c-Fos expression in the paternal mouse brain induced by communicative interaction with maternal mates
© Zhong et al.; licensee BioMed Central Ltd. 2014
Received: 27 March 2014
Accepted: 25 August 2014
Published: 11 September 2014
Appropriate parental care by fathers greatly facilitates health in human family life. Much less is known from animal studies regarding the factors and neural circuitry that affect paternal behavior compared with those affecting maternal behavior. We recently reported that ICR mouse sires displayed maternal-like retrieval behavior when they were separated from pups and caged with their mates (co-housing) because the sires receive communicative interactions via ultrasonic and pheromone signals from the dams. We investigated the brain structures involved in regulating this activity by quantifying c-Fos-immunoreactive cells as neuronal activation markers in the neural pathway of male parental behavior.
c-Fos expression in the medial preoptic area (mPOA) was significantly higher in sires that exhibited retrieval behavior (retrievers) than those with no such behavior (non-retrievers). Identical increased expression was found in the mPOA region in the retrievers stimulated by ultrasonic vocalizations or pheromones from their mates. Such increases in expression were not observed in the ventral tegmental area (VTA), nucleus accumbens (NAcc) or ventral palladium (VP). On the following day that we identified the families of the retrievers or non-retrievers, c-Fos expression in neuronal subsets in the mPOA, VTA, NAcc and VP was much higher in the retriever sires when they isolated together with their mates in new cages. This difference was not observed in the singly isolated retriever sires in new cages. The non-retriever sires did not display expression changes in the four brain regions that were assessed.
The mPOA neurons appeared to be activated by direct communicative interactions with mate dams, including ultrasonic vocalizations and pheromones. The mPOA-VTA-NAcc-VP neural circuit appears to be involved in paternal retrieval behavior.
Parental care is critically important for the survival and proper development of young mammals . Numerous studies on the neural circuitry of female parental behavior have shown that medial preoptic area (mPOA) is important for the control of maternal behavior in rodents . Far fewer reports investigating paternal behavior have revealed that the mPOA is responsible for paternal behavior . Several investigations have explored the roles of the ventral tegmental area (VTA), the nucleus accumbens (NAcc) and the ventral palladium (VP) in the regulation of parental behavior ,,,,,. Briefly, mPOA neurons activate VTA dopaminergic neurons, which lead to activation of the D1 receptors in NAcc. When NAcc GABAergic neurons are activated, they relieve the tonic inhibition of VP neurons. Disinhibiting VP neurons may actuate the motor activities of maternal parental behavior ,.
We developed a co-housing paradigm to induce paternal behavior . When mouse sires of the ICR strain are continuously housed with their pair mates and pups for three to five days after parturition, the sires exhibit features of normal maternal care (crouching, licking and pup retrieval). When separated from their pups for 10 min and co-housed with their mates or kept in a vacated family cage during the separation, the sires exhibited retrieval behavior when reunited with their pups . The sires housed alone in a new cage during the separation period for 3 30 min did not exhibit retrieval behavior upon reunion with their pups. The acquisition of retrieval behavior by the co-housed male parents required at least 5 10 min of separation, during which it is hypothesized that communicative interaction occurs between the couple .
We revealed that male parental care behavior was triggered by olfactory and specialized, 38-kHz ultrasonic auditory signals (USV, ultrasonic vocalization) from the dam during the interactive co-housing period . The neural circuit responsible for this particular paternal behavior in the ICR strain is less clear. We recently demonstrated that scopolamine, a muscarinic receptor antagonist and muscarinic receptor-coupled KCNQ/Kv7 potassium channel opener, inhibits paternal behavior . We demonstrated that paternal behaviors, including pup retrieval, grooming, crouching or huddling, were improved in CD38 knockout sires treated with oxytocin and/or with local re-expression of CD38 in the NAcc compared with the parental behaviors in untreated CD38 knockout sires . These results suggest that the central cholinergic pathway and NAcc neurons mediate this paternal behavior. The other brain regions that are involved remain to be elucidated.
Experiments on day 1
Figure 1 illustrates the experimental paradigm of the different housing conditions of one family composed of a sire, dam and their biological pups and of the separation conditions for the induction of paternal behavior during a two-day experiment. In the first set of experiments, we performed pup-directed retrieval experiments (S1 - S4). We examined and compared the sires exhibiting retrieval (retrievers, S4-2) and those not exhibiting retrieval (S4-1, non-retrievers) during a 10-min observation period, after separation of the mated pair for 10 min (S3). The sires were sacrificed after an additional 10 min (a total duration of 30 min from exposure to the first social stimulation), allowing for c-Fos protein induction to a level that visible enough to for comparison, because a previous study  shows that c-Fos protein is expressed at 30 min but reaches peak at 90 min. We selected this time window to detect the effect due only to the co-housed condition, thus minimizing the influence of other factors, and a more preferred technique of an immunohistochemistry method to detect and quantify c-Fos protein.
Experiments on day 2
c-Fos expression in sires on day 2
F1,68 = 25.83
P = 0.0000
F2,68 = 19.42
P = 0.0000
Behavior and condition
F2,68 = 25.25
P = 0.0000
F1,65 = 10.27
P = 0.0021
F2,65 = 23.70
P = 0.0000
Behavior and condition
F2,65 = 18.02
P = 0.0000
F1,59 = 1.38
P = 0.2455
F2,59 = 19.47
P = 0.0000
Behavior and condition
F2,59 = 3.49
P = 0.0368
F1,69 = 0.00
P = 0.9544
F2,69 = 5.99
P = 0.0040
Behavior and condition
F2,69 = 4.10
P = 0.0208
Alternative comparisons are shown in Figure 5D and E. The number of c-Fos positive cells was the highest in the S6-3 conditions among three isolation conditions in retrievers (Figure 5E), compared to non-retrievers (Figure 5D). Significant differences were found between S6-1 vs S6-2, S6-1 vs S6-3, or S6-2 vs S6-3 at *P < 0.05 and ***P < 0.001, respectively, two-way Student t-test. These data show that the mPOA-VTA-NAcc-VP circuit in retriever sires is likely to be sensitized by the dams' communicative interaction.
The statistical analysis results on time course of c-Fos expression obtained by two-way ANOVA (corresponding to Figure 6 )
F1,139 = 111.70
P = 0.0000
F3,139 = 56.02
P = 0.0000
Behavior and time
F3,139 = 22.57
P = 0.0000
F1,113 = 0.85
P = 0.3598
F3,113 = 14.21
P = 0.0000
Behavior and time
F3,113 = 0.37
P = 0.7768
F1,104 = 0.30
P = 0.5865
F3,104 = 32.07
P = 0.0000
Behavior and time
F3,104 = 0.87
P = 0.4598
F1,130 = 6.40
P = 0.0126
F3,130 = 22.67
P = 0.0000
Behavior and time
F3,130 = 1.44
P = 0.2345
c-Fos expression after retrieving tests by USVs and pheromones in the mPOA
These results demonstrate that contrasting the retriever and non-retriever sires allowed us to determine the effect of a single retrieval session on mPOA activity, a region known to regulate pup-induced parental behavior ,,. Our result is consistent with data showing the critical role of the mPOA in pup-induced maternal and paternal behavior in mice and other mammals  .
c-Fos-positive cells in the mPOA in sires with or without stimulation of USV or pheromones under no influence of pups
c-Fos positive nuclei/0.1 mm2
7.0 ± 0.32
17.3 ± 1.95***
6.2 ± 0.7
13.9 ± 1.22**
Expression of c-Fos induced by exposure to pups has been reported in other brain regions of interest that are associated with vole paternal behavior , including the medial nucleus of the amygdala, the lateral septum, the medial bed nucleus of the hypothalamus and the accessory olfactory bulb. In rats, experimental evidence has revealed that some of these brain regions are involved in promoting the expression of maternal behavior ,,. Whether c-Fos expression is induced by communicative interaction in the above mentioned brain regions should be determined.
Our third conclusion from the second day of experiments is that the number of cells expressing c-Fos in the S6-3 condition is higher than those in the S6-2 condition, with average increases by communicative interaction over no interaction ((cell density in S6-3)/(cell density in S6-2) ×100) of: 154 ±45% (P = 0.000), 860 ±23% (P = 0.000), 253 ±41% (P = 0.000) and 555 ±13% (P = 0.000) in the mPOA, VTA, NAcc and VP regions, respectively (Figure 5B and C). This increase appears to be a net difference caused by the presence of the mate dam in the cage together with the sire, although transferring to new cages condition (environment change) was equal. However, our previous report showed that retrieval behavior is usually observed in the S6-1 condition . If we assume that the induction of c-Fos in the mPOA is responsible for paternal behavior, c-Fos expression should be higher in the mPOA. The reason for this discrepancy is unclear at this moment. This point should be examined in the near future.
Approximately 30 to 60 min is required to achieve peak mRNA expression of c-fos and 90 120 min is required to achieve peak c-Fos protein expression after exposure to a stimulus  . We examined c-Fos protein expression approximately 30 min after the start of stimulation in most of the current experiments, because quantification was applicable with relatively high accuracy by the current (Metamorph) software. We selected this short duration to reflect the direct interaction between pairmates by controlling environment-related effects on brain neuronal activity. To support using the 30 min duration, we examined c-Fos protein induction after 30, 60, and 120 min by leaving the sires in the retrieval test condition (S4-1 and S4-2). Although the number of c-Fos positive cells was high at 60 min, a duration of 30 min was sufficient to induce expression in two regions, and a duration of 120 min resulted in a slight decline (Figure 6, brownish color bars). Furthermore, the mean percentage increase of the c-Fos positive cell number in the retrievers over that in the non-retrievers was relatively unchanged at each time point: Two-way ANOVA for retrieval behaviors and time course (Table 2). Thus, these results may allow for a comparison of c-Fos levels at the 30-min time duration.
To compare c-Fos protein levels before and after exposure to stimuli using the protocol by Numan and Numan , where the subject mother or father rats were separated from pups for two days to achieve complete degradation of c-Fos protein, requires >6 hours. In our experiments, we were unable to use this protocol to reduce c-Fos because any changes in the family situation altered the behavior of the sires. Although we were unable to determine the basal c-Fos level of the sires from which induction of c-Fos may occur, our method was able to measure c-Fos levels at the S1 condition before the start of the S2 condition. The level at S1 was significantly lower than S4 condition.
It is likely that the mPOA-VTA-NAcc-VP neural circuit in sires induces paternal behavior, although we could not determine the circuit sequence. Further studies are required to detail the role of this neural circuit in the regulation of paternal behavior in mice under this paradigm.
Male and female Slc:ICR mice were obtained from Japan SLC, Inc. (Hamamatsu, Japan) via a local distributor (Sankyo Laboratory Service Corporation, Toyama, Japan). The offspring of these mice were born in our laboratory colony and housed until pairing. The animals were paired and kept in our laboratory under standard conditions (22°C; 12-h light/dark cycle, lights on at 8:45 a.m.) in standard mouse cages (300 mm 160 mm 110 mm), with sawdust bedding, with as food and water provided ad libitum, as previously described ,. All of the animal experiments were approved and performed in accordance with the Fundamental Guidelines of the Committee on Animal Experimentation of Kanazawa University.
Virgin males and females were paired at 45-55 d and continuously housed together in a standard mouse maternity cage until after the delivery of pups on postnatal day 3-5. The average litter size of the dams was 13.5 ± 0.67 (n = 30) . Each of the family units, composed of the new sire and dam and their first litter, were experimentally naïve and used once (Figure 1, S1).
The parents were isolated from the pups to treat them in new cages (environments) for 10 min  (S2), in which we detected communicative interaction from the dam to the sire via 38-kHz ultrasonic vocalization and unidentified pheromones ,. Five pups were randomly selected from the litter and placed individually at a site remote from the nest in the original family cage. After 10 min in the new cage, each sire was returned to its home cage that housed his five biological pups to assess parental retrieval behavior (S3). The percentage of sires exhibiting retrieval behavior was quantified during the 10 min following reunion with the pups (S4-1 or S4-2). The sires that carried all five of their pups to the original nesting place or within two thirds of the distance between the nest and the new location were defined as retrieval-positive or retrievers (S4-2) ,. The sires that did not fulfill the above behavioral definition were designated as retrieval-negative or non-retrievers (S4-1). The behavioral tests were conducted at 10:00 15:00 h in a randomly mixed sequence of experimental groups.
In the second set of experiments, we performed retrieval experiments, as above (S1 - S4), and selected the families with the sires exhibiting retrieval (S4-2). The following day, the parents (S5) were isolated from the pups under one of the following conditions: 1) the sire was left alone in the family cage, in which family cues were present, without direct interaction with his mate (S6-1); 2) the sire was placed alone without communicative interaction in a new environment (S6-2); or 3) the sire and dam were co-housed under communicative interaction in a new environment (S6-3).
After each experiment, the sires were anesthetized, sacrificed and prepared for c-Fos immunohistochemistry. The mice were intracardially perfused with cold PBS followed by a cold 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) solution. The brains were removed and post-fixed in a 4% PFA solution overnight at 4°C. Brain regions were cut into two to four larger blocks. The blocks were sliced on a microtome into 20-μm-thick sections. The sections were pre-incubated in blocking solution (3% bovine serum albumin and 0.3% Triton X-100 in PBS) for 1 h, then incubated with an anti-c-Fos antibody (sc-52, 1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in blocking solution for 12 h at 4°C. After three washes with washing buffer, the sections were incubated with goat anti-rabbit IgG antibody coupled with Alexa Fluor 488 (Invitrogen, Carlsbad, CA, USA) in blocking solution for 1 h at room temperature. The images were obtained using an Olympus IX71 inverted microscope equipped with a cooled CCD camera (Cool SNAP HQ2; Roper Scientific, Tucson, AZ, USA). The number of c-Fos immuno-positive nuclei in each brain section was recorded and analyzed using Metamorph software (Molecular Devices, Downingtown, PA, USA), as described previously ,. Only the c-Fos-positive nuclei within a specific size range were counted and a constant threshold level of staining was used to distinguish c-Fos-positive cells as follows: a fluorescence diameter < 13.5 μm and intensity above 445.6 (arbitrary units) were counted. The average fluorescence intensity was within the 5-fold range (Figures 2, 3 and 4).
The distance from the bregma in the rostrocaudal plane is 0.14 mm for the mPOA, -3.08 mm for the VTA, 1.18 mm for the NAcc and 0.74 mm for the VP, according to Keith et al. . The locations of the four brain regions and two primary sensory brain areas  are specified in the lower magnification diagram in Figure 2.
Two-tailed Student's t-test was used for the single comparisons between the two groups. The remaining data were analyzed using one-way or two-way analyses of variance (ANOVA). Post-hoc comparisons were performed only when the main effect was statistically significant. The P-values for multiple comparisons were adjusted using the Bonferroni correction.
JZ, ML, SA, TT and CH conducted the experiments and analyzed all of the data. HH designed the study and wrote the manuscript. All of the authors read and approved the final manuscript.
This work was supported by the Strategic Research Program for Brain Sciences from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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