Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis
© The Author(s). 2017
Received: 7 June 2017
Accepted: 26 September 2017
Published: 5 October 2017
Chondroitin sulfate (CS) is an important glycosaminoglycan and is mainly found in the extracellular matrix as CS proteoglycans. In the brain, CS proteoglycans are highly concentrated in perineuronal nets (PNNs), which surround synapses and modulate their functions. To investigate the importance of CS, we produced and precisely examined mice that were deficient in the CS synthesizing enzyme, CSGalNAcT1 (T1KO). Biochemical analysis of T1KO revealed that loss of this enzyme reduced the amount of CS by approximately 50% in various brain regions. The amount of CS in PNNs was also diminished in T1KO compared to wild-type mice, although the amount of a major CS proteoglycan core protein, aggrecan, was not changed. In T1KO, we observed abnormalities in several behavioral tests, including the open-field test, acoustic startle response, and social preference. These results suggest that T1 is important for plasticity, probably due to regulation of CS-dependent PNNs, and that T1KO is a good model for investigation of PNNs.
Chondroitin sulfate (CS) is one of the most abundant glycosaminoglycans, which are composed of long, repeated disaccharide chains. For synthesis of CS proteoglycans (CSPGs), CS is attached to core proteins, such as aggrecan (AGR), neurocan, phosphacan, and versican. These CSPGs are mainly localized in the extracellular matrix. In the central nervous system, CSPG-enriched areas are called perineuronal nets (PNNs), which are specialized structures that surround synapses and are specifically recognized by Wisteria floribunda lectin (WFA). PNNs modulate synaptic functions, particularly GABAergic inhibitory input via parvalbumin (PV) (+) neuronal cells . Recently, the function of PNNs in the regulation of synaptic plasticity and memory [2, 3] has been investigated, and PNN abnormalities in relation to human mental diseases have been reported [4–6].
CS synthesis is sequentially performed by approximately 15 enzymes including glycosyltransferases and sulfotransferases in three steps: (I) tetrasaccharide linker synthesis attached to the core protein; (II) disaccharide repeat synthesis for GalNAc (N-acetylgalactosamine)-GlcUA (glucuronic acid); and (III) sulfation of these sugars. The first step is shared with another glycosaminoglycan, heparan sulfate; thus, the first unique step in CS synthesis is GalNAc transfer to the linker. CSGalNAcT1 (T1) is a key enzyme in CS synthesis, because this enzyme catalyzes the first unique step in CS synthesis, apart from heparan sulfate synthesis.
We previously produced T1 knockout mice (T1KO; [CSGalNAcT1−/−] mice) and examined some functions of CS . T1KO mice have impaired bone development and a 10% smaller body size than wild-type (WT) mice [7, 8]. They have the striking feature of rapid recovery following spinal cord injury . During recovery from spinal cord injury in T1KO, we observed a loss of PNNs in the spinal cord , suggesting abnormal PNN formation.
Here, we systematically examined the T1KO brain. Biochemically, the amount of CS was reduced by about half in each region in T1KO brain. Histologically, abnormal PNNs due to reduced CS were observed, and the mice demonstrated abnormal behaviors in several tests. Taken together, we conclude that T1 plays an important role in supplying CS for PNN development and brain functions related to several characteristic behaviors.
Biochemical and histochemical characteristics of T1KO
The composition of CS disaccharides in T1KO compared with WT
We quantitatively analyzed WFA, anti-AGR, and anti-PV immunofluorescence by assessing the intensity (Fig. 2i-p) following triple fluorescent staining. Somatosensory cortex showed a reduction in WFA (+) PNNs (Fig. 2i and m), but the intensities of anti-AGR and anti-PV immunofluorescence, which is thought to represent the amounts of AGR in PNNs and PV (+) cells, respectively, were not different in T1KO (Fig. 2m). We observed similar patterns in the cingulate cortex (Fig. 2j and n), hippocampus (Fig. 2k and o), and thalamic reticular nucleus of the T1KO (Fig. 2l and p). These results suggest that T1KO have reduced CS in PNNs but the core proteins remain, independent of CS.
Behavioral abnormalities in T1KO
The behavioral tests in T1KO that were not significantly different between genotypes (p ≥ 0.05)
General health/neurological screen
body temperature (°C)
grip strength (n)
wire hang (latency to fall, s)
latency to light (s)
light distance (cm)
dark distance (cm)
number of transitions
stay time in light (s)
center time (s)
Elevated plus maze
number of total entry (times)
entry into open arms (%)
total distance travelled (cm)
time on open arms (times)
Social interaction (novel environment)
duration of contacts (s)
number or contacts (times)
mean duration of contacts (s)
distance travelled (cm)
Porsolt forced swim
day1 immobility (%)
day2 immobility (%)
front swing/stride duration
front brake/stride duration
front propel/stride duration
front stance width
front step angle
front paw angle
hind brake/stride duration
hind step angle
hind paw angle
time spent around target (probe test, 1 day)
time spent around target (probe test, 1 month)
time spent around target (reversal test, 1 day)
Cued and contextual fear conditioning
conditioning (freezing, %)
context test, 1 day (freezing, %)
cued test, with tone,1 day (freezing, %)
context test, 30 days (freezing, %)
cued test, pretone period, 30 days (freezing, %)
cued test, with tone, 30 days (freezing, %)
24 h home cage monitoring
activity light period (A.U.)
activity dark period (A.U.)
number of particle dark period
Empty or Familiar
p value (paired-t)
Social approach test (Stranger vs Empty)
number of entry around cage (controls)
time spent around cage (mutants)
Social novelty preference test (Stranger vs Familiar)
number of entry around cage (controls)
number of entry around cage (mutants)
time spent around cage (controls)
time spent around cage (mutants)
Here, we showed both biochemical (Fig. 1; Table 1) and histochemical results (Figs. 2 and 3) demonstrating reduced CS in various areas of the T1KO brain. Histochemical results also revealed that the CS reduction was in PNNs of the various T1KO regions in brain, in the absence of a change in the amount of AGR (Figs. 2 and 3). In addition, several types of mouse behaviors were significantly abnormal in T1KO (Fig. 4), suggesting that these tests may reflect CS- and PNN-dependent changes. Regarding the CSPG species, we should consider membrane-bound proteins, such as receptor protein phosphatases . However, the total amount of CSPG species is likely considerably less than those of PNNs, considering the large space occupied by the extracellular matrix. Thus, our demonstration that T1KO have reduced PNNs is important.
Reductions in CS by at least 50% were observed in various T1KO brain regions, suggesting that T1 is an important enzyme responsible for CS synthesis (Fig. 1; Table 1), although five other enzymes with similar substrate specificities were reported . As far as we know, this is the first example of genetically targeted mice that showed a 50% loss in the total amount of CS. These results support the hypothesis that T1 is the most important enzyme for CS synthesis in the brain.
In addition, the CS-E ratios slightly but significantly increased in cerebrum and diencephalon of T1KO (Table 1). The exact reason of this change is not clearly known, however, it is likely that loss of T1 affects the following sulfation processes by sulfatases . In PNNs, CS-E is reported to be involved in association of semaphorin-3A , not only quantitative decrease but also such qualitative changes of PNNs may have some effects on the behavioral abnormalities (Fig. 4).
We also showed that T1KO generally had reduced WFA (+) PNNs in various brain areas, although the decrease was somewhat variable (Figs. 2 and 3). Thus, T1-dependent synthesis of CS is more prevalent in PNNs. In such cases, we did not detected abnormalities of the typical CSPG core protein, AGR, or another glycosaminoglycan, hyaluronic. These results suggest selective reduction of CS chains in PNNs in T1KO.
T1 enzyme deficiency caused behavioral abnormalities including abnormalities in the open field test, social interaction test, and acoustic startle response (Fig. 4). These results suggest that CS reduction in PNNs by T1 loss caused abnormalities in higher brain functions and hyperlocomotive activity in mice. These effects are thought to be due to an increase in excitability induced by impaired GABAergic functions . In addition, Ito et al. reported that chronic stress inhibits GABAergic signaling . Thus, although the exact relationship between each behavioral abnormality and reduced CS in PNNs is not fully understood, one possibility is that the balance in excitatory (glutamatergic) and inhibitory (GABAergic) inputs is shifted in abnormal PNNs, because in various regions, the functions of PV(+) cells which are known to be GABAergic interneurons, are regulated by PNN activity .
It is also recently reported that decreased GABAergic functions induce the sociability , which may be involved in the changes of our results concerning social interaction and preference (Fig. 4e-f), as models of human autism-spectrum and schizophrenia [15–17].
In our adult T1KO, fear conditioning memories were not significantly impaired (Fig. 4), in contrast to a previous report in which loss of CS in PNNs by chondroitin’s ABC (ChABC) erased these memories in mice younger than 3 weeks . Such a “critical period” in mice may have different mechanisms in adult vs. juvenile mice . Using these same T1KO, we recently showed that ocular dominance plasticity in the critical period was severely impaired after monocular deprivation, probably due to reduced function of PV (+) cells in the visual cortex . In mice aged 3 weeks for these experiments, AGR and PV (+) cells were reduced, together with CS in PNNs in the visual cortex , which is different from our current results in adult mice (Figs. 2 and 3). As shown in Figs. 2 and 3, some of these morphological abnormalities were observed and some were not. Taken together, T1KO may have additional behavioral abnormalities in the developmental stage, and may be a good model for studies of PNN-dependent neural plasticity. A report that the good results of open filed tests as higher brain functions, after learning, are reported to induce the increase of PNNs , seems related to our results (Fig. 4c).
Consistent with the above mention of chronic stress , PNNs may be also involved in human psychiatric disorders , and this idea should be confirmed using an appropriate animal model for PNNs. However, until now, only the ChABC-treated mouse model represented decreased PNNs. The problem with this acute model for inducing PNN abnormalities is that the extent of the decreases in CS in PNNs and how long this effect is maintained are unknown. In contrast, our T1KO mice quantitatively exhibited a 50% reduction in CS (Fig. 1) and a decrease in CS in PNNs, but the absence of a decrease in AGR (+) area or PV (+) cells (Figs. 2 and 3). Thus, T1KO may be a better model for abnormal PNNs than ChABC-treated mice. Recently, in the KO of a link protein Bral2, a component of PNNs, abnormal acoustic startle tests are reported , may support our results (Fig. 4d).
Taken together, we conclude that T1 is the most important factor that controls CS-dependent brain functions. In addition, T1KO may be the most suitable genetic model for studying the functions of PNNs. To further examine the mechanism of CS synthesis and its biological significance, we are planning to analyze mice that are null for CSGalNAcT2 (T2), which is the other isoform of T1, and/or conditional T1/T2 KO mice. For example, most learning and memory tests in T1KO did not show a significant difference from WT, probably because they are dependent upon hippocampal activity . In T1KO, hippocampal PNNs were significantly but only slightly reduced compared to PNNs in other brain regions (Figs. 2 and 3). T1/T2 double knockouts will probably be embryonic lethal due to induction of skeletal defects (M.I., in preparation). However, using T2KO, T1KO/T2 heterozygotes, and T1 heterozygotes/T2KO, larger effects of decreased CS in PNNs are expected.
T1KO, T1 heterozygotes, and WT littermates on the C57BL/6 background were maintained [7, 9]. Four T1KO or four WT mice were housed per cage, and the mice were group-housed in a room with a 12-h light/dark cycle.
Quantitative biochemical analysis of CS
Purification and quantification of CS in various areas of the brain were performed as described previously . In brief, the mice were sacrificed by decapitation. Various regions of the adult mouse brain were dissected, homogenized, and treated with protease (0.01 mg actinase E, 10 mM CaCl2, 50 mM Tris-HCl, pH 8.0) for 2 days at 55 °C. After trichloroacetic acid treatment, the supernatant was gel filtered (Sephadex G-25, 8.3 ml (PD-10 column), and the flow-through fractions were collected. To analyze the disaccharide composition, fractions were treated with ChABC (5 mIU ChABC in 60 mM CH3COONa, 50 mM Tris-HCl, pH 8.0) for 12 h at 37 °C. The disaccharides were labeled with 2-aminobenzamide (350 mM 2-aminobenzamide, 1 M NaCNBH3 in DMSO/acetic acid (7:3)) for 2 h at 65 °C and analyzed quantitatively using high-performance liquid chromatography (column: YMC pack PA, 4.6 × 250 mm, elution: 16–530 mM NaH2PO4, flow rate: 1 ml/min; detection: 330 nm excitation and 420 nm emission). The disaccharide analysis data are shown in Table 1.
Histochemistry and immunohistochemistry
Histological procedures were carried out according to previously described methods . Antibodies against AGR (rabbit polyclonal) and PV (mouse monoclonal) were purchased from Millipore, and from Swant Inc. (Switzerland), respectively. For tissue preparation, under deep anesthesia with isoflurane, the brain was fixed by cardiac perfusion with PBS followed by ice-cold 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. The brain was dissected out, immersed in the same fixative overnight, and transferred to 20% sucrose in 20 mM PBS, pH 7.4 until it sank. Each brain was frozen in crushed dry ice, and 30 μm-thick consecutive coronal sections were cut on a cryostat and stored at −20 °C until histological staining.
Sections were initially rinsed in 20 mM PBS and incubated in 0.1% Triton X-100 for 15 min at room temperature. After rinsing in 20 mM PBS, the sections were incubated overnight at 4 °C with a mixture of biotinylated-WFA (Sigma-Aldrich) and primary antibodies diluted with 20 mM PBS containing 0.5% skim milk. After rinsing in 20 mM PBS for 15 min, sections were incubated with a mixture of streptavidin-Alexa Fluor® 647 conjugate (1:100; Molecular Probes, Inc., Eugene, OR) and secondary antibodies conjugated to Alexa Fluor®488 or Alexa Fluor®594 (1:100; Jackson ImmunoResearch Laboratories, West Grove, PA) for 60 min at 37 °C. After rinsing with 20 mM PBS for 15 min, sections were mounted on MAS®-coated glass slides (Matsunami Glass, Osaka, Japan) and coverslipped with ProLong Gold (Molecular Probes). Sections were observed and digital images were recorded on a confocal laser scanning microscope (FV-1200, Olympus, Tokyo, Japan). TIF files were processed with Photoshop software (Adobe, San Jose, CA). Both brightness and background were adequately adjusted.
For DAB staining, free-floating sections were initially rinsed in 20 mM PBS and incubated in a mixture of 3% hydrogen peroxide and 0.1% Triton X-100 for 15 min at room temperature. 2H6 (Seikagaku Corporation, Tokyo, Japan) was diluted in 20 mM PBS containing 0.5% skim milk. For 2H6 staining, following rinsing in 20 mM PBS for 15 min, sections were incubated with biotinylated anti-mouse IgM secondary antibody (1:200; Vector Laboratories, Burlingame, CA) for 30 min at 37 °C. After rinsing with 20 mM PBS for 15 min, sections were incubated in avidin-biotin peroxidase complex (Vectastain ABC kit, Vector Laboratories) for 30 min (2H6) or 60 min (WFA) at 37 °C. After rinsing with 20 mM PBS, immunoreaction was visualized in 50 mM Tris-HCl buffer (pH 7.4) containing 0.01% DAB and 0.01% hydrogen peroxide at 37 °C for 5–10 min. Sections were mounted on MAS-coated glass slides (Matsunami Glass), air-dried on a hot plate at 40 °C, and coverslipped with Entellan Neu (Merck, Darmstadt, Germany) after dehydration through ethanol and xylene.
Dilution ratios: were as follows: WFA, 1:50; 2H6, 1:100; anti-PV, 1:500; and anti-AGR, 1:500.
We initially counted the number of PV (+) cells. However, that method was not suitable for exact quantification of PV (+) cells because the staining of PV (+) cells varied widely. Thus, we switched to the following quantification methods. The fluorescent intensity of the immunohistochemical and WFA histochemical images was statistically calculated using Image J. Double- (AGR and WFA) or triple (AGR, WFA, and PV)-labeled confocal images were used for the intensity analysis. AGR+ pixels were extracted by masking with an intensity threshold (>50). The average intensity of the AGR+ pixels was calculated for each AGR, WFA, or PV image. The average intensity data were grouped by genotype and brain area. Two-way factorial ANOVA with Bonferroni post-hoc tests were performed for statistical analysis.
Animal behavioral tests
A battery of mouse behavioral tests was done as described previously [25, 26]. All behavioral tests were carried out with male mice. A general health check and neurological screen were conducted as previously described . The order in which mice were subjected to tests was counterbalanced. Raw data were disclosed in the Mouse Phenotype Database (http://www.mouse-phenotype.org/). T1KO and WT mice (n = 20 each) at 8 weeks of age were tested. The tests that showed statistically significant differences in T1KO vs. WT were:
(I) Open field test: This test was performed to evaluate locomotor activity and emotional response . The apparatus was a transparent square cage (42 × 42 × 30 cm; Accuscan Instruments, Columbus, OH). The center of the floor was illuminated at 100 lx. Each mouse was placed in the open field apparatus and recorded for 120 min. Total distance traveled (cm), vertical activity (rearing measured by counting the number of photobeam interruptions), time spent in the center area (20 × 20 cm), and the beam-break counts for stereotyped behaviors were measured.
(II) Social interaction test in a novel environment : Two mice of the same genotype that were previously housed in different cages were placed in a box together (40 × 40 × 30 cm; O’Hara & Co., Tokyo, Japan) and allowed to explore freely for 10 min. Mouse behavior was analyzed automatically using ImageSI software. The total duration of contacts (s), number of contacts, total duration of active contacts (s), mean duration per contact (s), and total distance traveled (cm) were measured. Active contact was defined as follows: images were captured at three frames per second, and distance traveled between two successive frames was calculated for each mouse. If the two mice contacted each other and the distance traveled by either mouse was 5 cm or more, the behavior was considered an “active contact.”
(III) Three-chambered social approach test: The test for sociability and social novelty preference was conducted as previously described [28, 29]. For habituation to the test environment, stranger mice were placed in a small cylindrical cage with vertical bars that was put in a corner of the chamber prior to the test. Test mice were placed in the middle chamber and allowed to explore and habituate to the chambers for 10 min just before the first session. In the first session, an unfamiliar mouse (stranger 1) was put in the cage and placed in a corner of the left or right side chamber. The selection of the left or right side chamber was counterbalanced across test mice. An empty cage was placed in the corner of the other chamber. Then the test mouse was placed in the middle chamber and allowed to explore the three chambers for 10 min. In the second session, another unfamiliar mouse (stranger 2) was placed in the cage that had been empty during the first session. Then the test mouse was placed in the middle chamber and allowed to explore for 10 min. Total distance travelled, average locomotion speed, and the amount of time spent around the cages were measured. Data acquisition and analysis were performed automatically using ImageSI.
(IV) Startle response/prepulse inhibition test: A startle reflex measurement system (O’Hara & Co.) was used to measure startle response to a loud noise and prepulse inhibition of the startle response. A test session began by placing a mouse in a plastic cylinder where it was left undisturbed for 10 min. White noise (40 ms) was used as the startle stimulus for all trial types. The startle response was recorded for 400 ms starting with the onset of the startle stimulus. The background noise level was 70 dB. The peak startle amplitude was the dependent variable. A test session consisted of six trial types (e.g., two types of startle-stimulus-only trials, and four types for prepulse inhibition trials). The intensity of the startle stimulus was either 110 or 120 dB. The prepulse sound was presented 100 ms before the onset of the startle stimulus, and its intensity was 74 or 78 dB (20 ms). Four combinations of prepulse and startle stimuli were used (74–110, 78–110, 74–120, and 78–120 dB). Six blocks of the six trial types were presented in a pseudorandom order such that each trial type was presented once within a block. The average inter-trial interval was 15 s (range: 10–20 s). Behavioral tests that were not significantly different between genotypes are listed in Table 2. Behavioral data were obtained automatically by applications (ImageLD, EP, SI, CSI, PS, FZ, TS, HCSI, and BM) based on the public domain Image J program (http://rsb.info.nih.gov/ij/) and modified for each test by the authors. Statistical analysis was conducted using StatView (SAS Institute, Cary, NC). Data were analyzed with the Student’s t-test, paired t-test, one-way ANOVA, or two-way repeated measures ANOVA. Values in graphs are presented as the mean ± SEM.
We thank Y. Wada (Igarashi lab) for her technical assistance and the Animal Resource Department of Niigata University for production and maintenance of T1KO.
This work was supported in part by TOGO-NO and HOKATSU-NO (Comprehensive Brain Science Network) on Innovative Areas (to M.I.), Project Promoting Grants from Niigata University, Uehara Memorial Science Promoting Foundation (to M.I.), RIKEN BRC Grants for gene targeting in mice (to M.I.), KAKENHI (#17023019, #22240040, and #24111515 to M.I) from MEXT of Japan and JSPS, Platform of Advanced Animal Model Support from MEXT of Japan (to M.I., K.T., and T.M), and Sasakawa Scientific Research Grant from The Japan Science Society (to N.Y.).
Availability of data and materials
The datasets supporting the conclusion of this study are included in this article.
MI, NY, and KT designed the experiments. SM and HK quantitatively analyzed the CS biochemical data. NY and KT performed the histochemical analysis; NY and AT quantified these results. YW and AK produced and maintained T1KO. TK and TM designed the behavioral test battery, and TK performed the tests. MI, NY, AT, and AK wrote the paper. All authors read and approved the final manuscript.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Sugiyama S, Di Nardo AA, Aizawa S, Matsuo I, Volovitch M, Prochiantz A, Hensch TK. Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity. Cell. 2008;134:508–20.View ArticlePubMedGoogle Scholar
- Tsien RY. Very long-term memories may be stored in the pattern of holes in the perineuronal net. Proc Natl Acad Sci U S A. 2013;110:12456–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Fawcett JW. The extracellular matrix in plasticity and regeneration after CNS injury and neurodegenerative disease. Prog Brain Res. 2015;218:213–26.View ArticlePubMedGoogle Scholar
- Hunter AM, Leuchter AF, Power RA, Muthén B, McGrath PJ, Lewis CM, Cook IA, Garriock HA, McGuffin P, Uher R, Hamilton SP. A genome-wide association study of a sustained pattern of antidepressant response. J Psychiatr Res 2013; 47: 1157-1165. 5.Google Scholar
- Bitanihirwe BK, Woo TU. Perineuronal nets and schizophrenia: the importance of neuronal coatings. Neurosci Biobehav Rev. 2014;45:85–99.View ArticlePubMedPubMed CentralGoogle Scholar
- Do KQ, Cuenod M, Hensch TK. Targeting oxidative stress and aberrant critical period plasticity in the developmental trajectory to schizophrenia. Schizophr Bull. 2015;41:835–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Watanabe Y, Takeuchi K, Higa Onaga S, Sato M, Tsujita M, Abe M, Natsume R, Li M, Furuichi T, Saeki M, Izumikawa T, Hasegawa A, Yokoyama M, Ikegawa S, Sakimura K, Amizuka N, Kitagawa H, Igarashi M. Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development. Biochem J. 2010;432:47–55.View ArticlePubMedPubMed CentralGoogle Scholar
- Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H. Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism. J Biol Chem. 2011;286:5803–12.View ArticlePubMedGoogle Scholar
- Takeuchi K, Yoshioka N, Higa Onaga S, Watanabe Y, Miyata S, Wada Y, Kudo C, Okada M, Ohko K, Oda K, Sato T, Yokoyama M, Matsushita N, Nakamura M, Okano H, Sakimura K, Kawano H, Kitagawa H, Igarashi M. Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury. Nat Commun. 2013;4:2740.View ArticlePubMedPubMed CentralGoogle Scholar
- Mikami T, Kitagawa H. Biosynthesis and function of chondroitin sulfate. Biochim Biophys Acta. 1830;2013:4719–33.Google Scholar
- Dick G, Tan CL, Alves JN, Ehlert EM, Miller GM, Hsieh-Wilson LC, Sugahara K, Oosterhof A, van Kuppevelt TH, Verhaagen J, Fawcett JW, Kwok JC. Semaphorin 3A binds to the perineuronal nets via chondroitin sulfate type E motifs in rodent brains. J Biol Chem. 2013;288:27384–95.View ArticlePubMedPubMed CentralGoogle Scholar
- Ito H, Nagano M, Suzuki H, Murakoshi T. Chronic stress enhances synaptic plasticity due to disinhibition in the anterior cingulate cortex and induces hyper-locomotion in mice. Neuropharmacology. 2010;58:746–57.View ArticlePubMedGoogle Scholar
- Hu H, Gan J, Jonas P. Interneurons. Fast-spiking, parvalbumin+ GABAergic interneurons: from cellular design to microcircuit function. Science. 2014;345:1255263.View ArticlePubMedGoogle Scholar
- Paine TA, Swedlow N, Swetschinski L. Decreasing GABA function within the medial prefrontal cortex or basolateral amygdala decreases sociability. Behav Brain Res. 2017;317:542–52.View ArticlePubMedGoogle Scholar
- Takao K, Miyakawa T. Intrauterine environment-genome interaction and children's development (4): Brain-behavior phenotypying of genetically-engineered mice using a comprehensive behavioral test battery on research of neuropsychiatric disorders. J Toxicol Sci. 2009;34:SP293–305.View ArticlePubMedGoogle Scholar
- Pantazopoulos H, Berretta S. In Sickness and in Health: Perineuronal Nets and Synaptic Plasticity in Psychiatric Disorders. Neural Plast. 2016;2016:9847696.View ArticlePubMedGoogle Scholar
- Chang YC, Cole TB, Costa LG. Behavioral Phenotyping for Autism Spectrum Disorders in Mice. Curr Protoc Toxicol. 2017;72:11.22.1–11.22.21.View ArticleGoogle Scholar
- Gogolla N, Caroni P, Lüthi A, Herry C. Perineuronal nets protect fear memories from erasure. Science. 2009;325:1258–61.View ArticlePubMedGoogle Scholar
- Kim JH, Richardson R. A developmental dissociation of context and GABA effects on extinguished fear in rats. Behav Neurosci. 2007;121:131–9.View ArticlePubMedGoogle Scholar
- Hou X, Yoshioka N, Tsukano H, Sakai A, Miyata S, Watanabe Y, Yanagawa Y, Sakimura K, Takeuchi K, Kitagawa H, Hensch TK, Shibuki K, Igarashi M, Sugiyama S. Chondroitin sulfate is required for onset and offset of critical period plasticity in visual cortex. SciRep. 2017; in pressGoogle Scholar
- Simonetti T, Lee H, Bourke M, Leamey CA, Sawatari A. Enrichment from birth accelerates the functional and cellular development of a motor control area in the mouse. PLoS One. 2009;4:e6780.View ArticlePubMedPubMed CentralGoogle Scholar
- Avram S, Shaposhnikov S, Buiu C, Mernea M. Chondroitin sulfate proteoglycans: structure-function relationship with implication in neural development and brain disorders. Biomed Res Int. 2014;2014:642798.View ArticlePubMedPubMed CentralGoogle Scholar
- Popelář J, Díaz Gómez M, Lindovský J, Rybalko N, Burianová J, Oohashi T, Syka J. The absence of brain-specific link protein Bral2 in perineuronal nets hampers auditory temporal resolution and neural adaptation in mice. Physiol Res. 2017; in press (Jul 18. [Epub ahead of print]).Google Scholar
- Yoshioka N, Asou H, Hisanaga S, Kawano H. The astrocytic lineage marker calmodulin-regulated spectrin-associated protein 1 (Camsap1): phenotypic heterogeneity of newly born Camsap1-expressing cells in injured mouse brain. J Comp Neurol. 2012;520:1301–17.View ArticlePubMedGoogle Scholar
- Takao K, Yamasaki N, Miyakawa T. Impact of brain-behavior phenotypying of genetically-engineered mice on research of neuropsychiatric disorders. Neurosci Res. 2007;58:124–32.View ArticlePubMedGoogle Scholar
- Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, Takao K, Fukaya M, Miyakawa T, Watanabe M, Sakimura K, Manabe T, Igarashi M. Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity. J Biol Chem. 2013;288:34906–19.View ArticlePubMedPubMed CentralGoogle Scholar
- Shoji H, Takao K, Hattori S, Miyakawa T. Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age. Mol Brain. 2016;9:11.View ArticlePubMedPubMed CentralGoogle Scholar
- Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol. 2003;463:3–33.View ArticlePubMedGoogle Scholar
- Takao K, Tanda K, Nakamura K, Kasahara J, Nakao K, Katsuki M, Nakanishi K, Yamasaki N, Toyama K, Adachi M, Umeda M, Araki T, Fukunaga K, Kondo H, Sakagami H, Miyakawa T. () Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice. PLoS One. 2010;5:e9460.View ArticlePubMedPubMed CentralGoogle Scholar