- Open Access
Vps35 haploinsufficiency results in degenerative-like deficit in mouse retinal ganglion neurons and impairment of optic nerve injury-induced gliosis
© Liu et al.; licensee BioMed Central Ltd. 2014
- Received: 14 November 2013
- Accepted: 8 February 2014
- Published: 11 February 2014
VPS35 (vacuolar protein sorting 35) is a major component of retromer that selectively promotes endosome-to-Golgi retrieval of transmembrane proteins. Dysfunction of retromer is a risk factor for the pathogenesis of Parkinson’s disease (PD) and Alzheimer’s disease (AD), both neuro-degeneration disorders. However, VPS35/retromer’s function in retina or the contribution of Vps35-deficiency to retinal neuro-degenerative disorders has not been investigated. Here we provide evidence for a role of VPS35 in mouse retinal ganglion cell (RGC) survival and regeneration. VPS35 is selectively expressed in developing mouse RGCs. RGCs from young adult Vps35 heterozygotes (Vps35+/m) show degenerative-like features, such as dystrophic dendrites, reduced axon fibers, and increased TUNEL labeled RGCs. Additionally, gliosis in the optic nerve is transiently elevated in neonatal, but reduced in aged Vps35+/m mice. Optic nerve injury-induced gliosis is also attenuated in Vps35+/m mice. These results suggest that Vps35 is necessary for mouse RGC survival and regeneration, and Vps35-deficiency may contribute to the pathogenesis of retinal ganglion neuro-degeneration, a critical pathology leading to the blindness of many retinal degenerative disorders.
- Retinal ganglion cell
- Optic nerve injury
Retromer is essential for selective retrieval of transmembrane proteins from endosomes to trans-Golgi network [1–3]. Retromer contains two sub-protein complexes: the cargo-selective complex and membrane deformation complex [2, 4]. VPS35 is the key component of the cargo-selective complex, a trimer of VPS proteins VPS35, VPS29, and VPS26. Dysfunction of VPS35/retromer is a risk factor for neuro-degenerative disorders, including Parkison’s disease (PD) and Alzheimer’s disease (AD) [5–9]. Mutations in Vps35 gene is identified in patients of late-onset PD [5, 6]. The retromer complex (e.g., Vps35 and Vps26) is decreased in the postmortem hippocampus of AD patients . In Vps35 or Vps26 deficient animals, the major culprit of AD, β-amyloid (Aβ), is increased in the hippocampus [7, 9]. In addition, Vps35 haploinsufficiency in Tg2576 mouse model of AD enhances Aβ-associated neuropathology . Furthermore, using in utero electroporation, suppression of Vps35 expression in embryonic hippocampal neurons results in “degenerative-like” phenotypes . These observations thus suggest a critical role for VPS35/retromer in preventing neuro-degeneration.
Retinal ganglion cells (RGCs), important neurons in the retina, receive visual information from photoreceptors via two intermediate neurons (bipolar and amacrine cells) and deliver the signal via their axons. RGC’s axons form nerve fibers, extend into the optic nerve through optic disc, further form the optic chiasm and optic tract, and finally transmit visual information from the eye to the brain. Dysfunction of RGCs or degeneration of RGCs is a major pathology detected in several retinal disorders, including glaucoma [11, 12] and age-related macular degeneration(AMD) [12, 13], which is a main reason leading to irreversible blindness.
Although dysfunction of Vps35 is implicated in the pathogenesis of AD and PD, its function in the retina is largely unknown. We thus asked: where in the retina Vps35 is expressed? Does loss of Vps35 contribute to the retinal neuron degeneration? Here, we started to shed light on these questions by demonstrating Vps35 expression selectively in mouse RGCs. RGCs in Vps35 heterozygote mice (named vps35+/m) exhibit degenerative-like morphology, such as disturbed dendritic processes, reduced axonal fibers, and increased RGC apoptosis. In addition, Vps35+/m mice show impairment in optic nerve injury-induced gliosis, implicating Vps35 in regulating optic nerve regeneration.
VPS35 expression in developing mouse RGCs, including melanopsin positive ipRGCs
We then examined VPS35’s expression by Western blot analysis. Consistently, An age-dependent reduction of ß-galactosidase protein was observed in vps35+/m retinas (Figures 1C-D). However, using anti-VPS35 antibody, the VPS35 protein levels were comparable among different aged vps35+/+ retinas (Figures 1C and E). ~50% reductions of Vps35 proteins were detected in young (P1 to P60), but not aged (P90 to P180), Vps35+/m retinas (Figures 1C and E), implicating a compensatory effect in aged mutant mice. The differential results by anti-ß-galactosidase and anti-VPS35 antibodies may result from an age-dependent down-regulation of vps35’s transcriptional expression (viewed by its LacZ reporter) or ß-galactosidase protein stability, but not VPS35 protein. To distinguish these possibilities, we examined vps35’s transcripts from different aged Vps35+/+ and +/m retinas by real time PCR (RT-PCR) analysis. Vps35’s mRNAs appeared to be unchanged among various aged Vps35+/+ retinas, but decreased in Vps35+/m controls (Additional file 1: Figure S1A). This result demonstrates the specificity of the analysis and excludes the possibility for age-dependent inhibition of vps35’s transcription. In line with this view, little change was observed for the LacZ transcripts detected in various aged Vps35+/m retinas, but not in Vps35+/+ controls (Additional file 1: Figure S1B). Taken together, these results suggest that Vps35 appears to be stably expressed in mouse retina throughout development, and the reduced LacZ staining/protein level in aged mutant retina may be due to an-age dependent instability of the LacZ protein. The stable VPS35 expression in different aged retina GCL was further supported by immunostaining analysis (Additional file 1: Figure S1C). Note that vps35+/m retina (particularly at neonatal age) showed ~50% reduction of VPS35 protein (Figures 1C, E), demonstrating the antibody specificity. VPS26, another component of retromer, was also decreased in vps35+/m retina (Figure 1C). These results thus suggested that the retromer complex is stably expressed in developing mouse retina, which is mainly distributed in the retinal GCL and lost in vps35+/m mice.
Note that a few β-gal positive cells were also distributed in IPL (inner plexiform layer), in addition to GCL layer (Figure 1B). Such a distribution pattern is similar to that of M1 type of ipRGCs (intrinsically photo-sensitive retinal ganglion cells) that express melanopsin [15, 16]. We thus examined if Vps35 is co-distributed with melanopsin in ipRGCs. Indeed, fractions, but not all, of β-Gal positive cells were also melanopsin positive (Figure 2C). Taken together, these results suggested that VPS35 is expressed in developing mouse RGCs, including ipRGCs.
Degenerative-like deficit in vps35+/m RGCs
Next we asked whether VPS35 plays a role in RGC development and survival. RGC starts to develop around E10.5 and finished its development in neonatal age . We carried out immunostaining analysis of P1 flat-mounted eye disc using antibodies against neurofilament or Tuj1, which recognize RGCs’ soma, axons, and dendrites (by Tuj1). A normal distributed RGC neuronal somas and axonal fibers in Vps35+/m retina were observed (Additional file 2: Figure S2), suggesting little, if there is any, role of VPS35 in RGC development.
Reduced optic nerve injury-induced gliosis in neonatal Vps35+/m mice
We then asked if Vps35 deficiency affects optic nerve injury-induce gliosis, a critical process associated with optic nerve regeneration . Optic nerves were crushed at the site close to the optic disc (Figure 6B). 3-days after injury, mice were sacrificed and optic nerves were examined by immunostaining analysis. Upon nerve injury, gliosis, viewed by anti-GFAP, MBP, and IBA1 antibodies, was increased in vps35+/+ optic nerves (Figures 6C-D). MBP-labeled oligodendritecytes appeared to be shifted to the site of optic disc (Figures 6C-D). In contrast, the nerve injury induced gliosis, marked by GFAP and IBA1 antibodies, was attenuated in vps35+/m mice (Figures 6C-D), suggesting an impairment of optic nerve injury-induced gliosis.
VPS35 is a key element of retromer that is a heteropentameric complex with a transmembrane protein sorting function from the endosome to the trans- Golgi network [1–3]. Dysfunction of VPS35/retromer is a risk factor for a number of neurodegenerative disorders, including PD and AD [5–9, 20]. Given VPS35’s crucial function in suppressing neuro-degeneration, we asked if it plays a role in retinal neuron survival. Here, we presented several lines of evidences for a critical role of VPS35 in mouse RGC survival. First, VPS35 is selectively expressed in developing mouse RGCs. Second, vps35+/m retina show degenerative-like RGCs, with disturbed RGC dendrites, reduced RGC axon fibers, and increased apoptosis. Third, vps35+/m optic nerves exhibit transient elevation of gliosis in neonatal age, but reduced in aged mice. Finally, optic nerve injury-induced gliosis is attenuated in vps35+/m mice.
Several characteristics of Vps35’s expression in mouse retina are noted. It is selectively expressed in mouse RGCs. This is based on in large the distribution of the LacZ reporter in the Vps35 gene. This view is also confirmed by immunostaining analysis using anti-VPS35 antibody. The LacZ reporter expression in RGCs is reduced during aging. However, no obvious reduction in Vps35 protein levels was detected by Western blot analysis of the retina homogenates. These results implicate that Vps35’s protein may be very stable, but its transcript could be reduced during aging. In addition, VPS35 is expressed in subset of RGCs, including ipRGCs. However, the function of VPS35 in ipRGCs remains to be further investigated.
The degenerative-like morphology of vps35+/- RGCs may be largely caused by the reduced VPS35 expression in RGCs. This view is in line with the observation of the selective expression of VPS35 in developing RGCs. However, we cannot exclude the possibility of cell non-autonomous mechanisms, as Vps35 is also weakly expressed in glial cells, such as astrocytes and microglia, and vps35+/m mice show altered gliosis (Figure 6) and increased inflammation . The latter may contribute further RGC degeneration.
Gliosis, a nonspecific reactive change of glial cells in response to damage to the neurons, involves the proliferation of different types of glial cells, including astrocytes, microglia, and oligodendrocytes. Gliosis has both beneficial and detrimental effects. Its beneficial effects include neuroprotection, maintenance of the extracellular environment, seclusion of the injury site, and nerve regeneration [21, 22]. Its detrimental effects are restriction of axon regeneration when there is glial scar formation. Vps35+/m mice showed an-age dependent gliosis, detected in neonatal, but not aged, mice. Such a transient gliosis may be the consequences of the RGC degeneration. Interestingly, optic nerve injury-induced gliosis was attenuated in Vps35+/m mice, implicating that VPS35 may play a role in nerve regeneration.
The retinal degeneration is a major pathology associated with glaucoma [11, 12] and age-related macular degeneration (AMD) [12, 13]. It is of interest to note that glaucoma or AMD is often associated with other neurodegenerative disorders, including AD and PD . Mutations in Vps35 gene are identified in late onset PD patients [5, 6]. Dysfunction of retromer is also believed to be a risk factor for AD [7, 9]. However, it remains unknown if there is any mutation in vps35 gene or other retromer component in patients with glaucoma or AMD. Given the fact we demonstrate here that VPS35 regulates RGC survival, plus the association of retinal RGC degeneration with neuro-degeneration, it is conceivable that dysfunction of VPS35 or other retromer component may contribute to the pathogenesis of glaucoma or AMD patients. Clearly, this requires further analysis of the putative causative genes in glaucoma or AMD patients.
Animals and reagents
Vps35 mutant mice have been described previously [9, 10, 14], which were backcrossed with C57BL/6 mice for more than 10 generations. Mice were maintained on a standard rodent diet. Vps35 mutations were confirmed by genotyping using PCR and Western blot analysis. All experimental procedures were approved by the Animal Subjects Committee at the Georgia Regents University according to US National Institutes of Health guidelines.
Rabbit polyclonal anti-VPS35 antibody was generated using the antigen of GST-VPS35D1 fusion protein as described previously [9, 10, 14]. Rabbit polyclonal antibodies, including anti- ß-galactosidase (Cappel), anti-VPS26 (Abcam),anti-calretinin (Swant), and anti-melanopsin (Advanced Targeting Systems) antibodies, were purchased. Mouse monoclonal antibodies, including anti-neuronal class III ß-Tubulin (Tuj1,Convance), anti-neurofilament (DSHB), anti-calbindin D28k (Swant), anti-glial fibrillary acidic protein (GFAP, Chemicon), anti-rhodopsin (Abcam) and anti-myelin basic protein (MBP, Chemicon) antibodies, were also purchased. In addition, the chicken polyclonal anti- ß-galactosidase antibody (Abcam) and goat polyclonal anti-IBA1 antibody (Abcam) were used. Secondary antibodies were purchased from Jackson Immuno Research Laboratories, Inc. Other chemicals and reagents used in this study were of analytical grade.
β-Gal detection, immunofluorescence staining, and confocal imaging analysis
Vps35+/+ or +/m mice were sacrificed and the eyes were excised. For β-Gal detection, the eyes were immediately embedded in OCT medium (Sakura Finetek, Torrance, CA), and frozen at -80°C for 2h. 20 μm-thick sections were cut on a cryostat and mounted onto Super-Frost Plus slides. For immunofluorescence staining, after eyes excised, the corneas were removed, eye cups were fixed in 4% paraformaldehyde in 0.1 M PBS at 4°C for 24 h. Lenses were then removed, the retina to be flat-mounted were dissected from eye cups and processed in 1.5ml microfuge tubes. Eye cups for cross sectioning were cryo-protected with 30% sucrose in PBS at 4°C for 24 h, embedded in OCT medium (Sakura Finetek, Torrance, CA), frozen, sectioned at 12 μm-thickness, and mounted onto Super-Frost Plus slides.
β-Gal activity was detected as described previously [9, 10, 14, 23, 24]. In brief, frozen eye sections derived from Vps35+/+ and Vps35+/m mice were fixed with 0.5% glutaraldehyde and incubated with X-gal solution (2 mM MgCl2, 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, and 0.1% X-gal) in the dark at 37°C for 12 h. The slides were washed, mounted in permount (Thermo Fisher Scientific), and imaged using deconvolution digital microscope (Axioplan 2; Carl Zeiss) with high sensitivity camera (AxioCam; Carl Zeiss) and equipped with a plan-neofluar 5× and plan-neofluar 10×/0.30 NA objective lens.
For immunofluorescence staining analysis, flat mounts or cross-sections were post-fixed with 4% PFA at room temperature for 15 min, permeabilized with 0.3% Triton X-100 at room temperature for 20 min, and blocked with 10% horse serum and 5% BSA at room temperature for 2 h. Flat mounts or sections were incubated for 24 h at 4°C in primary antibodies. Antibodies used were rabbit polyclonal anti-VPS35 (1:1,000), mouse monoclonal anti-Tuj1 (Convance, 1:1,000), mouse monoclonal anti-neurofilament (DSHB, 1:1,000), rabbit polyclonal anti-calretinin (Swant, 1:500), rabbit polyclonal anti-melanopsin (Advanced Targeting Systems, 1:5,000), mouse monoclonal anti-calbindin D28k (Swant, 1:2,000), mouse monoclonal anti-GFAP (Chemicon, 1:1,000), mouse monoclonal anti-Rhodopsin (Abcam, 1:500), mouse monoclonal anti-MBP (Chemicon, 1:500), goat polyclonal anti-IBA1 antibody (Abcam, 1:500) and chicken polyclonal anti-ß-galactosidase (Abcam, 1:1,000). Flat mounts or sections were incubated with appropriate fluorescent secondary antibodies at room temperature for 2 h, then incubated with Topro3 (1:5,000) at room temperature for 10 min. Slides were mounted using VECTASHIELD mounting medium. Confocal images were obtained using Nikon C1 confocal system. For fluorescent quantification, morphometric measurements of images were performed using Image- pro plus software (Media Cybernetics).
Tissue lysis and western blotting
Retina and optic nerve tissues were lysed in lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% Triton X-100, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM EDTA, 5 mM sodium fluoride, 2 mM sodium orthovanadate and protease inhibitors) 15 minutes at 4°C and centrifuged at 14,000 × g, protein concentration was determined by BCA protein assay kit (Thermo). Resulting supernatants separated by SDS-PAGE in 8%–12% gradient gels. Electroblotting was at 4°C for 2 hours (100V, 400 mA). Membranes were blocked in 10% silk milk at room temperature for 1h. Primary antibody incubation was overnight at 4°C. After 3× washes with TBST (Tris-Buffered Saline and Tween 20), membranes were incubated in secondary antibodies for 1 h at room temperature. Primary antibodies included rabbit polyclonal anti-VPS35 (1:10,000), rabbit polyclonal anti-VPS26 (Abcam, 1:2,000), rabbit polyclonal anti-ß-galactosidase (Cappel, 1:2,000), mouse monoclonal anti-Tuj1 (Convance, 1:4,000), mouse monoclonal anti-GFAP (Chemicon, 1:1,000) and mouse monoclonal anti-MBP (Chemicon, 1:1,000). Secondary antibodies (all 1:8,000 dilution; all Thermo) were anti-mouse and anti-rabbit HRP. Band intensities were quantified densitometrically and normalized to ß-actin. For semi-quantitative analysis, protein bands detected by ECL were scanned into Adobe Photoshop CS5 and analyzed using ImageJ software (National Institutes of Health). Care was taken during exposure of the ECL film to ensure that intensity readouts were in a linear range of standard curve blot detected by the same antibody.
Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL)
Sections were processed for TUNEL as described above. TUNEL assay was performed using the In Situ Cell Death Detection Kit (Roche).
Optic nerve injury
Optic nerve injury was performed in Vps35+/+ and Vps35 +/m mice at P30 as described previously . Only the left optic nerve was injured, the right optic nerve served as negative control. Mice were anesthetized by intraperitoneal injection of Ketamine and Xylazine (100 mg/kg and 20 mg/kg body weight). The incision was made in the conjunctiva at the superior pole of the eye, then a blunt dissection of the conjunctiva was made with forceps towards the back of the eye to expose the retrobulbar optic nerve. The optic nerve was crushed with forcep for 10 seconds at 1 mm behind the globe. After the surgery, tobramyclin ointment was applied to avoid infection and the animal recovered on a warming pad. After 3 days, mice were sacrificed, the eyes were excised, then removed the optic nerves from the eye-balls. The optic nerves were fixed in 4% paraformaldehyde in 0.1 M PBS at 4°C for 24 h and cryo-protected with 30% sucrose in PBS at 4°C for 24 h, embedded in OCT medium(Sakura Finetek, Torrance, CA), frozen, sectioned 12 μm-thickness, and mounted onto Super-Frost Plus slides.
All data were expressed as means ± SEM. Three to five mice per genotype per assay were used. Three to five positions were quantified for immunostaining analyses. The significance level was set at P < 0.05, and the Student’s t test was used.
We thank members of Dr. Xiong laboratories for helpful discussions and suggestions. This work is supported in part by grants from the National Institutes of Health (to W.-C. Xiong) and Veterans Administration (BX000838 to W.-C. Xiong).
- Seaman MN, Marcusson EG, Cereghino JL, Emr SD: Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products. J Cell Biol. 1997, 137 (1): 79-92. 10.1083/jcb.137.1.79.PubMedPubMed CentralView ArticleGoogle Scholar
- Bonifacino JS, Hurley JH: Retromer. Curr Opin Cell Biol. 2008, 20 (4): 427-436. 10.1016/j.ceb.2008.03.009.PubMedPubMed CentralView ArticleGoogle Scholar
- McGough IJ, Cullen PJ: Recent advances in retromer biology. Traffic. 2011, 12 (8): 963-971. 10.1111/j.1600-0854.2011.01201.x.PubMedView ArticleGoogle Scholar
- Seaman MN: Recycle your receptors with retromer. Trends Cell Biol. 2005, 15 (2): 68-75. 10.1016/j.tcb.2004.12.004.PubMedView ArticleGoogle Scholar
- Vilarino-Guell C, Wider C, Ross OA, Dachsel JC, Kachergus JM, Lincoln SJ, Soto-Ortolaza AI, Cobb SA, Wilhoite GJ, Bacon JA, et al: VPS35 mutations in Parkinson disease. Am J Hum Genet. 2011, 89 (1): 162-167. 10.1016/j.ajhg.2011.06.001.PubMedPubMed CentralView ArticleGoogle Scholar
- Zimprich A, Benet-Pages A, Struhal W, Graf E, Eck SH, Offman MN, Haubenberger D, Spielberger S, Schulte EC, Lichtner P, et al: A mutation in VPS35, encoding a subunit of the retromer complex, causes late-onset Parkinson disease. Am J Hum Genet. 2011, 89 (1): 168-175. 10.1016/j.ajhg.2011.06.008.PubMedPubMed CentralView ArticleGoogle Scholar
- Small SA, Kent K, Pierce A, Leung C, Kang MS, Okada H, Honig L, Vonsattel JP, Kim TW: Model-guided microarray implicates the retromer complex in Alzheimer's disease. Ann Neurol. 2005, 58 (6): 909-919. 10.1002/ana.20667.PubMedView ArticleGoogle Scholar
- Lane RF, Raines SM, Steele JW, Ehrlich ME, Lah JA, Small SA, Tanzi RE, Attie AD, Gandy S: Diabetes-associated SorCS1 regulates Alzheimer's amyloid-beta metabolism: evidence for involvement of SorL1 and the retromer complex. J Neurosci. 2010, 30 (39): 13110-13115. 10.1523/JNEUROSCI.3872-10.2010.PubMedPubMed CentralView ArticleGoogle Scholar
- Wen L, Tang FL, Hong Y, Luo SW, Wang CL, He W, Shen C, Jung JU, Xiong F, Lee DH, et al: VPS35 haploinsufficiency increases Alzheimer's disease neuropathology. J Cell Biol. 2011, 195 (5): 765-779. 10.1083/jcb.201105109.PubMedPubMed CentralView ArticleGoogle Scholar
- Wang CL, Tang FL, Peng Y, Shen CY, Mei L, Xiong WC: VPS35 regulates developing mouse hippocampal neuronal morphogenesis by promoting retrograde trafficking of BACE1. Biol Open. 2012, 1 (12): 1248-1257. 10.1242/bio.20122451.PubMedPubMed CentralView ArticleGoogle Scholar
- McKinnon SJ: The cell and molecular biology of glaucoma: common neurodegenerative pathways and relevance to glaucoma. Invest Ophthalmol Vis Sci. 2012, 53 (5): 2485-2487. 10.1167/iovs.12-9483j.PubMedView ArticleGoogle Scholar
- Sivak JM: The aging eye: common degenerative mechanisms between the Alzheimer's brain and retinal disease. Invest Ophthalmol Vis Sci. 2013, 54 (1): 871-880. 10.1167/iovs.12-10827.PubMedView ArticleGoogle Scholar
- Ohno-Matsui K: Parallel findings in age-related macular degeneration and Alzheimer's disease. Prog Retin Eye Res. 2011, 30 (4): 217-238. 10.1016/j.preteyeres.2011.02.004.PubMedView ArticleGoogle Scholar
- Xia WF, Tang FL, Xiong L, Xiong S, Jung JU, Lee DH, Li XS, Feng X, Mei L, Xiong WC: Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via sustained RANKL signaling. J Cell Biol. 2013, 200 (6): 821-837. 10.1083/jcb.201207154.PubMedPubMed CentralView ArticleGoogle Scholar
- Berson DM, Dunn FA, Takao M: Phototransduction by retinal ganglion cells that set the circadian clock. Science. 2002, 295 (5557): 1070-1073. 10.1126/science.1067262.PubMedView ArticleGoogle Scholar
- Sexton T, Buhr E, Van Gelder RN: Melanopsin and mechanisms of non-visual ocular photoreception. J Biol Chem. 2012, 287 (3): 1649-1656. 10.1074/jbc.R111.301226.PubMedPubMed CentralView ArticleGoogle Scholar
- Marquardt T, Gruss P: Generating neuronal diversity in the retina: one for nearly all. Trends Neurosci. 2002, 25 (1): 32-38. 10.1016/S0166-2236(00)02028-2.PubMedView ArticleGoogle Scholar
- Raff MC: Glial cell diversification in the rat optic nerve. Science. 1989, 243 (4897): 1450-1455. 10.1126/science.2648568.PubMedView ArticleGoogle Scholar
- Qu J, Jakobs TC: The time course of gene expression during reactive gliosis in the optic nerve. PLoS One. 2013, 8 (6): e67094-10.1371/journal.pone.0067094.PubMedPubMed CentralView ArticleGoogle Scholar
- Small SA: Retromer sorting: a pathogenic pathway in late-onset Alzheimer disease. Arch Neurol. 2008, 65 (3): 323-328.PubMedPubMed CentralView ArticleGoogle Scholar
- Aronica E, Ravizza T, Zurolo E, Vezzani A: Astrocyte immune responses in epilepsy. Glia. 2012, 60 (8): 1258-1268. 10.1002/glia.22312.PubMedView ArticleGoogle Scholar
- McMillian MK, Thai L, Hong JS, O'Callaghan JP, Pennypacker KR: Brain injury in a dish: a model for reactive gliosis. Trends Neurosci. 1994, 17 (4): 138-142. 10.1016/0166-2236(94)90086-8.PubMedView ArticleGoogle Scholar
- Lee DH, Zhou LJ, Zhou Z, Xie JX, Jung JU, Liu Y, Xi CX, Mei L, Xiong WC: Neogenin inhibits HJV secretion and regulates BMP-induced hepcidin expression and iron homeostasis. Blood. 2010, 115 (15): 3136-3145. 10.1182/blood-2009-11-251199.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhou Z, Xie J, Lee D, Liu Y, Jung J, Zhou L, Xiong S, Mei L, Xiong WC: Neogenin regulation of BMP-induced canonical Smad signaling and endochondral bone formation. Dev Cell. 2010, 19 (1): 90-102. 10.1016/j.devcel.2010.06.016.PubMedPubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.