DIP/WISH deficiency enhances synaptic function and performance in the Barnes maze
© Asrar et al; licensee BioMed Central Ltd. 2011
Received: 15 June 2011
Accepted: 21 October 2011
Published: 21 October 2011
DIP (diaphanous interacting protein)/WISH (WASP interacting SH3 protein) is a protein involved in cytoskeletal signaling which regulates actin cytoskeleton dynamics and/or microtubules mainly through the activity of Rho-related proteins. Although it is well established that: 1) spine-head volumes change dynamically and reflect the strength of the synapse accompanying long-term functional plasticity of glutamatergic synaptic transmission and 2) actin organization is critically involved in spine formation, the involvement of DIP/WISH in these processes is unknown.
We found that DIP/WISH-deficient hippocampal CA1 neurons exhibit enhanced long-term potentiation via modulation of both pre- and post-synaptic events. Consistent with these electrophysiological findings, DIP/WISH-deficient mice, particularly at a relatively young age, found the escape hole more rapidly in the Barnes maze test.
We conclude that DIP/WISH deletion improves performance in the Barnes maze test in mice probably through increased hippocampal long-term potentiation.
DIP (diaphanous interacting protein), also known as WISH (WASP interacting SH3 protein) is a protein involved in cytoskeletal signaling, which regulates actin cytoskeleton and/or microtubule dynamics mainly through negative regulation of Rho . More specifically, we previously reported that DIP/WISH functions as a negative regulator of Rho and a positive regulator of Rho GEF (guanine nucleotide exchange factor) . We also recently showed that mouse embryonic fibroblast (MEF) cells from DIP/WISH-deficient (DIP/WISH-KO) mice have a narrow and long shape with many stress fibers under normal growth conditions . In addition, the DIP/WISH-KO cells exhibited an activation of the Rho-ROCK pathway (particularly with regard to high ROCK activity), a decrease in de novo actin polymerization (especially polymerization requiring arp2/3 activity), and a decrease in cell motility and adhesion as evidenced by an in vitro analysis .
Spine-head volumes are known to change dynamically and reflect synapse strength. Moreover, spine-head volume changes accompany long-term functional plasticity of glutamatergic synaptic transmission, including long-term potentiation (LTP) and long-term depression (LTD) [3, 4]. Actin reorganization represents a primary mechanism necessary to alter spine structures, and actin fibers are most concentrated in dendritic spines. Thus, actin is a critical regulator of spine and dendritic plasticity not only from a biochemical perspective but also from a physical point of view, both of which in turn could modulate LTP and LTD [5, 6]. Involvement of the Rho family protein-mediated pathway in actin polymerization is supported by the fact that abnormalities in actin- and myosin-related proteins cause changes in spine morphology and mental retardation (MR) in mice and humans [7–9]. In particular, ROCK2 deficiency has been shown to cause MR . Additionally, reports that SPIN90, the same protein as DIP/WISH, regulates dendritic spine morphology [11, 12] and that breakpoint cluster region (BCR) and active BCR-related (ABR), both of which activate Rac, positively regulate LTP  support the importance of Rho family proteins for learning and memory via spine formation processes. Given these findings, other pathways altering Rho function could also affect learning and memory behaviors through changes in Rho and ROCK activities.
DIP/WISH is highly expressed throughout the brain including the hippocampus. To determine the involvement of DIP-dependent pathways in hippocampus-mediated events in mouse brain, we examined the expression of several synaptic proteins. In addition, we investigated the physiological function of hippocampal slices from DIP/WISH-KO mice and further conducted a series of behavioral studies with these mice to examine the physiological significance of DIP/WISH in vivo. Furthermore, actin is the main structural component of the stereocilia of cochlear (and vestibular) hair cells. The bundles of cross-linked actin provide the stiffness of the stereocilia which is important for the optimal function of this mechano-receptor. Acoustic signals act to deflect the stereocilia, and it is the relative motion between these stiff structures that opens mechanically gated ion channels to cause hair cell depolarization . One can speculate that even very minor disruptions of the actin bundle integrity might cause a deterioration in threshold sensitivity of hair cells, and therefore auditory system function. Because of this we compared auditory function between the two genotypes using auditory brainstem evoked responses (ABRs).
DIP expression in mouse hippocampus
Altered basal synaptic strength and presynaptic function in DIP/WISH-deficient mice
Enhanced hippocampal long-term potentiation (LTP) in DIP/WISH-deficient mice
Performance in the Barnes circular maze test
Auditory dysfunction in DIP/WISH-KO mice
Our studies of field excitatory postsynaptic potentials and paired-pulse facilitation (Figure 3) suggest abnormalities of both pre- and post-synaptic functions in CA1 hippocampal neurons from DIP/WISH-KO mice. Consistent with these findings, an increased LTP was observed in hippocampal slice-recordings from DIP/WISH-KO mice compared with those from WT mice (Figure 4). The alteration in LTP observed in DIP/WISH-KO mice also raises the possibility of this factor being involved in the regulation of other prominent forms of synaptic plasticity. Towards this end, the opposing phenomenon of LTD  has been suggested to closely associate with LTP in regards to the maintenance of normal synaptic activity. The potentiation-enhancing effect of DIP/WISH seen in our study may imply that this protein could potentially reduce or block this type of depression. Further study of the possible role of DIP/WISH in LTD would be of significant interest. Given that both LTP and LTD can be induced through the actions of NMDA receptors, it would seem a natural extension of the present study to explore modifications at the level of the latter structures in respect to DIP/WISH deletion. This can be accomplished through the utilization of whole-cell recordings examining changes in NMDA-mediated currents in both wild-type and knockout slices, respectively. These electrophysiological changes obtained from the hippocampal neurons of the DIP/WISH-KO mice clearly match the behavioral phenotypes observed, in that DIP/WISH-KO mice found the escape hole in the Barnes maze more rapidly (Figure 5). Behavioral perseveration of the mutants in the probe test after reversal learning in the Barnes maze is also consistent with the results of electrophysiology. Taken together, we propose that DIP/WISH deletion improves the performance in the Barnes maze test through increased hippocampal long-term potentiation in mice.
Enhancement of the Rho-Rock pathway in the DIP/WISH-deficient cells  is most likely related to this phenomenon, and a decrease in de novo actin polymerization (especially polymerization depending on mDia- and WASP-mediated arp2/3 activity) could also be primarily involved. However, the significant differences revealed in the behavioral analysis were observed only at a certain age (up to 3 months of age), suggesting that DIP/WISH functions more critically in the relatively young mouse brain. The result that DIP/WISH-KO mice exhibited impaired auditory function compared with WT mice (Figure 6) without apparent morphological changes (Additional file 1) is in agreement with observation of deafness caused by a mutation of Dia in human  and with the enhancement of synaptic plasticity in DIP/WISH-deficient hippocampal slices.
Improvement of the performance in the Barnes maze test accompanied by increased Rho activity in DIP/WISH-KO mice is also consistent with the fact that both basal synaptic transmission and hippocampal long-term potentiation are impaired in ROCK2-deficient mice . ROCK2-deficient neurons also show deficits in spine properties, synaptic density, and actin cytoskeletal development while these mutant mice are normal in gross brain anatomy  in a similar fashion to DIP/WISH-deficient mice . Taking these results into account, it is likely that ROCK activity is elevated in DIP/WISH-deficient neurons. Recently, different MR-associated genes have been identified that encode modulators (GEF) of the Rho in mice and humans , which supports our result in which Rho activity is elevated. It has been established that loss or mutation of oligophrenin-1, the first identified Rho-linked MR protein , results in dendritic spine immaturity and altered pre-synaptic function causing a reduction of paired-pulse facilitation . Oligophrenin-1 causes such effects through endocytosis of synaptic vesicles and post-synaptic AMPA receptor internalization [21, 22]. Therefore, it is possible that DIP/WISH-deficiency produces the observed abnormality through the regulation of endocytosis and AMPA receptor internalization, and a more detailed analysis in this aspect will be important.
In our study, the levels of various synaptic proteins such as PSD-95 and GluR2 were not drastically altered in DIP/WISH-KO mice (Figure 2C). In addition, the number of spines calculated in Golgi-stained samples did not differ between WT and DIP/WISH-deficient hippocampus (Figure 2D). Since spatio-temporal changes in spine morphology in DIP/WISH-deficient hippocampi are highly expected from the observed effects in behavioral and electrophysiological analyses, conventional analysis with Golgi-staining may simple be insufficient to detect the changes. More detailed analyses with high-resolution imaging methods such as two-photon laser-scanning microscopy may be necessary to determine the effects of DIP/WISH on spine formation. Such an analysis would make the interaction of Rho-mediated actin organization and spine formation clearer.
DIP/WISH deletion improves the performance in the Barnes maze test and increases hippocampal long-term potentiation in mice, an effect most likely due to synaptic changes associated with alterations in actin organization.
We generated DIP/WISH-KO mice and littermate WT control mice . DIP/WISH-KO mice and WT control littermates were obtained by breeding heterozygote mice. Mice were group housed (2 - 4 mice per cage) in a room with a 12-hr light/dark cycle (lights on at 7:00 am) with access to food and water ad libitum. Behavioral testing was performed between 9:00 am and 6:00 pm. After the tests, the apparati were cleaned with diluted sodium hypochlorite solution to prevent a bias due to olfactory cues.
Cell culture of dissociated hippocampal neurons
Mouse hippocampal neurons were prepared using a modified protocol from rat hippocampal culture methods . In brief, hippocampi were dissected from P0 pups and dissociated using trypsin (0.25%) and trituration. Neurons were plated on poly-D-lysine-coated coverslips (15 mm round, Assistant, Sondheim, Germany) at a final density of 1-5 × 105 cells/coverslips in Neurobasal Medium (Invitrogen, Carlsbad, CA) with B27 supplement (Invitrogen, Carlsbad, CA). After 12 hours, coverslips were immersed in astrocyte-conditioned medium (Neurobasal Medium with B27 supplement). To prevent overgrowth of glia, neuron cultures were treated with cytosine arabinoside (5 mM; Calbiochem, La Jolla, CA) after incubation for 3 days in vitro (DIV).
Immunoblotting (IB) was performed using a membrane fraction of cells as previously mentioned . For obtaining the membrane fraction, the cells were washed twice with ice-cold PBS containing 0.1 μM sodium orthovanadate (Na3VO4) and resuspended in TNE buffer (10 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, and complete EDTA-free protease inhibitor cocktail (PIC) (Roche), 1 μM Na3VO4). Samples were centrifuged for 15 min at 100,000 g. The pellets were then resuspended in TNE buffer with 1% Ipagel (NP-40) and sonicated for 30 s. Following centrifugation at 100,000 g for 30 min, protein concentrations of the supernatants were adjusted and they (200 μg) were subjected to IB. An anti-DIP/WISH antibody was made by us as shown previously . First antibodies for β-tubulinIII, β-actin, PSD-95 and GluR2 are from Sigma (St. Louis), Sigma, Upstate (Lake Placid) and Santa Cruz Biotech. (Santa Cruz), respectively.
In situ hybridization
Digoxigenin-labeled antisense/sense probes were used for in situ hybridization. Full coding region of mouse DIP cDNA was digested by BamHI and XhoI. The digested fragment was subcloned into pBluescriptSKII(+) BamHI and XhoI sites. After linearizing the plasmid (antisense: BamHI, sense: XhoI), digoxigenin-labeled antisense/sense probes were synthesized by RNA polymerase (antisense: T3 RNA polymerase, sense: T7 RNA polymerase). Detection of mRNA on cryo-sectioned tissues (14 mm) was performed by NBT/BCIP through alkaline phosphatase conjugated anti-DIG antibody (Roche).
Immunohistochemistry was performed as previously described . Briefly, adult mice (8 weeks old) were perfused with ice-cold 4% paraformaldehyde (PFA) into their left-ventricles. Brains were post-fixed with 4% PFA for 2 hours at 4°C, treated with 20% and 30% sucrose for several hours, embedded in OCT compounds (SAKURA, Tokyo, Japan), and then sectioned (10-14 μm thickness). The samples were pre-incubated with a blocking solution (3% bovine serum albumin and 0.3% Triton X-100 in phosphate-buffered saline (PBS)) for 1 hour, and then incubated overnight at 4°C in blocking solutions with rabbit polyclonal anti-DIP/WISH antibody (describe above). The signals were visualized with diaminobenzidine (DAB) staining by bright-field light microscopy.
For immunostainig of the dissociated hippocampal neurons, cells were incubated with anti-DIP/WISH and anti-MAP2 antibodies (Abcom, Cambridge, UK).
Golgi staining of hippocampal tissue from WT and DIP/WISH-KO mice was performed by using a section Golgi-impregnation procedure . The number of spines was calculated in Golgi-stained hippocampal samples and expressed per 15 μm dendritic length.
We prepared a group consisted of an equivalent number of DIP/WISH-KO mice and WT control littermates (8 - 12 weeks old, WT, KO; n = 15, 16) for the behavioral tests. All the behavioral tests were conducted in a manner similar to those described previously . All procedures involving the care and use of animals were carried out in accordance with institutional guidelines (National Institute for Physiological Sciences and the National Institute of Health guide for the care and use of laboratory animals and the Animal Care and Use Committee of Kyoto University Graduate School of Medicine). Raw data from the behavioral tests are shown in the mouse phenotype database (http://www.mouse-phenotype.org/).
The Barnes circular maze apparatus used was the one described by Takao et al. . This task is similar to the Morris water maze, as both tests require an escape response. The Barnes maze test was chosen for this study since it does not involve swimming, which is a required component of the Morris water maze test. The maze used a dry, white circular platform that was raised 75 cm above the floor, was 1.0 m in diameter, and connected 12 holes equally spaced around the perimeter (O'Hara & Co., Tokyo, Japan). A black Plexiglas escape box (17 × 13 × 7 cm), which had paper cage bedding on its bottom, was located under one of the holes. The hole above the escape box represented the target, similar to the hidden platform in the Morris task. The location of the target was consistent for a given mouse, but was randomized across mice. The maze was rotated daily, with the spatial location of the target unchanged with respect to the visual room cues, to prevent a bias based on olfactory or proximal cues within the maze. The first training of the first group was started when WT mice and DIP/WISH-KO mice were 18 - 23 weeks old. In order to familiarize mice with the maze and the existence of the escape box, they were subjected to two habituation sessions prior to the beginning of testing. Three trials per day were conducted for 7 successive days in the beginning. Distance, latency, and numbers of errors to reach the target hole were recorded. Genotype effects on these indices were analyzed by two-way repeated ANOVA. One day after the last training, a probe test was conducted without the escape box, to confirm that this spatial task was acquired based on navigation using distal environmental room cues. Mice were allowed to freely explore the maze for 3 min. Time spent around each hole was recorded. A single training trial was conducted immediately after the probe test. An additional probe test (retention test) was conducted one week after the last training trial. Latency to reach the target hole and time spent around each hole were recorded by video tracking software (Image BM; see ref. ). One week after the memory retention test, the escape box was moved to a new position opposite to the original (reversal learning). Mice were then tested on five successive days to locate the new position of the escape hole using the same procedure as described above.
Preparation of hippocampal slices
The preparation of brain slices has been previously described . DIP-deficient and wild-type littermate mice (8-weeks old, backcrossed more than 7 times) were sacrificed using cervical dislocation, after which the brain was extracted quickly from the skull utilizing a curved spatula. The extracted brain was then transferred to ice-cold ACSF containing (in mM) 120 NaCl, 2.5 KCl, 1.3 MgSO4, 1.0 NaH2PO4, 26 NaHCO3, 2.5 CaCl2, and 11 D-glucose (where it was kept for a period of 1-2 minutes). Following this, the brain was then moved to a vibratome (752 M Vibroslice, Campden Instruments, Lafayette, IN, U.S.A.) where hippocampal slices (400 μm) were sectioned and collected in the presence of ice-cold ACSF. The slices were then allowed to recover in a submerged holding chamber bubbled with carbogen (95% O2/5% CO2). The time of recovery was at least 1 hour before the initiation of recording experiments.
Extracellular fEPSP electrophysiological recordings
After a recovery period of at least 1 hour in the submerged holding chamber, a single slice was subsequently transferred to the recording chamber where it underwent submersion and superfusion with 95% O2-5% CO2 saturated artificial cerebral spinal fluid (ACSF, 2 ml/min) at a controlled temperature of 28°C. The ACSF contained (in mM) 120 NaCl, 2.5 KCl, 1.3 MgSO4, 1.0 NaH2PO4, 26 NaHCO3, 2.5 CaCl2, and 11 D-glucose. For recording of field EPSPs, the glass recording pipette (3 MΩ) was filled with ACSF solution.
Synaptic responses were evoked by bipolar tungsten electrodes located 200-400 μm from the cell body layer of the hippocampal CA1 region. fEPSPs were measured by utilizing the rising phase slope between 5% and 60% of the peak response. All data acquisition and analysis were achieved through use of the pCLAMP 7 software (Axon instruments). Paired-pulse facilitation (PPF) was recorded with the first and second responses being separated by a range of stimulation intervals. The ratio between the magnitudes of the slope of the succeeding response to the slope of the initial response was then calculated. For LTP studies, a high-frequency stimulation (HFS) induction protocol using 2 trains of 100 Hz at 10 second intervals (with each train lasting 1s) was utilized following a stable baseline period. n corresponds to the specific number of hippocampal slices used in each experimental group. Customarily, one slice was used per mouse during experiments. For representation of average data, data underwent normalization to the average of the baseline responses unless otherwise stated. The shown representative traces of fEPSPs were averages of five successive sweeps during experimental recordings. Representative traces for PPF studies were obtained at the 100 ms interval. For assessment of the LTP size, the means of potentiation during the last 5-10 minutes of field recordings in both wild-type and mutant groups were compared statistically by the use of Student's t-test. For analysis of basal synaptic strength, experimental results were examined by comparing the recorded means at the indicated stimulation intensities between wild-type and mutant groups through Student's t-test. For PPF studies, data obtained at the stated interpulse interval recordings were weighed between wild-type and mutants via Student's t-test. In all the above cases, a P-value was obtained where P < 0.05 was considered significant. All experimental protocols were approved by The Hospital for Sick Children Animal Care Committee.
Test of auditory function
We used ABR testing to evaluate auditory thresholds. DIP/WISH-KO and WT mice at 12 weeks of age were anesthetized with an i.p. injection of pentobarbital sodium (Nembutal), and anesthesia was supplemented during the course of measurement as necessary. The electrodes were subcutaneously inserted at the vertex (positive), left or right ear (mastoid; negative) and opposite mastoid (ground). In a calibrated, closed sound system, broadband click stimuli were presented at levels from 0 - 80 dB sound pressure level (SPL). Evoked responses were amplified (x10k), filtered with a band pass of 200 Hz to 3 kHz and averaged with 500 sweeps. Typical ABR waveforms evoked at increasing stimulus levels are shown in Figure 6. Experimental protocols were approved by The Hospital for Sick Children Animal Care Committee.
Scanning electron microscopy
Inner eras were fixed in 2.5% glutaraldehyde in PBS for at least 1 hour. The sensory areas were microdissected, dehydrated in graded ethanol solutions and critical point-dried from liquid CO2. Procedures for scanning electron microscopy were as described .
This work was supported by grants to TF-T and MT from the Ministry of Education, Culture, Sports, Science. We thank Dr. Yugo Fukazawa (National Institute for Physiological Science, Japan) for his assistance for Golgi-staining.
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