Schizophrenia, amphetamine-induced sensitized state and acute amphetamine exposure all show a common alteration: increased dopamine D2 receptor dimerization
© Wang et al; licensee BioMed Central Ltd. 2010
Received: 13 April 2010
Accepted: 2 September 2010
Published: 2 September 2010
All antipsychotics work via dopamine D2 receptors (D2Rs), suggesting a critical role for D2Rs in psychosis; however, there is little evidence for a change in receptor number or pharmacological nature of D2Rs. Recent data suggest that D2Rs form dimers in-vitro and in-vivo, and we hypothesized that schizophrenia, as well as preclinical models of schizophrenia, would demonstrate altered dimerization of D2Rs, even though the overall number of D2Rs was unaltered.
We measured the expression of D2Rs dimers and monomers in patients with schizophrenia using Western blots, and then in striatal tissue from rats exhibiting the amphetamine-induced sensitized state (AISS). We further examined the interaction between D2Rs and the dopamine transporter (DAT) by co-immunoprecipitation, and measured the expression of dopamine D2High receptors with ligand binding assays in rat striatum slices with or without acute amphetamine pre-treatment.
We observed significantly enhanced expression of D2Rs dimers (277.7 ± 33.6%) and decreased expression of D2Rs monomers in post-mortem striatal tissue of schizophrenia patients. We found that amphetamine facilitated D2Rs dimerization in both the striatum of AISS rats and in rat striatal neurons. Furthermore, amphetamine-induced D2Rs dimerization may be associated with the D2R-DAT protein-protein interaction as an interfering peptide that disrupts the D2R-DAT coupling, blocked amphetamine-induced up-regulation of D2Rs dimerization.
Given the fact that amphetamine induces psychosis and that the AISS rat is a widely accepted animal model of psychosis, our data suggest that D2R dimerization may be important in the pathophysiology of schizophrenia and may be a promising new target for novel antipsychotic drugs.
Schizophrenia is a chronic mental illness, characterized by episodes of psychotic symptoms that usually emerge in early adulthood, last a lifetime, and destroy the mental and interpersonal faculties most valued in human society. The fact that all current antipsychotic drugs exert their effect through the blockade of D2R emphasizes the critical role of hyper-dopaminergic neurotransmission through D2R signaling in the pathophysiology of schizophrenia . Yet, studies are conflicting about the role of D2R in the illness. While postmortem studies have demonstrated an increase in the overall number of D2R, this is confounded by the fact that this increase could be a compensatory response to D2R blockade rather than a diagnostic feature of schizophrenia . On the other hand, PET imaging studies show unequivocal support for a change in D2R . Several recent PET imaging studies show a well-replicated increase in dopamine release in patients with schizophrenia, and even during the prodromal phase . Thus, it remains unclear what, if any, the direct role D2R play in the pathophysiology of schizophrenia.
Most previous studies have measured D2R using receptor radioactive ligand binding assay, PET imaging or Western blot analysis. These techniques provide a good index of overall binding sites, but do not provide any insight into molecular aspects of receptor functions, including their potential configurations with other receptors. Several GPCRs exist in homo- or hetero-dimers to allow multiple signal integrations . A number of studies have focused on the role of dimerization in regulating the function of GPCRs . Indeed, dimerization of GPCRs may alter pharmacological properties and signaling transduction, leading to significant effects on cellular physiology as well as disease pathologies. For example, agonist occupancy of one of the two ligand binding site of the β2-adrenoceptor dimers is sufficient to cause internalization of the dimers. However, both binding sites have to be occupied by the antagonist to prevent agonist-mediated internalization . Furthermore, receptor hetero-dimerization makes it possible to modulate one receptor using ligands targeting the other receptor [7–9]. These unique properties of receptor oligomerization have made them novel targets for the development of novel drugs .
Previous studies indicate that D2R are expressed as monomers and dimers in cell lines and in mammalian brain tissue [11–14]. Furthermore, D2R have also been reported as dimers in a variety of neurological disease such as Alzheimer's, Parkinson's and Huntington's disease [15, 16]. As a result, interest has focused on the potential pathophysiological role of D2R dimerization in disease. Although pharmacological studies have suggested that D2R dimers and monomers have differential affinity for specific dopamine receptor ligands that may subsequently affect dopamine release [17, 18], the functional role of the D2R dimerization and the molecular mechanisms involved are not well understood.
Thus, the purpose of this study was to examine the status of D2R dimers and monomers in schizophrenia. We started with analysis of postmortem striatal sections from schizophrenia patients and observed a significant increase in D2R dimers at the expense of monomers. To examine if this pathology was observed in preclinical models of schizophrenia that involve a hyper-dopaminergic state, we examined the status of D2R dimers in rats exhibiting an amphetamine-induced sensitized state (AISS). A similar increase in D2R dimerization was observed. To further study the molecular basis for this effect (and to link the alteration in D2 receptors to the human observation of alteration in presynaptic dopamine release) we examined the status of D2R as well as their link to the dopamine transporter (DAT) in cultured striatal neurons, with or without acute amphetamine (AMPH) exposure.
Materials and methods
Human postmortem brain tissue
Formalin-fixed paraffin-embedded human postmortem striatum sections (10 μm-thick on glass slides) were donated by the Stanley Foundation Neuropathology Consortium . Subjects were divided into four groups, including bipolar (BD), major depressive disorder (MDD), schizophrenia (SZ), and non-neurological/non-psychiatric controls (n = 15 per group). Subjects were matched for age, gender, postmortem interval (PMI), pH, and mRNA quality. Demographic and medical information such as drug abuse history and psychotropic treatments were provided by the Stanley Foundation Neuropathology Consortium. Diagnoses were retrospectively established by two senior psychiatrists using DSM-IV criteria. All experiments were performed blinded to the diagnosis of each subject.
Adult male Sprague-Dawley rats weighing 250-275 g were procured from Charles River Laboratories, Montreal, Canada. Animals were maintained on a 12-h light/dark cycle and housed two per cage with continuous access to food and water. The animals were allowed to acclimatize to the vivarium for a minimum of 5 days before being used for experimentation. All experimental protocols were approved by the CAMH animal care committee.
Chronic haloperidol treatment
Chronic haloperidol treatment was performed as previously described . Briefly, groups of 12 rats each were randomly assigned to receive one of the following treatments: 0.25 mg/kg/day of haloperidol (McNeil Pharmaceuticals, Spring House, PA) or vehicle via Alzet osmotic mini-pumps (Alzet model 2ML4, Durect Corporation, Cupertino, CA) for a total of 2 weeks, to achieve continuous clinical occupancy.
Amphetamine-induced sensitized state
Amphetamine (Tocris Bioscience, Ellisville, MS) were given three times a week (Monday, Wednesday and Friday) for 5 weeks via intraperitoneal injection (IP). During week 1, amphetamine treated animals received a dose of 1 mg/kg (from salt); with the dose increasing by 1 mg/kg each week so that the dose in the final week was 5 mg/kg. Control animals received saline. All injections were administered in a 1 ml/kg volume.
Acute striatal slices
Acute striatal slices (350 μm-thick) were prepared from Sprague-Dawley rats using a McIlwain tissue chopper (Mickle Laboratory Engineering, Gomshall, United Kingdom). Rat striata were dissected out and left for 5 min in ice-cold artificial cerebrospinal fluid (aCSF) containing 126 mM NaCl, 2.5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, 1.25 mM KH2PO4, 26 mM NaHCO3 and 20 mM glucose, that was bubbled continuously with carbogen (95% O2/5% CO2) to adjust the pH to 7.4. Freshly cut slices were placed in an incubating chamber with carbogenated aCSF and recovered from stress at 37°C for 1 hour. Slices were then treated with 10 μM AMPH for 30 min. Slices were then harvested for Western blot analysis.
Primary cultured striatal neurons
Primary cultures from striatum were prepared from fetal Wistar rats (embryonic day 17-19) on culture dishes as previously described . The cultures were used for experiments 12-15 days after plating.
HEK293T cell culture conditions and transfection
HEK293T cells were cultured in α-MEM (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen) and maintained in incubators at 37°C, 5% CO2. One day before transfection, cells were split onto poly-D-lysine coated plates. For lipofectamine2000 transfections, DAT: D2R cDNA ratio of 1:5 to maximize coexpression. Cells were utilized 2 days post transfection.
Co-immunoprecipitation was performed as previously described [21, 22]. Briefly, solubilized rat striatal extracts (500~700 μg) were incubated in RIPA buffer with primary antibody anti-D2 (Millipore, Billerica, MA) or rabbit IgG (1~2 μg, Sigma-Aldrich, St. Louis, MO) for 4 h at 4°C, followed by the addition of 20 μl of protein A/G agarose (Santa Cruz, Santa Cruz, CA) for 12 h. Pellets were washed, boiled for 5 min in SDS sample buffer and subjected to SDS-PAGE. 20~50 μg of tissue extracted protein was used as a positive control in each experiment. Anti-DAT (Santa Cruz) was applied as the immunoblotting antibody in the following western blot analyses.
Western blot analyses were performed as previously described [21, 22]. Briefly, formalin-fixed paraffin-embedded frozen human postmortem striatal sections from the Stanley Consortium (scraped off the glass slides), rat striatum, HEK293T cells and cultured rat striatal neurons (~2 × 107) were homogenized in RIPA buffer (50 mM Tris-Cl, pH 7.6, 150 mM NaCl, 2 mM EDTA, 1 mM PMSF plus 1% Igepal CA-630, 0.5% sodium deoxycholate, 1% Triton X-100) with a protease inhibitor cocktail (Sigma-Aldrich), and centrifuged at 4°C at 13,000 rpm for 10 min. Supernatant was extracted and protein concentrations were measured (Bio-Rad, Hercules, CA). Protein samples (50 μg) were boiled for 5 min in SDS sample buffer, and subjected to SDS-PAGE. Blots were blocked with 5% non-fat dried milk dissolved in TBST buffer (10 mM Tris, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, washed three times with TBST buffer, incubated with the appropriate primary antibody [anti-D2 (polyclonal) or anti-α-tubulin (monoclonal, Sigma-Aldrich) diluted in 5% milk in TBST] overnight at 4°C. The blots were washed again with TBST buffer three times and then incubated with horseradish peroxidase-conjugated secondary antibody (diluted in 5% milk in TBST; Sigma-Aldrich) for 1.5 h at room temperate. The proteins were visualized with enhanced chemiluminescence reagents (GE Healthcare, Piscataway, NJ).
Inhibition of [3H]-domperidone binding to D2R
The striatal tissue was homogenised (final concentration of 4 mg/ml) in a buffer containing 50 mM Tris-HCl (pH 7.4 at 20°C), 1 mM EDTA, 5 mM KCl, 1.5 mM CaCl2, 4 mM MgCl2 and 120 mM NaCl, using a Teflon-glass homogeniser with the piston rotating at 500 RPM and 10 up-and-down strokes of the glass container. Similar results were obtained with either washed or non-washed homogenates. Although it is known that approximately half the D2R can be lost upon washing the tissue , the homogenates were washed by centrifuging the homogenate at 10,000 × g for 10 min and discarding the supernatant; this procedure was repeated two more times. The final pellet was used.
The D2R in the striatal tissue were measured with [3H]-domperidone (2 nM final concentration; custom synthesized as [phenyl-3H(N)]-domperidone; 41.4 Ci/mmol; Moravek Radiochemicals Inc., Brea, CA) . Each incubation tube (12 × 75 mm, glass) received, in the following order, 0.5 ml buffer, containing a range of dopamine concentrations, with or without a final concentration of 10 μM S-sulpiride (to define nonspecific D2R binding), 0.25 ml [3H]domperidone (generally 1.8 nM as the final concentration in the incubation tube), and 0.25 ml of tissue homogenate. Each concentration of dopamine was tested in duplicate. The tubes, containing a total volume of 1 ml, were incubated for 2 h at room temperature (20°C), after which the incubates were filtered, using a 12-well cell harvester (Titertek, Skatron, Lier, Norway) and buffer-presoaked glass fiber filter mats (Whatman GF/C). After filtering the incubates, the filter mat was rinsed with buffer for 15 s (7.5 ml buffer), and the filters were processed as detailed above. The specific binding of [3H]-domperidone was defined as total binding minus that in the presence of 10 μM S-sulpiride. Independent saturation of D2R, using a range of [3H]-domperidone concentrations, revealed a [3H]-domperidone dissociation constant (Kd) of 0.48 ± 0.08 nM (n = 6) for the rat homogenized striata.
TAT peptides pre-treatment
The acute rat striatal slices were pre-treated with TAT-fused peptides (30 min, 10 μM) before amphetamine treatment. TAT-fused peptides were synthesized by GeneScript. Peptides are rendered cell permeant by fusing to the cell membrane transduction domain of the human immunodeficiency virus type 1 TAT protein (YGRKKRRQRRR), as previously described .
Densitometry and statistical analysis
To quantify the bands obtained via Western blot analysis, we applied ImageJ software based analysis (http://rsb.info.nih.gov/ij/). The area under curve (AUC) of the specific signal was corrected for the AUC of the loading control (e.g. α-tubulin). All values are provided as means ± SEM. For comparisons between two groups, t-test (two-tailed) was performed. For comparisons of more than two groups, one-way ANOVA followed by SNK post-hoc analysis was performed. Unless otherwise noted, significance level was set at 0.05.
Enhanced expression of D2R dimers in postmortem striatal sections from schizophrenia patients
We are aware of the fact that most of the schizophrenia patient samples (13/15) we examined were exposed to antipsychotic treatment ante-mortem. Thus, to rule out the possibility that the observed enhancement of D2R dimerization in the human postmortem schizophrenia striatal sections is the consequence of antipsychotic treatment, we tested the expression of D2R dimerization in striatal brain tissue of rats chronically treated with haloperidol. As shown in Figure 1D-E, haloperidol treatment failed to alter the degree of D2R dimerization. Furthermore, AMPH-induced D2R dimerization was not affected by the haloperidol treatment in rat striatal slices (Figure 1F-G). Taken together, these data suggest that the observed increment of D2R dimers may not be caused by the antipsychotic treatment.
D2R dimerization was up-regulated in the striatal tissue of AISS rats
D2R dimerization may be associated with D2high receptors
Amphetamine facilitates D2R dimerization in primary culture of rat striatal neurons
D2R-DAT protein-protein interaction is involved in amphetamine-induced D2R dimerization
In summary, we have identified a potential role for D2R dimerization in the pathology of schizophrenia. This conclusion is based on our observations of enhanced expression of D2R dimers in postmortem human striatal sections of schizophrenia patients and in the striatum of an animal model of schizophrenia (AISS), as well as the acute amphetamine-induced up-regulation of D2R dimerization. Additionally, we provided evidence that the physical coupling between D2R and DAT may be necessary for this amphetamine-induced up-regulation of D2R dimerization.
It has been long recognized that D2R exists in both monomers and dimers in brain. However, the mechanisms regulating this process as well as the physiological/pathological roles of dimerization remain unknown. Our data provide the first direct evidence that both acute and chronic amphetamine treatments regulate D2R dimerization. Previous studies have shown that amphetamine enhances synaptic dopamine concentrations by the blockade and reversal of DAT-mediated dopamine uptake [31–33]. Thus, an obvious explanation may be that the enhanced synaptic dopamine over-stimulates D2R and increases D2R dimerization. However, our results shown in Figure 4C-D indicate that activation of D2R is not sufficient to induce D2R dimerization. We also observed that acute amphetamine treatment failed to induce D2R dimerization in HEK-293T cells expressing D2R only (Figure 4E-F), suggesting the involvement of additional proteins/receptors in this process. Given that synaptic dopamine activates not only D2R but all dopamine receptor subtypes D1-D5, the observed amphetamine-induced up-regulation of D2R dimerization may also be involved in the activity of other dopamine receptors, such as D1R. Indeed, previous studies have shown a functional interaction between D1R and D2R, and the D1R and D2R may form a protein complex [34–36]. In addition to D1R, recent studies have shown that D2R can also interact with other receptors or channels, such as D3R , Somatostatin sst5 receptor , Adenosine A2A receptor  and Kir3 K+ channel . Several dopamine receptor interacting proteins (DRIPs) have also been identified to be directly and indirectly associated with D2R, including protein 4.1N/B/G , FilaminA , Spinophilin , GIPC , CAPS1 , ZIP , NCS-1 and GRK2 . Future investigations may focus on studying the role of DRIPs as well as other D2R-interacting receptors in the AMPH-mediated D2R dimerization.
In many animal models of psychosis, the proportion of D2R in the high-affinity state is elevated 2- to 9-fold in the striatum of rats known to be supersensitive to dopamine [27, 48, 49]. Our current finding that amphetamine induces an increase in the proportion of D2High receptors and an increase in the D2R dimers in rat tissue suggests that dimerization may be closely related to the dopamine-based hyperactivity elicited by amphetamine.
Previously, we reported that D2R directly interact with DAT, and the D2R-DAT protein complex formation enables D2R to up-regulate DAT-mediated dopamine uptake by recruiting DAT to the plasma membrane . However, the role of D2R-DAT interaction in the regulation of D2R function remains unknown. In the current study, we provided evidence that disruption of the D2R-DAT interaction blocks amphetamine-induced up-regulation of D2R dimerization in transfected HEK293T cells, implicating the potential role of DAT in this process. However, it is worth noting that the AMPH-induced increase in D2R dimers seems to have bands that are more intense than the monomer bands. If the DAT is the only molecule required for this process, it may appear surprising that the DAT in such a small fraction of TH-positive neurons accounts for a large increase in DRD2 dimers in the cultured striatal neurons. Thus, there is a high probability that other additional molecules may be involved in D2R dimerization.
We have shown that D2R dimerization is enhanced in postmortem striatal sections from schizophrenia patients, and that amphetamine, a psychomotor stimulant that can induce psychosis, up-regulates D2R dimerization that in turn may be responsible for the noted dopamine hypersensitivity in schizophrenia. While the current antipsychotic treatments block dopamine D2 receptors, our data show that they do not alter the level of D2 dimers by themselves. Information from Stanley foundation indicated that 13 out of 15 patients in the schizophrenia group have received antipsychotic treatments and exhibited the increment of D2R dimerization. However, 12 out of 15 patients in the bipolar group have also received antipsychotic treatments but did not display an enhanced D2R dimerization. Therefore, a treatment focused at reversing the increased D2 dimerization my be closer to the pathophysiology of schizophrenia and may provide a novel therapeutic target for development of antipsychotics. Previous studies have shown that D2R forms dimers through multiple sites including transmembrane domain 4 . Thus, further studies should be carried out to identify the exact interacting site(s) that are responsible for D2R dimer formation and interfering peptide(s) then can be developed and tested as potential antipsychotic agents in animal models of schizophrenia.
We thank Dr. Albert Wong for proofreading the manuscript, and Boken Lin and Dr. Hongchang Guan for excellent technical assistance. The study is supported by Centre for addiction and Mental Health
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