TWIK-1 contributes to the intrinsic excitability of dentate granule cells in mouse hippocampus
© Yarishkin et al.; licensee BioMed Central Ltd. 2014
Received: 2 July 2014
Accepted: 24 October 2014
Published: 19 November 2014
Two-pore domain K+ (K2P) channels have been shown to modulate neuronal excitability. However, physiological function of TWIK-1, the first identified member of the mammalian K2P channel family, in neuronal cells is largely unknown.
We found that TWIK-1 proteins were expressed and localized mainly in the soma and proximal dendrites of dentate gyrus granule cells (DGGCs) rather than in distal dendrites or mossy fibers. Gene silencing demonstrates that the outwardly rectifying K+ current density was reduced in TWIK-1-deficient granule cells. TWIK-1 deficiency caused a depolarizing shift in the resting membrane potential (RMP) of DGGCs and enhanced their firing rate in response to depolarizing current injections. Through perforant path stimulation, TWIK-1-deficient granule cells showed altered signal input-output properties with larger EPSP amplitude values and increased spiking compared to control DGGCs. In addition, supra-maximal perforant path stimulation evoked a graded burst discharge in 44% of TWIK-1-deficient cells, which implies impairment of EPSP-spike coupling.
These results showed that TWIK-1 is functionally expressed in DGGCs and contributes to the intrinsic excitability of these cells. The TWIK-1 channel is involved in establishing the RMP of DGGCs; it attenuates sub-threshold depolarization of the cells during neuronal activity, and contributes to EPSP-spike coupling in perforant path-to-granule cell synaptic transmission.
KeywordsK2P channel TWIK-1 Intrinsic excitability Dentate gyrus granule cell
Two-pore domain K+ (K2P) channels are major contributors to background potassium conductance in cells and control resting membrane potential (RMP) as well as cellular excitability . These K2P channels are modulated by a large variety of physical and chemical stimuli including temperature, membrane stretch, pH, polyunsaturated fatty acids, hormones and neurotransmitters. Different K2P channel family members have varying functions, with roles in adrenal gland development, thermal and mechanical nociception, and sensitivity to volatile-anesthetics . Of the 15 isoforms in the K2P channel family, K2P1 (TWIK-1; T andem of pore domains in a W eak I nward rectifying K+ channel), was first cloned from human kidney . Due to the low or non-existent functional expression of TWIK-1 in heterologous expression systems , TWIK-1 has long been considered to be non-functional as a plasma membrane channel.
However, and of interest to note, TWIK-1-deficient mice show defects in phosphate transport in the proximal tubule of kidney, as well as deviations in the RMP of pancreatic β cells ,. It has also been recently reported that TWIK-1 is involved in the inward leak Na+ currents during pathological hypokalemia in cardiomyocytes . In addition, TWIK-1 is activated by serotonin in entorhinal cortex stellate cells of the brain, as seen with pharmacological methods  and TWIK-1/TASK-3 heterodimeric channels mediate a halothane response in cerebellum granule cells . We have also recently demonstrated that TWIK-1/TREK-1 heterodimers mediate a passive conductance in astrocytes . These observations strongly suggest about important physiological roles for TWIK-1 channels, although the electrophysiological properties and functional significance of TWIK-1 are not yet fully understood.
The dentate gyrus (DG), sitting between the entorhinal cortex and the CA3 area, is the main gateway to the hippocampus. DG principal neurons, the dentate gyrus granule cells (DGGCs), are capable of effectively blocking or filtering excitatory input from the entorhinal cortex and controlling the amount of excitation that gets through to the hippocampus . The filtering properties of these cells are indispensable for hippocampal-dependent memory processes . DGGCs rely on strong GABA-mediated inhibition and relatively low intrinsic excitability -. As mRNAs of TWIK-1 have been known to be expressed in DGGCs ,, we hypothesized that TWIK-1 may contribute to the electrical properties of DGGCs.
The present study takes advantage of the TWIK-1 gene silencing technique by stereotaxic delivery of adenovirus-carried TWIK-1 shRNA to dentate gyrus, as well as brain slice electrophysiology. Using these methods, we have found that TWIK-1 contributes to the outwardly rectifying potassium currents in DGGCs and determines the intrinsic electrical membrane properties of the cells in brain slices.
TWIK-is expressed in dentate granule cells of mouse hippocampus
TWIK-mediates outwardly rectifying K+ currents in dentate granule cells
Expression of TWIK-1 channels in DGGCs strongly suggests that TWIK-1 might contribute to the electrical properties of these cells. To investigate such a possible role for TWIK-1 in DGGCs, we first examined the effect of knocking down TWIK-1 on the whole-cell currents in these cells. For this purpose we constructed an adenovirus (Ad) carrying a small hairpin-forming interference RNA (shRNA) targeted against mouse TWIK-1. This virus also contained DNA encoding a fluorescent marker, mCherry, which permitted visualization of the location and amount of viral injection. The specificity of the TWIK-1 shRNA has been documented previously .
Knockdown of TWIK-enhances intrinsic excitability of DGGCs
The enhanced firing rate of TWIK-1-deficient DGGCs might be caused solely by the depolarized RMP, which reduces the voltage threshold for excitation to reach the threshold level of firing. In addition, and contributing to the establishment of RMP, K2P channel activity can attenuate the excitatory drive to neurons by providing a shunting potassium conductance, an effect that is strengthened with membrane depolarization ,. Given the significant TWIK-1 shRNA-sensitive component in the whole-cell currents of DGGCs, we suggested that there is a marked shunting effect of TWIK-1 or the TWIK-1 containing channel-mediated conductance on excitatory drive in these cells. To address this possibility, we measured membrane depolarization in response to injected currents in TWIK-1-deficient DGGCs under controlled RMP conditions, in which RMP was maintained at -70mV by constant current injection. The I-V relationship of TWIK-1-deficient DGGCs displays a less prominent outward rectification compared to the I-V of naïve or Sc shRNA-infected cells, evidence of a lack of shunting effect in TWIK-1-deficient DGGCs (Figure3C). To further prove that a lack of TWIK-1-mediated shunting effect may influence the DGGC firing rate, we measured the rheobase current in TWIK-1-deficient DGGCs. Again, the RMP of cells was kept at -70mV by constant current injection into the cell body. A depolarizing current of 2 pA was then injected stepwise until the membrane potential reached the threshold potential level at which a single spike was generated. The rheobase current was significantly smaller in TWIK-1-deficient DGGCs compared to that in naïve and Scrambled control cells (27.6 ± 2.5 pA, 42.7 ± 3.8 pA, 43.8 ± 2.6 pA, respectively; P < 0.05: Figure3D), confirming that depolarization of the RMP of TWIK-1-deficient cells is not the sole cause of their enhanced firing rate, but that a lack of shunting during excitatory post synaptic potentials (EPSPs) also takes place.
These data provide evidence that TWIK-1 contributes to the intrinsic excitability of DGGCs by establishment of the RMP and by providing a potassium conductance, which attenuates membrane depolarization in response to excitatory current injection.
Synaptic response of TWIK-1-deficient dentate granule cells
Our data demonstrate that TWIK-1 is expressed in DGGCs and localized mainly in the cell soma. TWIK-1-deficient GCs had an attenuated outwardly rectifying current density with a reversal potential for currents that was more depolarized than that seen in naïve or Sc shRNA expressing cells. These data show that TWIK-1 contributes to the outwardly rectifying potassium current in DGGCs. The expression of outwardly rectifying leak potassium channels in neuronal cells suggests they contribute to excitability in these cells by establishing the RMP - or by shunting excitation during neuronal activity ,. The shunting effects of K2P channels can result from an increased input from the potassium conductance associated with K2P channel opening, which reduces membrane excitability by attenuating excitatory drive.
The shunting influence of inwardly rectifying currents on the excitability of neuronal cells, particularly, dentate granule cells, was recently demonstrated . The contribution of outwardly rectifying potassium channels to somatic shunting was described in detail for the K v 3 voltage-gated potassium channel in starburst amacrine cells . In these neurons, activation of K v 3 channels provides a voltage-dependent shunt that limits depolarization of the soma to potentials more positive than -20mV, the voltage threshold of the K v 3 channel. Unlike voltage-gated potassium channels, K2P channels do not have an activation threshold. They may therefore provide shunting over the whole physiological range of membrane potentials. We found that TWIK-1-deficient granule cells showed a small but significant depolarizing shift in RMP. In addition, the data we obtained in voltage-clamp experiments with TWIK-1-deficient DGGCs, suggests a marked activity of TWIK-1-mediated current at a membrane potential of -40mV, the spiking threshold level in DGGCs cells. Considering that in a voltage range from the RMP to the spike threshold potential level, the contribution of TWIK-1 channels to the electrical properties of the DGGC plasma membrane is relatively high, we assumed that as a result of this activity in sub-threshold membrane events, an attenuation in the amplitude of membrane depolarization occurs. Indeed, in current-clamp experiments, the relationship between membrane voltage and injected current in TWIK-1-deficient, naïve or Sc shRNA-infected DGGCs revealed a significantly outward rectification in TWIK-1-deficient cells, suggesting that the TWIK-1 current attenuates depolarization of DGGCs in response to current injection.
The influence of TWIK-1-mediated shunting on the excitability of DGGCs was confirmed by measurement of rheobase currents, which were smaller in TWIK-1-deficient cells compared to those in naïve or Scrambled control cells, under the same RMP conditions. A lack of TWIK-1-mediated shunting was also observed in synaptically-evoked depolarizations of the plasma membrane. Under constant RMP conditions, eEPSPs in TWIK-1-deficient cells were of larger amplitude compared to those in naïve and Scrambled control cells, showing that the TWIK-1-mediated conductance attenuates eEPSP amplitude. At the same time, the release probability in perforant path terminals was unaltered, confirming post-synaptic mechanisms for the observed changes in synaptic response. Consistent with a higher intrinsic excitability, TWIK-1-deficient DGGCs had altered excitatory postsynaptic potential-spike coupling, which was demonstrated by an enhanced spiking probability in these cells in response to perforant path stimulation. Of interest to note, is that the DGGC mode of firing, which normally involves the generation of a single spike per one supra-threshold eEPSP event, was converted to a graded burst discharge with increasing stimulus intensity in 44% of TWIK-1-deficient cells. This multiple spike phenomenon could be explained by larger eEPSPs in a fraction of TWIK-1-deficient granule cells that allow membrane potential to be depolarized up to the value of the second spike threshold level after termination of the first action potential. Overall, the combined effect of a depolarized RMP and a lack of shunt in TWIK-1-deficient DGGCs results in the altered signal input-output properties in these cells with increased spike probability and altered EPSP-spike coupling.
Altered input-output properties of TWIK-1-deficient DGGCs, especially changes in firing mode in a sub-population of TWIK-1 knockdown cells, reveal an important role for TWIK-1 in the filtering properties of DGGCs and hence the physiological function of DG as a gateway to the hippocampus. Though the precise regulatory mechanisms of TWIK-1 channel activity are not well established, we speculate that modulation of the intrinsic excitability of DGGCs by up- or down-regulation of TWIK-1 channel activity may occur either in normal or pathological states that might affect the filtering properties of DGGCs and hence influence information processing from entorhinal cortex to hippocampus.
The outwardly rectifying properties of TWIK-1-mediated currents seen in DGGCs was a somewhat surprising finding as currents mediated by TWIK-1 expressed heterologously in Xenopus laevis oocyte have been characterized as weakly inwardly rectifying ,. The reason for the observed discrepancy between the biophysical characteristics of TWIK-1 currents in Xenopus laevis oocytes and our own data is not clear for now. However, it has been suggested that for its functional expression , TWIK-1 might require cellular factors that have yet to be discovered. These may regulate the surface expression of TWIK-1 or its channel properties. Worth noting, is that biophysical properties of the deSUMOylated TWIK-1 current were described to be outwardly rectifying ,. Thus, the properties of cloned TWIK-1 might differ from those of TWIK-1 in its native environment. As well as this, recent publications provide evidence for heteromerization between TWIK-1 and other K2P channels, such as TASK-3 and TREK-1. Heteromerization between TWIK-1 and TASK-3 results in formation of a channel with outwardly rectifying properties , while TWIK-1/TREK-1 heterodimer-mediated currents demonstrate a linear I-V relationship . It requires further in-depth investigations what is the molecular mechanisms that confers TWIK-1 with a functional channel properties in DG GCs.
The physiological function of TWIK-1, the first identified member of the K2P channel family, in neuronal cells is largely unknown. We demonstrate, for the first time, a functional expression of TWIK-1 in DGGCs in mouse hippocampus. TWIK-1 mediates outwardly rectifying currents and is involved in establishing the RMP of DGGCs. TWIK-1 attenuates sub-threshold depolarization of the cells during neuronal activity, and contributes to EPSP-spike coupling in perforant path-to-granule cell synaptic transmission. Taken together, these results clearly showed that TWIK-1 has a pivotal role in the electrical properties of DGGCs.
Male C57BL/6 mice, aged 7-8 weeks old, were used for all experiments. Animal care and handling were performed according to instructional guidelines of the Korea Institute of Science and Technology (Seoul, Korea).
Construction of the recombinant adenovirus vector
pSicoR-Scrambled and pSicoR-TWIK-1 shRNA plasmid  were cloned into pDONRTM 207 vectors (Invitrogen), and confirmed by DNA sequencing. An LR recombination reaction was performed between the cloned plasmid and pAd/CMV/V5-DESTTM destination vector, using LR ClonaseTM enzyme mix. Adenoviral vectors carrying each shRNA were then linearized by PacI digestion and used to transfect 293A cells using Lipofectamine® 2000 reagent. The viral titer was determined in a 96-well plate according to the manufacturer's instructions.
Stereotaxic virus injections
Virus injections were performed on deeply anesthetized mice of 7 weeks old, which were placed in a stereotaxic frame (Kopf Instruments). Anesthesia was induced with Avertin® (2,2,2-tribromethanol in 2-methyl-2-butanol) via intraperitoneal injection. Briefly, the scalp was opened and two holes were drilled in the skull (-2.0 mM AP, ±1.3 mM ML from bregma). Ad-shTWIK-1-mCherry or Ad-Scrambled-mCherry (2μl per side) was injected bilaterally into the dentate gyrus (1.6 mM DV from the dura) through a glass microdispenser (VWR, USA) with a syringe pump (KD Scientific, USA) that infused the virus at a speed of 0.2μl/min. The microdispenser was left in place for 2 min before and 2 min after the injection.
Fluorescence immunohistochemistry and image collection
Mouse tissues were obtained following intracardiac perfusion with saline and 4% paraformaldehyde solution and 20μm-thick frozen tissue sections were prepared using a cryostat. Sections were mounted onto slides and permeabilized with 0.5% Triton X-100 in PBS for 20 min at room temperature (RT, 20-22 °C) followed by blocking with 10% donkey serum and 0.1% Triton X-100 in PBS for 1 hr at RT. Tissues were incubated overnight with primary antibodies, such as rabbit anti-TWIK-1 polyclonal antibody (1:100; Alomone, Jerusalem, Israel) or chicken anti-MAP2 polyclonal antibody (1:1000; Abcam, Cambridge, MA), at 4°C. For detection, suitable fluorescence (Alexa fluor)-tagged secondary antibodies (Molecular Probes, Eugene, OR) were used and tissues were counterstained with DAPI.
The mouse brain was isolated and the DG and other hippocampal regions (CA) were dissected out. The obtained DG and CA tissues were lysed with radioimmunoprecipitation assay buffer and processed for Western Blotting using rabbit anti-TWIK-1 antibody (1:1000; Abcam, Cambridge, MA) and mouse anti-GAPDH (1:5000; Abcam, Cambridge, MA). Signals were detected by enhanced chemiluminescence (GE Healthcare, UK) following probing with the appropriate HRP-conjugated secondary antibodies (Jackson Laboratory, Bar Harbor, ME). Each experiment was performed with samples from three independent groups. Signal intensity was quantified using ImageJ software.
Brain slice preparation and electrophysiology
Brain slice electrophysiology was performed at 6-7th day after virus injections. Mice were decapitated after induction of anesthesia by ether. Mouse brains were rapidly removed and placed into ice-cold modified artificial cerebrospinal fluid (ACSF) containing (in mM) 130 NaCl, 2.5 KCl, 1.25 KH2PO4, 3.0 MgCl2, 1.0 CaCl2, 26 NaHCO3, and 10.0 D-glucose. For electrophysiological experiments 400μm thick horizontal sections containing hippocampi were prepared using a vibratome (Linear slicer DSK, Japan) in ice-cold oxygenated ACSF. After resting for 1-2 h in ACSF of the same composition, at RT, slices were placed onto the recording chamber and experiments were performed in ACSF that contained (in mM): 130 NaCl, 2.5 KCl, 1.25 KH2PO4, 1.5 MgCl2, 1.5 CaCl2, 26 NaHCO3, and 10.0 D-glucose. Hippocampal slices were visualized with an Olympus BX51WI microscope equipped with epifluorescence. mCherry-positive cells were selected for whole-cell patch recording. Patch pipettes were made from borosilicate glass and had a resistance of 7-10 M . Pipette solution contained (in mM): 120 K-gluconate, 10 KCl, 1 MgCl2, 0.5 EGTA, 40 HEPES (pH adjusted to 7.2 with KOH). In voltage-clamp experiments the holding membrane potential was set to -70mV. Series and input resistances were monitored throughout the experiment using a -5mV pulse. Recordings were considered stable when both the series and input resistance, and the RMP did not change >20%. Recordings were filtered at 2 kHz and digitized at 10 kHz. In current-clamp experiments, currents were injected stepwise, in 25 pA steps. Step duration was 1.2 s. Granule cells were identified by morphological and physiological criteria such as a highly negative RMP and the absence of a voltage "sag" in response to hyperpolarizing current injection. Recordings were made from granule cells located in the middle or the outer third of the dentate granule cell layer. Granule cells were only included in the study if they had a RMP more negative than -60mV and if the amplitude of the action potential was higher than 50mV. In voltage-clamp experiments whole-cell currents were elicited by ramp pulses descending from 40mV to -150mV for 1'sec. The descending epoch in the protocol was preceded with a voltage step to 40mV for 500 ms from the holding potential of -70mV. Synaptic responses were evoked by 0.1 Hz stimulation of perforant path fibers (100μsec duration; 100-1000μA intensity) via a constant current isolation unit (WPI, USA) connected to a concentric electrode (FHS Inc, USA). Perforant path fibers were stimulated by placing the stimulation electrode in the outer or the middle third of the molecular layer of the dentate. Spiking probability was calculated as the ratio of a number of successful (spike-generating) stimulations to the total number of stimulations (10 stimulations delivered at 0.1Hz). The stimulus intensity was in the range of 100 to 400μA, applied in 50μA increments. Paired-pulses were applied with inter-pulse intervals ranging from 20 ms through 2000 ms, in 20 ms increments. All experiments were performed in the presence of GABAA and GABAB antagonists bicuculline (10μM) and CGP 55845 (10μM), respectively. When the response of membrane potential to stepwise current injections was examined, the RMP was maintained at -70 mV. ACSF solution contained 50 M D-AP5, 10 M CNQX, 10 M bicuculline, 10 M CGP 55845, 2 mM tetraethyl ammonium chloride, and 0.5 mM NiCl2; pipette solution contained 5 mM QX-314 to block the action potentials firing. The recordings for representative traces of rheobase current measurements were performed in ASCF containing 50 M D-AP5, 10 M CNQX, 10 M bicuculline, and 10 M CGP 55845. Data were collected with a MultiClamp 700B amplifier (Molecular Devices) using Clampex 10 data acquisition software (Molecular Devices) and were digitized with Digidata 1322A (Molecular Devices). All experiments were performed at RT.
Bicuculline methobromide, CGP 55845 hydrochloride, D-AP5, CNQX, and QX-314 were purchased from Tocris. Other reagents were purchased from Sigma or from Calbiochem.
Numerical data are presented as means ± S.E.M. Error bars in graphs denote the standard error of the mean. The statistical significance of data was assessed by Student's unpaired or paired t-test. The significance level is shown with asterisks (*P < 0.05 or **P < 0.01).
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) (NRF-2014R1A2A1A01007039) for JYP, and supported by the KIST institutional program (Project No. 2E25170) for EMH.
- Enyedi P, Czirj'k G: Molecular background of leak K+ currents: two-pore domain potassium channels. Physiol Rev. 2010, 90: 559-605. 10.1152/physrev.00029.2009.PubMedView ArticleGoogle Scholar
- Lesage F, Guillemare E, Fink M, Duprat F, Lazdunski M, Romey G, Barhanin J: TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure. EMBO J. 1996, 15: 1004-1011.PubMedPubMed CentralGoogle Scholar
- Rajan S, Plant LD, Rabin ML, Butler MH, Goldstein S a N: Sumoylation silences the plasma membrane leak K+ channel K2P1. Cell. 2005, 121: 37-47. 10.1016/j.cell.2005.01.019.PubMedView ArticleGoogle Scholar
- Nie X, Arrighi I, Kaissling B, Pfaff I, Mann J, Barhanin J, Vallon V: Expression and insights on function of potassium channel TWIK-1 in mouse kidney. Pflugers Arch. 2005, 451: 479-488. 10.1007/s00424-005-1480-9.PubMedView ArticleGoogle Scholar
- Chatelain FC, Bichet D, Douguet D, Feliciangeli S, Bendahhou S, Reichold M, Warth R, Barhanin J, Lesage F: TWIK1, a unique background channel with variable ion selectivity. Proc Natl Acad Sci U S A. 2012, 109: 5499-5504. 10.1073/pnas.1201132109.PubMedPubMed CentralView ArticleGoogle Scholar
- Ma L, Zhang X, Chen H: TWIK-1 two-pore domain potassium channels change ion selectivity and conduct inward leak sodium currents in hypokalemia. Sci Signal. 2011, 4: ra37-10.1126/scisignal.2001726.PubMedView ArticleGoogle Scholar
- Deng P, Poudel SKS, Rojanathammanee L, Porter JE, Lei S: Serotonin inhibits neuronal excitability by activating two-pore domain K+ channels in the entorhinal cortex. Mol Pharmacol. 2007, 72: 208-218. 10.1124/mol.107.034389.PubMedView ArticleGoogle Scholar
- Plant LD, Zuniga L, Araki D, Marks JD, Goldstein S a N: SUMOylation silences heterodimeric TASK potassium channels containing K2P1 subunits in cerebellar granule neurons. Sci Signal. 2012, 5: ra84-PubMedView ArticleGoogle Scholar
- Hwang EM, Kim E, Yarishkin O, Woo DH, Han K-S, Park N, Bae Y, Woo J, Kim D, Park M, Lee CJ, Park J-Y: A disulphide-linked heterodimer of TWIK-1 and TREK-1 mediates passive conductance in astrocytes. Nat Commun. 2014, 5: 3227-PubMedGoogle Scholar
- Hsu D: The dentate gyrus as a filter or gate: a look back and a look ahead. Prog Brain Res. 2007, 163: 601-613. 10.1016/S0079-6123(07)63032-5.PubMedView ArticleGoogle Scholar
- Jinde S, Zsiros V, Jiang Z, Nakao K, Pickel J, Kohno K, Belforte JE, Nakazawa K: Hilar mossy cell degeneration causes transient dentate granule cell hyperexcitability and impaired pattern separation. Neuron. 2012, 76: 1189-1200. 10.1016/j.neuron.2012.10.036.PubMedPubMed CentralView ArticleGoogle Scholar
- Staley KJ, Otis TS, Mody I: Membrane properties of dentate gyrus granule cells: comparison of sharp microelectrode and whole-cell recordings. J Neurophysiol. 1992, 67: 1346-1358.PubMedGoogle Scholar
- Williamson A, Spencer DD, Shepherd GM: Comparison between the membrane and synaptic properties of human and rodent dentate granule cells. Brain Res. 1993, 622: 194-202. 10.1016/0006-8993(93)90819-9.PubMedView ArticleGoogle Scholar
- Cohen AS, Lin DD, Quirk GL, Coulter DA: Dentate granule cell GABA(A) receptors in epileptic hippocampus: enhanced synaptic efficacy and altered pharmacology. Eur J Neurosci. 2003, 17: 1607-1616. 10.1046/j.1460-9568.2003.02597.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Mody I: Aspects of the homeostaic plasticity of GABAA receptor-mediated inhibition. J Physiol. 2005, 562 (Pt 1): 37-46. 10.1113/jphysiol.2004.077362.PubMedPubMed CentralView ArticleGoogle Scholar
- Talley EM, Solorzano G, Lei Q, Kim D, Bayliss D a: Cns distribution of members of the two-pore-domain (KCNK) potassium channel family. J Neurosci. 2001, 21: 7491-7505.PubMedGoogle Scholar
- Aller MI, Wisden W: Changes in expression of some two-pore domain potassium channel genes (KCNK) in selected brain regions of developing mice. Neuroscience. 2008, 151: 1154-1172. 10.1016/j.neuroscience.2007.12.011.PubMedView ArticleGoogle Scholar
- Riazanski V, Becker A, Chen J, Sochivko D, Lie A, Wiestler OD, Elger CE, Beck H: Functional and molecular analysis of transient voltage-dependent K + currents in rat hippocampal granule cells. J Physiol. 2001, 537 (Pt 2): 391-406. 10.1111/j.1469-7793.2001.00391.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Cuppini C, Ambrogini P, Lattanzi D, Ciuffoli S, Cuppini R: FGF2 modulates the voltage-dependent K+ current and changes excitability of rat dentate gyrus granule cells. Neurosci Lett. 2009, 462: 203-206. 10.1016/j.neulet.2009.07.029.PubMedView ArticleGoogle Scholar
- Esp'sito MS MLA, Lombardi G, Schinder AF: Reliable activation of immature neurons in the adult hippocampus. PLoS One. 2009, 4: e5320-10.1371/journal.pone.0005320.View ArticleGoogle Scholar
- Young CC, Stegen M, Bernard R, M'ller M, Bischofberger J, Veh RW, Haas CA, Wolfart J: Upregulation of inward rectifier K+ (Kir2) channels in dentate gyrus granule cells in temporal lobe epilepsy. J Physiol. 2009, 587 (Pt 17): 4213-4233. 10.1113/jphysiol.2009.170746.PubMedPubMed CentralView ArticleGoogle Scholar
- Stegen M, Young CC, Haas CA, Zentner J, Wolfart J: Increased leak conductance in dentate gyrus granule cells of temporal lobe epilepsy patients with Ammon's horn sclerosis. Epilepsia. 2009, 50: 646-653. 10.1111/j.1528-1167.2009.02025.x.PubMedView ArticleGoogle Scholar
- Goldstein SA, Bockenhauer D, O'Kelly I, Zilberberg N: Potassium leak channels and the KCNK family of two-P-domain subunits. Nat Rev Neurosci. 2001, 2: 175-184. 10.1038/35058574.PubMedView ArticleGoogle Scholar
- Patel AJ, Honor' E: Properties and modulation of mammalian 2P domain K+ channels. Trends Neurosci. 2001, 24: 339-346. 10.1016/S0166-2236(00)01810-5.PubMedView ArticleGoogle Scholar
- Brickley SG, Revilla V, Cull-Candy SG, Wisden W, Farrant M: Adaptive regulation of neuronal excitability by a voltage-independent potassium conductance. Nature. 2001, 409: 88-92. 10.1038/35051086.PubMedView ArticleGoogle Scholar
- Bean BP: The action potential in mammalian central neurons. Nat Rev Neurosci. 2007, 8: 451-465. 10.1038/nrn2148.PubMedView ArticleGoogle Scholar
- Brickley SG, Aller MI, Sandu C, Veale EL, Alder FG, Sambi H, Mathie A, Wisden W: TASK-3 two-pore domain potassium channels enable sustained high-frequency firing in cerebellar granule neurons. J Neurosci. 2007, 27: 9329-9340. 10.1523/JNEUROSCI.1427-07.2007.PubMedView ArticleGoogle Scholar
- Stegen M, Kirchheim F, Hanuschkin A, Staszewski O, Veh RW, Wolfart J: Adaptive intrinsic plasticity in human dentate gyrus granule cells during temporal lobe epilepsy. Cereb Cortex. 2012, 22: 2087-2101. 10.1093/cercor/bhr294.PubMedView ArticleGoogle Scholar
- Ozaita A, Petit-Jacques J, V'lgyi B, Ho CS, Joho RH, Bloomfield SA, Rudy B: A unique role for Kv3 voltage-gated potassium channels in starburst amacrine cell signaling in mouse retina. J Neurosci. 2004, 24: 7335-7343. 10.1523/JNEUROSCI.1275-04.2004.PubMedView ArticleGoogle Scholar
- Lesage F, Lauritzen I, Duprat F, Reyes R, Fink M, Heurteaux C, Lazdunski M: The structure, function and distribution of the mouse TWIK-1'K+ channel. FEBS Lett. 1997, 402: 28-32. 10.1016/S0014-5793(96)01491-3.PubMedView ArticleGoogle Scholar
- Plant LD, Rajan S, Goldstein SAN: K2P channels and their protein partners. Curr Opin Neurobiol. 2005, 15: 326-333. 10.1016/j.conb.2005.05.008.PubMedView ArticleGoogle Scholar
- Plant LD, Dementieva IS, Kollewe A, Olikara S, Marks JD, Goldstein SAN: One SUMO is sufficient to silence the dimeric potassium channel K2P1. Proc Natl Acad Sci U S A. 2010, 107: 10743-10748. 10.1073/pnas.1004712107.PubMedPubMed CentralView ArticleGoogle Scholar
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