- Short report
- Open Access
Block of T-type calcium channels by protoxins I and II
Molecular Brain volume 7, Article number: 36 (2014)
Low-voltage-activated (T-type) calcium channels play a crucial role in a number of physiological processes, including neuronal and cardiac pacemaker activity and nociception. Therefore, finding specific modulators and/or blockers of T-type channels has become an important field of drug discovery. One characteristic of T-type calcium channels is that they share several structural similarities with voltage-gated sodium channels (VGSCs). We therefore hypothesized that binding sites for certain sodium channel blocking peptide toxins may be present in T-type calcium channels.
The sodium channel blocker ProTx I tonically blocked native and transiently expressed T-type channels in the sub- to low micro molar range with at least a ten-fold selectivity for the T-type calcium channel hCav3.1 over hCav3.3, and more than one hundred fold selectivity over hCav3.2. Using chimeras of hCav3.1 and hCav3.3, we determined that the domain IV region of hCav3.1 is a major determinant of toxin affinity, with a minor contribution from domain II. Further analysis revealed several residues in a highly conserved region between T-type and sodium channels that may correspond to toxin binding sites. Mutagenesis of several of these residues on an individual basis, however, did not alter the blocking effects of the toxin. ProTx II on the other hand preferentially blocked hCav3.2 and significantly shifted the steady state inactivation of this channel.
ProTx I blocks hCav3.1 both selectively and with high affinity. Domain IV appears to play a major role in this selectivity with some contribution from domain II. Given the structural similarities between sodium and T-type calcium channels and the apparent conservation in toxin binding sites, these data could provide insights into the development and synthesis of novel T-type channel antagonists.
Low-voltage-activated (LVA) or “T-type” calcium channels are encoded by one of three different types of Cav3 α1 subunits (Cav3.1, Cav3.2 and Cav3.3, also known as α1G, α1H, and α1I, respectively) whose membrane topology is similar to those of sodium channels . They are activated by small membrane depolarizations and display rapid activation and inactivation kinetics  and they are responsible for triggering low-threshold depolarizations that in turn lead to the initiation of action potentials. Consequently, they are thought to be important for regulating neuronal and cardiac pacemaker activity, and disruption of their normal activity can contribute to cardiac hypertrophy [3–5] and neuronal hyperexcitabilty disorders such as epilepsy and pain [6–9]. Similarly, mutations in Cav3.2 T-type calcium channels have been linked to absence seizures [6, 7] and up-regulation of Cav3.2 T-type channel activity in primary afferent fibers has been linked to the development of chronic pain [8, 9]. Indeed, depletion of Cav3.2 results in hyposensitivity to pain [10, 11].
Modulators and blockers of T-type calcium channels may be useful in elucidating the exact role of these channels in cell signaling pathways and may be exploited for therapeutic purposes. Identification of drugs and molecules that selectively interact with T-type calcium channels has, however, so far proven difficult, although recently novel small organic scaffolds for T-type channel inhibitors have been derived from blockers of other calcium channel subtypes, such as L-type channels [12–14].
Another class of molecules that are known to be effective blockers of voltage gated ion channels are polypeptide toxins. The effects of toxins on ion channels have been extensively documented [for review see Catterall et al.,  and one toxin isolated from scorpion venom (kurtoxin) is known to be a potent blocker of T-type calcium channels . This toxin however has been shown to also block high-voltage activated calcium channels . More recently, two peptide toxins (ProTx I and ProTx II), isolated from Tarantula venom, have been shown to be potent blockers of both sodium and calcium channels [18–20]. Given the structural similarities between these two classes of ion channels, we tested to what extent these two toxins inhibited T-type calcium channels and identified channel structural determinants of toxin block.
Our results reveal that ProTx II selectively blocks human Cav3.2 (hCav3.2) albeit with far less efficacy than previous reports suggested . ProTx I on the other hand potently and preferentially blocks human Cav3.1 (hCav3.1) in the sub-micro molar range. We therefore focused on ProTx I and used chimeras of hCav3.1 and hCav3.3, as well as sequence alignment between T-type and Nav channels and toxin interaction sites, to determine that Domain IV of hCav3.1 and, to a lesser extent Domain II, are key toxin interaction regions.
Results and discussion
ProTx II is a preferential blocker of hCav3.2
ProTx II was originally identified as potent inhibitor of sodium channels with fifteen to hundred- fold selectivity for hNav1.7 versus other sodium channels [18, 19]. It was also reported to be a potent inhibitor of L-type calcium channels and to mediate a greater degree of inhibition of Cav3.1 versus Cav3.2 . Our observations indicate that ProTx II in fact blocks hCav3.2 more potently than the other T-type calcium channels. The toxin blocked endogenous Cav3.2 channels in acutely isolated mouse DRG neurons  with similar potency [Figure 1]. ProTx II also dramatically shifts the steady state inactivation of hCav3.2 towards more hyperpolarized potentials [Table 1A] which produces additional inhibition at typical neuronal resting membrane potentials. Interestingly, although ProTx II only weakly blocked hCav3.1 and hCav3.3, there were significant negative shifts in the half activation potentials of these channels in the presence of as little as 1 μM of the toxin [Table 1A]. Together, these data indicate that ProTx II interacts with hCav3.2 channels in a way similar to β-scorpion toxin interactions with sodium channels , and that it also modulates the gating behavior of hCav3.1 and hCav3.3 channels.
ProTx I is both a potent and selective blocker of hCav3.1
ProTx I belongs to the inhibitory cysteine knot (ICK) family of peptide toxins that are known to interact with voltage-gated ion channels [18, 22] and to be potent inhibitors of voltage-gated sodium channels [18–20, 23]. More recently, several studies demonstrated that ProTx I could also potently block the human Cav3.1 calcium channel [18, 24]. One of these studies showed that ProTx I was selective for hCav3.1 over hCav3.2 and that this selectivity may be in part attributed to the S3-S4 linker in Domain IV of hCav3. . In our hands, when applied to transiently expressed hCav3 channels, ProTx I preferentially inhibited hCav3.1 channels, with less block of hCav3.3 and only very little inhibitory effect on hCav3.2. We tested 1 μM ProTx I on endogenous Cav3.1 that comprise a large portion of T-type current in isolated mouse thalamic neurons , and observed inhibition that was similar to that of hCav3.1 [Figure 1, Table 1B]. Contrary to previous findings, we did not observe any significant positive shifts in the half activation potential of hCav3.1 in the presence of 1 μM ProTx I in spite of its potent inhibitory effects, nor was the gating of hCav3.3 channels affected [Figure 2A,C and Table 1B]. In contrast, hCav3.2 channels underwent shifts in both the half-activation and inactivation potential in the presence of the toxin [Figure 2B, Table 1B].
The domain IV region of hCav3.1 is important for ProTx I block and function of hCav3.1
We had previously constructed a series of chimeric channels in which we had swapped various membrane domains between Cav3.1 and Cav3.3 . We used a subset of these chimeras to ascertain which of the membrane domains were responsible for the differences in ProTx I blocking effects on these two channel subtypes. As shown in Figure 3, chimeras that contained the Domain IV region of hCav3.1 exhibited a degree of block that was similar in magnitude to that of the wild type channel, whereas constructs that contained Cav3.3 sequence in this domain behaved liked the wild type Cav3.3 channels [Figure 3]. These data indicate that Domain IV is a major determinant of ProTx I action on T-type calcium channels. In addition, replacing domain II in the IGIG chimera with corresponding Cav3.3 sequence (IIIG) weakened the blocking effect, suggesting that domain II may also contribute to toxin action. We also examined the effect of the toxin on the gating behavior of these sets of chimeras. Most of the chimeras did not undergo a toxin induced change in half-activation or inactivation potential as expected from our observation with wild type channels, although two constructs exhibited a minor depolarizing shift in half activation potential when the toxin was applied [Table 2]. We note that a previous study in which ProTx I was shown to induce a dramatic shift in half activation potential of Cav3.1 used a cDNA derived from rat . We therefore tested ProTx I on rat Cav3.1 and although there was a slight positive shift in activation, it did not reach significance. We did, however, observe a small but significant negative shift in the steady state inactivation that was consistent with previous findings  and which curiously contrasts with our observation with human Cav3.1 where no significant shift was observed [Table 2].
Substitution of individual amino acid residues in the putative toxin blocking sites do not affect ProTx I block of hCav3.1
Given the high degree of homology between T-type calcium channels, we used ClustalW2 multiple sequence alignment of both the Domain II and Domain IV regions of these channels to determine if there were any sites that were unique to hCav3.1 that might be involved in toxin block, taking into consideration the loci of amino acid residues that are known to be involved in toxin block of voltage-gated sodium channels [27–29]. Our alignment results (Figure 4A) yielded nine candidate residues, two in Domain II of hCav3.1 and seven in Domain IV. To determine whether these residues may be involved in ProTx I block of hCav3.1, we replaced all nine residues with corresponding residues in Cav3.3, and then assessed ProTx I block of these mutant channels.
As shown in Figure 4B and Table 3, three of the amino acid residues resulted in non-functional channels. Surface biotinylation experiments revealed that these mutant channels were trafficked appropriately to the cell surface (data not shown), indicating that their inability to support whole cell currents was not due to an absence of expression. Instead, since these substitutions are in the region thought to be involved in inactivation, it is likely that these substitutions disrupted this mechanism. Of the remaining mutations, none resulted in a significant decrease in ProTx I block. Two of the mutations V1618A and Q1631K both displayed a significant negative shift in their half inactivation potential compared to wild type channels. In addition, the V1618A mutant also showed a significant positive shift in half activation potential compared to wild type [Table 3A]. These data indicate that although individual candidate amino acid residues cannot account for the blocking effects of this toxin, these residues do play a minor role in regulating the gating behavior of the channel.
Comparison with previous work
Previous studies have shown that the tarantula venom peptides ProTx I and ProTx II inhibit voltage-gated sodium channels by shifting their voltage dependence of activation to more positive potentials [18, 20]. Our results show that ProTx I preferentially blocked hCav3.1 at sub-micro molar concentrations, but we did not observe any shift in half activation potential. Contrary to previous findings , ProTx II appeared to preferentially block hCav3.2. This toxin block caused a significant negative shift in half inactivation voltage of hCav3.2, but in contrast with previous studies on sodium and calcium channels, no significant change in half activation potential [18, 30, 31]. The apparent differences between some of our results and those of previous studies, may be in part be due to the different expression systems, recording methods and clones used. In previous studies, HEK cells and Xenopus oocytes were used to express rat Cav3 channels and toxin effect and channel kinetics were measured on tail currents as an indicator of potency. We attempted to address this discrepancy by using ProTx I on a rat Cav3.1 clone available to us and although our results showed a small positive shift in the voltage-dependence of activation [Table 2], it did not reach significance. Further experiments will need to be conducted to determine the precise biophysical interactions of this toxin with T-type calcium channels, and how toxin actions are affected by different experimental conditions.
Our data show that ProTx I and ProTx II potently and preferentially block hCav3.1 and hCav3.2 respectively. These two toxins block and modify T-type calcium channels using mechanisms similar to their interaction with sodium channels [18, 20]. Their effect on the voltage dependence of inactivation is reminiscent of β-scorpion toxin interactions with sodium channels . Overall, our data suggest that both ProTx I and ProTx II may be useful towards exploring the gating mechanisms of T-type calcium channels. Finally, the apparent similarities in the toxin binding sites between Nav and Cav channels may provide an insight into the synthesis of more potent antagonists that act on either or both of these channel subtypes.
Materials and methods
Human Cav3.2 cDNA was kindly provided by Dr. Terrance Snutch (University of British Columbia, Vancouver, Canada). Human Cav3.3 was obtained from Dr. Arnaud Monteil (CNRS Montpellier, France), human Cav3.1 was described previously by our laboratory  and human Cav3.1 and Cav3.3 chimeras were also described previously .
Unless stated otherwise, chemicals were purchased from Sigma (St. Louis, MO). Both ProTx I and ProTx II were purchased from Alomone Labs (Jerusalem, Israel) and were dissolved in external recording solution at the stock concentration of 1 mM. All subsequent dilutions were also made in external recording solution.
tsA-201 cell culture and transfection
Human embryonic kidney tsA-201 cells were cultured and transfected using the calcium phosphate method as described previously . Briefly, 6 μg of T-type calcium channel Cav3.1, Cav3.2, and Cav3.3, α1 subunits were transfected together with 0.5 μg Enhanced green fluorescent protein (EGFP) DNA (Clontech) as a marker. Cells were re-suspended with 0.25% (w/v) trypsin-EDTA (Invitrogen) and plated on glass cover slips a minimum of 3 to 4 hours before patching and kept at 37°C and 5% CO2.
Isolation of neurons
Thalamic neurons were isolated as described previously . Briefly, thalami of adult mice were dissected out, cut into small pieces and then digested in papain (Worthington, LS003126) containing culture media. After digestion, the tissue was washed and triturated for neuron dissociation. Thalamic neurons were then seeded at low density onto coverslips pretreated with poly-d-lysine (Sigma, P7280). Dorsal Root Ganglia (DRG) neurons were isolated as described previously . Briefly, DRG from adult mice were removed and placed in Ca2+ and Mg2+-free Hank’s Balanced Salt Solution, containing (in mM): 140 NaCl, 5.3 KCl, 0.4 KH2PO4, 0.3 Na2HPO4, 6 D-glucose, 10 HEPES, and 2 mg/mL collagenase (Type I, Worthington, Lakewood, New Jersey), and 200 units of DNaseI (Worthington, Lakewood, New Jersey). Ganglia were then incubated for 45 min at 37°C and subsequently placed in media supplemented with 10% fetal bovine serum to stop digestion. Cells were then dispersed with fire polished Pasteur pipettes and plated on glass coverslips coated with 100 μg/mL poly-L-lysine.
Whole-cell voltage-clamp recordings on tsA- 201 cells were performed at room temperature 2 to 3 days after transfection. Whole-cell voltage-clamp recordings on Neuronal cells were performed at room temperature, the following day after isolation. The external recording solution for all calcium channel recordings contained (in mM): 114 CsCl, 20 BaCl2, 1 MgCl2, 10 HEPES, 10 Glucose, adjusted to pH 7.4 with CsOH. For voltage-clamp recordings on neuronal cells, 5 μM CdCl2 was also added to the external solution to inhibit high voltage activated calcium channels. For all recordings, the internal patch pipette solution contained [in mM]: 108 CsMeSO4, 2 MgCl2, 11 EGTA, 10 HEPES adjusted to pH 7.3 with CsOH. The internal solution was supplemented with 0.6 mM GTP and 2 mM ATP, which were added directly to the internal solution immediately before use. Liquid junction potentials for the above solutions were left uncorrected. Recordings were digitized at 5 kHz and low-pass filtered at 1 kHz.
Toxins were prepared daily in external solution and applied locally to cells with the use of a custom built gravity driven micro-perfusion system that exchanges solution in approximately one second . Currents were elicited from a holding potential of −110 mV and were measured by conventional whole-cell patch clamp using an Axopatch 200B amplifier in combination with Clampex 9.2 software (Molecular Devices, Sunnyvale, CA). After establishment of the whole cell configuration, cellular capacitance was minimized using the analog compensation available on the amplifier. Series resistance was <10 MΩ and was compensated >85% in all experiments. Data were filtered at 1 kHz (8-pole Bessel) and digitized at 10 kHz with a Digidata 1320 interface (Molecular Devices). In addition to collecting the raw data, an online leak-subtraction protocol was used in which four pulses of opposite polarity and one-quarter amplitude were applied immediately before the test protocol. For current–voltage relation studies, the membrane potential was held at −100 mV and cells were depolarized from −80 to 20 mV in 10 mV increments. For steady-state inactivation studies, the membrane potential was depolarized by test pulses to −20 mV after 3.6-s conditioning pre-pulses ranging from −110 to −20 mV. The current amplitude obtained from each test pulse was then normalized to that observed at a holding potential of −110 mV.
Data analysis and statistics
Data were analyzed using Clampfit 9.2 (Molecular Devices). Preparation of figures and curve fitting was carried out with Origin 7.5 software (Northampton, MA, USA). Current–voltage relationships were fitted with the modified Boltzmann equation: I = (Gmax*(Vm-Erev))/(1 + exp((V0.5act-Vm)/ka)), where Vm is the test potential, V0.5act is the half-activation potential, Erev is the reversal potential, Gmax is the maximum slope conductance, and ka reflects the slope of the activation curve. Data from concentration-dependence studies were fitted with the equation y = A2 + (A1-A2)/(1 + ([C]/IC50)P) where A1 is initial current amplitude and A2 is the current amplitude at saturating drug concentrations, [C] is the drug concentration and P is the Hill coefficient. Statistical significance was determined by paired or unpaired Student’s t-Tests and one-way or repeated measures ANOVA followed by Tukey’s multiple comparison tests. Significant values were set as indicated in the text and figure legends. All data are given as means +/− standard errors. Steady-state inactivation curves were fitted using the Boltzmann equation: I = 1/(1 + exp((Vm-V h)/k)), where V h is the half-inactivation potential and k is the slope factor.
All experiments performed in this manuscript comply with the laws of Canada.
- ProTx I and II:
Protoxin I and II
Voltage gated calcium channel
Human voltage activated calcium channel
Voltage activated sodium channel
Half activation potential
Dorsal root ganglion.
Catterall WA, Perez-Reyes E, Snutch TP, Striessnig J: International union of pharmacology. XLVIII. Nomenclature and structure-function relationships of voltage-gated calcium channels. Pharmacol Rev. 2005, 57 (4): 411-25. 10.1124/pr.57.4.5.
Perez-Reyes E: Molecular physiology of low-voltage-activated t-type calcium channels. Physiol Rev. 2003, 83: 117-161.
Bender KJ, Uebele VN, Renger JJ, Trussell LO: Control of firing patterns through modulation of axon initial segment T-type calcium channels. J Physiol. 2012, 590: 109-118.
Cain SM, Snutch TP: Contributions of T-type calcium channel isoforms to neuronal firing. Channels. 2010, 4: 475-482.
Cribbs L: T-type calcium channel expression and function in the diseased heart. Channels. 2010, 4 (6): 447-52.
Heron SE, Khosravani H, Varela D, Bladen C, Williams TC, Newman MR, Scheffer IE, Berkovic SF, Mulley JC, Zamponi GW: Extended spectrum of idiopathic generalized epilepsies associated with cacna1h functional variants. Ann Neurol. 2007, 62: 560-568. 10.1002/ana.21169.
Khosravani H, Zamponi GW: Voltage-gated calcium channels and idiopathic generalized epilepsies. Physiol Rev. 2006, 86: 941-966. 10.1152/physrev.00002.2006.
Altier C, Zamponi GW: Targeting Ca2+ channels to treat pain: T-type versus N-type. Trends Pharmacol Sci. 2004, 25: 465-470. 10.1016/j.tips.2004.07.004.
Park J, Luo ZD: Calcium channel functions in pain processing. Channels. 2010, 4 (6): 510-7.
Wang W, Gu J, Li YQ, Tao YX: Are voltage-gated sodium channels on the dorsal root ganglion involved in the development of neuropathic pain?. Mol Pain. 2011, 7: 16-10.1186/1744-8069-7-16.
Cregg R, Momin A, Rugiero F, Wood JN, Zhao J: Pain channelopathies. J Physiol. 2010, 588 (11): 1897-1904. 10.1113/jphysiol.2010.187807.
Lory P, Chemin J: Towards the discovery of novel T-type calcium channel blockers. Expert Opin Ther Targets. 2007, 11 (5): 717-722. 10.1517/1472818.104.22.1687.
Bladen C, Gündüz MG, Simşek R, Safak C, Zamponi GW: Synthesis and evaluation of 1,4-dihydropyridine derivatives with calcium channel blocking activity. Pflugers Arch. 2013, In press. doi:10.1007/s00424-013-1376-z
Kumar PP, Stotz SC, Paramashivappa R, Beedle AM, Zamponi GW, Srinivasa A: Synthesis and evaluation of a new class of nifedipine analogs with t-type calcium channel blocking activity. Mol Pharmacol. 2002, 61: 649-658. 10.1124/mol.61.3.649.
Catterall WA, Cestèle S, Yarov-Yarovoy V, Yu FH, Konoki K, Scheuer T: Voltage-gated ion channels and gating modifier toxins. Toxicon. 2007, 49 (2): 124-41. 10.1016/j.toxicon.2006.09.022.
Chuang RS, Jaffe H, Cribbs L, Perez-Reyes E, Swartz KJ: Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin. Nat Neurosci. 1998, 1: 668-674. 10.1038/3669.
Sidach SS, Mintz IM: Kurtoxin, a gating modifier of neuronal high- and low-threshold ca channels. J Neurosci. 2002, 22: 2023-2034.
Middleton RE, Warren VA, Kraus RL, Hwang JC, Liu CJ, Dai G, Brochu RM, Kohler MG, Gao YD, Garsky VM, Bogusky MJ, Mehl JT, Cohen CJ, Smith MM: Two tarantula peptides inhibit activation of multiple sodium channels. Biochemistry. 2002, 41 (50): 14734-47. 10.1021/bi026546a.
Xiao Y, Blumenthal K, Jackson JO, Liang S, Cummins TR: The tarantula toxins ProTx-II and huwentoxin-IV differentially interact with human Nav1.7 voltage sensors to inhibit channel activation and inactivation. Mol Pharmacol. 2010, 78 (6): 1124-34. 10.1124/mol.110.066332.
Priest BT, Blumenthal KM, Smith JJ, Warren VA, Smith MM: ProTx-I and ProTx-II: gating modifiers of voltage-gated sodium channels. Toxicon. 2007, 49 (2): 194-201. 10.1016/j.toxicon.2006.09.014.
Hildebrand ME, Smith PL, Bladen C, Eduljeea C, Xie JY, Chen L, Fee-Maki M, Doering CJ, Mezeyova J, Zhu Y, Belardetti F, Pajouhesh H, Parker D, Arneric SP, Parmar M, Porreca F, Tringham E, Zamponi GW, Snutch TP: A novel slow-inactivation-specificion channel modulator attenuates neuropathic pain. Pain. 2010, 152 (4): 833-843.
Gui J, Liu B, Cao G, Lipchik AM, Perez M, Dekan Z, Mobli M, Daly NL, Alewood PF, Parker LL, King G, Zhou Y, Jordt SE, Nitabach MN: A tarantula-venom peptide antagonizes the trpa1 nociceptor ion channel by binding to the s1-s4 gating domain. Curr Biol. 2014, S0960-9822 (14): 00014-1. [Epub ahead of print]
Bosmans F, Rash L, Zhu SY, Diochot S, Lazdunski M, Escoubas P, Tytgat T: Four novel tarantula toxins as selective modulators of voltage-gated sodium channel subtypes. Mol Pharmacol. 2006, 69 (2): 419-429.
Ohkubo T, Yamazaki J, Kitamura K: Tarantula toxin ProTx-I differentiates between human T-type voltage-gated Ca2+ Channels Cav3.1 and Cav3.2. J Pharmacol Sci. 2010, 112: 452-458. 10.1254/jphs.09356FP.
Kim D, Park D, Choi S, Lee S, Sun M, Kim C, Shin HS: Thalamic control of visceral nociception mediated by t-type ca2+ channels. Science. 2003, 302: 117-9. 10.1126/science.1088886.
Hamid J, Peloquin JB, Monteil A, Zamponi GW: Determinants of the differential gating properties of Cav3.1 and Cav3.3 T-type channels: a role of domain IV?. Neuroscience. 2006, 143 (3): 717-28. 10.1016/j.neuroscience.2006.08.023. 22
Rogers JC, Qu Y, Tanada TN, Scheuer T, Catterall WA: Molecular determinants of high affinity binding of alpha-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na + channel alpha subunit. J Biol Chem. 1996, 271: 15950-15962. 10.1074/jbc.271.27.15950.
Wang J, Yarov-Yarovoy V, Kahn R, Gordon D, Gurevitz M, Scheuer T, Catterall WA: Mapping the receptor site for α-scorpion toxins on a Na + channel voltage sensor. Proc Natl Acad Sci U S A. 2011, 108: 15426-15431. 10.1073/pnas.1112320108.
Gurevitz M: Mapping of scorpion toxin receptor sites at voltage-gated sodium channels. Toxicon. 2012, 60 (4): 502-11. 10.1016/j.toxicon.2012.03.022.
Smith JJ, Cummins TR, Alphy S, Blumenthal KM: Molecular interactions of the gating modifier toxin ProTx-II with NaV 1.5: implied existence of a novel toxin binding site coupled to activation. J Biol Chem. 2007, 282 (17): 12687-97. 10.1074/jbc.M610462200.
Edgerton GB, Blumenthal KM, Hanck DA: Inhibition of the activation pathway of the T-type calcium channel Ca(V)3.1 by ProTx II. Toxicon. 2010, 56 (4): 624-36. 10.1016/j.toxicon.2010.06.009.
Beedle AM, Hamid J, Zamponi GW: Inhibition of transiently expressed low- and high-voltage-activated calcium channels by trivalent metal cations. J Membr Biol. 2002, 187: 225-238. 10.1007/s00232-001-0166-2.
Altier C, Khosravani H, Evans RM, Hameed S, Peloquin JB, Vartian BA, Chen L, Beedle AM, Ferguson SS, Mezghrani A, Dubel SJ, Bourinet E, McRory JE, Zamponi GW: ORL1 receptor-mediated internalization of N-type calcium channels. Nat Neurosci. 2006, 9: 31-40. 10.1038/nn1605.
Weiss N, Hameed S, Fernández-Fernández JM, Fablet K, Karmazinova M, Poillot C, Proft J, Chen L, Bidaud I, Monteil A, Huc-Brandt S, Lacinova L, Lory P, Zamponi GW, De Waard M: A Ca(v)3.2/syntaxin-1A signaling complex controls T-type channel activity and low-threshold exocytosis. J Biol Chem. 2012, 287 (4): 2810-8. 10.1074/jbc.M111.290882.
Feng ZP, Doering CJ, Winkfein RJ, Beedle AM, Spafford JD, Zamponi GW: Determinants of inhibition of transiently expressed voltage-gated calcium channels by ω-conotoxins GVIA and MVIIA. J Biol Chem. 2003, 2003 (278): 20171-20178.
This work was supported by a grant from the Canadian Institutes of Health Research. CB holds a studentship award from AIHS. IAS holds a Mitacs Elevate fellowship. GWZ is a Canada Research Chair and an Alberta Innovates-Health Solutions (AIHS) scientist.
The authors declare that they have no competing interest.
CB designed and carried out electrophysiology experiments, point mutations and drafted the manuscript. JH designed and created hCav3.1-hCav3.3 chimeric channels. IAS designed and conducted biochemistry experiments. GWZ directed the study and edited the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Bladen, C., Hamid, J., Souza, I.A. et al. Block of T-type calcium channels by protoxins I and II. Mol Brain 7, 36 (2014). https://doi.org/10.1186/1756-6606-7-36
- Calcium channels
- ProTx I
- ProTx II
- T-type blockers