- Open Access
TRPC1 mediates slow excitatory synaptic transmission in hippocampal oriens/alveus interneurons
Molecular Brain volume 13, Article number: 12 (2020)
Hippocampal GABAergic interneurons play key roles in regulating principal cell activity and plasticity. Interneurons located in stratum oriens/alveus (O/A INs) receive excitatory inputs from CA1 pyramidal cells and express a Hebbian form of long-term potentiation (LTP) at their excitatory input synapses. This LTP requires the activation of metabotropic glutamate receptors 1a (mGluR1a) and Ca2+ entry via transient receptor potential (TRP) channels. However, the type of TRP channels involved in synaptic transmission at these synapses remains largely unknown. Using patch-clamp recordings, we show that slow excitatory postsynaptic currents (EPSCs) evoked in O/A INs are dependent on TRP channels but may be independent of phospholipase C. Using reverse transcription polymerase chain reaction (RT-PCR) we found that mRNA for TRPC 1, 3–7 was present in CA1 hippocampus. Using single-cell RT-PCR, we found expression of mRNA for TRPC 1, 4–7, but not TRPC3, in O/A INs. Using co-immunoprecipitation assays in HEK-293 cell expression system, we found that TRPC1 and TRPC4 interacted with mGluR1a. Co-immunoprecipitation in hippocampus showed that TRPC1 interacted with mGluR1a. Using immunofluorescence, we found that TRPC1 co-localized with mGluR1a in O/A IN dendrites, whereas TRPC4 localization appeared limited to O/A IN cell body. Down-regulation of TRPC1, but not TRPC4, expression in O/A INs using small interfering RNAs prevented slow EPSCs, suggesting that TRPC1 is an obligatory TRPC subunit for these EPSCs. Our findings uncover a functional role of TRPC1 in mGluR1a-mediated slow excitatory synaptic transmission onto O/A INs that could be involved in Hebbian LTP at these synapses.
Hippocampal GABAergic interneurons represent a diverse population of inhibitory cells that are involved in gating information flow and computations by controlling principal cells activity [1, 2]. Interneuron functions are not static and inhibitory cells express short- and long-term plasticity of their synaptic inputs and outputs [3, 4]. Long-lasting changes at interneurons synapses may serve to enhance hippocampal network computation and flexibility [3, 5]. O/A INs are dendrite-projecting interneurons consisting mostly of oriens/lacunosum-moleculare (O-LM) cells, but also projection cells with additional subicular, retro-hippocampal or septal projections, as well as bistratified cells [2, 6]. O/A INs receive excitatory glutamatergic inputs from CA1 pyramidal cells that express a Hebbian form of LTP [5, 7, 8]. This LTP depends on the activation of metabotropic glutamate receptor subtype 1a (mGluR1a) and postsynaptic calcium elevation [9, 10]. At the circuit level, LTP of O/A IN input synapses regulates metaplasticity of CA1 pyramidal cells  and hippocampal long-term memory consolidation  .
High frequency stimulation of excitatory inputs onto O/A INs elicit slow excitatory postsynaptic currents (EPSCs) that are mediated by mGluR1a and non-selective cationic channels of the transient receptor potential (TRP) family . Moreover, Ca2+ influx via TRP channels following mGluR1a activation is necessary for LTP induction at O/A IN excitatory synapses . However, which TRP channel is involved in transmission and plasticity at these synapses remains unknown.
The canonical TRP (TRPC) subfamily contains seven members (TRPC1 to TRPC7) that form non-selective cation channels. TRPCs are highly expressed in brain including the cerebral cortex, hippocampus, cerebellum, and amygdala [14,15,16,17,18]. These channels play important role in neuronal development , synaptic plasticity  and memory [21, 22]. Deficits in TRPC function have been suggested to contribute to brain disorders like autism spectrum disorders, intellectual disability and bipolar disorder . In the hippocampus, TRPC can be activated by different G-protein coupled receptors like tyrosine kinase receptors, mGluR1/5 and muscarinic cholinergic receptors usually through phospholipase C pathway engagement [24,25,26,27].
A number of studies have reported the involvement of TRPCs in mGluR1a-mediated synaptic transmission [28,29,30,31,32,33]. TRPC1 was reported to physically associate with mGluR1a receptors in the cerebellum and dopaminergic neurons of the substantia nigra (SN) [29, 34]. However other reports suggest also an implication of TRPC3 in cerebellar mGluR1a-mediated slow transmission [32, 35]. The presence of TRPCs is well documented in the hippocampus [16,17,18, 36,37,38,39,40,41], but their role in inhibitory interneurons remains largely unaddressed.
In this work, we examined in more detail the role of TRPCs in excitatory synaptic transmission in O/A INs. We found that O/A INs express mRNA for TRPC1, 4–7, and that TRPC1 was co-localized with mGluR1a in O/A IN dendrites. Moreover, siRNA knock-down of TRPC1, but not TRPC4, impaired slow EPSCs in O/A INs. Thus, TRPC1 is a necessary component of mGluR1a-mediated slow excitatory synaptic transmission in O/A INs, uncovering a possible role in induction of Hebbian LTP at these synapses.
Materials and methods
Acute hippocampal slices
Hippocampal acute slices were prepared from 3 to 4 weeks old Sprague-Dawley rats. Animals were anesthetized with isoflurane inhalation and the brain was rapidly removed and placed in ice-cold sucrose-based cutting solution containing (in mM): 250 sucrose, 2 KCl, 1.25 NaH2PO4, 7 MgSO4, 0.5 CaCl2, 26 NaHCO3, 10 glucose, pH 7.4, and 300 mOsmol/L. A block of tissue containing the hippocampus was prepared and 300 μm transverse hippocampal slices were obtained with a Leica VT1000S vibratome. Slices were transferred for recovery for 1 h at room temperature in artificial cerebral spinal fluid (ACSF) containing (in mM): 124 NaCl, 2.5 KCl, 1.25 NaH2PO4, 1.3 MgSO4, 2.5 CaCl2, 26 NaHCO3, and 10 glucose (pH 7.3–7.4, 295–305 mOsmol/L). Both cutting solution and ACSF were saturated with 95% O2/5% CO2.
Organotypic hippocampal slice culture and biolistic transfection
Organotypic hippocampal slice cultures were prepared from 7 to 12 days old rats and biolistically co-transfected with a plasmid for EYFP expression and siRNAs as previously described . Slices were transfected after 3 days in vitro and were used for experiments 2 days later. All small interfering RNAs (siRNAs) were purchased from Dharmacon (Lafayette, CO, USA). Commercial siControl was used as non-targeting control siRNA. TRPC1 siRNA was a combination of four target sequences whereas TRPC4 was a single sequence. All sequences are listed in Table 1.
Hippocampal slices were perfused continuously with ACSF (2.5 ml min−1, 31–33 °C). CA1 interneurons located in stratum oriens/alveus were visually identified using an upright microscope (Nikon Eclipse, E600FN) equipped with a water-immersion long-working distance objective (40x, numerical aperture 0.8) and an infrared video camera (70 Series, DAGE-MTI, MI, USA). EYFP-expressing cells were identified using an X-cite 120 arc lamp (EXFO photonic solution Inc., Mississauga, ON, Canada). Whole-cell voltage-clamp recordings were obtained using borosilicate glass pipettes (4–5 MΩ, WPI, Inc., FL, USA) filled with intracellular solution containing (in mM): 130 CsMeSO3, 5 CsCl, 2 MgCl2, 10 HEPES, 10 EGTA, 5 Na2- phosphocreatine, 2 ATP-Tris, 0.4 GTP-Tris, 1 QX314, pH 7.2–7.3, and 280 ± 5 mOsmol. Data was acquired using a Multiclamp 700A amplifier (Molecular Devices, CA, USA) and digitized using Digidata 1440A and pClamp 10 (Molecular Devices). Recordings were low-pass filtered at 2 kHz and digitized at 10 kHz. Series resistance was regularly monitored during experiments and data were included only if the holding current and series resistance were stable. To overcome potential biological variability and confirm experimental validity, in acute slice experiments, recordings were routinely obtained from only one cell per slice and repeated at least in three animals. For cultured slice experiments, recordings were obtained from only one cell per slice and from at least three independent culture experiments.
To isolate mGluR1a-mediated excitatory postsynaptic currents (mGluR1a-EPSCs), antagonists of non-NMDA (6-cyano-7-nitroquinoxaline-2,3-dione; CNQX, 20 μM), NMDA (DL-2-amino-5-phosphonopentanoic acid; AP5, 50 μM), and GABA-A (gabazine, 5 μM) receptors (all from Sigma, Oakville, ON, Canada), as well as the glutamate transporter blocker DL-threo-b-benzyloxyaspartic acid (TBOA, 30 μM) (Tocris, Ellisville, MO,USA) were bath-applied. In some experiments, the mGluR1a receptor antagonist (S)(+)-α-amino-4-carboxy-2-methylbenzeneacetic acid (LY367385, 100 μM), the TRP channel antagonist 1–2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)proproxy]ethyl-1H-imidazole (SKF96365, 30 μM) (both drugs from Tocris), or the phospholipase C inhibitor 1-[6-[[(17β)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U73122, 10 μM) (Calbiochem, Gibbstown, NJ, USA), were added to the external solution.
Rat brains were removed and dissected in Hank’s balanced salt solution (HBSS; Invitrogen, Carlsbad, CA, USA). Cortico-hippocampal slices (600 μm thick) were obtained using a McIlwain tissue chopper (Campen Instruments, Lafayette, IN, USA) and CA1 region was surgically isolated under a dissecting microscope. Total RNA was extracted from 20 mg of CA1 tissue with a GenElute Mammalian Total RNA Miniprep kit (Sigma). Reverse transcription was performed with 5 μg of CA1 extracted RNA using M-MLV reverse transcriptase (Invitrogen) following manufacturer’s protocol. All procedures were performed under RNase free conditions. For each member of the TRPC family, cDNA was amplified by PCR in separate reactions. Two microliters of the reverse transcription product were mixed with 0.1 μM of each primer, 1 U of Taq DNA polymerase (NEB), 0.1 mM of each dNTP, and 2 mM MgSO4 and amplified for 35 cycles using the following program: 93 °C, 30 s; 55 °C, 30 s; 72 °C, 45 s. Sequences of PCR primers and expected product sizes are listed in Table 2. Amplification of genomic DNA was excluded by intron-overspanning location of all primers.
Multiplex single-cell RT-PCR
After whole-cell patch-clamp recording in acute slices, O/A interneuron cytoplasm was removed by applying gentle negative pressure to the patch pipette for 10 min. After withdrawal of the pipette from the slice, pipette solution (5–6 μl) was expelled into a microtube containing the reverse transcriptase mixture: 20 U RNase OUT (Invitrogen) and 10 mM DTT (12 μl final volume). Reverse transcription procedure was carried out as described above. cDNAs were then amplified by a two-step PCR. For the first round PCR, the whole reverse transcription product was separated in 2 tubes: the first one containing primers for TRPC1, 4, 5, 7 and the other containing primers for TRPC3, 6 and glial fibrillary acidic protein (GFAP). Each tube contained 0.1 μM of each primers, 2.5 U of taq DNA polymerase, 2 mM MgSO4 and no dNTP were added (50 μL of final volume). PCR was run as described above for 22 cycles. For the second round PCR, amplifications were performed in seven separate reactions. Each reaction contained: 2.5 μl of the appropriate first round PCR product, 1 μM of each primer, 1 U of Taq DNA polymerase, 0.1 mM of each dNTP and 2 mM MgSO4 (25 μl final volume). PCR was run as described above for 35 cycles. For TRPC3 and GFAP, the second round PCR required semi-nested and nested PCR, respectively. The sequences of internal primers are listed in Table 2.
PCR products were visualized on an ethidium bromide-stained agarose gel. Positive controls using 100 ng of total CA1 RNA were carried out in parallel for each single-cell amplification. GFAP primers were added as a control for glial RNA contamination and only cells that were GFAP negative were kept for data analysis. Controls for other contamination artefacts were performed by advancing a pipette into the slice without aspiration.
Isolated hippocampus was homogenized (Polytron, 3 × 2 s, setting 4) (Glenn Mills, Clifton, NJ, USA) in ice-cold buffer containing: phosphate buffer saline (PBS), 1% Triton X-100, 1 mM NaF, 1 mM Na3VO4 and protease inhibitors (Cocktail inhibitor set I; Calbiochem). Homogenate was centrifuged at 20000 g for 20 min at 4 °C and the resulting supernatant was assayed for protein concentration using BCA (Pierce, Rockford, IL, USA). One milligram of proteins was incubated with 2 μg of rabbit polyclonal anti-TRPC1 (Sigma) for 30 min at 4 °C. The specificity of the immunoprecipitation was controlled by reabsorption of the antibody with equivalent amount of the antigen peptide overnight at 4 °C before incubation with tissue homogenate. Immune complexes were precipitated with 50 μl of 50% protein-G sepharose (Sigma) slurry for 90 min at 4 °C. The beads were washed three times for 10 min in PBS-1% Triton X-100 and proteins were eluted by the addition of electrophoresis sample buffer and heated at 65 °C for 7 min. Western blot detection was as previously described  using rabbit polyclonal anti-mGluR1a (1/500; Upstate Biotechnology, Lake Placid, NY, USA) and HRP-conjugated anti-rabbit IgG (1/10000; Jackson Immunoresearch, West Grove, PA, USA).
HEK-293 cells were grown and transfected as described previously . In brief, cells were cultured in 6-well dishes and transfected with 1 μg of pRK5-mGluR1a (from Dr. J.P. Pin, Montpellier, France), and 1 μg of pcDNA3.1-FLAG-TRPC1 (from Dr. C. Montell, Baltimore, USA) or pcDNA3.1-myc-TRPC4 (from Dr. L. Méry, Paris. France) constructs. Two days after transfection, cells were collected in lysis buffer containing: 50 mM Tris pH 7.4, 120 mM NaCl, 1% Triton X-100, 2 mM EDTA, 1 mM NaF, 1 mM Na3VO4 and protease inhibitors. One well of confluent cells were used for each immunoprecipitation assay. Cleared supernatants were incubated with 2 μg of anti-FLAG (Sigma) or anti-mGluR1a. The rest of the procedure was the same as described above. The specificity of the immunoprecipitation was controlled by omitting to transfect the construct coding the immunoprecipitated protein. Western blot detection was performed using rabbit polyclonal anti-mGluR1a or mouse monoclonal anti-myc (1/5000, Sigma) and HRP-conjugated anti-rabbit or anti-mouse IgGs.
TRPC1 and mGluR1a double-label immunofluorescence
Eighteen to twenty-one days old rats were deeply anaesthetized with intra-peritoneal injection of sodium pentobarbital (MTC Pharmaceuticals, Cambridge, Ontario, Canada) and transcardially perfused with a fixative solution containing 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4). The brain was dissected, postfixed for 4 h at room temperature and cryoprotected overnight in 30% sucrose. Transverse sections (45 μm thickness) were cut on a freezing microtome (Leica SM 2000R, Germany). Free floating sections were permeabilized 15 min in PBS-0.2% Triton X-100 and blocked 1 h at room temperature in PBS containing 10% NGS and 0.1% Triton X-100. Sections were incubated 4 days at 4 °C in a mixture of mouse monoclonal anti-mGluR1a (1/100 BD BioSciences, Oakville, ON, Canada) and rabbit polyclonal anti-TRPC1 (1/250) or rabbit anti-TRPC4 (1/100; Alomone Labs, Jerusalem, Israel). Sections were incubated 1 h 30 min at room temperature in biotinylated goat anti-rabbit IgG (1/200, Jackson ImmunoReasearch) and overnight at 4 °C in a mixture of Streptavidin-Alexa Fluor 488 (1/200, Invitrogen) and Texas Red conjugated goat anti-mouse (1/200; Jackson ImmunoResearch). Sections were rinsed thoroughly between incubations. All antibodies were diluted in PBS containing 2% NGS and 0.1% Triton X-100 (0.01% NaN3 was added for primary antibodies). Sections were mounted in ProLong Gold antifade reagent (Invitrogen) and observed with a Zeiss LSM 510 confocal microscope system (Axioskop; Coherent, Santa Clara, CA, USA) using appropriate filters.
For siRNA efficacy assessment, slices were homogenized by sonication in 50 μl of homogenization buffer containing: 50 mM Tris pH 7.4, 120 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM NaF, 1 mM Na3VO4 and protease inhibitors. The homogenate was then centrifuged at 20000 g for 20 min and protein concentration in the supernatant was determined as described above. Fifty micrograms of proteins per condition were subjected to western blot analysis as previously described . Membrane was horizontally cut (~ 60 kDa molecular weight) to separate higher and lower molecular weight proteins. Top membrane was probed with rabbit polyclonal anti-TRPC1 (1/200) while bottom membrane was probed with mouse monoclonal anti-actin (1/1000, Santa Cruz Biotechnology, Santa Cruz, CA). After immunodetection, top membrane was stripped and re-probed with anti-TRPC4 (1/1000; Alomone; Jerusalem, Israel). Immunoreactive bands were scanned with a desktop scanner and quantified with Quantity One software (Bio-Rad, Hercules, CA, USA). The optical density of TRPC1 or TRPC4 was normalized to actin. Slices transfected with TRPC1 or TRPC4 siRNA were compared with slices transfected with scrambled siRNA. Only slices with comparable level of transfection, based on EYFP expression, were kept for analysis.
Summary data are expressed as mean ± s.e.m. Statistical significance was determined using two-tailed Student’s t tests for 2 groups comparisons (paired and unpaired comparisons indicated in text), or ANOVA followed by Bonferroni pair-wise comparisons for multiple groups, as appropriate. Significance level was set at p < 0.05.
Slow EPSCs in O/A INs are mediated by TRP channels and independent of PLC signalling
Brief high-frequency stimulation (HFS) of hippocampal CA1 stratum oriens elicits in O/A INs a slow excitatory postsynaptic current (slow EPSC) mediated by mGluR1a and TRP channels . Activation of group I mGluRs (mGluR1/5) by the selective agonist DHPG triggers membrane depolarization in CA1 O/A INs that is independent of PLC signalling . However, the implication of PLC in slow EPSCs of O/A INs has not been investigated. Slow EPSCs were evoked in CA1 O/A INs of acute slices by HFS preceded by a brief depolarisation (to allow Ca2+ influx through voltage-gated Ca2+ channels) in the presence of TBOA (DL-threo-b-benzyloxyaspartic acid) (30 μM), a blocker of glutamate transporters (to enhance glutamate spillover to perisynaptic mGluR1a), as previously described . In these conditions, HFS elicited slow EPSCs in O/A INs (Fig. 1 a). The amplitude of slow EPSCs was significantly reduced (41 ± 12% of control, p < 0.05, unpaired t-test) in the presence of the TRP channel antagonist SKF96365 (30 μM) and partially recovered after its washout (61 ± 12% of control). Slow EPSCs were not affected in the presence of the PLC inhibitor U73122 (10 μM) (Fig. 1 b, 109 ± 4% of control, p > 0.05, paired t-test), suggesting that slow EPSCS in O/A INs are mediated by TRP channels but may be independent of PLC signalling.
We then tested if slow EPSCs are present in CA1 O/A INs of hippocampal organotypic slices. In similar conditions as above, HFS evoked slow EPSCs in O/A INs in cultured slices (Fig. 1 c). Previous report showed that stimulation of mGluR1/5 in acute slices elicited only minimal membrane depolarisation and calcium transients in other CA1 interneurons located in stratum radiatum/lacunosum-moleculare . Thus, we examined if slow EPSCs are elicited also in other interneurons in stratum radiatum in cultured slices. We found that HFS only elicited EPSCs with much smaller amplitude in stratum radiatum interneurons (Fig. 1 c; p < 0.05). Overall, these results suggest that slow EPSCs in CA1 O/A INs may be cell-type specific, mediated by TRP channel activation and independent of PLC signalling.
TRPC mRNA expression in hippocampal CA1 region and in single O/A INs
Among the TRP channel superfamily, TRPCs are a subfamily of channels that are highly expressed in brain and are associated with slow excitatory transmission [29, 32, 35, 45, 46]. Thus, we set out to examine the role of TRPCs in mGluR1a-mediated slow EPSCs in hippocampal O/A INs. So first, we determined mRNA expression of the seven TRPC members (TRPC1–7) in hippocampal CA1 region. Reverse transcriptase polymerase chain reaction (RT-PCR) detection of TRPC mRNAs was performed on total RNA isolated from microdissected CA1 region. We found that mRNAs for TRPC1, 3, 4, 5, 6 and 7 are expressed in CA1 region (Fig. 2 a).
Next we determined which TRPC mRNAs are expressed specifically in CA1 O/A INs. We performed simultaneous detection of the six TRPC mRNAs detected in CA1 region (TRPC1, 3, 4, 5, 6 and 7). After whole-cell recording of slow EPSCs in O/A INs, the cytoplasm was harvested into the patch pipette and multiplex single-cell RT-PCR was performed. Except for TRPC3, all other TRPC (TRPC1, 4, 5, 6 and 7) mRNAs present in CA1 region were detected in single O/A INs (Fig. 2 b). In additional whole cell recordings from O/A INs in which slow EPSCs was not tested (n = 3 cells), TRPC3 mRNA was not detected either. Different combinations of TRPC1, 4, 5, 6 and 7 mRNAs were observed in individual cells (ex. Fig. 2 c) but never all transcripts together. These results suggest that individual CA1 O/A INs express mRNA for TRPC1, 4, 5, 6 and 7, but not TRPC3.
Molecular interaction of mGluR1a with TRPC1 and 4 in HEK-293 cells, and with TRPC1 in hippocampus
In cerebellum, TRPC1 and 3 are implicated in slow mGluR1-mediated EPSCs [29, 32, 33, 35], and mGluR1a and TRPC1 physically interact supporting an mGluR1a activation of TRPC1 . Since we did not find evidence of TRPC3 expression in O/A INs, we focused on TRPC1 and TRPC4, another TRPC susceptible to act in slow synaptic transmission [29, 47]. To determine if mGluR1a associates with TRPC1 and TRPC4, we first performed co-immunoprecipitation assays in HEK-293 cells transiently transfected with mGluR1a and FLAG-tagged TRPC1, or myc-tagged TRPC4. Immunodetection of mGluR1a following TRPC1 immunoprecipitation with a FLAG antibody (Fig. 3 a), indicated a molecular interaction between mGluR1a and TRPC1. No signal was detected when TRPC1 was not transfected (data not shown). Similarly, immunodetection of myc-tagged TRPC4 after mGluR1a immunoprecipitation (Fig. 3 a), showed an interaction between mGluR1a and TRPC4. No signal was detected when mGluR1a was not transfected (data not shown).
Next, we examined whether these interactions occur in vivo by carrying out immunodetection of mGluR1a after immunoprecipitation of TRPC1 from hippocampal extracts (Fig. 3 b). We found that mGluR1a co-immunoprecipitated with TRPC1 and this was inhibited by preadsorption of the antibody with a blocking peptide (Fig. 3 b). Similar experiments with a TRPC4 antibody, failed to find mGluR1a co-immunoprecipitation with TRPC4 (data not shown). Our data suggest a molecular interaction of mGluR1a with TRPC1 and TRPC4 in recombinant system, but with TRPC1 in the hippocampus.
TRPC1 and TRPC4 co-localize with mGluR1a in O/A INs
Next we examined the cellular co-localization of mGluR1a with TRPC1 or TRPC4 in hippocampal CA1 O/A INs using confocal microscopy and double-label immunofluorescence with antibodies against mGluR1a and either TRPC1 or TRPC4 (Fig. 4). TRPC1 antibody labelled neuronal processes in stratum oriens/alveus as well as cell body of interneurons, consistent with previous report . In some cases, O/A interneuron dendrites were distinguishable from surrounding processes and these dendrites were positive for TRPC1 (Fig. 4 a-b). The mGluR1a antibody also labelled O/A INs and their dendrites (Fig. 4 a-b, middle), consistent with previous data . Superimposition of the two labels showed co-localization of mGluR1a and TRPC1 in cell bodies and dendrites of O/A INs (Fig. 4 a-b). TRPC4 immunolabelling was also present in O/A INs but appeared limited to the cell body (Fig. 4 c). TRPC4 also co-localized with mGluR1a (Fig. 4C). These results show that TRPC1 has an overlapping distribution with mGluR1a in the cell body and dendrites of O/A INs while TRPC4 co-localization is predominant in the cell body.
TRPC1, but not TRPC4, mediates slow EPSCs in O/A INs
Next we examined whether TRPC1 or TRPC4 contributes to mGluR1a-dependent slow EPSCs in CA1 O/A INs using biolistic transfection of small interfering RNA (siRNA) in cultured hippocampal slices. First, we validated the specificity and efficacy of siRNAs targeting TRPC1 and TRPC4 by western blot. Transfection of siRNA targeting TRPC1 (siTRPC1) (Fig. 5) reduced expression of TRPC1 relative to slices transfected with scrambled siRNA (siCTL) (59 ± 13% of control; p < 0.05), without affecting TRPC4 expression (92 ± 20% of control; p > 0.05; n = 3). Conversely, transfection of siRNA targeting TRPC4 (siTRPC4) reduced TRPC4 expression (67 ± 10% of control; p < 0.05), but not TRPC1 (100 ± 17% of control; p > 0.05). Since mostly the superficial parts of slices are transfected with biolistic transfection in our conditions  and entire slices are used for western blot, the efficacy of siRNA is probably underestimated in our experiments. Nonetheless, our results confirm the specific protein knockdown of the respective target of siRNAs.
Next, we co-transfected cultured slices with siRNA and EYFP, and used whole cell recordings from EYFP-expressing O/A INs to determine the role of TRPC1 and 4 in slow EPSCs (Fig. 6). In O/A INs transfected with siTRPC1, the amplitude of slow EPSCs was drastically reduced compared to EPSCs in O/A cells transfected with siCTL (Fig. 6 b-c; 4 ± 3% of control, p < 0.05). In contrast, in O/A INs transfected with TRPC4 siRNA slow EPSCs were not affected (Fig. B-C; 112 ± 18% of control; p > 0.05). To rule out unspecific effect of siRNAs on glutamatergic synaptic transmission, we recorded in the same O/A INs fast ionotropic glutamate receptor-mediated EPSCs evoked by single stimulus before bath application of CNQX and AP-5. Transfection of siTRPC1, siTRPC4 or siCTL did not affect these fast EPSCs (Fig. 6 b, left traces). Our results indicate that TRPC1, but not TRPC4, mediates slow EPSCs in O/A INs.
In this work, we examined the role of TRPCs in slow excitatory synaptic transmission in hippocampal O/A interneurons. We found that O/A INs express mRNA for TRPC1, 4–7, and that TRPC1 was co-localized with mGluR1a in O/A IN dendrites. Moreover, siRNA knock-down of TRPC1, but not TRPC4, impaired slow EPSCs in O/A INs. Thus, TRPC1 is a necessary component of mGluR1a-mediated slow excitatory synaptic transmission in O/A INs, and, consequently, may be involved in induction of Hebbian LTP at these synapses.
TRPC expression and association with mGluR1a
TRPC channels are widely expressed in brain and they are known to mediate slow inward mGluR-dependent currents in many cell types [12, 13, 29,30,31,32,33, 35, 50]. To date, few pharmacological tools allows blockade of specific members of this family [51, 52]. Thus, studies that look at the expression and molecular interactions of TRPC channels with mGluRs are useful for the identification of TRPC members involved in this form of synaptic transmission.
RT-PCR analysis of TRPC expression revealed that, except for TRPC2, all TRPC transcripts are present in the CA1 region of hippocampus. These results are consistent with previous studies in rodents [16, 17, 36, 38,39,40,41]. However, these studies mostly focused on pyramidal cells and none of them specifically examined TRPC expression in interneurons. Single-cell RT-PCR revealed that O/A INs express TRPC1, 4, 5, 6, and 7 mRNAs in various combinations. The fact that we were unable to detect TRPC3 suggests that this member of TRPC family is absent or less common in O/A INs.
Immunofluorescence labelling showed that TRPC1 protein is expressed in interneurons of stratum oriens/alveus, consistent with previous report . Our results show more precisely that TRPC1 is present in the cell body and dendrites of O/A INs where it co-localizes with mGluR1a. Such overlapping localization was also shown in midbrain dopamine neurons  and in auditory midbrain neurons , as well as in cerebellar Purkinje cells that display mGluR1a-mediated EPSCs . Moreover, previous study reports a physical interaction between TRPC1 and mGluR1a in recombinant systems and in cerebellum . Here, we show that this interaction between these two proteins is also present in the hippocampus. Taken together, our findings indicate the presence of both mGluR1a and TRPC1 in somato-dendritic compartment of O/A INs, a co-localization consistent with their functional interaction underlying EPSCs.
O/A INs also express TRPC4 protein, as shown previously . However, in contrast to TRPC1, its immunolocalization appears limited to O/A IN cell bodies, and no molecular interaction was observed between TRPC4 and mGluR1a in the hippocampus. These results are in agreement with our finding using siRNA knock-down that TRPC4 is not required for slow EPSCs in O/A INs. TRPC1 was previously shown to be the only TRPC member to interact with mGluR1a in CHO cells . However, in our hands, TRPC4 associates with mGluR1a in HEK-293 cells. This discrepancy could be explained by the variable levels of expression in heterologous systems.
TRPC1 is involved in slow EPSCs in O/A interneurons
Brief high frequency stimulation induces slow inward mGluR1a-mediated EPSCs in O/A INs . Pharmacological activation of mGluR1a in O/A INs leads to dendritic Ca2+ currents via TRP channels . In the present work, we demonstrated that slow EPSCs are specific to CA1 O/A INs and are absent in stratum radiatum interneurons, and that TRPC1 mediates the slow mGluR1a-mediated EPSCs in O/A interneurons. Our findings are consistent with previous evidence that TRPC1 is involved in mGluR1a-mediated EPSCs in cerebellar Purkinje cells and GABAergic molecular layer interneurons [29, 33].
Slow EPSCs in O/A INs showed considerable cell-to-cell variability (Fig. 1 a-c). Such variability across individual cells may be explained by the presence of multiple cell classes in that region. However, in the original paper describing these slow EPSCs, similar high variability in response magnitude was reported and intracellular labelling identified the recorded cells as O-LM cells . Thus, the reason for this variability remains unclear but it may not necessarily be due to recording from multiple cell classes.
Functional TRPC channels are formed of four subunits  and different TRPCs co-assemble in vivo to form heterotetrameric channels with properties distinct from homomeric channels [17, 22]. TRPC4 and TRPC5 were found to associate with TRPC1 in brain synaptosomes  and in the hippocampus . In pyramidal cells of the lateral amygdala, mixed mGluR1/5-mediated EPSCs involve both TRPC1 and TRPC5 . In layer 5 of prefrontal cortex, agonist activation of mGluR1/5 receptors induces currents in neurons with strong expression of TRPC4 and TRPC5 mRNAs . In the present work, despite the co-localization of TRPC4 and mGluR1a in CA1 O/A INs, siRNA knock-down of TRPC4 did not affect slow EPSCs in these cells, suggesting an absence of functional interaction between TRPC4 and TRPC1 during slow EPSCs in O/A INs. However, our results do not exclude the possible involvement of other TRPC members (TRPC5–7) expressed in O/A interneurons, in addition to TRPC1, in mediating slow excitatory synaptic transmission in these cells.
mGluR1a activation of TRPC1
Mechanisms underlying mGluR1a activation of TRPCs are still largely unknown. Here, our evidence suggests that TRPC-mediated slow EPSCs may be independent of the PLC pathway classically linked to group I mGluRs. In cerebellar Purkinje cells and GABAergic interneurons, as in midbrain dopamine neurons, mGluR1a-mediated EPSCs are also PLC-independent [33, 56,57,58] whereas in lateral amygdala mGluR1/5-mediated currents are PLC-dependent . Thus, different mechanisms may link mGluR activation of TRPCs in a cell-specific manner.
Modulation of mGluR1a-induced currents and dendritic calcium transients by Src family tyrosine kinases and extracellular signal-regulated kinase (ERK) was reported in O/A INs . Interestingly, in cerebellar Purkinje cells and GABAergic interneurons, as well as in midbrain dopamine neurons, PLC-independent mGluR1a-mediated currents also show tyrosine kinase modulation [33, 57, 58]. Thus, it will be interesting to investigate the role of these kinases in gating or modulating TRPC1 and slow EPSCs in O/A INs.
Stromal interacting molecule 1 (STIM1), a calcium sensor protein of the endoplasmic reticulum, was shown to gate TRPC1 channel under basal condition and upon receptor stimulation in different systems [59,60,61,62]. Activation of the channel was dependent on a lysine-rich region in the cytoplasmic domain of STIM1. Orai1 and caveolin were proposed to be partners with STIM1 in the activation of TRPC1, but the exact mechanisms of this gating are still under investigation [63, 64]. TRPC channels are also gated by conformational coupling with inositol-1,4,5-triphosphate receptors (IP3Rs) [65, 66] and ryanodine receptors (RyRs) . This coupling is thought to be regulated by Homer proteins , a family of proteins that also interact with mGluR1a . Since STIM1, Homer and TRPC have been shown, separately, to be implicated in mGluR dependent synaptic plasticity in Purkinje cell and hippocampus [13, 70, 71] it would be interesting to further investigate the role of these mechanisms in mGluR1a activation of TRPC1 in O/A INs.
O/A INs express an mGluR1a-dependent Hebbian form of LTP at their excitatory synapses [5, 8, 9]. This LTP requires cytoplasmic calcium elevation . In O/A INs, cytoplasmic calcium elevation following mGluR1a activation arises from two different sources: entry through plasma membrane TRP channels and release from intracellular stores . The former is the main component of mGluR1a-mediated calcium transients and is required for LTP induction. Slow mGluR1a-mediated EPSCs are thus likely to participate in LTP induction by allowing calcium influx through TRP channels. This mechanism of induction seems to be cell type-specific, as interneurons of stratum radiatum and lacunosum-moleculare, which only display minimal mGluR1a-mediated EPSCs and calcium transients [44, 73], have different mechanisms of LTP induction [9, 74]. Here, we show that mGluR1a-mediated slow EPSCs in O/A INS are dependent on TRPC1, uncovering a new role for TRPC1 in O/A IN synaptic transmission, and likely crucial for Hebbian induction of LTP at these synapses.
Trpc1/4/5 triple knock-out mice show impaired hippocampal excitatory synaptic transmission, spatial working memory and cross-frequency coupling in hippocampal networks . In addition, Trpc1−/− mice have impaired spatial working memory and fear conditioning, as well as deficits in theta burst induced LTP and DHPG-induced LTD at Schaffer collateral-CA1 synapses . These studies demonstrate the importance of TRPC1 and other TRPC members in regulating hippocampal synaptic network and behaviour. Given our evidence of the role of TRPC1 in excitatory synaptic transmission in inhibitory interneurons, it is interesting to speculate that some of these synaptic and behavioural effects may involve TRPCs in inhibitory neurons, possibly O/A interneurons. Hebbian mGluR1a-mediated LTP at excitatory synapses onto O/A INs, and more specifically onto interneurons that express somatostatin (SOM INs), was shown to upregulate persistently LTP magnitude at Schaffer collateral-CA1 pyramidal cell synapses via a disinhibition mechanism [5, 11, 75, 76]. Moreover, impairing mGluR1a-mediated LTP by interfering with mTORC1 function specifically in somatostatin interneurons impairs hippocampal spatial and contextual fear memory consolidation [11, 77, 78]. Thus since mGluR1a-mediated slow excitatory transmission in O/A INs is dependent on TRPC1 and that TRP channels blockade prevents LTP induction in O/A INs , TRPC1 function in interneurons may be important for hippocampal-dependent learning and memory.
In conclusion, we identified that TRPC1 mediates slow excitatory synaptic transmission at O/A INs synapses. Using patch-clamp recordings, we found that slow EPSCs in O/A INs are dependent on TRP channels but may be independent of phospholipase C. Using RT-PCR, we found that mRNA for TRPC 1, 3–7 was present in CA1 hippocampus, but using single-cell RT-PCR, we found expression of mRNA for TRPC 1, 4–7, but not TRPC3, in O/A INs. Using co-immunoprecipitation assays in HEK-293 cell expression system, we found that TRPC1 and TRPC4 interacted with mGluR1a. Co-immunoprecipitation in hippocampus showed that TRPC1 interacted with mGluR1a in vivo. Using immunofluorescence, we found that TRPC1 co-localized with mGluR1a in O/A IN dendrites, whereas TRPC4 localization appeared limited to O/A IN cell body. Finally, down-regulation of TRPC1, but not TRPC4, expression in O/A INs using small interfering RNAs prevented slow EPSCs, suggesting that TRPC1 is an obligatory TRPC subunit for these EPSCs. Our findings uncover a functional role of TRPC1 in mGluR1a-mediated slow excitatory synaptic transmission in O/A INs, suggesting an involvement of TRPC1 in induction of Hebbian LTP at these synapses.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Excitatory postsynaptic current
Human embryonic kidney 293 cells
High frequency stimulation
Long Term Potentiation
Metabotropic glutamate receptor 1a
- O/A INs:
Reverse transcription polymerase chain reaction
Small interfering RNA
- SOM INs:
Transient receptor potential
Canonical transient receptor potential
Klausberger T, Somogyi P. Neuronal diversity and temporal dynamics: the unity of hippocampal circuit operations. Science. 2008;321(5885):53–7.
Pelkey KA, Chittajallu R, Craig MT, Tricoire L, Wester JC, McBain CJ. Hippocampal GABAergic inhibitory interneurons. Physiol Rev. 2017;97(4):1619–747.
Kullmann DM, Lamsa KP. Long-term synaptic plasticity in hippocampal interneurons. Nat Rev Neurosci. 2007;8(9):687–99.
Castillo PE, Younts TJ, Chavez AE, Hashimotodani Y. Endocannabinoid signaling and synaptic function. Neuron. 2012;76(1):70–81.
Vasuta C, Artinian J, Laplante I, Hebert-Seropian S, Elayoubi K, Lacaille JC. Metaplastic Regulation of CA1 Schaffer Collateral Pathway Plasticity by Hebbian MGluR1a-Mediated Plasticity at Excitatory Synapses onto Somatostatin-Expressing Interneurons. eNeuro. 2015;2(4):1–16.
Bezaire MJ, Soltesz I. Quantitative assessment of CA1 local circuits: knowledge base for interneuron-pyramidal cell connectivity. Hippocampus. 2013;23(9):751–85.
Croce A, Pelletier JG, Tartas M, Lacaille JC. Afferent-specific properties of interneuron synapses underlie selective long-term regulation of feedback inhibitory circuits in CA1 hippocampus. J Physiol. 2010;588(Pt 12):2091–107.
Booker SA, Loreth D, Gee AL, Watanabe M, Kind PC, Wyllie DJA, et al. Postsynaptic GABABRs inhibit L-type calcium channels and abolish long-term potentiation in hippocampal Somatostatin interneurons. Cell Rep. 2018;22(1):36–43.
Perez Y, Morin F, Lacaille JC. A hebbian form of long-term potentiation dependent on mGluR1a in hippocampal inhibitory interneurons. Proc Natl Acad Sci U S A. 2001;98(16):9401–6.
Lapointe V, Morin F, Ratte S, Croce A, Conquet F, Lacaille JC. Synapse-specific mGluR1-dependent long-term potentiation in interneurones regulates mouse hippocampal inhibition. J Physiol. 2004;555(Pt 1):125–35.
Artinian J, Jordan A, Khlaifia A, Honore E, La Fontaine A, Racine AS, et al. Regulation of hippocampal memory by mTORC1 in somatostatin interneurons. J Neurosci. 2019;39(43):8439–56.
Huang YH, Sinha SR, Tanaka K, Rothstein JD, Bergles DE. Astrocyte glutamate transporters regulate metabotropic glutamate receptor-mediated excitation of hippocampal interneurons. J Neurosci. 2004;24(19):4551–9.
Topolnik L, Azzi M, Morin F, Kougioumoutzakis A, Lacaille JC. mGluR1/5 subtype-specific calcium signalling and induction of long-term potentiation in rat hippocampal oriens/alveus interneurones. J Physiol. 2006;575(Pt 1):115–31.
Funayama M, Goto K, Kondo H. Cloning and expression localization of cDNA for rat homolog of TRP protein, a possible store-operated calcium (Ca2+) channel. Brain Res Mol Brain Res. 1996;43(1–2):259–66.
Mori Y, Takada N, Okada T, Wakamori M, Imoto K, Wanifuchi H, et al. Differential distribution of TRP Ca2+ channel isoforms in mouse brain. Neuroreport. 1998;9(3):507–15.
Philipp S, Hambrecht J, Braslavski L, Schroth G, Freichel M, Murakami M, et al. A novel capacitative calcium entry channel expressed in excitable cells. EMBO J. 1998;17(15):4274–82.
Strubing C, Krapivinsky G, Krapivinsky L, Clapham DE. TRPC1 and TRPC5 form a novel cation channel in mammalian brain. Neuron. 2001;29(3):645–55.
Martinez-Galan JR, Verdejo A, Caminos E. TRPC1 channels are expressed in pyramidal neurons and in a subset of Somatostatin interneurons in the rat Neocortex. Front Neuroanat. 2018;12:15.
Li M, Chen C, Zhou Z, Xu S, Yu Z. A TRPC1-mediated increase in store-operated Ca2+ entry is required for the proliferation of adult hippocampal neural progenitor cells. Cell Calcium. 2012;51(6):486–96.
Kim SJ. TRPC3 channel underlies cerebellar long-term depression. Cerebellum. 2013;12(3):334–7.
Neuner SM, Wilmott LA, Hope KA, Hoffmann B, Chong JA, Abramowitz J, et al. TRPC3 channels critically regulate hippocampal excitability and contextual fear memory. Behav Brain Res. 2015;281:69–77.
Broker-Lai J, Kollewe A, Schindeldecker B, Pohle J, Nguyen Chi V, Mathar I, et al. Heteromeric channels formed by TRPC1, TRPC4 and TRPC5 define hippocampal synaptic transmission and working memory. EMBO J. 2017;36(18):2770–89.
Griesi-Oliveira K, Suzuki AM, Muotri AR. TRPC channels and mental disorders. Adv Exp Med Biol. 2017;976:137–48.
Trebak M, Lemonnier L, Smyth JT, Vazquez G, Putney JW Jr. Phospholipase C-coupled receptors and activation of TRPC channels. Handb Exp Pharmacol. 2007;179:593–614.
Abramowitz J, Birnbaumer L. Physiology and pathophysiology of canonical transient receptor potential channels. FASEB J. 2009;23(2):297–328.
Bollimuntha S, Selvaraj S, Singh BB. Emerging roles of canonical TRP channels in neuronal function. Adv Exp Med Biol. 2011;704:573–93.
Lepannetier S, Gualdani R, Tempesta S, Schakman O, Seghers F, Kreis A, et al. Activation of TRPC1 channel by metabotropic glutamate receptor mGluR5 modulates synaptic plasticity and spatial working memory. Front Cell Neurosci. 2018;12:318.
Tozzi A, Bengtson CP, Longone P, Carignani C, Fusco FR, Bernardi G, et al. Involvement of transient receptor potential-like channels in responses to mGluR-I activation in midbrain dopamine neurons. Eur J Neurosci. 2003;18(8):2133–45.
Kim SJ, Kim YS, Yuan JP, Petralia RS, Worley PF, Linden DJ. Activation of the TRPC1 cation channel by metabotropic glutamate receptor mGluR1. Nature. 2003;426(6964):285–91.
Bengtson CP, Tozzi A, Bernardi G, Mercuri NB. Transient receptor potential-like channels mediate metabotropic glutamate receptor EPSCs in rat dopamine neurones. J Physiol. 2004;555(Pt 2):323–30.
Faber ES, Sedlak P, Vidovic M, Sah P. Synaptic activation of transient receptor potential channels by metabotropic glutamate receptors in the lateral amygdala. Neuroscience. 2006;137(3):781–94.
Hartmann J, Dragicevic E, Adelsberger H, Henning HA, Sumser M, Abramowitz J, et al. TRPC3 channels are required for synaptic transmission and motor coordination. Neuron. 2008;59(3):392–8.
Kubota H, Nagao S, Obata K, Hirono M. mGluR1-mediated excitation of cerebellar GABAergic interneurons requires both G protein-dependent and Src-ERK1/2-dependent signaling pathways. PLoS One. 2014;9(9):e106316.
Martorana A, Giampa C, DeMarch Z, Viscomi MT, Patassini S, Sancesario G, et al. Distribution of TRPC1 receptors in dendrites of rat substantia nigra: a confocal and electron microscopy study. Eur J Neurosci. 2006;24(3):732–8.
Tian J, Zhu MX. GABAB Receptors Augment TRPC3-Mediated Slow Excitatory Postsynaptic Current to Regulate Cerebellar Purkinje Neuron Response to Type-1 Metabotropic Glutamate Receptor Activation. Cells. 2018;7(8):1–14.
Liman ER, Corey DP, Dulac C. TRP2: a candidate transduction channel for mammalian pheromone sensory signaling. Proc Natl Acad Sci U S A. 1999;96(10):5791–6.
Moran MM, Xu H, Clapham DE. TRP ion channels in the nervous system. Curr Opin Neurobiol. 2004;14(3):362–9.
von Bohlen Und Halbach O, Hinz U, Unsicker K, Egorov AV. Distribution of TRPC1 and TRPC5 in medial temporal lobe structures of mice. Cell Tissue Res. 2005;322(2):201–6.
Chung YH, Sun Ahn H, Kim D, Hoon Shin D, Su Kim S, Yong Kim K, et al. Immunohistochemical study on the distribution of TRPC channels in the rat hippocampus. Brain Res. 2006;1085(1):132–7.
Fowler MA, Sidiropoulou K, Ozkan ED, Phillips CW, Cooper DC. Corticolimbic expression of TRPC4 and TRPC5 channels in the rodent brain. PLoS One. 2007;2(6):e573.
Zechel S, Werner S. von Bohlen Und Halbach O. distribution of TRPC4 in developing and adult murine brain. Cell Tissue Res. 2007;328(3):651–6.
Bourdeau ML, Morin F, Laurent CE, Azzi M, Lacaille JC. Kv4.3-mediated A-type K+ currents underlie rhythmic activity in hippocampal interneurons. J Neurosci. 2007;27(8):1942–53.
Gee CE, Lacaille JC. Group I metabotropic glutamate receptor actions in oriens/alveus interneurons of rat hippocampal CA1 region. Brain Res. 2004;1000(1–2):92–101.
Woodhall G, Gee CE, Robitaille R, Lacaille JC. Membrane potential and intracellular Ca2+ oscillations activated by mGluRs in hippocampal stratum oriens/alveus interneurons. J Neurophysiol. 1999;81(1):371–82.
Vennekens R, Menigoz A, Nilius B. TRPs in the brain. Rev Physiol Biochem Pharmacol. 2012;163:27–64.
Nilius B, Szallasi A. Transient receptor potential channels as drug targets: from the science of basic research to the art of medicine. Pharmacol Rev. 2014;66(3):676–814.
Riccio A, Li Y, Tsvetkov E, Gapon S, Yao GL, Smith KS, et al. Decreased anxiety-like behavior and Galphaq/11-dependent responses in the amygdala of mice lacking TRPC4 channels. J Neurosci. 2014;34(10):3653–67.
Baude A, Nusser Z, Roberts JD, Mulvihill E, McIlhinney RA, Somogyi P. The metabotropic glutamate receptor (mGluR1 alpha) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immunogold reaction. Neuron. 1993;11(4):771–87.
Lebeau G, Maher-Laporte M, Topolnik L, Laurent CE, Sossin W, Desgroseillers L, et al. Staufen1 regulation of protein synthesis-dependent long-term potentiation and synaptic function in hippocampal pyramidal cells. Mol Cell Biol. 2008;28(9):2896–907.
Strubing C, Krapivinsky G, Krapivinsky L, Clapham DE. Formation of novel TRPC channels by complex subunit interactions in embryonic brain. J Biol Chem. 2003;278(40):39014–9.
Miller M, Shi J, Zhu Y, Kustov M, Tian JB, Stevens A, et al. Identification of ML204, a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels. J Biol Chem. 2011;286(38):33436–46.
Schleifer H, Doleschal B, Lichtenegger M, Oppenrieder R, Derler I, Frischauf I, et al. Novel pyrazole compounds for pharmacological discrimination between receptor-operated and store-operated Ca(2+) entry pathways. Br J Pharmacol. 2012;167(8):1712–22.
Valero ML, Caminos E, Juiz JM, Martinez-Galan JR. TRPC1 and metabotropic glutamate receptor expression in rat auditory midbrain neurons. J Neurosci Res. 2015;93(6):964–72.
Clapham DE, Runnels LW, Strubing C. The TRP ion channel family. Nat Rev Neurosci. 2001;2(6):387–96.
Goel M, Sinkins WG, Schilling WP. Selective association of TRPC channel subunits in rat brain synaptosomes. J Biol Chem. 2002;277(50):48303–10.
Tempia F, Miniaci MC, Anchisi D, Strata P. Postsynaptic current mediated by metabotropic glutamate receptors in cerebellar Purkinje cells. J Neurophysiol. 1998;80(2):520–8.
Tozzi A, Guatteo E, Caputi L, Bernardi G, Mercuri NB. Group I mGluRs coupled to G proteins are regulated by tyrosine kinase in dopamine neurons of the rat midbrain. J Neurophysiol. 2001;85(6):2490–7.
Canepari M, Ogden D. Evidence for protein tyrosine phosphatase, tyrosine kinase, and G-protein regulation of the parallel fiber metabotropic slow EPSC of rat cerebellar Purkinje neurons. J Neurosci. 2003;23(10):4066–71.
Huang GN, Zeng W, Kim JY, Yuan JP, Han L, Muallem S, et al. STIM1 carboxyl-terminus activates native SOC, I(crac) and TRPC1 channels. Nat Cell Biol. 2006;8(9):1003–10.
Yuan JP, Zeng W, Huang GN, Worley PF, Muallem S. STIM1 heteromultimerizes TRPC channels to determine their function as store-operated channels. Nat Cell Biol. 2007;9(6):636–45.
Ong HL, Cheng KT, Liu X, Bandyopadhyay BC, Paria BC, Soboloff J, et al. Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components. J Biol Chem. 2007;282(12):9105–16.
Zhang YH, Wu HJ, Che H, Sun HY, Cheng LC, Li X, et al. Functional transient receptor potential canonical type 1 channels in human atrial myocytes. Pflugers Arch. 2013;465(10):1439–49.
Ong HL, Ambudkar IS. Molecular determinants of TRPC1 regulation within ER-PM junctions. Cell Calcium. 2015;58(4):376–86.
Ambudkar IS, de Souza LB, Ong HL. TRPC1, Orai1, and STIM1 in SOCE: friends in tight spaces. Cell Calcium. 2017;63:33–9.
Boulay G, Brown DM, Qin N, Jiang M, Dietrich A, Zhu MX, et al. Modulation of Ca(2+) entry by polypeptides of the inositol 1,4, 5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP): evidence for roles of TRP and IP3R in store depletion-activated Ca(2+) entry. Proc Natl Acad Sci U S A. 1999;96(26):14955–60.
Kiselyov K, Mignery GA, Zhu MX, Muallem S. The N-terminal domain of the IP3 receptor gates store-operated hTrp3 channels. Mol Cell. 1999;4(3):423–9.
Kiselyov KI, Shin DM, Wang Y, Pessah IN, Allen PD, Muallem S. Gating of store-operated channels by conformational coupling to ryanodine receptors. Mol Cell. 2000;6(2):421–31.
Yuan JP, Kiselyov K, Shin DM, Chen J, Shcheynikov N, Kang SH, et al. Homer binds TRPC family channels and is required for gating of TRPC1 by IP3 receptors. Cell. 2003;114(6):777–89.
Worley PF, Zeng W, Huang G, Kim JY, Shin DM, Kim MS, et al. Homer proteins in Ca2+ signaling by excitable and non-excitable cells. Cell Calcium. 2007;42(4–5):363–71.
Hartmann J, Karl RM, Alexander RP, Adelsberger H, Brill MS, Ruhlmann C, et al. STIM1 controls neuronal Ca(2)(+) signaling, mGluR1-dependent synaptic transmission, and cerebellar motor behavior. Neuron. 2014;82(3):635–44.
O'Riordan K, Gerstein H, Hullinger R, Burger C. The role of Homer1c in metabotropic glutamate receptor-dependent long-term potentiation. Hippocampus. 2014;24(1):1–6.
Ouardouz M, Lacaille JC. Mechanisms of selective long-term potentiation of excitatory synapses in stratum oriens/alveus interneurons of rat hippocampal slices. J Neurophysiol. 1995;73(2):810–9.
Carmant L, Woodhall G, Ouardouz M, Robitaille R, Lacaille JC. Interneuron-specific Ca2+ responses linked to metabotropic and ionotropic glutamate receptors in rat hippocampal slices. Eur J Neurosci. 1997;9(8):1625–35.
Lamsa K, Heeroma JH, Kullmann DM. Hebbian LTP in feed-forward inhibitory interneurons and the temporal fidelity of input discrimination. Nat Neurosci. 2005;8(7):916–24.
Leao RN, Mikulovic S, Leao KE, Munguba H, Gezelius H, Enjin A, et al. OLM interneurons differentially modulate CA3 and entorhinal inputs to hippocampal CA1 neurons. Nat Neurosci. 2012;15(11):1524–30.
Artinian J, Lacaille JC. Disinhibition in learning and memory circuits: new vistas for somatostatin interneurons and long-term synaptic plasticity. Brain Res Bull. 2018;141:20–6.
Ran I, Laplante I, Bourgeois C, Pepin J, Lacaille P, Costa-Mattioli M, et al. Persistent transcription- and translation-dependent long-term potentiation induced by mGluR1 in hippocampal interneurons. J Neurosci. 2009;29(17):5605–15.
Ran I, Laplante I, Lacaille JC. CREB-dependent transcriptional control and quantal changes in persistent long-term potentiation in hippocampal interneurons. J Neurosci. 2012;32(18):6335–50.
This work was funded by a grant from the Canadian Institutes of Health Research to J.-C.L. (Project grant PJT-153311). J.-C.L. is the recipient of the Canada Research Chair in Cellular and Molecular Neurophysiology. J.G.P. was supported by a postdoctoral fellowship from Fonds de la Recherche du Québec Santé, and A.Ko. by a bursary from the Natural Sciences and Engineering Research Council of Canada.
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Kougioumoutzakis, A., Pelletier, J.G., Laplante, I. et al. TRPC1 mediates slow excitatory synaptic transmission in hippocampal oriens/alveus interneurons. Mol Brain 13, 12 (2020). https://doi.org/10.1186/s13041-020-0558-9
- GABA interneurons
- TRPC subtypes
- mGluR1a-mediated slow EPSC