AR, Moya MV, Ekstrand MI, Mousa A, McGuire KL, Sferrazza CE, Field BC, Rabinowitz GS, Sawicka K, Liang Y, et al. Rapid molecular profiling of defined cell types using viral TRAP. Cell Rep. 2017;19:655–67.
Ingolia NT. Ribosome profiling: new views of translation, from single codons to genome scale. Nat Rev Genet. 2014;15:205.
We thank Karim Nader (McGill University) for providing access to behavioral testing facilities.
The funding used in the present study was provided by (i) a Canadian Institutes of Health Research (CIHR) foundation grant (FDN-148423), (ii) Howard Hughes Medical Institute Distinguished Researcher (#55007654), (iii) a Brain Canada/FNC grant to N.S., and (iv) the Richard Tomlinson Doctoral Fellowship to S.W. J.C.L. was supported by a CIHR project grant (PJT-153311), a FRQS Research Center grant (Centre Interdisciplinaire de Recherche sur le Cerveau et l’Apprentissage [CIRCA]), and is the recipient of the Canada Research Chair in Cellular and Molecular Neurophysiology (CRC-950-231066).
Ethics approval and consent to participate
All animal procedures and experiments were performed in accordance with the McGill University animal care committee regulations.
Consent for publication
All authors have given their consent for publication.
The authors do not declare any competing/conflicting interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1: Figure S1.
Object recognition working memory. A Mice were assessed for working memory using a novel object recognition task (NOR). B NOR memory is shown as a discrimination index for the novel vs. familiar object where 0% means equal time exploring both objects (WT n = 7, cKOinh n = 10). C Total exploration of objects. Data are presented as mean ± s.e.m. #p < 0.05, calculated using one sample t-test. Sample size is located within the bar graph for each group.
Additional file 2: Figure S2.
4EHP expression pattern in the prefrontal cortex. Analysis of cell-type-specific expression of 4EHP by colocalization with A Empty Spiracles Homeobox 1 (EMX1, defining excitatory neurons), B parvalbumin (PV, defining a subset of inhibitory neurons), C somatostatin (SST, defining another subset of inhibitory neurons), and D laminin (LAMA1, defining endothelial cells) in the prefrontal cortex of WT mice. 4EHP expression is colored in red, the cell type marker in green, and Hoechst-stained nucleus in blue. Arrows indicate a positive signal for the cell type maker. Scale bar represents 20 µm.
Additional file 3: Figure S3.
Inhibitory interneuron-specific deletion of 4EHP does not affect ASD-like behaviors. Assessment of ASD-like behaviors in mice lacking 4EHP expression specifically in inhibitory neurons defined by Gad2. A Total time spent grooming. B Number of grooming bouts. C Sniffing time between either an empty cage (E) or a cage containing a stranger mouse (S1). D Sniffing time between either the previously encountered stranger mouse (S1) or a novel stranger mouse (S2). Data are presented as mean ± s.e.m.; **p < 0.01, ***p < 0.001, ns., not significant; calculated by unpaired t-test or 2-way ANOVA with Bonferroni multiple comparisons test. Sample size is located within bar graphs.
Additional file 4: Table S1.
Summary of statistics
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Wiebe, S., Huang, Z., Ladak, R.J. et al. Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP. Mol Brain 16, 9 (2023). https://doi.org/10.1186/s13041-023-00995-2
- Glutamatergic neurons
- GABAergic neurons
- eIF4E homologous protein (4EHP)
- Mechanistic target of rapamycin complex 1 (mTORC1)