Acute 5-HT7 receptor activation increases NMDA-evoked currents and differentially alters NMDA receptor subunit phosphorylation and trafficking in hippocampal neurons
Molecular Brain volume 6, Article number: 24 (2013)
N-methyl-D-aspartate (NMDA) receptors are regulated by several G protein-coupled receptors (GPCRs) as well as receptor tyrosine kinases. Serotonin (5-HT) type 7 receptors are expressed throughout the brain including the thalamus and hippocampus. Long-term (2–24 h) activation of 5-HT7 receptors promotes the expression of neuroprotective growth factor receptors, including the platelet-derived growth factor (PDGF) β receptors which can protect neurons against NMDA-induced neurotoxicity.
In contrast to long-term activation of 5-HT7 receptors, acute (5 min) treatment of isolated hippocampal neurons with the 5-HT7 receptor agonist 5-carboxamidotryptamine (5-CT) enhances NMDA-evoked peak currents and this increase in peak currents is blocked by the 5-HT7 receptor antagonist, SB 269970. In hippocampal slices, acute 5-HT7 receptor activation increases NR1 NMDA receptor subunit phosphorylation and differentially alters the phosphorylation state of the NR2B and NR2A subunits. NMDA receptor subunit cell surface expression is also differentially altered by 5-HT7 receptor agonists: NR2B cell surface expression is decreased whereas NR1 and NR2A surface expression are not significantly altered.
In contrast to the negative regulatory effects of long-term activation of 5-HT7 receptors on NMDA receptor signaling, acute activation of 5-HT7 receptors promotes NMDA receptor activity. These findings highlight the potential for temporally differential regulation of NMDA receptors by the 5-HT7 receptor.
N-methyl-D-aspartate (NMDA) receptors are tetrameric channels composed of two NR1 and two NR2 or NR3 subunits . In the hippocampus, most NR2 subunits are either NR2A or NR2B  and there is evidence that heterotrimeric NMDA receptors containing both NR2A and NR2B are also present . Several studies have examined the ability of serotonin (5-HT) receptors to modulate NMDA receptor activity. For example, in isolated cortical neurons, activation of 5-HT1A receptors inhibits NMDA receptor currents  and 5-HT3 receptor activation reduces NMDA receptor currents in cortical slices . In contrast, in Xenopus oocytes, 5-HT2 receptor activation increases NMDA receptor currents  and in prefrontal cortical slices, 5-HT2A/2C agonists enhance NMDA-evoked responses .
Although first identified in the suprachiasmatic nucleus, 5-HT7 receptors are expressed throughout the CNS, including the hippocampus . The effect of 5-HT7 receptor ligands on NMDA-evoked currents remains unknown however recent studies provide clear evidence for the regulation of glutamatergic signaling by 5-HT7 receptors. 5-HT7 receptors inhibit NMDA-induced neurotransmitter release in the dorsal raphe nucleus (DRN) and the physiological role of 5-HT7 receptors in circadian rhythms is associated with an inhibition of glutamate-dependent events [9, 10]. In the suprachiasmatic nucleus, glutamate excitatory post-synaptic potentials (EPSPs) and glutamate-induced intracellular calcium levels are both inhibited by 5-HT7 receptor activation [11, 12]. Taken together, these studies suggest that 5-HT7 receptor activation decreases NMDA and/or glutamate receptor signaling. In contrast, compared to wild-type, 5-HT7 receptor knock-out mice display a reduced induction of long-term potentiation (LTP), magnitude of LTP, and hippocampus-associated learning . Therefore, although there is evidence that 5-HT7 receptors negatively regulate NMDA/glutamate signaling, deletion of 5-HT7 receptors decreased the magnitude of NMDA receptor-dependent events such as LTP.
5-HT7 receptors are Gαs–coupled, although they may couple to additional Gα isoforms including Gα12[14, 15]. Recently we identified the 5-HT7 receptor as a regulator of platelet-derived growth factor (PDGF) β receptor expression and activity . Activation of PDGFβ receptors by PDGF-BB selectively inhibits NR2B-containing NMDA receptor currents and this may be involved in the mechanism of PDGFβ receptor-mediated neuroprotection . Intriguingly, 5-HT7 receptor-induced upregulation of the PDGFβ receptor was sufficient to protect neurons against NMDA-induced excitotoxicity . Thus, we proposed that long-term activation of 5-HT7 receptors initiates pathways that ultimately negatively regulate NMDA receptor signaling.
To clarify the direct effects of 5-HT7 receptor activation on NMDA receptor signaling we examined the effects of 5-HT7 receptor agonists and antagonists on NMDA-evoked currents, NMDA receptor subunit phosphorylation, and subunit cell surface expression in the hippocampus. In isolated hippocampal neurons, application of the 5-HT7 receptor agonist, 5-CT, resulted in a rapid and sustained increase in peak NMDA-evoked currents. 5-HT7 receptor agonist treatment also differentially altered NMDA receptor subunit phosphorylation and cell surface expression. These data, along with our previous work, suggest a model for differential NMDA receptor regulation by 5-HT7 receptors over the short- and long-term.
Application of the 5-HT7 receptor agonist, 5-CT, to isolated hippocampal neurons for 5 min increased NMDA-evoked peak currents (Figure 1). NMDA receptor currents were evoked once every 1 min using a 3 s exposure to NMDA (50 μM) and glycine (0.5 μM). 5-CT was applied in the bath continuously for 5 min after a 5-min stable baseline recording. This application increased NMDA-evoked currents to 142.5 ± 7.4% (n = 5) compared with baseline, whereas NMDA-evoked currents in control cells were stable over the recording period (95.9 ± 5.2%, n = 6) (p < 0.01, one way ANOVA (Tukey’s post hoc comparison), Figure 1). The 5-CT-induced increase in NMDA-evoked currents was observed within minutes after 5-CT application and the increase in peak currents was sustained even after 5-CT was washed out (min 10 through 30). In addition to 5-HT7, 5-CT also activates 5-HT1 and 5-HT5 receptors [19, 20]. However, the increase in NMDA-evoked currents by 5-CT was completely blocked by the 5-HT7 receptor-selective antagonist, SB 269970 (Figure 1).
To determine if 5-HT7 receptor activation altered NMDA receptor subunit phosphorylation we incubated hippocampal slices with 50 nM 5-CT in the absence or presence of SB 269970. 5-HT7 receptors are coupled to Gαs in several cell lines [14, 21–25] and the application of 5-CT to hippocampal slices robustly increased NR1 receptor phosphorylation at the PKA phosphorylation site, serine 897 (Table 1). 5-CT application also increased the phosphorylation of the NR1 subunit at PKC-site serine 896 (Table 1). The increase in the PKC-site serine 896 phosphorylation was blocked by the PKC inhibitor Go 6983 (Figure 2A) and the increase in the PKA-site serine 897 phosphorylation was blocked by the PKA inhibitor, H89 (Figure 2B). In Figure 1, a 5 min application of 5-CT resulted in a sustained elevation of NMDA-evoked currents for at least 20 min after washout. To determine if the observed changes in NR1 subunit phosphorylation were similarly sustained we treated hippocampal slices with 5-CT for 5 min, washed the slices, and waited an additional 20 min before homogenizing the tissue in lysis buffer. The phosphorylation state of both serines 896 and 897 remained significantly elevated above control for 20 min after the treatment with 5-CT (Figure 2C, D).
As mentioned above, 5-CT will also activate 5-HT1 and 5-HT5 receptors [19, 20]. To confirm that the observed changes in NMDA receptor subunit phosphorylation were indeed due to the activation of the 5-HT7 receptor we incubated acutely-dissected hippocampal slices with the 5-HT7 receptor-selective agonist, LP 12 , in the absence or presence of the 5-HT7 receptor antagonists, SB 269970 and SB 258719 . Similar to 5-CT, LP 12 increased NR1 receptor phosphorylation at the PKA phosphorylation site, serine 897 (Figure 3A, C) and this increased phosphorylation was sustained for at least 20 min (Figure 2D). Interestingly, we observed a larger increase in the phosphorylation of the adjacent, PKC-phosphorylated serine 896 (Figure 3B, C). However, the magnitude of the increase in serine 896 compared to serine 897 may be due to the lower basal phosphorylation state at serine 896. The increase in serine 896 phosphorylation was also observed for at least 20 minutes after LP 12 treatment (Figure 2C). The increased phosphorylation by LP 12 at both sites was blocked by the 5-HT7 receptor antagonists, SB 258719 (Figure 3) and SB 269970 (data not shown).
Application of LP 12 also differentially affected NR2 subunit phosphorylation. While LP 12 significantly reduced the phosphorylation state of the NR2A subunit (Figure 4A, B), LP 12 increased NR2B phosphorylation at tyrosine 1472 (Figure 4C, D). However the phosphorylation of NR2B at other tyrosine residues, 1252 and 1336, remained unchanged, with LP 12 inducing a 0.97 ± 0.12 (n = 12) and 0.93 ± 0.11 (n = 10) fold change, respectively, compared to control. The effects of LP 12 on the phosphorylation of NR2A and NR2B-Y1472 were both blocked by SB 258719 (Figure 4) and SB 269970 (data not shown). 5-CT treatment similarly enhanced NR2B-Y1472 phosphorylation and decreased NR2A phosphorylation (Table 1).
Several receptors and signaling pathways regulate NMDA receptor subunit cell surface expression. To investigate the effects of 5-HT7 receptor agonist treatment on the cell surface expression of the NMDA receptors subunits we treated hippocampal slices with LP 12 in the absence or presence of SB 258719 for 5 min. In hippocampal slices LP 12 treatment did not significantly change the cell surface expression of NR1 (0.92 ± 0.13 vs. control) and NR2A (1.05 ± 0.13 vs. control), but NR2B cell surface expression was reduced by approximately 25% and this effect was blocked by SB 258719 (Figure 5).
5-HT7 receptor knock-out mice display a reduced induction of LTP  and our finding that the acute 5-HT7 receptor activation increases NMDA-evoked currents may contribute to our understanding of how 5-HT7 receptors modulate synaptic plasticity. 5-HT7 receptor-mediated increases in NMDA-evoked currents may also contribute of our understanding of how 5-HT7 receptor activation potentiates bursting frequency in the CA3 hippocampal neurons  and increases CA1 extracellular population spike amplitude in CA1 hippocampal neurons . Conversely, there is evidence that 5-HT7 receptor agonists decrease glutamate-induced intracellular calcium release  and the amplitude of glutamate EPSPs . Based on the findings reported here and our previous report describing the ability of long-term 5-HT7 receptor agonist treatment to upregulate PDGFβ receptors [16, 18], we propose that the activation of 5-HT7 receptors may regulate NMDA receptor activity via two temporally distinct pathways (Figure 6): Acute (5 min) activation of 5-HT7 receptors increases NMDA-evoked currents whereas long-term (2–24 h) activation of 5-HT7 receptors upregulates PDGFβ receptors, a receptor tyrosine kinase that inhibits NMDA receptor activity [17, 18, 30].
5-HT7 receptors are involved in aspects of learning and memory associated with hippocampal function . For example, 5-HT7 receptor knock-out mice display impaired contextual fear-conditioning  and display a reduced ability to recognize new environments . These and other studies have promoted interest in 5-HT7 receptors as a potential drug target in Alzheimer’s disease and are further supported by a study demonstrating an increase in memory formation by a 5-HT7 receptor agonist, AS 19 . NMDA receptors are crucial components of learning and memory pathways in the hippocampus. Thus, the positive linkage between 5-HT7 receptors and NMDA receptor activity may explain how 5-HT7 receptors promote learning and memory as well as their involvement in LTP.
Since all three 5-HT7 receptor splice variants identified in rats are positively coupled with adenylate cyclase and display some level of constitutive activity , it is not surprising that the activation of 5-HT7 receptors in hippocampal slices increases the phosphorylation of the NR1 subunit at serine 897. On the NR2B subunit, tyrosine 1472 is required/involved in CaMKII binding and activation  and this phosphorylation site is linked to spinal pain transmission . Interestingly, 5-HT7 receptors agonists promote pain after formalin injection in animal models  however others have found anti-nociceptive effects of 5-HT7 receptors [37, 38]. These differences may be due to distinct central and peripheral pain pathways that both contain 5-HT7 receptors.
In summary, we have now identified two pathways downstream of the 5-HT7 receptor that ultimately regulate NMDA receptor activity/signaling (Figure 6). We have demonstrated that the upregulation of PDGFβ receptors by long-term treatment with 5-HT7 receptor agonists is sufficient to protect hippocampal neurons against NMDA excitotoxicity [16, 18] whereas acute activation of 5-HT7 receptors increased NMDA-evoked currents. These findings may help to explain why previous reports identified 5-HT7 receptors as both positive and negative regulators of NMDA receptor signaling.
Materials and methods
Reagents and antibodies
5-CT, LP 12 (4-(2-Diphenyl)-N-(1,2,3,4-tetrahydronaphthalen-1-yl)-1- piperazinehexanamide hydrochloride), and H89 were purchased from Sigma (St. Louis, MO, USA). The 5-HT7 receptor antagonists SB 258719 ((R)-3,N-Dimethyl-N-[1-methyl-3-(4-methylpiperidin-1-yl)propyl]benzene sulfonamide) and SB 269970 (R-3-(2-(2-(4-methylpiperidin-1-yl)ethyl)-pyrrolidine-1-sulfonyl)-phenol as well as Go 6983 were purchased from Tocris (Ellisville, MO, USA). Antibodies purchased from Millipore (Bellerica, MA) include anti-NR1, anti-phospho-896-NR1, anti-phospho-897-NR1, anti-NR2A, anti-phospho-pan-NR2A, anti-NR2B, anti-phospho-1252-NR2B, anti-phospho-1336-NR2B, and anti-phospho-1472-NR2B.
Cell isolation and whole-cell recording
CA1 neurons were isolated from hippocampal slices of postnatal day 14–21 Wistar rats as previously described . The extracellular solution was composed of 140 mM NaCl, 1.3 mM CaCl2, 25 mM N-2-hydroxyethylpiperazine-N’-ethanesulfonic acid (HEPES), 33 mM glucose, 5.4 mM KCl, and 0.5 μM tetrodotoxin, and 0.5 μM glycine, with pH of 7.3-7.4 and osmolarity ranging from 320–330 mOsm. Recordings were done at room temperature. The intracellular solution consisted of 11 mM ethyleneglycol-bis-(α-amino-ethyl ether) N,N’-tetra-acetic acid (EGTA) as intracellular calcium chelating buffer, 10 mM HEPES, 2 mM MgCl2, 2 mM tetraethyl ammonium chloride (TEA-Cl) to block K + channel, 1 mM CaCl2, 140 mM CsF, and 4 mM K2ATP. NMDA currents were evoked by rapid application of NMDA solution delivered from a multi-barreled fast perfusion system for 2 s every minute.
Hippocampal slices were prepared, treated with drugs for 5 min, and homogenized chilled lysis buffer (20 mM Tris–HCl at pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 30 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium orthovanadate, 0.5% SDS, and 1% triton-X-100; supplemented with Halt Protease and Phosphatase Inhibitor (Thermo, Fisher, Markham, Ontario)) prior to use. Lysates were centrifuged at 14,000 × g for 20 min at 4°C and the supernatant was collected. The supernatant was subjected to SDS-PAGE and proteins were transferred to nitrocellulose membranes, blocked with 5% non-fat dry milk in Tris-buffered saline and 0.1% Tween for 1 h at room temperature or overnight at 4°C, and incubated in primary antibodies for 1 h at room temperature or overnight at 4°C. Membranes were washed three times in Tris-buffered saline with 0.1% Tween-20, incubated with HRP-conjugated secondary antibodies for 1 h at room temperature, washed again, and bound antibodies were visualized by the enhanced chemiluminescence using the chemiluminescent substrate (Millipore, Etobicoke, Ontario). Images of Western blots were taken on the Kodak 4000 MM Pro Imaging Station, and densitometric analyses were performed using the Kodak Molecular Imaging software. Membranes were then stripped and reprobed with other antibodies.
Surface biotinylation assay
Hippocampal slices were incubated for 5 min with 5-HT7 receptor agonists and antagonists. Slices were washed in ice-cold ECF and incubated with 0.5 mg/ml Sulfo-NHS-LC-biotin (Pierce) for 30 min. The biotin reaction was quenched by washing with 10 mM glycine. Slices were washed twice more and homogenized in lysis buffer. Lysate protein concentrations were normalized and lysates were incubated with streptavidin beads overnight at 4°C (Sigma). Beads were washed three times in lysis buffer and boiled in loading buffer for 5 min before separation by SDS-PAGE.
Statistical analysis of the data was performed using the Prism® GraphPad program. For electrophysiology, graphs and sample tracings were made using the Origin® program. Significance level was set at α = 0.05. Data was analyzed by one-way ANOVA or Student’s t-test where appropriate.
All animal experiments were performed in agreement with the guidelines of the policies on the Use of Animals at the University of Waterloo (protocol #09-17) and the University of Western Ontario.
Ddorsal Raphe nucleus
Excitatory post-synaptic potentials
Platelet-derived growth factor
Paoletti P, Neyton J: NMDA receptor subunits: function and pharmacology. Curr Opin Pharmacol. 2007, 7: 39-47. 10.1016/j.coph.2006.08.011.
Al-Hallaq RA, Conrads TP, Veenstra TD, Wenthold RJ: NMDA di-heteromeric receptor populations and associated proteins in rat hippocampus. J Neurosci. 2007, 27: 8334-8343. 10.1523/JNEUROSCI.2155-07.2007.
Rauner C, Kohr G: Triheteromeric NR1/NR2A/NR2B receptors constitute the major N-methyl-D-aspartate receptor population in adult hippocampal synapses. J Biol Chem. 2011, 286: 7558-7566. 10.1074/jbc.M110.182600.
Yuen EY, Jiang Q, Chen P, Gu Z, Feng J, Yan Z: Serotonin 5-HT1A receptors regulate NMDA receptor channels through a microtubule-dependent mechanism. J Neurosci. 2005, 25: 5488-5501. 10.1523/JNEUROSCI.1187-05.2005.
Liang X, Arvanov VL, Wang RY: Inhibition of NMDA-receptor mediated response in the rat medial prefrontal cortical pyramidal cells by the 5-HT3 receptor agonist SR 57227A and 5-HT: intracellular studies. Synapse. 1998, 29: 257-268. 10.1002/(SICI)1098-2396(199807)29:3<257::AID-SYN8>3.0.CO;2-5.
Blank T, Zwart R, Nijholt I, Spiess J: Serotonin 5-HT2 receptor activation potentiates N-methyl-D-aspartate receptor-mediated ion currents by a protein kinase C-dependent mechanism. J Neurosci Res. 1996, 45: 153-160. 10.1002/(SICI)1097-4547(19960715)45:2<153::AID-JNR7>3.0.CO;2-9.
Arvanov VL, Liang X, Magro P, Roberts R, Wang RY: A pre- and postsynaptic modulatory action of 5-HT and the 5-HT2A, 2C receptor agonist DOB on NMDA-evoked responses in the rat medial prefrontal cortex. Eur J Neurosci. 1999, 11: 2917-2934. 10.1046/j.1460-9568.1999.00708.x.
Leopoldo M, Lacivita E, Berardi F, Perrone R, Hedlund PB: Serotonin 5-HT7 receptor agents: Structure-activity relationships and potential therapeutic applications in central nervous system disorders. Pharmacol Ther. 2011, 129: 120-148. 10.1016/j.pharmthera.2010.08.013.
Duncan MJ, Congleton MR: Neural mechanisms mediating circadian phase resetting by activation of 5-HT(7) receptors in the dorsal raphe: roles of GABAergic and glutamatergic neurotransmission. Brain Res. 2010, 1366: 110-119.
Harsing LG, Prauda I, Barkoczy J, Matyus P, Juranyi Z: A 5-HT7 heteroreceptor-mediated inhibition of [3H]serotonin release in raphe nuclei slices of the rat: evidence for a serotonergic-glutamatergic interaction. Neurochem Res. 2004, 29: 1487-1497.
Smith BN, Sollars PJ, Dudek FE, Pickard GE: Serotonergic modulation of retinal input to the mouse suprachiasmatic nucleus mediated by 5-HT1B and 5-HT7 receptors. J Biol Rhythms. 2001, 16: 25-38. 10.1177/074873040101600104.
Quintero JE, McMahon DG: Serotonin modulates glutamate responses in isolated suprachiasmatic nucleus neurons. J Neurophysiol. 1999, 82: 533-539.
Roberts AJ, Krucker T, Levy CL, Slanina KA, Sutcliffe JG, Hedlund PB: Mice lacking 5-HT receptors show specific impairments in contextual learning. Eur J Neurosci. 2004, 19: 1913-1922. 10.1111/j.1460-9568.2004.03288.x.
Crider JY, Williams GW, Drace CD, Katoli P, Senchyna M, Sharif NA: Pharmacological characterization of a serotonin receptor (5-HT7) stimulating cAMP production in human corneal epithelial cells. Invest Ophthalmol Vis Sci. 2003, 44: 4837-4844. 10.1167/iovs.02-1292.
Kvachnina E, Liu G, Dityatev A, Renner U, Dumuis A, Richter DW, Dityateva G, Schachner M, Voyno-Yasenetskaya TA, Ponimaskin EG: 5-HT7 receptor is coupled to G alpha subunits of heterotrimeric G12-protein to regulate gene transcription and neuronal morphology. J Neurosci. 2005, 25: 7821-7830. 10.1523/JNEUROSCI.1790-05.2005.
Vasefi MS, Kruk JS, Liu H, Heikkila JJ, Beazely MA: Activation of 5-HT7 receptors increases neuronal platelet-derived growth factor beta receptor expression. Neurosci Lett. 2012, 511: 65-69. 10.1016/j.neulet.2012.01.016.
Beazely MA, Lim A, Li H, Trepanier C, Chen X, Sidhu B, Macdonald JF: Platelet-derived growth factor selectively inhibits NR2B-containing N-methyl-D-aspartate receptors in CA1 hippocampal neurons. J Biol Chem. 2009, 284: 8054-8063. 10.1074/jbc.M805384200.
Vasefi MS, Kruk JS, Heikkila JJ, Beazely MA: 5-HT7 receptor neuroprotection against NMDA–induced excitotoxicity is PDGFß receptor-dependent. J Neurochem. 2013, 125: 26-36. 10.1111/jnc.12157.
Eglen RM, Jasper JR, Chang DJ, Martin GR: The 5-HT7 receptor: orphan found. Trends Pharmacol Sci. 1997, 18: 104-107. 10.1016/S0165-6147(97)01043-2.
Hoyer D, Clarke DE, Fozard JR, Hartig PR, Martin GR, Mylecharane EJ, Saxena PR, Humphrey PP: International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (Serotonin). Pharmacol Rev. 1994, 46: 157-203.
Mahe C, Bernhard M, Bobirnac I, Keser C, Loetscher E, Feuerbach D, Dev KK, Schoeffter P: Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines. Br J Pharmacol. 2004, 143: 404-410. 10.1038/sj.bjp.0705936.
Romero G, Pujol M, Pauwels PJ: Reanalysis of constitutively active rat and human 5-HT7(a) receptors in HEK-293F cells demonstrates lack of silent properties for reported neutral antagonists. Naunyn Schmiedebergs Arch Pharmacol. 2006, 374: 31-39. 10.1007/s00210-006-0093-y.
Andressen KW, Norum JH, Levy FO, Krobert KA: Activation of adenylyl cyclase by endogenous G(s)-coupled receptors in human embryonic kidney 293 cells is attenuated by 5-HT(7) receptor expression. Mol Pharmacol. 2006, 69: 207-215.
Krobert KA, Bach T, Syversveen T, Kvingedal AM, Levy FO: The cloned human 5-HT7 receptor splice variants: a comparative characterization of their pharmacology, function and distribution. Naunyn Schmiedebergs Arch Pharmacol. 2001, 363: 620-632. 10.1007/s002100000369.
Thomas EA, Cravatt BF, Sutcliffe JG: The endogenous lipid oleamide activates serotonin 5-HT7 neurons in mouse thalamus and hypothalamus. J Neurochem. 1999, 72: 2370-2378.
Leopoldo M, Lacivita E, Contino M, Colabufo NA, Berardi F, Perrone R: Structure-activity relationship study on N-(1,2,3,4-tetrahydronaphthalen-1-yl)-4-aryl-1-piperazinehexanamides, a class of 5-HT7 receptor agents. 2. J Med Chem. 2007, 50: 4214-4221. 10.1021/jm070487n.
Mahe C, Loetscher E, Feuerbach D, Muller W, Seiler MP, Schoeffter P: Differential inverse agonist efficacies of SB-258719, SB-258741 and SB-269970 at human recombinant serotonin 5-HT7 receptors. Eur J Pharmacol. 2004, 495: 97-102. 10.1016/j.ejphar.2004.05.033.
Bacon WL, Beck SG: 5-Hydroxytryptamine(7) receptor activation decreases slow afterhyperpolarization amplitude in CA3 hippocampal pyramidal cells. J Pharmacol Exp Ther. 2000, 294: 672-679.
Tokarski K, Zahorodna A, Bobula B, Hess G: 5-HT7 receptors increase the excitability of rat hippocampal CA1 pyramidal neurons. Brain Res. 2003, 993: 230-234. 10.1016/j.brainres.2003.09.015.
Valenzuela CF, Xiong Z, MacDonald JF, Weiner JL, Frazier CJ, Dunwiddie TV, Kazlauskas A, Whiting PJ, Harris RA: Platelet-derived growth factor induces a long-term inhibition of N-methyl-D-aspartate receptor function. J Biol Chem. 1996, 271: 16151-16159. 10.1074/jbc.271.27.16151.
Meneses A: Effects of the 5-HT7 receptor antagonists SB-269970 and DR 4004 in autoshaping Pavlovian/instrumental learning task. Behav Brain Res. 2004, 155: 275-282. 10.1016/j.bbr.2004.04.026.
Sarkisyan G, Hedlund PB: The 5-HT7 receptor is involved in allocentric spatial memory information processing. Behav Brain Res. 2009, 202: 26-31. 10.1016/j.bbr.2009.03.011.
Perez-Garcia GS, Meneses A: Effects of the potential 5-HT7 receptor agonist AS 19 in an autoshaping learning task. Behav Brain Res. 2005, 163: 136-140. 10.1016/j.bbr.2005.04.014.
Nakazawa T, Komai S, Watabe AM, Kiyama Y, Fukaya M, Arima-Yoshida F, Horai R, Sudo K, Ebine K, Delawary M: NR2B tyrosine phosphorylation modulates fear learning as well as amygdaloid synaptic plasticity. EMBO J. 2006, 25: 2867-2877. 10.1038/sj.emboj.7601156.
Katano T, Nakazawa T, Nakatsuka T, Watanabe M, Yamamoto T, Ito S: Involvement of spinal phosphorylation cascade of Tyr1472-NR2B, Thr286-CaMKII, and Ser831-GluR1 in neuropathic pain. Neuropharmacology. 2011, 60: 609-616. 10.1016/j.neuropharm.2010.12.005.
Rocha-Gonzalez HI, Meneses A, Carlton SM, Granados-Soto V: Pronociceptive role of peripheral and spinal 5-HT7 receptors in the formalin test. Pain. 2005, 117: 182-192. 10.1016/j.pain.2005.06.011.
Dogrul A, Seyrek M: Systemic morphine produce antinociception mediated by spinal 5-HT7, but not 5-HT1A and 5-HT2 receptors in the spinal cord. Br J Pharmacol. 2006, 149: 498-505. 10.1038/sj.bjp.0706854.
Brenchat A, Romero L, Garcia M, Pujol M, Burgueno J, Torrens A, Hamon M, Baeyens JM, Buschmann H, Zamanillo D, Vela JM: 5-HT7 receptor activation inhibits mechanical hypersensitivity secondary to capsaicin sensitization in mice. Pain. 2009, 141: 239-247. 10.1016/j.pain.2008.11.009.
Wang LY, MacDonald JF: Modulation by magnesium of the affinity of NMDA receptors for glycine in murine hippocampal neurones. J Physiol. 1995, 486 (Pt 1): 83-95.
Special thanks to Nancy Gibson and Dawn McCutcheon for their animal care and use of their facility while ours was under construction. This research was funded through generous start-up funding from the University of Waterloo, Faculty of Science and by the National Science and Engineering Research Council of Canada. The contributions of KY and JFM to this manuscript were supported by the Canadian Institutes of Health Research.
The authors declare that they have no competing interests.
MSV carried out the Western blot and biotinylation experiments and KY performed the electrophysiology experiments under the supervision of JFM and MFJ. JL and JSK contributed to the biochemical experimentation and participated in the drafting of the manuscript. JJH co-supervised MSV and JSK and participated in the drafting the manuscript. MAB conceived of the study, participated in its design and coordination, participated in the drafting of the manuscript, and supervised JL, MSV, and JSK. All authors read and approved the final manuscript.
Authors’ original submitted files for images
About this article
Cite this article
Vasefi, M.S., Yang, K., Li, J. et al. Acute 5-HT7 receptor activation increases NMDA-evoked currents and differentially alters NMDA receptor subunit phosphorylation and trafficking in hippocampal neurons. Mol Brain 6, 24 (2013). https://doi.org/10.1186/1756-6606-6-24
- Isolated neurons