Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells
Molecular Brain volume 16, Article number: 41 (2023)
The primary cilium, an antenna-like structure on the cell surface, acts as a mechanical and chemical sensory organelle. Primary cilia play critical roles in sensing the extracellular environment to coordinate various developmental and homeostatic signaling pathways. Here, we showed that the depletion of heat shock protein family A member 9 (HSPA9)/mortalin stimulates primary ciliogenesis in SH-SY5Y cells. The downregulation of HSPA9 enhances mitochondrial stress by increasing mitochondrial fragmentation and mitochondrial reactive oxygen species (mtROS) generation. Notably, the inhibition of either mtROS production or mitochondrial fission significantly suppressed the increase in primary ciliogenesis in HSPA9-depleted cells. In addition, enhanced primary ciliogenesis contributed to cell survival by activating AKT in SH-SY5Y cells. The abrogation of ciliogenesis through the depletion of IFT88 potentiated neurotoxicity in HSPA9-knockdown cells. Furthermore, both caspase-3 activation and cell death were increased by MK-2206, an AKT inhibitor, in HSPA9-depleted cells. Taken together, our results suggest that enhanced primary ciliogenesis plays an important role in preventing neurotoxicity caused by the loss of HSPA9 in SH-SY5Y cells.
Cilia, which can be categorized into motile and non-motile primary cilia, are dynamically regulated but highly conserved organelles. In most cases, motile cilia have a 9 + 2 microtubule structural arrangement, whereas non-motile primary cilia have a 9 + 0 microtubule structural arrangement [1, 2]. Cilia are cellular organelles primarily composed of a membrane, soluble compartment, axoneme, basal body, and ciliary tip [1, 2]. Primary cilia are mechanical and chemical sensory organelles that play critical roles in sensing the extracellular environment to coordinate developmental and homeostatic signaling pathways [1, 2]. Primary cilia are implicated in the regulation of essential signal transduction mechanisms that control a wide variety of cellular events, including sonic hedgehog (Shh) , Wnt [4, 5], TGF-β , and platelet-derived growth factor (PDGF)-mediated cell signaling . PDGF receptor alpha (PDGFRα) signaling in the primary cilium regulates the AKT (also known as protein kinase B/PKB) signaling pathway . Furthermore, the dysregulation of primary cilia functions caused by the loss of ciliary proteins increases cell death, whereas enhanced primary ciliogenesis promotes cell survival under various stress conditions [9, 10]. Thus, disrupting the regulatory functions of cilia seems to underlie a diverse spectrum of human disorders, the primary ciliopathies. Ciliopathies include numerous seemingly unrelated developmental syndromes and manifest in the retina, kidney, liver, pancreas, skeletal system, and the brain [2, 11].
Structurally, the primary cilium is composed of functional domains, including the basal bodies, transition fibers, transition zone, intraflagellar transport (IFT) machinery, axoneme, and the ciliary membrane. IFT particles are large complexes comprising subcomplexes A and B, which mediate the bidirectional movement of protein cargo along the axonemal microtubules. Mutations in the IFT proteins of the cilia result in a group of inherited human diseases referred to as ciliopathies. IFT88, a core anterograde protein, is critical for cilia assembly and maintenance . In addition to IFT proteins, ADP-ribosylation factor-like protein 13B (ARL13B) and Smoothened (Smo) are also mainly localized to the primary cilia. Thus, these proteins are commonly used as monitoring markers of primary cilia .
Recent studies have revealed a close relationship between primary cilia and mitochondrial dysfunction. The mTOR pathway associates cellular energy with mitochondrial biogenesis and ciliary length . It has been found that mitochondrial dysfunction compromises ciliary homeostasis in astrocytes. Moreover, the loss of primary cilia promotes mitochondria-dependent apoptosis in thyroid cancer . Our group recently showed that primary cilia mediate mitochondrial stress responses and autophagy to promote dopamine neuron survival in a Parkinson’s disease model . Reactive oxygen species (ROS) also regulate primary cilium length during kidney injury caused by ischemia/reperfusion insult . Hence, growing evidence indicates an interplay between primary cilia and mitochondrial function. Nonetheless, the mechanism underlying the interplay between primary cilia and mitochondria is still largely unknown.
In this study, we screened a mitochondrial protein library and found that the loss of heat shock protein family A (Hsp70) member 9 (HSPA9), also known as mortalin, induces primary ciliogenesis. Depletion of HSPA9 induces mitochondrial stress by increasing mitochondrial fragmentation and mitochondrial ROS (mtROS) generation. In addition, we found that enhanced primary ciliogenesis due to mitochondrial stress in HSPA9-deficient SH-SY5Y cells prevents neuronal cell death by activating AKT.
Materials and methods
Reagents and gene knockdown
Doxycycline (D9891) and N-acetylcysteine (NAC, A9165) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(2,4-dichloro-5-methoxyphenyl)-2,3-dihydro-2-thioxo-4(1 H)-quinazolinone (Mdivi-1, BML-CM127) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). MK-2206 (S1078) was purchased from Selleck Chemicals (Houston, TX, USA). For gene expression knockdown, cells were transfected with validated small inhibitory RNAs (siRNAs) targeting human HSPA9 (5′-AAACGCAAGUGGAAAUUAA-3′), Drp1 (5′-GAGGUUAUUGAACGACUCA-3′), or IFT88 (5′-CCGAAGCACUUAACACUUA-3′) using Lipofectamine 2000. The siRNAs were synthesized by Genolution (Seoul, Korea). At 48 h post-transfection, the cells were treated with the indicated reagents.
Cell lines and primary cell culture
SH-SY5Y neuroblastoma cells were obtained from ATCC (Manassas, VA, USA). Human telomerase-immortalized retinal pigmented epithelial (RPE) cells were kindly provided by Dr. Jun Kim (KAIST, South Korea). TT cells stably expressing lentiviral doxycycline-inducible small hairpin RNA(shRNA) targeting HSPA9 (TT/shHSPA9) were kindly provided by J.I. Park (University of Wiscosin-Milwaukee, USA). To generate stable cell lines, SH-SY5Y cells were transfected with pMito-HyPer (SY5Y/Mito-HyPer) using Lipofectamine 2000, according to the manufacturer’s protocol (#11668019, Thermo Fisher Scientific, Waltham, MA). Positive transfectants were selected via growth in a medium containing 1 mg/mL G418 (#10131027, Thermo Fisher Scientific) for 7 days. After single-cell dropping, stable clones were selected under a fluorescence microscope (IX71, Olympus, Tokyo, Japan).
Cilia staining and counting
For the staining of primary cilia, the cells were washed with cold phosphate-buffered saline (PBS) and fixed with 4% (w/v) paraformaldehyde, which was dissolved in PBS containing 0.1% (v/v) Triton X-100. Subsequently, cells were blocked with PBS containing 1% bovine serum albumin (BSA) and incubated overnight at 4 °C with primary antibodies against acetylated α-tubulin (1:1000; T7451, Sigma-Aldrich), γ-tubulin (1:1000; T5326, Sigma-Aldrich), and ARL13B (1:1000; 17711-1-AP, Proteintech) in 1% BSA. After washing, the cells were incubated with Alexa Fluor 488- or 555-conjugated secondary antibodies at room temperature (RT) for 1 h. Before mounting, the cells were treated with Hoechst 33,342 dye (1:10000, H3570, Thermo Fisher Scientific) for nuclear staining. Cilia were observed using a fluorescence microscope. Cilia were counted in approximately 200 cells for each experimental condition (n = 3). The ciliated cell percentage was calculated as follows: (total number of cilia/total number of nuclei in each image) × 100. Cilia lengths were measured using the free-hand line selection tool of Cell Sense Standards software (Olympus Europa Holding GmbH, Hamburg, Germany), and the average cilium length was calculated.
Western blot analysis
Cell lysates were prepared in 2× Laemmli sample buffer [62.5 mM Tris-HCl, pH 6.8, 25% (v/v) glycerol, 2% (w/v) SDS, 5% (v/v) β-mercaptoethanol, and 0.01% (w/v) bromophenol blue] (#161–0737, Bio-Rad, Hercules, CA, USA). After separation via 10–12% SDS-PAGE, the proteins were transferred onto a PVDF membrane (#162–0177, Bio-Rad). The membranes were then incubated with the following primary antibodies: Drp1 (#611738, BD), IFT88 (13967-1-AP, Proteintech, Chicago, IL, USA), HSPA9 (Sc-13967, Santa Cruz Technology, CA, USA), Gli2 (18989-1-AP, Proteintech) p-AKT (#9271S, Cell Signaling Technology), cleaved caspase-3 (#9661S, Cell Signaling Technology), and actin (MAB1501, Millipore, Temecula, CA, USA). For protein detection, the membranes were incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Pierce, Rockford, IL, USA). Chemiluminescent signals were developed using Clarity Western ECL substrate (W3680-010, Bio-Rad).
Determination of cellular ROS and mitochondrial ROS levels
Intracellular ROS levels were assayed using the fluorescent dye 2,7-dichlorofluorescein diacetate (DCFH-DA) (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. DCFH-DA is converted to the highly fluorescent compound 2,7-dichlorofluorescein (DCF) in the presence of oxidants. Briefly, SH-SY5Y cells were plated in 96-well plates, transfected with siRNA, and incubated with DCFH-DA (20 µM) in the presence or absence of N-acetyl-L-cysteine (NAC). The relative ROS ratios were measured using a fluorescence microplate reader (Victor X3, PerkinElmer, Waltham, MA, USA). The levels of mitochondria-specific ROS were assessed using the HyPer protein system. The pHyPer-dMito vector encoding mitochondria-targeted HyPer (Mito-HyPer) was obtained from Eyrogen (San Diego, CA, USA). SH-SY5Y cells stably expressing Mito-HyPer were transfected with scrambled or HSPA9-specific siRNAs for 72 h in the presence or absence of NAC or Mito-Q. The fluorescence intensities were monitored using a fluorescence plate reader (excitation 500 nm/emission 516 nm) (Victor X3) or fluorescence microscopy.
Measurement of mitochondrial length
For the staining of mitochondria, cells were fixed with 4% PFA and then treated with a MitoTracker probe (100 nM, M7512, Thermo Fisher Scientific) for 30 min. Mitochondrial images were obtained using a fluorescence microscope (IX71; Olympus, Japan). The mitochondrial length was measured using the free-hand line selection tool of Cell Sense Standards software (Olympus Europa Holding GmbH). The mean length of the mitochondria was determined by selecting 20–30 linearized and unconnected filament-like mitochondria per cell using a tool provided by the Cell Sense Standards software (n = 3 independent experiments). Images of individual cells were analyzed and digitized using GraphPad Prism 8 (GraphPad Software, San Diego, CA, USA).
Cell viability analysis
For the cell proliferation assay, SH-SY5Y cells seeded in 96-well plates were transfected with HSPA9 siRNA. After transfection, the cell proliferation rate was measured daily for using a Cell Counting Kit-8 (CCK8) solution reagent (10 µM) (Dojindo Laboratories, Kumamoto, Japan) for 2 h, following the manufacturer’s instructions. Absorbance was measured using a spectrophotometer (Victor-X3, PerkinElmer).
Statistical analyses of the results were performed using one-way analysis of variance (ANOVA) followed by a post-hoc least significant difference (LSD) test or an unpaired Student’s t-test using Origin software (San Clemente, CA, USA) or GraphPad Prism 8 (GraphPad Software, San Diego, CA, USA). Data were obtained from at least three independent experiments and are presented as the mean ± standard error of the mean (SEM). Statistical significance was defined as p < 0.05.
Depletion of HSPA9 induces primary ciliogenesis
Recently, it has been shown that primary cilia are involved in mitochondrial stress and apoptosis [9, 10]. However, the interplay between the primary cilia and mitochondria remains unknown. To identify novel modulators of ciliogenesis in mitochondria, we screened a small siRNA library consisting of mitochondrial proteins using htRPE cells that stably expressed GFP-fused Smo (htRPE/Smo-GFP), which is widely used as a cilium marker. Based on this screening, we selected siRNAs for HSPA9/mortalin as a potent novel inducer of ciliogenesis. HSPA9 is primarily localized to the mitochondria and is involved in multiple mitochondrial processes, including energy metabolism, free-radical generation, and maintenance of mitochondrial protein integrity . However, the role of HSPA9 in cilia has not been elucidated.
To confirm the screening results, htRPE/Smo-GFP cells were transiently transfected with scrambled or specific siRNA against HSPA9. Consistent with the screening results, HSPA9 depletion resulted in enhanced primary cilia in htRPE cells (Fig. 1A, B). Tubulin undergoes various post-translational modifications, including acetylation and glutamylation, and these modifications are highly enriched in microtubules of the axoneme in primary cilium . Thus, we further examined the levels of acetylated α-tubulin by depletion of HSPA9 in RPE cells. As shown in Fig. 1C, not only ARL13B but also acetylated α-tubulin were increased in HSPA9 knockdown cells (Fig. 1C). Then the promotion of primary ciliogenesis was further confirmed using a doxycycline-induced shRNA knockdown system . Consistent with previous report, treatment with doxycycline efficiently reduced the expression of HSPA9 in the TT/shHSPA9 cells (Fig. 1D). Subsequently, the primary ciliogenesis was enhanced by treatment with doxycycline in the TT/shHSPA9 cells (Fig. 1E, F). Previously, we showed that decreased HSPA9 expression is associated with neurodegenerative diseases, such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) [18, 19]. Thus, we investigated whether HSPA9 affected primary ciliogenesis in SH-SY5Y neuroblastoma cells. Consistent with the effects observed in RPE cells, the primary cilium length was robustly increased after HSPA9 knockdown in SH-SY5Y cells (Fig. 2A, B).
IFT88/polaris is a component of the IFT-B protein complex that mediates antegrade IFT, where a lack or hypomorphic mutation in IFT88 disrupts the cilia assembly . Thus, we further addressed the effect of HSPA9 on primary ciliogenesis by depleting IFT88 expression. According to a previous study, knockdown of the ciliary core protein IFP88 blocked primary ciliogenesis in HSPA9-deficient SH-SY5Y cells (Fig. 2A, B). Interestingly, we found that the depletion of HSPA9 induced AKT phosphorylation, which was also blocked by IFT88 knockdown (Fig. 2C), Recently, it was reported that phosphorylated AKT localizes at the ciliary base centrosome to recruit ciliary protein . We next examined the localization of phospho-AKT in HSPA9-knockdown cells and found that phospho-AKT is co-localized with γ-Tubulin (Fig. 2D), suggesting that AKT is activated by promoting primary ciliogenesis in HSPA9-deficient cells. Taken together, these results suggest that the loss of HSPA9 influences induction of primary ciliogenesis.
Increased mitochondrial ROS levels influence primary ciliogenesis via the depletion of HSPA9 in SH-SY5Y cells
Next, we elucidated the molecular mechanisms underlying HSPA9 depletion-induced primary ciliogenesis. Previously, we showed that HSPA9 deficiency increased the generation of ROS by increasing mitochondrial fragmentation, which promoted primary ciliogenesis . Therefore, we investigated the effect of HSPA9 knockdown on ROS generation in SH-SY5Y cells. Cellular ROS levels were considerably increased upon HSPA9 knockdown in SH-SY5Y cells; however, excessive ROS was removed after treatment with N-acetylcysteine (NAC), an ROS scavenger (Fig. 3A). Since dysfunctional and fragmented mitochondria generate higher levels of ROS, we further analyzed mtROS using the expression of a mitochondrial hydrogen peroxide sensor (mt-HyPer). HSPA9 knockdown potently increased mtROS levels in SH-SY5Y cells. In addition, treatment with the mitochondria-targeted antioxidant drug mitoquinone (Mito-Q) blocked mtROS overproduction caused by the loss of HSPA9 in SH-SY5Y cells (Fig. 3B). Notably, we found that HSPA9 knockdown-induced primary ciliogenesis was strikingly suppressed upon treatment with the ROS scavengers NAC and Mito-Q (Fig. 3C, D), suggesting that mtROS critically mediates mitochondrial ROS-induced ciliogenesis by depleting HSPA9 in SH-SY5Y cells.
Significant mitochondrial fragmentation is induced by excessive mtROS production, and we previously reported that the knockdown of HSPA9 increases mitochondrial fission in a neurodegenerative disease model . Thus, we investigated a possible link between mitochondrial fission/fusion and ciliogenesis following HSPA9 depletion. Mitochondrial dynamics are promoted by the GTPase family protein dynamin-1-like protein (Drp1) . Consistent with previous reports, mitochondrial staining showed that HSPA9 knockdown promoted mitochondrial fragmentation in SH-SY5Y cells (Fig. 4A). In addition, either genetic or chemical inhibition of Drp1 by RNA interference or Mdivi-1 treatment, respectively, efficiently blocked mitochondrial fragmentation in HSPA9-depleted cells (Fig. 4A, B). We further explored the effect of inhibiting mitochondrial fragmentation on primary ciliogenesis. Notably, both genetic and chemical inhibition of Drp1 significantly suppressed ciliated cells and cilium length in HSPA9-depleted cells (Fig. 4C, D). Taken together, these results suggest that mitochondrial ROS and fission regulate primary ciliogenesis induced by HSPA9 loss in SH-SY5Y cells.
Enhanced primary ciliogenesis prevents neuronal cell death by activating AKT in HSPA9-depleted SH-SY5Y cells
Excessive mitochondrial fission has been implicated in a wide range of human disorders by increasing the susceptibility of cells to mitochondrial stress and apoptosis [23, 24]. In contrast, primary cilia can promote cell survival under various stress conditions, including oxidative stress . In addition, the inhibition of HSPA9 has been shown to induce mitochondrial stress and cancer cell death [15, 25, 26]. Thus, we further addressed the effects of blocking enhanced primary ciliogenesis on HSPA9 deficiency-induced cell death. As shown in Fig. 5A, B, the downregulation of HSPA9 alone slightly induced the cleavage of caspase-3 and cell death in SH-SY5Y cells. However, the loss of primary cilia by co-knockdown with IFT88 further increased cell death and caspase-3 activation (Fig. 5A, B). Previously, we found that the downregulation of HSPA9 increased the phosphorylation of AKT/PKB, which promoted survival of SH-SY5Y cells. Thus, we further evaluated the role of AKT activation in cell death through the depletion of HSPA9. When IFT88 was depleted, both caspase-3 activation and cell death were enhanced after treatment with the selective AKT inhibitor MK-2206, compared to depletion of HSPA9 alone (Fig. 5C, D), indicating that enhanced primary ciliogenesis after HSPA9 knockdown promotes cell survival by activating AKT in SH-SY5Y cells.
In this study, we found that the loss of HSPA9/mortalin induced primary ciliogenesis in SH-SY5Y cells. HSPA9 is a multipotent stress response chaperone protein induced by metabolic stress, glucose deprivation, calcium ionophores, thyroid hormone treatment, hyperthyroidism, ionizing radiation, and several cytotoxins . Thus, it has been implicated in various cellular functions, including stress response, cell proliferation control, and apoptosis inhibition. In particular, HSPA9 regulates the functions of the tumor suppressor protein p53 and plays an important role in the stress response and maintenance of the mitochondria [17, 27]. However, the role of HSPA9 in primary ciliogenesis has not been elucidated.
We recently reported an interplay between two seemingly irrelevant organelles, primary cilia and mitochondria . Mitochondrial stress-induced ciliogenesis is mediated by mtROS generation, which subsequently activates AMP-activated protein kinase (AMPK) and autophagy. However, the abrogation of ciliogenesis compromises mitochondrial stress-induced autophagy, leading to enhanced cell death in vitro and in vivo . We further reported that the loss of HSPA9 in AD models potentiates mitochondrial dysfunction by increasing mtROS generation and mitochondrial fission . Based on these previous reports, we confirmed that HSPA9 depletion increased mtROS and mitochondrial fragmentation (Figs. 3 and 4). In addition, our data suggest that the depletion of HSPA9 induced primary ciliogenesis in SH-SY5Y cells (Figs. 1 and 2). Notably, we also found that disruption of cilium by IFT88 knockdown upregulated the expression of HSPA9 (Figs. 2C and 5 A). Although the effect of impaired primary cilium on HSPA9 function has not been elucidated, recent reports suggest that loss of IFT88 potentiates mitochondrial dysfunctions including decrease of oxidative phosphorylation reaction and increase of mitochondrial fragmentation in various cells [28, 29]. Therefore, the crosstalk between the expressional regulation of HSPA9 and IFT88 will be further elucidated. Moreover, our results indicated that HSPA9 depletion enhanced primary ciliogenesis to suppress neurotoxicity during mitochondrial stress, decreasing mtROS levels and mitochondrial fission (Figs. 3 and 4).
The enhanced ciliated cells and cilium length were completely abolished by inhibiting either mtROS or mitochondrial fission in SH-SY5Y cells. Our findings indicate that ciliogenesis was enhanced in response to mitochondrial stress in SH-SY5Y cells. Furthermore, the cytoprotective effect of primary cilia is mediated, at least in part, through the activation of AKT in SH-SY5Y cells (Fig. 5). Our findings suggest that ciliogenesis may be an important adaptive or defensive mechanism against mitochondrial stress insults in neuronal cells. AKT is one of the most well-characterized kinases because of its critical role in regulating numerous cellular functions, including metabolism, development, proliferation, transcription, protein synthesis, and cell survival . Although the precise mechanism of the ciliary signals that maintain the connectivity and viability of neurons remains unknown, primary cilia and ciliary signals play various essential roles during the development of the brain and central nervous system. Notably, it has been reported that primary cilia prevent environmental stress-induced dendritic degeneration by activating AKT signaling, which inhibits caspase-3 activation in the neonatal mouse forebrain . Bowie and Goetz showed that primary cilia with TTBK2 are essential for the survival and connectivity of cerebellar Purkinje neurons . Accordingly, we also found that enhanced primary cilia prevented neurotoxicity by activating AKT in HSPA9-depleted cells (Fig. 5). The loss of primary cilia additionally induced caspase-3 activation and cytotoxicity in HSPA9-depleted SH-SY5Y cells (Fig. 5A, B). In addition, both caspase-3 and cell death were significantly potentiated by AKT inhibition (Fig. 5C, D).
Recently, our group also reported that the loss of HSPA9 induces peroxisomal autophagy (pexophagy) by increasing peroxisomal stress in neuronal cells . Excessive HSPA9 depletion generates ROS to promote pexophagy and mitochondrial autophagy (mitophagy) [19, 33]. The ectopic expression of several loss-of-function mutants of HSPA9 found in patients with PD and HSPA9-deficient cells failed to rescue mitochondrial dysfunction and phagocytic activity [19, 33]. Mitochondrial and peroxisomal dysfunction play major roles in the pathogenesis of PD [19, 34]. PD-associated mitochondrial dysfunction can result from diverse causes such as impaired mitochondrial biogenesis, increased ROS and mitochondrial fission, and defective mitophagy . As mitochondrial stress-induced ciliogenesis is mediated by mtROS, which activates autophagy, further exploration of the role of HSPA9 in the regulation of primary ciliogenesis will be helpful in understanding neurodegenerative diseases, including PD. Taken together, our present findings extend the knowledge of HSPA9 in the repertoire of cilia-related biological functions and diseases.
All of the data generated and analyzed in this study are included in this published article.
heat shock protein family A member 9
mitochondrial reactive oxygen species
platelet-derived growth factor
intraflagellar transport (IFT)
ADP-ribosylation factor-like protein 13B
retinal pigmented epithelial
small hairpin RNA
GTPase family protein dynamin-1-like protein
AMP-activated protein kinase
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We thank Dr. Joon Kim (KAIST, South Korea) and Dr. J.I. Park (University of Wiscosin-Milwaukee, USA) for providing RPE and TT cells with a doxycycline-induced deletion of HSPA9 (TT/shHSPA9), respectively.
This research was supported by the National Research Foundation of Korea funded by the Ministry of Science and ICT [2020R1A2C2003523, and 2020R1A4A1018280] and Ministry of Education [2020R1I1A1A01074053].
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Bae, JE., Jang, S., Kim, J.B. et al. Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells. Mol Brain 16, 41 (2023). https://doi.org/10.1186/s13041-023-01029-7